embryonic stem cells as a source of differentiated neural cells for pharmacological screens pdf
báo cáo hóa học: " The microglial NADPH oxidase complex as a source of oxidative stress in Alzheimer''''s disease" ppt
... Shimohama S, Tanino H, Kawakami N, Okamura N, Kodama H, Yamaguchi T, Hayakawa T, Nunomura A, Chiba S, Perry G, Smith MA, Fujimoto S: Activation of NADPH oxidase in Alzheimer's disease brains Biochem ... microglial NADPH oxidase maybe largely responsible for the oxidative damage observed in the AD brain Astrocytes and the NADPH oxidase Astrocytes are the most abundant glial cell type in the brain and ... complex and the initiation of intracellular signaling events regulating oxidase assembly and activation has been described [31,32] The NADPH oxidase The phagocytic NADPH oxidase plays an essential...
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examining linguistic ambiguity as a source of constructing funniness in english verbal jockes = khảo sát hiện tượng mơ hồ ngôn ngữ với vai trò là một nguồn tạo nên tính hài hước của các câu chuyện tếu tiếng anh
... conveying at least two incompatible interpretations- “having more than one sense” as stated by Hurford and Heasley (2001:121) -9- Regarding paraphrasing, Hurford and Heasley (2001) assert that a word ... chapter in fact is a typical example of this (2) A man eating a kebab goes up to a lady who has a yapping Chihuahua at her heels “Can I throw your dog a bit?” he asked politely “Certainly,” came ... considered ambiguous if and only if it has at least two paraphrases that are not themselves paraphrases of one another A good case in point can be found in: (3) The chicken is ready to eat It is...
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Differentiation and derivation of lineage committed chondroprogenitors and chondrogenic cells from human embryonic stem cells for cartilage tissue engineering and regeneration
... LIST OF ABBREVIATIONS Alkaline phosphatase - ALP Alcian blue - AB Alpha fetoprotein - AFP / αFP Autologous chondrocyte implantation - ACI Ascorbic acid 2-phosphate - AA2P β-actin - BA Bone marrow ... 2.1 Articular cartilage and its associated clinical problems Articular cartilage is a unique avascular, aneural and alymphatic load-bearing tissue which is supported by the underlying subchondral ... with appearance of adult collagen isoform, Col IIB, as early as day of EB formation (Kim et al., 200 5a) In contrast, normal wild type 5-day (5‘d’) EBs only displayed Col IIB after 14 days of chondrogenic...
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Food as a Source of Dioxin Exposure in the Residents of Bien Hoa City, Vietnam
... Channa Striata—snakehead Anabas Testudineus— climbing perch Clarias Fuscus— catfish Clarias Fuscus— catfish Ostechilus Hasselti— carp Discussion This is the most recent VietnamU.S collaborative ... 3.3 40 74 2.9 0.95 68 Channa Striata—snakehead Anabas Testudineus— climbing perch Clarias Fuscus— catfish Clarias Fuscus— catfish Ostechilus Hasselti— carp TABLE Comparison of Highest Dioxin TEQ ... Channa Striata—snakehead Fish 2: Anabas Testudineus— climbing perch Fish 3: Clarias Fuscus— catfish Fish 4: Clarias Fuscus— catfish Fish 5: Ostechilus Hasselti— carp ND—nondetected, limit of...
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Báo cáo khoa học: "Human embryonic stem cells and therapeutic cloning" pdf
... clinical trails that employ adult stem cells (such as blood-forming hematopoietic stem cells and cartilage-forming cells) A potential advantage of using adult stem cells is that the patient's own cells ... DNAspindle complex is extruded through a small hole in the zona pellucida, instead of aspirating the DNA-spindle complex with a glass pipette as others have described [81] With use of an aspiration ... Miyamoto K, Hayashi K, Suzuki T, Ichihara S, Yamada T, Kano Y, Yamabe T, Ito Y Human placenta feeder layers support undifferentiated growth of primate embryonic stem cells Stem Cells 2004, 22,...
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Human embryonic stem cells for genotoxicity testing
... 11 Karyotype of hESCs maintained at oC for 24h by mFISH 37 Figure 12 Metaphase spread of hESCs maintained at 25 oC for 24h by mFISH 38 Figure 13 Metaphase spread of hESCs maintained at oC for ... 14 Metaphase spread of hESCs maintained at oC for 48h by mFISH 39 Figure 15 Karyotype of hESCs maintained at oC for 48h by mFISH 39 Figure 16 Metaphase spread of hESCs maintained at 25 oC for 48h ... Established mammalian cell lines and primary explanted cells from mammals are commonly used in vitro to analyze the genotoxic potential of environmental factors, drugs, biomaterials as well as chemical,...
