... total volume ofthe column(% 12 min). However, as previously found with otherdissociated capsid proteins, the lack of elution of the proteins can be explained by non-specific binding of the denatured ... resides on the surface ofthe protein and makes noobvious stabilizing interactions with other amino acid side-chains, this part ofthe protein interacts with genomic RNA[33,41,47]. Such interactions ... decrease the stability ofthe capsid. Our findingsilluminate theroleof packing inthe icosahedral lattice of the virus capsid.Experimental proceduresChemicalsAll reagents were of analytical...
... go inthe same direction. The inverse isthecasefortheattractioninthecaseofAnabaena. Also, if the structure ofthe final complex in which electron transfertakes place remained unaltered with increasing ... character-ization ofthe plastocyanins has been described [1].Representations ofthe electrostatic surfaces showing the changes introduced by the mutations are displayed in Fig. 1. The decay ofthe flash-induced ... interaction.Electron-transfer chains like that of oxygenic photosyn-thesis impose special restraints on the proteins involved.Reactions must be fast to allow rapid turnover of the chain. Binding between the reaction...
... generate these values, could be contained. The emotional intensity ofthe backlash against the intrusions of these subaltern groups suggests the importance of readingthese developments in ways ... result of the dissemination of knowledge. D’Israeli was less confident of the effects ofthe invention of Printing’, but he none the less acknowl-edged that it was fundamentally reshaping society ... copies of the productions of genius and compilations of learning, bringingthem within the reach of all ranks of men’.20Exchanges in printmight lead to new ideas, but literature’s role as...
... teaching. The findings ofthe study demarcate that the teachers were following both the traditional and CLT approach in their practice.While addressing the issues regarding the Indonesian ministry ... away.That is genuine communication incorporating an information gap. The concept of information gap seems to be one ofthe most fundamental inthe whole area of communicative teaching. Any exercise ... participants inthe language learning process. As a result, CLT also alters theroleofthe teacher. According to Breen and Candlin (1980), " ;the teacher has two main roles: the first role is...
... data sets, the results for 5HW inthe N-domain of TnC, inthe inhibitory region of TnI and at position 121 of TnI, followed the Ca2+-binding to the N-domain of TnC. The variation in Kdand ... Ca2+signal is related to the N-domain. Further, the disruption ofthe sites inthe C-domain affects the loweraffinity part ofthe signal. The difference inthe second part of the curve, however, is ... seems to havemainly a structural function in maintaining the organization of the Tn [7–9,45]. The function ofthe C-terminal region of TnI is less understood. Mapping ofthe TnI interactionsFig....
... simulation of DK9thrombin in explicit water solvent supports theroleof the A-chain in affecting the conformation and catalyticproperties ofthe B-chain, particularly in some insertionloops ofthe ... thrombin show that the A-chain closely follows the contour ofthe catalyticB-chain, hinging the two interacting six-stranded bar-rel-like domains ofthe B-chain [17]. A well-structurednetwork of ... single band of ~ 36 kDa for all the thrombin forms.Effect of pH on thrombin amidase activity andbinding ofthe inhibitor a-NAPAP to the thrombinactive site The effects of pH (5.5–10) on the...
... level of development, the prevalence of obesity (and of type 2 diabetes) is linked to the degree of inequality insociety (measured us-ing such indicators as the Gini coefficient), rather than the ... education ofthe mother and watching more television, explained most ofthe difference inthe prevalence of obesity by ethnic origin. Finally, other risk factors, such as tobacco smoking and ... processes directly and indirectly in uencing the prevalence of obesity THE DETERMINANTS ã 132. The determinants of obesityMain messagesã The simultaneous presence of physical inactivity and poor...
... confirm the expression of proseminmRNA in some cell lines. A band ofthe predicted size(500 bp) ofthe PCR product was observed for most of the cell lines tested, including HPC-YO and HPC-YS, in ... Black bars indicate the enzymatic activity of entero-kinase on the substrate. The closed circles and line indicate prosemin enzyme activity after activation ofthe protein by enterokinase; the enzymatic ... indicating successfuldigestion ofthe artificial trypsinogen pro-enzyme frag-ment. The amount of 31 kDa prosemin increased in pro-portion to the time of incubation with enterokinase.Prosemin activity...
... inthe case ofthe K137E mutant of HSP22 (Fig. 4B); however, in this case, the intensity of the band corresponding to the HSP22 dimer wasmore intense than inthe case ofthe wild-type protein.Thus, ... intact protein at the beginning of tryp-sinolysis and at the fixed time of trypsinolysis) against the time of incubation (Fig. 8D). The apparent rateconstants of trypsinolysis under these conditions ... shock proteins, each of which contains the so-called a-crystallin domain consisting of 80–100 residues and located inthe C-terminal part ofthe molecule. The a-crystallin domain consists of six...
... is critical in providing proper docking sites, either directly or indirectly,by modifying the conformation of PSII on the lumenal side,making efficient binding of PsbQ and PsbP ofthe OECpossible.A ... emphasizing the roleof these two subunits as primary assembly partnersfor construction ofthe PSII complexes. Indeed, the PsbEprotein was also present in tiny amounts inthe thylakoidmembranes of ... R.&Yamamoto,Y.(2003)Dynamicinteractionbetween the D1 protein, CP43 and OEC33 at the lumenal side of photosystem II in spinach chloroplasts: evidence from light-induced cross-linking ofthe proteins inthe donor-side...
... demonstration of BMPs 2. To examine the reactions of farmers to the BMP demonstration trials in light of these issues, and 3. To assess theroleof farmer associations (aqua clubs) in facilitating the ... four major catfish farming provinces inthe Mekong Delta, but also included surveys of other BMP farmers and some non BMP farmers inthe other major provinces ofthe Delta. The fieldwork component ... multiple business enterprises, including acting as a market chain middleman, and has a fish feeding business. These examples highlight not only the complexity and diversity of farmers inthe industry...
... wellsanddistancetoroads;Table2).Wealsoretainedthedate of initiation ofthe incubation process (Julian date)and the nest age inthe model (Table 2). The final land-scape model thus included seven covariates, including the intercept.We ... wind direction to navigateto the source ofthe odor (i.e., the nesting bird). It isinteresting to note that the two weather variables mostresponsible for olfaction were included into the finalmodels ... of female greater sage-grouse and nests, and corresponding nest fates, on the 5,625-km2study area inthe Wind River Basin in centralWyoming, USA.bDates listed are for the initiation of the...
... previous data showing that renal injury in the MRL mouse is independent of Fc receptors [51], our stud-ies showing the functional deficiency of C4 in these mice, andthat the loss ofthe major negative ... C4b-binding protein deficiency. Blood1990, 76:2527-2529.14. Hessing M, van TVC, Bouma BN: The binding site of humanC4b-binding protein on complement C4 is localized in the alpha'-chain. ... seven α chains cova-lently bound to each other and to one β chain. Other isoformscontain either seven α chains without a β chain or six α chainswith one β chain. The α chain is composed of eight...