establishment of new lines of human embryonic stem cells evolution of the methodology
Establishment of autologous culture systems for human embryonic stem cells
... Characterization of stem cells by derivation source Category Embryonic stem cells Embryonic germ cells Fetal stem cells Umbilical cord stem cells Umbilical cord blood stem cells Umbilical cord matrix stem cells ... to their derivation sources, named embryonic stem cells Literature review (ESCs), embryonic germ cells, fetal stem cells, umbilical cord stem cells, adult stem cells and induced pluripotent stem ... while others have also demonstrated an alternative of culturing hESCs on a feeder-free system with the aid of MatrigelTM But none of them has systematically compared the supportive abilities of these...
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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
... of ATP in the dynamics of the actin filaments of the cytoskeleton Biol Chem 2006;387(4):401-6 Lopez LA, Bertini F The in vivo effect of low body temperatures on the degree of polymerization of ... that these cells were also highly sensitive to low temperature exposure, just like the embryos from which they originated Hence, the first few reported studies [7, 8] on the establishment of new ... new hESC lines emphasized the use of temperature-heated stage and pre-warmed and pre-equilibrated culture media Nevertheless, the results of this study would prove otherwise hESC of the H9 line...
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báo cáo hóa học:" Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice" docx
... vascular cells on the CBF in the ipsilateral side Effects of the transplanted vascular cells on the CBF in the ipsilateral side A-C: LDPI analysis of the CBF by LDPI evaluated in mice with the scalp ... quantifying the ratio of the pixels of human and/or mouse PECAM-1-positive cells to 512 × 512 pixels in that field: the ratio was expressed as %area The number of transplanted MCs detected in the ischemic ... and MCs derived from human ES cells (hESECs+MCs) The cell mixture was composed of almost the same number of ECs and MCs We resorted the VE-cadherin+ cells from these expanded cells to obtain ECs...
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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
... are names of human embryonic stem (hES) cells lines P denoted the number of passages of the cell lines H9-EB denoted embryoid body (EB) prepared from cell line H9 and the day indicates the time ... names of hES cells lines P denotes the number of passages of the cell lines H9-EB denotes embryoid body (EB) prepared from cell line H9 and the day indicates the time in culture HMVEC = human ... development of immune system [15] Signature miRNAs, such as the miR-302 family, the miR-200 family have been reported in human [16,17] and mouse embryonic stem cells [18-20] The unique patterns of miRNA...
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báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx
... embryonic stem cell; hESC, human embryonic stem cell; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of ... epigenomic profiling of a cohort of human embryonic stem cell (hESC) lines can be used to generate hESC reference epigenomes (left panels) The extent of reprogramming and differentiation potential of individual ... the functional regulatory space, or ‘regulatome’, of a given cell type and to study the significance of the non-coding genetic variation in human disease (a) The vast non-coding fraction of the...
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Differentiation and derivation of lineage committed chondroprogenitors and chondrogenic cells from human embryonic stem cells for cartilage tissue engineering and regeneration
... characterized by the breakdown of the joint’s cartilage Cartilage is the part of the joint that cushions the ends of the bones and allows easy movement of joints The breakdown of cartilage causes the bones ... functionality of ESCs 2.2 Human Embryonic Stem Cells (hESCs) Embryonic stem cells (ESCs) are derived from the inner cell mass of the blastocyst-stage embryos and are characterized by their capacity ... model system set up to study the effect of growth factors on chondrogenic differentiation of hESCs would be useful for the dissection of the role of growth factors and their signaling in embryonic...
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Role of sonic hedgehog signalling in human embryonic stem cells and its neural derivatives
... 2006) 2.7 Embryonic stem cells and induced pluripotent stem cells Embryonic stem cells (ESC) have two characteristics that distinguish them from other stem cells, in that they are capable of long-term ... 161! vii SUMMARY Human embryonic stem cells (hESC) are pluripotent stem cells that have the unique ability to differentiate into cells of the three germ line lineages Hence, they have wide potential ... understanding of the role of SHH in humans, that is based largely on the mouse model Figure 2.6 Human embryonic stem cells (hESC) derived from the blastocyst are able to differentiate into cells from...