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Báo cáo y học: "Identification of a truncated form of methionine sulfoxide reductase a expressed in mouse embryonic stem cells" pps
... collection and assembly of data, data analysis and interpretation, manuscript writing and final approval of manuscript for publication YJ Provided study materials, collection of data, data analysis and ... pairs to amplify the truncated form of MsrA and introduce a BamHI cutting site at the 3’ end (MsrA -for: 5’-cctggctgcggaggtggagaaac and MsrA-BamHI-rev: 5’-tggggccaaggatccgctttgaaagaacc) The amplicons ... data, data analysis and interpretation, manuscript writing and final approval of the manuscript XH Participated in the collection and assembly of data, data analysis and interpretation, manuscript...
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Báo cáo y học: "A recessive genetic screen for host factors required for retroviral infection in a library of insertionally mutated Blm-deficient embryonic stem cells" doc
... HMSpBb-Sau3AI 5'-gat cCC ACT AGT GTC GAC ACC AGT CTC TAA (T)10C (A) 7-3' HMSpBb-FatI 5'-cat gCC ACT AGT GTC GAC ACC AGT CTC TAA (T)10C (A) 7-3' Splinkerette PCR primers AB949new 5'-GCT AGC TTG CCA AAC ... 5'-CGA TGA TGT AGG AGA GAA TCA GGT-3' mCAT1-exon4-F 5'-GTA CTT CAA GCG TGG CAA GAG-3' mCAT1-exon7-R 5'-CTG GTG GAA AGT GCG CAG AGA-3' mCAT1-exon8-F 5'-TCT CCT AGG CTC CAT GTT TCC C-3' mCAT1-exon12-R ... 5'-CTA TCA GCA TCC ACA CTG CAA A- 3' LacZ-Gsp2-R 5'-ATG TGC TGC AAG GCG ATT AAG-3' Genomic PCR primers Actb-exon4-up 5'-GTT TGA GAC CTT CAA CAC CCC-3' Actb-exon4-down 5'-GTG GCC ATC TCC TGC TCG AAG...
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Transcriptome study of human embryonic stem cells and knockdown study of a pluripotency marker, LIN28
... GTTGTTACCTCAAACCTCCTTTCC GCACCACGAACGCCTTTG GCGGTGTGCGGATGGTA CCCTAGAGATAAGGCGCTTCAG AAGATGGTGGATGCTTCCAAAA ACCACTCGGAGGACCTGTTTT ACAGCAAATGACAGCTGCAAA ACCTGCTGGGCCCACAT CAACATGAACGGACAGCTCAAC GACGGTCCATGCTCAATGGT ... CGCGTCATCTGTAAGTGGTTCAACGTTTCAAGAGAACGTTGAACCAC TTACAGATGTTTTTCATATGAT 3’ LIN28sh2R 5’ CGATCATATGAAAAACATCTGTAAGTGGTTCAACGTTCTCTTGAAAC GTTGAACCACTTACAGATGA 3’ Page 24 NM_024674.4 2.4 Transfection and ... TGGGCATCAGGCCAAGTC TGCAGGTCCCTTGGACATG TGGCGCCGGTTACAGAAC AAGCTGTATATTTACTCATTGAAA CAC GCCATCATCATTACCCATTGC GCCCAATACGACCAAATCC ACCCGTGGTCACCATGGTA Chapter Results 3.1 SAGE data analysis to search...
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Human embryonic stem cells as a cellular model for osteogenesis in implant testing and drug discovery
... OCT4 F: CGRGAAGCTGGAGGAGAAGGAGAAGCTG 55 oC R: AAGGGCCGCAGCTTACACATGTTC NANOG F: GGCAAACAACCCACTTCTGC 55 oC R: TGTTCCAGGCCTGATTGTTC β-ACTIN F: ACAGAGCCTCGCCTTTGCC 58 oC R: ACATGCCGGAGCCGTTGTC 2.1.5 ... the standard of using hESCs as a model In this study, taking hESCs differentiation towards osteogenic lineage as an example, we proposed a set of functional assays as a standard of hESCs as a model ... lines for hours following ISO 10993 standards DMSO was used as control MTS assay is a standard laboratory colorimetric assay that measures the activity of mitochondrial activity Enzyme reductase...
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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
... weak expression of SSEA3 and TRA-1-81 A total of 200 colonies were examined for each experimental group, as well as for the control Statistical comparison of data was performed by the Chi-squared ... clumps for serial passage was achieved through treatment with mg/ml collagenase type IV, for between to Exposure of hESC to reduced temperature After days of culture following the last serial passage, ... passages Their appearance was virtually indistinguishable from non-temperature exposed hESC (data not shown) Chromosomal Analysis of hESC after exposure to low temperature Metaphase spreads of...
