ethical evaluation of translational stem cell research projects in research ethics committees
Asymmetric cell division in the regulation of neural stem cell self renewel in drosophila melanogaster
... germline stem cell and its surrounding cap cells (in ovary) or hub cells (in testes) is a good example of how stem cells utilizing this mechanism establishes polarity and maintains its stem cell ... function in promoting and inhibiting stem cell self-renewal, loss of Pins function causes a reduction in neuroblast numbers in the larval brain due to occurrence of GMC/GMC siblings and in turn ... results in two Introduction daughter cells that are distinct at birth The neural stem cells in the developing central nervous system (CNS) of Drosophila adopt this intrinsic regulation of asymmetric...
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university of california press fundamentals of the stem cell debate the scientific religious ethical and political issues dec 2007
... the same cells and in different cells (D) These cells, grown from the neural retina, show staining common to brain neural stem cells (DCX, red) and staining found only in neural stem cells derived ... Doublecortin (DCX, red), vimentin (green), and nestin (blue) staining in a neurosphere demonstrate the intimate commingling of the cells in a sphere as well as expression of multiple markers both in ... determinants during division of a neural stem cell in a fly embryo, showing the opposite localizations for ACD determinants in the stem cell and differentiating cell The Miranda protein, stained red,...
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báo cáo khoa học: "The research on the immuno-modulatory defect of Mesenchymal Stem Cell from Chronic Myeloid Leukemia patients" pdf
... differentiate into malignant blood cells and phenotypically defined endothelial cells at the single -cell level, suggesting these cells have the properties of hemangioblasts Page of 10 The main purpose of ... (ng/ml) in the culture supernatants of CML snc-RNA (20 nM) or ds-RNA (1-20 nM) cells were determined by enzymelinked immunosorbent assays Intimab(a BCR/ABL tyrosine kinase inhibitor) and stem cell ... from the “863 Projects of Ministry of Science and Technology of PR China (No 2006AA02A109 2006AA02A115); National Natural Science Foundation of China (No.30570771; Beijing Ministry of Science and...
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Guidelines for Human Embryonic Stem Cell Research potx
... Banking of hES Cell Lines As hES cell research advances, it will be increasingly important for institutions that obtain, store, and use cell lines to have confidence in the value of stored cells, ... for federal funding of research deriving and using hES cells from multiple sources, including NT, asserting that, without government funding of basic research concerning stem cells, progress ... descriptive information on the types of hES cell research in which they are engaged The purposes of such a registry include facilitating distribution of educational information in light of evolving ethical, ...
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báo cáo hóa học: " A psychometric evaluation of the PedsQL™ Family Impact Module in parents of children with sickle cell disease" pptx
... work in determining disease severity [12-16] All others were classified as having mild disease The Institutional Review Board of the Children's Hospital of Wisconsin/Medical College of Wisconsin ... Lastly, to aid in determining discriminant validity of the PedsQL™ Family Impact Module in our population of children with sickle cell disease, we collected data on the disease status of the children ... sample of parents of children with and without sickle cell disease at both clinics as part of a larger study of the HRQL of children [13,25] There were 145 parents of children with sickle cell...
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Báo cáo hóa học: " Research Article Performance Evaluation of Uplink Delay-Tolerant Packet Service in IEEE 802.16-Based Networks" potx
... schemes in IEEE 802.16 In Section 3, we provide the analytical model including the details of the capacity allocation and the uplink scheduling The analysis of the queueing model follows in Section ... how to take into account an upper bound on mean delay of the nrtPS service flow at the SSs in determining the maximum of the sum of the real-time capacities at every SS Finally, we introduce a ... Networking Frame Downlink (DL) subframe Uplink (UL) subframe UL-MAP indicates the starting time slot of each uplink burst Reservation interval (RI) UL-MAP DL-MAP Preamble SS1 transmission interval...
