isolation of stromal stem cells from human adipose tissue pdf
Báo cáo khoa học: "In vitro neuronal and osteogenic differentiation of mesenchymal stem cells from human umbilical cord blood" ppt
... tnecseroulf gniwollof ehT SBP htiw semit eerht desnir erew selpmas ehT Co73 ta h rof sllec eht htiw detabucni erew seidobitna yramirp ehT ylevitcepser ,PAFG rof 002 : dna ESN rof 002 : ta ares ... 001-X notirT %4.0 neht dna ,nim rof enoteca %001 ,nim 01 rof lonahtem %001 dloc eci htiw detaert neht erew yehT erutarepmet moor ta nim 01 rof SBP ni edyhedlamrof %7.3 htiw dexif neht dna SBP ... llec gnimrofrep dna esu lacinilc rof SBF gnisu tuohtiw oviv xe sCSM dnapxe ot sdohtem dnif ot deen ew dna ,hcraeser ruo rof SBF desu ew ,yduts tneserp ni ,revewoH noitarefilorp sCSM rof noitidnoc...
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Báo cáo y học: "Identification and isolation of embryonic stem cells in reproductive endocrinology: theoretical protocols for conservation of human embryos derived from in vitro fertilization" ppsx
... transfer [2] Homogenous human ESC colonies may be derived from subsequent isolation and re-plating of the ICM cells, which are then screened for stemness by a variety of recognized markers Once ... stage, a human embryo at 2–3d post fertilization consists of just 4–8 cells, all of which are totipotent In contrast to the pluripotent cells obtained from the blastocyst ICM, one or two of these ... death and the creation of human embryonic stem cells J Clin Invest 2004, 114:1184-1186 Voullaire L, Slater H, Williamson R, Wilton L: Chromosome analysis of blastomeres from human embryos by using...
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Báo cáo y học: "Identification of subpopulations with characteristics of mesenchymal progenitor cells from human osteoarthritic cartilage using triple staining for cell surface markers" docx
... HL, Lo WH: Isolation and characterization of size-sieved stem cells from human bone marrow Stem Cells 2002, 20:249-258 Shur I, Marom R, Lokiec F, Socher R, Benayahu D: Identification of cultured ... migration of primary human mesenchymal progenitor cells J Cell Biochem 2002, 87:305-312 Cheng SL, Yang JW, Rifas L, Zhang SF, Avioli LV: Differentiation of human bone marrow osteogenic stromal cells ... functionality of cell surface molecules on human mesenchymal stem cells J Biomed Sci 2003, 10:228-241 Reyes M, Verfaillie CM: Characterization of multipotent adult progenitor cells, a subpopulation of mesenchymal...
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Báo cáo y học: "Adipose-derived mesenchymal stem cells from the sand rat: transforming growth factor beta and 3D co-culture with human disc cells stimulate proteoglycan and collagen type I rich extracellular matrix" ppsx
... synthesis of proteoglycans after pellet co-culture of disc cells with BM-MSC [20] Stimulation of disc cells with stem cells or CM could enhance the success of autologous implantation of disc cells ... annulus cells adiposederived mesenchymal stem of AD-MSCfor 3D co cultured alone Increase in separate culture cells (AD-MSC) and annulus cells comderived mesenchymal stem cells (AD-MSC) and annulus cells ... Chondrogenic potential of adipose tissue- derived stromal cells in vitro and in vivo Biochem Biophys Res Commun 2002, 290:763-769 Gimble JM, Guilak F: Adipose- derived adult stem cells: isolation, characterization,...
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Differentiation and derivation of lineage committed chondroprogenitors and chondrogenic cells from human embryonic stem cells for cartilage tissue engineering and regeneration
... temporary relief of symptoms Stem cells represent a promising cell source for cartilage repair and can be derived from two major sources: mesenchymal stem cells (MSCs) and embryonic stem cells (ESCs) ... expansion of the differentiated cells, phenotypic stability of differentiated cells and tissues, delivery systems and biomaterials for cartilage repair, and survivability and integration of the tissue ... chondrogenic cells ………………………… 83 PHASE III: ISOLATION OF CHONDROGENIC CELLS …………… 86 4.3.1 4.3.2 Expansion of hESC-derived chondrogenic cells …………………88 4.3.3 Differentiation capability of hESC-derived...
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Tài liệu Báo cáo khoa học: Characterization of 1H NMR detectable mobile lipids in cells from human adenocarcinomas doc
... 1 H NMR of mobile lipids in tumour cells A M Luciani et al MCF-7 cells from human cancers In a previous study [13], we demonstrated that these cells display intensity modulation of ML signals ... spectra of extracted lipids (A) and PCA extracts (AÂ) from one representative sample of MCF-7 cells; 2D 1H NMR spectra of extracted lipids (B) and PCA extracts (BÂ) from one representative sample of ... pattern of the same region (C) and of the reference signal (CÂ) (p.p.m.) Table Mean values of parameters d (p.p.m.), Dm (Hz) and IR after deconvolution of 1D spectra from PCA extracts of HeLa cells...