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Baculovirus mediated genetic modification of human embryonic stem cells
... characterized the integration sites of the transgene expression cassette VII Finally, the effects of the baculoviral transduction of the human embryonic stem cells were investigated The transduction of human ... with new functional properties to combat specific diseases in the course of ex vivo gene therapy 1.1.1 Human Embryonic Stem Cells Human embryonic stem (hES) cells are cells derived from the inner ... MODIFICATION OF HUMAN EMBRYONIC STEM CELLS DU JUAN (B Sc.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF BIOLOGICAL SCIENCES NATIONAL UNIVERSITY OF SINGAPORE AND INSTITUTE OF...
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The derivation, propagation, storage and gene expression of human embryonic stem cells on human feeders
... classes of pluripotent stem cells in humans, other primates and mice These are embryonic stem cells, embryonic germ cells, embryonic carcinoma cells and recently the discovery of a fourth class of ... entails the delivery of stem cells to the tissues in which they are needed Current practice involves either the injection of stem cells directly into the targeted tissue, or injection of the stem cells ... cells include the bulk of the cells that make up the human adult and each of these cells in their differentiated state has its own copy or copies of the genome with the only exception being cells...
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Directed differentiation of human embryonic stem cells into haematopoietic and definitive endodermal lineages
... either side of the primitive streak (Tam and Behringer 1997) Extensive studies on cells of the cardiac mesoderm showed that the timing of ingression through the streak and the position of these ... events was made possible with the isolation of embryonic stem cells Extensive investigation of the properties and capabilities of these cells has led to the development of several experimental approaches ... Working with the simple goal of replacing defective, diseased or lost cell types, embryonic stem cell-based therapy promised the repair of damaged tissue/s or organs of the human body ES cells are...
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IN VIVO EX VIVO OSTEOGENESIS OF HUMAN EMBRYONIC STEM CELLS
... cells is mandatory 1.5 Human Embryonic stem cells As the name suggests, the human embryonic stem cells are derived from the early embryo These cells have the capacity to self renew and can be directed ... Although, these cells have a high capacity to self renew in culture, their ability to expand is less than that for embryonic stem cells The self renewal and the proliferative capacity of these stem cells ... from the bone marrow (mesenchymal stem cells, hematopoietic stem cells) and (2) embryonic stem and embryonic germ cells derived from discarded human embryos and germ line stem cells Adult stem cells...
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Transcriptome study of human embryonic stem cells and knockdown study of a pluripotency marker, LIN28
... Introduction 1.1 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells Embryonic stem (ES) cells are isolated from the inner cell mass (ICM) of embryos of blastocyst ... indicated the uniqueness of the human ES cells from the rest of the cells, cancerous or normal and that human EC cells shared a common core set of pluripotency genes with human ES cells In the ES/EC ... HES3 Human Embryonic Stem Cells Undifferentiated HES4 Human Embryonic Stem Cells SAGE _Embryonic_ stem_ cell_H9_normal_p38_CL_SHES1 LSAGE _Embryonic_ stem_ cell_BG01_normal_p20_CL_SHE19 LSAGE _Embryonic_ stem_ cell_H13_normal_p22_CL_SHE15...
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báo cáo hóa học:" Human embryonic stem cells hemangioblast express HLA-antigens" pot
... examined the expression profile of HLA molecules on the surface of human ES cells, EB cells and blast-like cells We demonstrated extremely low levels of HLA-A2 expression in the undifferentiated H9 human ... blast cells The bipotentiality of hemangioblast and blast cells provides the opportunity to use them in future cellular therapies of human disorders Moreover, the blast cells can possibly find their ... however, that the expression levels of HLA-A2 proteins on the surface of human ES-derived blast cells were still lower than those observed in the control human somatic cells This lower level of HLA-A2...
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Báo cáo khoa học: "Human embryonic stem cells and therapeutic cloning" pdf
... Culture and characterization of human embryonic stem cells Stem Cells Dev 2004, 13, 325-336 18 Drukker M, Benvenisty N The immunogenicity of human embryonic stem- derived cells Trends Biotechnol 2004, ... numbers of ES cells can be relatively easily obtained in vitro culture Therefore, ES cells could be the choice of cells in stem cell therapy for various diseases One of the critical steps for stem ... oviductal fluid (SOF) with amino acids (hmSOFaa) by supplementing mSOFaa with human serum Establishment and characterization of human cloned ES cells Having thoroughly the proved concept of therapeutic...