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Báo cáo hóa học: " Research Article Evaluation of Cross-Layer Rate-Aware Routing in a Wireless Mesh Network Test Bed" pdf
... chose to perform measurements using TCP traffic instead of UDP traffic because it gives a better idea of the kind of connection available Indeed, most of the traffic in the Internet is TCP, furthermore, ... laptops running any modern operating system.2 Usual Internet usage including basic services like web browsing, e-mailing, ssh, and so (2) where Ri, j is the raw data transmission rate on the link from ... task, reducing the overhead introduced on the network in order to maintain active all connections of clients moving and associating to different MeshDVBoxes Hereafter we give an overview of the MeshDV...
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ADVANCES IN HEMATOPOIETIC STEM CELL RESEARCH pot
... stimuli trigger intrinsic determinants of cell fate, the transcription factors which contribute to the reprogramming of HSCs into cell- Advances in Hematopoietic Stem Cell Research lineage restricted ... proteins are required for the maintenance of embryonic as well as a broad range of adult stem cells, including hematopoietic stem cells (HSCs) PcG proteins maintain the self-renewal capacity of ... role in all types of stem cells In some stem cells there is an intrinsic polarity where molecules are segregated along an axis and serves as determinant of cell fate after cell division In these...
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Báo cáo sinh học: "Q&A: What can microfluidics do for stem-cell research" ppsx
... forces applied to individual cells determine their behavior What major problems in translational stem cell biology can be addressed using microfluidics tools? Here again, microfluidics techniques ... growth and protection of crystals [3] For cell biologists, the major impact has been in cell separation, single -cell resolution of the dynamics of gene expression, and insights into how mechanical ... manipulated indepen dently, making it possible to determine the hierarchy of stimuli that determine cell behavior An obvious advantage of microfluidics is that it provides economy in terms of reagent...
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Báo cáo sinh học: "Acoel and platyhelminth models for stem-cell research" pptx
... However, current research in stem- cell biology is providing increasing evidence that the behaviors and fates of stem cells are not inherent, cell- autonomous properties, but are instead critically ... attractive invertebrate models for stem- cell research They present a number of advantages, including the fact that a large pool of stem cells is available throughout the lifetime of each individual; ... recent progeny of neoblasts express a sequence of distinct transcriptional profiles as they migrate and begin to differentiate [7] Interestingly, in that study, several chromatin-modifying factors...
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Báo cáo y học: "proteomic characterization of non-small cell lung cancer in a comprehensive translational thoracic oncology database" docx
... al Journal of Clinical Bioinformatics 2011, 1:8 http://www.jclinbioinformatics.com/content/1/1/8 staining compares the relative intensity of staining of one core of a TMA to that of a control ... score of indicates faint staining, indicates medium intensity staining, and indicates dark staining Furthermore, the pathologist is also able to visually assess the localization of predominant ... serving as a proxy for higher protein expression Two measures, the percent and intensity of IHC staining, were used to describe the level of protein expression in a tissue sample Percent staining...
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báo cáo khoa học: "Current status of haploidentical stem cell transplantation for leukemia" pps
... "megadose" of donor hematopoietic stem cells by combining G-CSF mobilized peripheral blood with bone marrow stem cells, both depleted of T cells, following TBI-based conditioning regimens[16-18] In their ... obstacles of mismatched HSCT in leukemia was to increase stem cell numbers in graft With the pioneering work of Reisner[15], Aversa and his group produced a series of clinical trials, administered ... reflection of the heterogeneity of patients being treated And due to the high incidence and severity of GVHD, the safest approach of DLI after haploidentical HSCT, including cell dose, time of infusion,...
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Báo cáo y học: ":Identification of novel stem cell markers using gap analysis of gene expression data" ppsx
... their expression Rab family of GTPases: Rab3d The Rab family of small GTPases are involved in intracellular cell signaling processes, including tethering and docking of vesicles to their target ... usefulness of the selected patterns within the context of stem cell research By examining the literature, we selected 88 marker genes that represented the variety of stem cell types in our dataset ... set of 12 genes, five of which belonged to the family of serpins (SERine Protease INhibitors; serpins a1b, a3n, b9, g1, and h1) Serpins are a large class of proteins that are found in all multicellular...
