... 203–206 Kato K, Fujiyama K, Hatanaka HS, Prijambada ID, Negoro S, Urabe I & Okada H (1991) Amino acid alterations essential for increasing the catalytic activity of the nylon-oligomer degradation ... between two atoms in the enzyme–substrate complex are indicated as red dotted lines, with distance in angstroms Substrate Ald was refined as alternative conformations (AldA and AldB) on the basis of ... Shibata N, Takeo M, Negoro S & Higuchi Y (2005) Crystallization and x-ray diffraction analysis of 6-aminohexanoate-dimer hydrolase from Arthrobacter sp KI72 Acta Crystallogr F61, 928–930 Hatanaka...
... phosphate The latter component may be a byproduct of the hydrolysis reaction or a natural contaminant These results indicate that the LPS from B vulgatus mainly contains lipid A carrying four fatty ... spectra, indicating a nonsubstituted Rha The signal of H4 -a was approximately one third the intensity of that of H1 -a or H1-b, indicating its ratio Table NMR data for OPS The spectra were measured ... a GlcN2 backbone witha phosphate and mainly four fatty acids for lipid A, and [fi4 )a- L-Rhap (1fi3 )a- D-Manp(1fi] for the OPS moiety ACKNOWLEDGEMENTS This study was supported in part by a grant from...
... origin of AstV-MLB1 is unclear AstVMLB1 may be a bona fide human virus capable of infecting and replicating within the human gastrointestinal tract that had evaded detection until now Alternately, ... totaling 57 amino acids located within a highly conserved motif near the carboxyl terminus of human astroviruses 1–8 This deletion falls within a 144 aa region that has been mapped as being an ... a non-structural polyprotein which contains a serine-like protease motif Pfam analysis revealed a region of ORF 1a that has homology to a peptidase domain In addition, alignment of AstV-MLB1 with...
... obtained from animals cannot always be extrapolated to humans Bjelle [36] has analysed the mechanical response of human knees and found an increase in glycosaminoglycan productionin loadbearing ... protein analysis The cartilage used for RNA quantification was diced and incubated with RNAlater (Qiagen Inc., Valencia, CA, USA) overnight at 4°C prior to storage Available online http://arthritis-research.com/content/8/5/R149 ... mechanical load areas were obtained from each femoral head The horizontal bar indicates the median, the box is the interquartile range, and the vertical lines indicate the atypical values Median...
... tomography, using 11 C-labeled 5-hydroxytryptophan as the tracer Additional hormonal screening was normal, and analyses of insulin antibodies and sulphonylurea were negative Two intra-arterial calcium ... Page of Figure Regular hematoxylin-eosin and immunohistochemistry stains using antibodies directed against synaptophysin, insulin, glucagon, somatostatin, and pancreatic polypeptide (PP) and action ... nesidioblastosis causing hypoglycemia: an important clinical entity and continuing treatment dilemma Arch Surg 2001, 136:656-663 Tsujino M, Sugiyama T, Nishida K, Takada Y, Takanishi K, Ishizawa M, Hirata Y:...
... data from the physical examination of the patient and PD was a major contributor in writing the manuscript RRG, EG and II performed the analysis and interpretation of data in relation to renal ... renal and liver function, cytogenetic analysis and blood analysis EGA and ALA carried out muscle analysis JCD performed the analysis and interpretation of mitochondrial DNA data and JG and MG analyzed ... following a normal pregnancy history At age years, she noted that her subcutaneous adipose facial tissue gradually began to decrease and she complained of generalized muscle pain, predominantly in...
... field interaction within the dipole and rotating wave approximations Moreover, Vj (j = {1, 2}) correspond 52 ´ W LEONSKI, VAN CAO LONG, THUAN BUI DINH, KHOA DINH XUAN to the configurational interaction ... we apply standard Laplace transform procedure We assume that the system initially was in its ground state |0 , i.e a0 = and b1 = b2 = bE = for the time t = Thus, after eliminating the probability ... separation and heights increase as the value of ΓL decreases Usually, such peaks can be observed in the photoelectron spectra when atomic systems interacts with high external fields This fact indicates...
... be large in order to obtain more gain with G than with I at fixation Optimal selection always yielded more gain than truncation selection at a fixed rate of inbreeding A comparison of I with ... table 1! As intended, with optimal selection, the increase in T inbreeding was maintained at the desired constantrate (6.F ;:::j %) over generations and consequently the accumulated inbreeding ... a clear advantage of using the major gene after fixation of the favourable allele although the advantage was higher with BLUP The maximum extra total gains from using the gene were at t andt...
... and technology (KNUST) generates a colossal amount of waste (solid and liquid) The solid waste is dumped at a site far away from the inhabited part of campus and the liquid waste is sent to a ... potential from biogas, the average characteristics of the methane present, the biogas engine efficiency, etc are used as presented in Table Table Electricity potential estimation parameters Parameters ... potential of the sewage at the Primary Sedimentation Tank (PST) at the KNUST sewage treatment plant and its potential power production Feedstock analysis 2.1 Wastewater handling at KNUST Liquid waste...
