... perform complex tasks In addition, for developmental studies, there may be task performance difference between groups, e.g simple tasks for adults could be hard for children, and simple tasks for ... resolution 3D structural image (Figure 2.6, Left) These structural images can provide detailed information of the brain’s shape and size, and provide the base for cortical surface reconstruction and segmentation ... structural MRI andfunctional MRI (fMRI), is an important non-invasive technique for diagnosing and treating medical conditions Structural MRI can provide good contrast of gray/white matter and...
... Council for Environment, Agricultural Sciences and Spatial Planning (to L.W and S.E.), the 4928 Swedish Research Council (to H.E and P.N.) and the Swedish Cancer Foundation (to H.E and P.N.) The Structural ... observed for hypoxanthine and guanine (Table 1) These results imply that PRPP is required for the nucleobases to bind For comparison the DSLS thermal melt assay was run for HPRT but, unfortunately ... thermal-melt assay PRPP, IMP and GMP were the top hits and therefore were identified as potential substrates, products or regulatory ligands for PRTFDC1 The catalytic efficiency and substrate specificity...
... between R81 and P82 and between G90 and I91 A similar cis peptide between G93 and V94 is found in Tm SurE also Overall structural features of the protein St SurE is an aba sandwich protein and adopts ... contrast, the AD and AC interfaces of the monoclinic form bury only 419 and ˚ 902 A2 respectively These buried areas are not sufficient for tight association Gel filtration analysis and dynamic light-scattering ... required for phosphorus scavenging and remobilization when the cells are under stress and consequently mononucleotide phosphate concentrations are high 5862 Materials and methods Cloning and purification...
... 1.5 for U937 and 1.2 for A549 cells, suggesting positive ligand–receptor cooperativity The numbers of binding sites per cell calculated for peptide 11024 were 200 000 and 000 000 for U937 and ... nm) (Fig 5A) Scatchard and Hill analyses were then performed and dissociation constants (Kd) were calculated for Rv2560 HABP 11024 for U937 and A549 cell lines (Kd of 380 and 800 nm, respectively) ... Autoradiographs for U937 proteins specifically cross-linked with radiolabelled peptide 11024 Lanes and 2, U937 cells; lanes and 4, HepG2 cells; lanes and 3, total radiolabelled peptide binding; lanes and...
... Graphics workstation and was based on the structures of the human and porcine pancreatic lipases for the open form and on the horse pancreatic lipase for the closed form [16,18,19,30] For homology modeling, ... K414A, and S132T, are presented as specific activities Catalytic activities for the WW mutant (Ala substituted for Trp393 and Trp394) and the WWW mutant (Ala substituted for Trp390, Trp393 and Trp394) ... constructed for the inactive and active forms of LPL reveal interesting features of LPL structure, including the conformational change in the active center, the critical sites for heparin binding, and...
... the same preparations in a native gel run in parallel (1¢, 2¢ and 3¢) Six micrograms of protein was loaded per lane (NIES-44 and HUB5-2-4) also showed an immunodetectable band of c 22 kDa and exhibited ... UÆmg)1 protein and recovery levels of 20– 30% In all cases, the analysis by SDS ⁄ PAGE and native PAGE of purified preparations showed only one protein band of 20–22 kDa (Figs and 2; supplementary ... pyrophosphatases, sPPases and proton-translocating PPases, H+-PPases, with different subcellular localizations, hydrolyze PPi generated by cell anabolism, and replenish the Pi pool needed for phosphorylation...
... mates for the valuable discussion and friendship and the present and former members of Functional Genomics Laboratory as well as Structural Biology Laboratory Special thanks to Lim Daina and Thomas ... 5.3.5 Sequence andstructural homology 101 5.3.6 Functional implications 104 Chapter Summary and Future Studies 107 6.1 Summary 108 6.2 Future studies 109 6.2.1 Establishment of cell line 109 ... important for any further functional or structuralstudies on the protein This is because only folded proteins retain their functional activity and three dimensional structures (Rehm et al., 2002) Unfortunately,...
... Adrian Compoy for analysing the ITC data, A/P Markus Wenk and Dr Aaron Fernandis for mass spectrometry and lipid identification experiments, Prof Ilan Rosenshine and Dr Henry Mok for helping in ... bacterial inner membrane, P stands for bacterial periplasm; OM means bacterial outer membrane, ECM means extracellular milieu and PM (brown zone) stands for host cell plasma membrane Where appropriate, ... translocon is able to handle different types of proteins, in the unfolded form (Holland 2004) SecY protein forms the channel pore and it also forms a complex with SecE and SecG; in addition,...
... T cell dysfunction systemically and affects both the HIV-infected cells and the majority of bystander cells Studies on CD8+ T cells are limited, and include searching for genes responsible for ... blue dot A Volcano plot for CD4+ T cells between BDL and LTNP groups B Volcano plot for CD8+ T cells between BDL and LTNP groups C Volcano plot for CD8+ T cells between VIR and LTNP groups D Plot ... performed on separated CD4+ and CD8+ T cell populations demonstrated that 99.2% ± 0.165 (mean ±SD) and 99.1% ± 0.128 (mean ±SD) of purified CD4+ and CD8+ cells were single positive for the CD4 and...