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Báo cáo hóa học: " Nutraceutical augmentation of circulating endothelial progenitor cells and hematopoietic stem cells in human subjects" potx
... proliferation of human bone marrow cells was assessed, in which a 60% enhancement of growth was reported [76] Bone marrow cells from mice supplemented with Nutra -Stem were protected from in vitro ... feasibility of significant mobilization of cells expressing hematopoietic stem cell and EPC markers and properties The area of nutritional modulation of the stem cell compartment offers significant ... proliferation of human stem cells Stem Cells Dev 2006, 15(1):118-23 77 Shytle RD, Ehrhart J, Tan J, Vila J, Cole M, Sanberg CD, Sanberg PR, Bickford PC: Oxidative stress of neural, hematopoietic, and stem...
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Báo cáo sinh học: " Phosphoproteomic analysis of apoptotic hematopoietic stem cells from hemoglobin E/b-thalassemia" pot
... the isolation of relatively pure populations of these cells for in vitro study of the cellular processes in thalassemic stem cells While previous attempts have focused on the cell culture system, ... culture is capable of sustaining a large number of cells, which can confer specific advantages and disadvantages The use of freshly isolated human hematopoietic stem cells from thalassemic patients ... The isolated HSCs/CD34+ cells were harvested for phosphoproteomic analysis and cultivation of erythroid cells The cultivation of erythroid cells derived from HSCs/CD34+ cells was performed according...
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o cáo hóa học:" Phosphoproteomic analysis of apoptotic hematopoietic stem cells from hemoglobin E/b-thalassemia" pptx
... the isolation of relatively pure populations of these cells for in vitro study of the cellular processes in thalassemic stem cells While previous attempts have focused on the cell culture system, ... culture is capable of sustaining a large number of cells, which can confer specific advantages and disadvantages The use of freshly isolated human hematopoietic stem cells from thalassemic patients ... The isolated HSCs/CD34+ cells were harvested for phosphoproteomic analysis and cultivation of erythroid cells The cultivation of erythroid cells derived from HSCs/CD34+ cells was performed according...
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Báo cáo y học: "Hypoxic conditions increase hypoxia-inducible transcription factor 2α and enhance chondrogenesis in stem cells from the infrapatellar fat pad of osteoarthritis patients" pps
... access, cells from the IPFP are of interest as a potential source of cells for the repair of focal cartilage defects in the knee [5] In previous work we demonstrated the ability of IPFPderived cells ... day 14 The yield of cells by the end of passage was typically 107 cells at confluence from g of IPFP tissue (proliferation rate 0.14 ± 0.01 doublings per day (mean ± SEM) [8]) Cells at passage ... GAG per cell These results with IPFP cells are comparable to those from a previous study on stem cells derived from other human liposuction-derived adipose tissue, in which 5% oxygen was reported...
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Báo cáo y học: "Primary T-cells from human CD4/CCR5-transgenic rats support all early steps of HIV-1 replication including integration, but display impaired viral gene expression" doc
... of hCD4 were similar, but levels of hCCR5 were markedly higher on CD4 T -cells from transgenic rats than on those from their human counterparts ([11] and data not shown) The percentages of T -cells ... expression of human Cyclin T1 in primary rat TTransient expression of human Cyclin T1 in primary rat T -cells boosts early HIV gene expression (A) Sequence alignment of amino acids 250–290 of human, ... 34395) and supervised by animal welfare officers of the University of Heidelberg Cells Cultures of primary T -cells and macrophages from randomly selected human donors and transgenic rats and BALB/c...
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Characterization of human adipose tissue derived adult multipotent precursor cells
... use of stem cells in regeneration of bone 1-1 1-1 1-2 Chapter – Literature Review 2.1 Description of the adipose tissue 2.2 Adipose tissue – a possible alternative to bone marrow as a source of ... that cells actually thrive in One of the promising adult stem cell sources is a culture plastic-adherent cell population from the human adipose tissue In order to bring adipose derived stem cells ... POTENTIAL OF HUMAN ADIPOSE TISSUE DERIVED STEM CELLS ISOLATED FROM TISSUE ASPIRATED WITH A VACUUM PUMP OR SYRINGE 5.1 CHAPTER ABSTRACT The first step in working on ADSC is the proper procurement of adipose...
