... order conditions, using a substrate concentra-tion far below the Km, as reported elsewhere [13]. Calcula-tions were done usin gENZFITTERsoftware (Biosoft).Excitation and emission wavelengths ... of4Murea and a cocktail of inhibitors. T here was nosigni®cant degradation of semenogelins under these condi-tions, which allow the rapid separation by centrifugation ofcellular components and ... theglutamine±serine bond and the ®ltered fraction at theserine±tyrosine bond, as shown by HPLC fractionation and N-terminal sequencing. Part of the last ®ltered fraction waspuri®ed further...