... tracks changes made to data by maintaining multiple versions of each row allowing the data to be reconciled later to a data source using a DataAdapter The data source to which the DataSet is reconciled ... the destination DataAdapter is called using the DataSet containing the changes as the data object argument; this applies the changes to the destination data source The destination DataSet is ... contains data and schema information within its contained objects, but not information about the provider that was used to retrieve the data or the original source of the data The DataSet tracks...
... cultivated in L normal nutrition medium and incubated batchwisely at 22oC The system was aerated at an air flowrate of L/min with or without the addition of pure CO2 gas The fermentor is agitated ... temperature was investigated in this study Gas chromatography analysis Sample was dissolved in ethyl acetate and 0.5 µL of this was injected into a Shimadzu GC-1 7A (Kyoto, Japan) equipped with flame ionization ... composition and productivity was investigated by varying the CO2 concentration At first, the culture was aerated under air flowrate of L/min without additional CO2 By taking into account the...
... cultivated in L normal nutrition medium and incubated batchwisely at 22oC The system was aerated at an air flowrate of L/min with or without the addition of pure CO2 gas The fermentor is agitated ... temperature was investigated in this study Gas chromatography analysis Sample was dissolved in ethyl acetate and 0.5 µL of this was injected into a Shimadzu GC-1 7A (Kyoto, Japan) equipped with flame ionization ... composition and productivity was investigated by varying the CO2 concentration At first, the culture was aerated under air flowrate of L/min without additional CO2 By taking into account the...
... CP) at a fixed Spezyme CP loading of 10 mg/g total glucan and xylan in the raw biomass and at a fixed total enzyme protein loading of 10 mg/g total glucan and xylan in the raw biomass BSA Treatment ... Certainly, with additional mechanical and manual operation, more cellulosic materials can be recovered from black bin wastes Figures and show how wastes from blue and green 12 bins are separated, ... cans, while that from green bins contains grass and woody wastes Comparing with MSW from black bins, which contains un-sorted residential and commercial wastes, MSW from blue and green bins are...
... marginal tax rate, tax loss carryforwards and investment functions – empirical analysis using a large German panel data set Fred Ramb 26 22 2007 Volatile multinationals? Evidence from the labor ... learning and European inflation dynamics Anke Weber 17 2007 Does intra-firm bargaining matter for business cycle dynamics? Michael Krause Thomas Lubik 18 2007 Uncertainty about perceived inflation ... labor demand of German firms Claudia M Buch Alexander Lipponer 23 2007 International investment positions and Michael Binder exchange rate dynamics: a dynamic panel analysis Christian J Offermanns...
... protein production using 4AaCter T Hayakawa et al Escherichia coli, protein inclusions containing biologically active toxin are formed [3,4] Furthermore, the bipyramidal crystals denatured in m ... of GST–4AaCters in E coli (A) GST–4AaCters expressed in E coli were separated by SDS ⁄ PAGE (15%) and stained with CBB GST–4AaCters are indicated by arrowheads (B) Cells expressing GST–4AaCters ... serum albumin as a standard or by using the densitometric scanning method witha cooled CCD camera system (Ez-Capture; ATTO, Tokyo, Japan) and image analysis software (CS analyser ver 3.0; ATTO,...
... was used ina stratified towing tank and ina neutral wind tunnel The towing tank was 1.2m in depth, 2.4m in width and 25m in length and was stably stratified with layered mixtures of salt water ... plume and analyzing them witha Beckman Model 400 Hydrocarbon Analyzer (a Same ionization detector) in the continuous operating mode A 2-mht sampling time was found to yield reasonably stable values ... the separation point in neutral flow, the neutral -flow concentrations are constant, indicating strong turbulence and mixing in the recirculating region As mentioned above, in the stratified flow, ...
... by a continued increase in viral divergence but witha stabilisation or decline in viral diversity; and a late phase characterized by a stabilisation of viral divergence and a continued stability ... mechanism(s) that are not necessarily associated with changes in coreceptor usage or enhanced replicative capacity in vitro V1V2 and V3 HTA analysis Changes in the dominant viral quasispecies may ... viral fitness However, other studies examining HIV-1 progression in individuals harbouring only R5 variants showed an increase in viral diversity in viral isolates obtained from patients with AIDS...
... Next, a MUAP is obtained by combining the extracellular action potentials of all fibres belonging to one MU This MUAP is convoluted witha pulse train, resulting in the MUAP train for that MU Finally, ... plotted ina three-dimensional graph against the time instant and the scale factor, resulting ina so-called scalogram The algorithm started with calculating the CWT for the first channel When ... with an explained variance of 0.91 (p < 0.001) RMS was best approximated by a square root relation, with an explained variance of 0.92 (p < 0.001) In simulation firing rate was simulated in two...
... breakpoints with GARD [15], and then estimating the dN/dS ratios independently for each fragment Mean dN/dS ratios across races and treatments were compared using ANOVA, linear models (lm) and pairwise ... response may have increased viral rgp120 adaptation in blacks In North America, blacks correspond to 42% of all newly diagnosed HIV/AIDS cases, while white (non-Hispanic) and Hispanic individuals represent ... viral samples isolated from vaccinated and placebo (non-vaccinated) white individuals To test whether levels of selection were significantly different between vaccinated and placebo individuals...