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Generation of hepatocyte like cells from mouse embryonic stem (ES) and bone marrow (BM) cells
... Derivation of human EG cells from aborted fetus has also been reported (Shamblott, Axelman et al 1998) The third type of stem cell is the adult stem cells Like ES and EG cells, adult stem cells can ... Generation of Hepatocyte-like cells from Mouse Embryonic Stem (ES) and Bone Marrow (BM) Cells YIN YIJUN A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY OF MEDICINE DEPARTMENT OF OBSTETRICS ... Itskovitz-Eldor et al 1998; Reubinoff, Pera et al 2000) The second type of stem cell is embryonic germ (EG) cells isolated from the primordial germ cells of the gonadal ridge of the fetus (Matsui, Zsebo...
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The derivation, propagation, storage and gene expression of human embryonic stem cells on human feeders
... categories of cells make up the human body viz., germ cells, somatic cells and stem cells Somatic cells include the bulk of the cells that make up the human adult and each of these cells in their ... hematopoietic stem cells, mesechymal stem cells and neuronal stem cells Adult stem cells have also been isolated from several other organs such as the brain (neuronal stem cells) , skin (epidermal stem cells) , ... collected from human MAPCs as most of the current experimental data are derived from studies in the murine model Classification of stem cells Germ cells of the gonads Mammalian stem cells are...
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Stem Cells in Human Reproduction Basic Science and Therapeutic Potential Second Edition pot
... differentiation of embryonic stem cells into ovarian structures containing oocytes Stem Cells 2006; 24:266–273 24 Qing T, Shi Y, Qin H, et al Induction of oocyte-like cells from mouse embryonic stem cells ... germline potential of stem cells derived from nongonadal tissues, specifically fetal porcine skin, is also presented by Julang Li The controversial issue of the existence of germline stem cells in adult ... of adult human mesenchymal stem cells Science 1999; 284:143–147 Goodman JW, Hodgson GS Evidence for stem cells in the peripheral blood of mice Blood 1962; 19:702–714 Gamete Generation from Stem...
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Báo cáo khoa học: Epigenetics: the study of embryonic stem cells by restriction landmark genomic scanning pptx
... study of embryonic stem cells TE N Hattori and K Shiota ICM PGC TS cells Placental cells 77 ES cells 49 EG cells Embryonic cells Fig Epigenetic distances between ES cells and other stem cells ... apparent ED between ES cells and EG cells, the ED of TS cells to ES cells (77) is greater than that of EG cells to ES cells (49), confirming the similarity of EG cells to ES cells DNA methylation ... profiles Epigenetic study of embryonic stem cells Differences in DNA methylation profiles between ES and other stem cells Comparing ES cells with other stem cells established from developing embryos...
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Báo cáo khoa học: The heterogeneity of mast cell tryptase from human lung and skin Differences in size, charge and substrate affinity ppt
... the purity of mast cells thus obtained ranged from 65% to 95% of all nucleated cells Isolation of skin mast cells Mast cells were isolated as described previously from infant foreskin tissue obtained ... kinetics of cleavage of a range of chromogenic substrates Materials and methods Isolation of lung mast cells Human lung mast cells were isolated as described previously [39] Briey, cells from macroscopically ... relative proportions of the two enzymes.) pH prole Fig Mathematical modeling of the behavior of a mixture of isoforms of an enzyme (A) Hanes plot of a theoretical mixture of isoforms of an enzyme for...
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differentiation of embryonic stem cells
... TABLE OF CONTENTS Development of Hematopoietic Repopulating Cells from Embryonic Stem Cells The In Vitro Differentiation of Mouse Embryonic Stem Cells into Neutrophils 10 Development and Analysis of ... Megakaryocytes from Murine Embryonic Stem Cells 11 Development of Lymphoid Lineages Embryonic Stem Cells In Vitro from 12 Probing Dendritic Cell Function by Guiding the Differentiation of Embryonic Stem Cells ... Embryonic Stem Cells YIJING CHEN, JAY L VIVIAN, AND TERRY MAGNUSON 406 Section IV Differentiation of Monkey and Human Embryonic Stem Cells 29 Growth and Differentiation Monkey ES Cells of Cynomolgus...
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báo cáo hóa học:" Implantation of neural stem cells embedded in hyaluronic acid and collagen composite conduit promotes regeneration in a rabbit facial nerve injury model" doc
... immunohistochemical staining of BrdU and S-100 Representative images of immunohistochemical staining of BrdU and S-100 A: Localization of darkly brownish stained BrdU+ cells to transected tissues of rabbits having ... light microscopy of NSC in implanted tex of E16 Sprague-Dawley rat embryos culture on of neural stem cell (NSC) at passage three derived from the composite Representative image of scanning electron ... in tissues of rabbits with facial nerve fiber transection and implant of NSC and HA-collagen scaffold for 12 weeks (400× magnification) C: An array of fascicles of relatively uniform size in tissues...
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