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Báo cáo y học: "A full menu for stem-cell research" pps
... therapeutically relevant Cancer stem cells Self-renewal is the hallmark of both stem cells and cancer cells, and in his talk on leukemia stem cells, Weissman addressed this intriguing parallel Many pathways ... suggested that stem- cell research will lead to insights in a variety of fields, such as embryology, cell- based therapy and the origin of cancer We look forward to seeing advances in the field, ... identification in his laboratory of alternative resident adult stem- cell populations in skeletal muscle His group found that within the satellite -cell compartment in human muscle, 1% of the cells also...
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Development of human stem cell based model for developmental toxicity testing
... Specific inhibition of integrin α6β1-laminin binding attenuated cell- ECM interaction and resulted in contraction of μP-hPSC colonies (a) Phase images of μP-hPSC colonies after 24 hr of mesoendoderm ... localization of integrin mediated cell- matrix adhesion in the μP-hPSC colony Images are immunofluorescence projections of 3D confocal sections of integrin β1, vinculin and paxillin before (0 ... Figure 9.3.2 Blocking of laminin- α6β1 integrin binding by α6β1 antibody caused contraction of differentiating μP-hPSC colonies in a dosedependent manner (a) Phase images showing μP-hPSC colonies...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 2
... recombinant Lefty1 protein was able to block reduction in level of alkaline phosphatase staining induced by Lefty2 RNAi Alkalline phosphatase staining was carried out day post transfection of Lefty2 ... effect in maintaining the undifferentiated cell state (Figure 2) One interesting factor identified from the screen is Lefty2, a secreted protein well known for its role in regulating Nodal signaling ... maintain ES cell self renewal was investigated in mouse ES cells Lefty is known to be a secreted protein We hence wanted to first confirm that its site of action is extracellular in the ES cell...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 3
... signals dictate embryonic stem cell fates is also not entirely clear Conflicting roles of Nodal signaling in both the maintenance of ES cell self renewal and induction of ES cell differentiation have ... Nodal signaling might, as in the in vivo scenario, likewise influence ES cell fate decision in a dose dependent manner in vitro is addressed Interestingly, it was found that Nodal signaling is able ... addition of Activin A ligand causes differentiation of ES cells into meso/mesendodermal lineages while repression of signaling with the specific chemical inhibitor SB431542 and Lefty protein supplementation...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 4
... duration of the experiment The E14 cells were fed daily with medium containing fresh Activin A 4.14.2 Inhibition of Nodal/Activin signaling To abolish/diminish Nodal/Activin signaling, the cells ... the C-terminus of Lefty2 was chosen instead of the N-terminus because tagging at N-terminus may result in the V5 tag being cleaved off during LEFTY2 processing and secretion The insert was amplified ... day of selection 4.9 Alkaline phosphatase staining Detection of alkaline phosphatase, which is indicative of the nondifferentiated state of ES cells, was carried out using a commercial ES Cell...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 5
... M T., King, C C., and Hayek, A (2005) Activin A maintains pluripotency of human embryonic stem cells in the absence of feeder layers Stem Cells 23, 489-495 Besser, D (2004) Expression of nodal, ... expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells Cell 113, 643-655 Chambers, I., and Smith, A (2004) Self-renewal of teratocarcinoma and embryonic stem cells Oncogene ... Transcriptome profiling of human and murine ESCs identifies divergent paths required to maintain the stem cell state Stem Cells 23, 166-185 Wieduwilt Matthew J., (2003) SMAD3 in embryonic patterning, mesoderm...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency
... pluripotent embryonic stem (ES) cell lines The derivation of pluripotent cell lines from blastocysts was first achieved in the murine system in 1981 by Martin Evans and Matthew Kaufman and independently ... types of the three germ layers in culture Another defining feature of ES cells is their acquired capacity to proliferate indefinitely in vitro without undergoing senescence while retaining a ... understanding of the molecular mechanism underlying self renewal and cell fate determination of ES cells 1.2 Molecular basis underlying ES cell self renewal Despite the importance of stem cell self...
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