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Human embryonic stem cells as a cellular model for osteogenesis in implant testing and drug discovery
... three types of stem cells, adult stem cells, embryonic stem cells and induced pluripotent stem cells, embryonic stem cells are inevitable and irreplaceable source to be studied Hence, for the projects ... discussed above, the problems we face today, may severely limit the use of adult stem cells either in research or clinical applications 1.2.2.2 Embryonic stem cells: Another type of stem cells by conventional ... Material of implants 1.1.2 Biocompatibility 1.2 Stem cells ………………………………………………………………….4 1.2.1 Significance in the use of stem cells 1.2.2 Definition of stem cells 1.2.2.1 Adult stem cells 1.2.2.2 Embryonic...
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Human embryonic stem cells for genotoxicity testing
... However, established cell lines poorly reflect human physiology Lack of standardization is also a limitation with the use of primary explanted cells Human embryonic stem cells (hESCs) have been ... Determination of Genotoxicity using Mitomycin C…………………………… 26 iii CHAPTER 4 RESULTS……………………………………………………… 27 4.1 Characterization of human embryonic stem cells …………………… 27 4.2 Temperature tolerance of human ... evidenced by the lower levels of chromosomal aberration in hESCs compared to IMR-90 The resistance of hESCs to genotoxic damage highlights the possibility of efficient repair mechanisms in these cells...
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Báo cáo y học: "A comparative analysis of DNA methylation across human embryonic stem cell lines" docx
... pluripotent stem cells However, less is known about the conservation and variability of DNA methylation across different stem cell lines A recent analysis of about 1% of the genome of HESC lines shows ... unconserved The number of cytosines in the groups is compared to a null model that assumes the independence of methylation between the two cell lines Thus, the more significant the deviation between the ... with the changes of the expression levels of their associated genes The coordinates of TFBSs were downloaded from Motifmap [39] (sites with FDR < 0.1) We determined the methylation level of these...
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Báo cáo y học: " Derivation of normal macrophages from human embryonic stem (hES) cells for applications in HIV gene therapy" pps
... derivation of human embryonic stem cells Stem cells 2005, 23:1228-1233 Moon SY, Park YB, Kim D-S, Oh SK, Kim D-W: Generation, culture, and differentiation of human embryonic stem cells for therapeutic ... differentiation ofhuman embryonic stem cells Blood 2003, 102:906-915 Wang L, Li L, Menendez P, Cerdan C, Bhatia M: Human embryonic stem cells maintained in the absence of mouse embryonic fibroblasts ... Background Human embryonic stem cells (hES cells) show great promise for many novel cellular therapies due to their pluripotent nature [1] These cells have the capacity to give rise to mature cells...
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Báo cáo y học: "Identification and isolation of embryonic stem cells in reproductive endocrinology: theoretical protocols for conservation of human embryos derived from in vitro fertilization" ppsx
... presents a substantial ethical obstacle for the advancement of human ESC research With the vast therapeutic promise of human ESC seen against the destruction of human embryos required to realize such ... obtained for further analysis Refinement and increased efficiency of these two ESC protocols brings the potential to offer a reliable supply of embryonic stem cells without production of whole embryos ... 114:1364-1370 Donovan PJ, Gearhart J: The end of the beginning for pluripotent stem cells Nature 2001, 414:92-97 23 24 25 26 Doerflinger RM: The ethics of funding embryonic stem cell research: a Catholic...
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Báo cáo y học: " GeneChip analysis of human embryonic stem cell differentiation into hemangioblasts: an in silico dissection of mixed phenotypes" docx
... 2007, Background The establishment of human embryonic stem cells (hESCs) raised the possibility of being able to treat/cure many human diseases that are nowadays untreatable This therapeutic potential, ... al.: Genome wide profiling of human embryonic stem cells (hESCs), their derivatives and embryonal carcinoma cells to develop base profiles of U.S Federal government approved hESC lines BMC Dev Biol ... derived from human embryonic stem cells Proc Natl Acad Sci USA 2002, 99:4391-4396 Choi K: The hemangioblast: a common progenitor of hematopoietic and endothelial cells J Hematother Stem Cell Res...
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