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Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx
... 5¢-TTCCTACACATTAACGAGCCTCTGC-3¢ and DS 5¢AACATCTGGCCACACACCCTAAGC-3¢; ⁄ 2flank, US 5¢-CTATTTGCTAACAGTCTGACAATAGAGTAG-3¢ and DS 5¢-GTTACATATGCAGCTAAAGCCACAAATC-3¢; mouse Rex-1: US 5¢AACTGCATCCTCTGCTTGTG-3¢ ... 5¢CACCTAAGACACTGTG GAAGAGCAG-3¢; mouseHS2 US: 5¢GGGTCTCTCTA GGAGGAAGTCCACAGG-3¢ and DS: 5¢CAGATCTAAT GACCCTAACTCTAAC-3¢; mouse bmajor US: 5¢GGT GCACCTGACTGATGCTGAGAAG-3¢and DS: 5¢GTG GTACTTGTGAGCCAGGGCAGTG3¢ ... 5¢-CCACCAGCTATCA GGGCCCAG-3¢ and DS, 5¢-GCTGCTATGCTGTGCCTC3¢; human5¢HS2: US, 5¢-TGGGGACTCGAAAATCAA AG-3¢ and DS, 5¢-AGTAAGAAGCAAGGGCCACA-3¢; humanHS2RT3: US, 5¢-GAGTCATGCTGAGGCTTAG GG-3¢ and...
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Báo cáo khoa học: Lack of stabilized microtubules as a result of the absence of major maps in CAD cells does not preclude neurite formation pot
... GTTGGTCTCGTCGCTCATCACATCACGAGG GCTTGAAGGCGCTGGATCTGCGACAATAG GACTGGGCTTTCATCAGCGACAGGTGGC GTGAACCACCAAAATCGGAGAACGAAGC CAGGTTCTCAGTAGAGCCAATCTTCGACCTGAC AGAGTCGGATGCAGTTGCCCGGGCAACA GGCTCCTCCAGCACCCTCCGGGTCCCG ... GGCTCCTCCAGCACCCTCCGGGTCCCG CCCCAAACTTGTGACCATCATTC GGAGAAATCATCTTGAGCATAGCG CGAACTCTCAAGGGC ATGCATCAGAACCATGCACG AGCCCTACAATTCCATCCTCACC GCTGAAGGAGACGATGAGGGTGA 82898–82923 83581–83552 91489–91517 ... in the appearance of a significant amount of acetylated tubulin (Fig 1C, and h; Fig 1D), indicating that both acetylase and deacetylase were present in CAD cells Treatment of cells with Taxol induces...
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Báo cáo khoa học: Epigenetics: the study of embryonic stem cells by restriction landmark genomic scanning pptx
... profiles of teratomas was less than that of the somatic tissues, probably because the teratomas still contained a significant number of undifferentiated proliferating cells, or all cells in teratomas ... re-methylating actions of Dnmt 3a ⁄ Dnmt3b (Fig 2) Dnmt 3a and Dnmt3b appear to function both as maintenance and as de novo methyltransferases in gene areas, and thus are crucial for the establishment of ... N Hattori and K Shiota methylation analysis Although RLGS requires a larger genomic sample than is necessary for microarray-based methods, it has advantages for analyzing genome-wide methylation...
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differentiation of embryonic stem cells
... R WATERMAN VOLUME 273 RNA Polymerase and Associated Factors (Part A) Edited by SANKAR ADHYA VOLUME 274 RNA Polymerase and Associated Factors (Part B) Edited by SANKAR ADHYA VOLUME 275 Viral Polymerases ... KITAJIMA (5), Department of Molecular Cell Biology, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita Osaka, Osaka 565-0871, Japan RAJA KITTAPPA (22), Laboratory ... Stem Cells: Hematopoietic and Vascular Cell Types STUART T FRASER, JUN YAMASHITA, L MARTIN JAKT, MITSUHIRO OKADA, MINETARO OGAWA, SATOMI NISHIKAWA, AND SHIN-ICHI NISHIKAWA 59 KENJI KITAJIMA, MAKOTO...
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báo cáo hóa học:" Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice" docx
... Yamahara, Jun Yamashita K, Takami Yurugi-Kobayashi, Akane Nonoguchi, Yutaka Suzuki, TingHsing Chao, Naoki Sawada, Yasutomo Fukunaga, Kazutoshi Miyashita, Kwijun Park, Naofumi Oyamada, Naoya Sawada, ... Sawada, Yasutomo Fukunaga, Masakatsu Sone, Kenichi Yamahara, Takami Yurugi-Kobayashi, Kwijiun Park, Naofumi Oyamada, Naoya Sawada, Daisuke Taura, Hirokazu Tsujimoto, Ting-Hsing Chao, Naohisa ... Sawada, Daisuke Taura, Naohisa Tamura, Yasushi Kondo, Shinji Nito, Hirofumi Suemori, Norio Nakatsuji, Sin-Ichi Nisikawa, Kazuwa Nakao: A pathway for differentiation of human embryonic stem cells...
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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
... CanonicalCorrelation Analysis Journal of Statistical Software 2008, 23(12):1-14 Takakura S, Mitsutake N, Nakashima M, Namba H, Saenko VA, Rogounovitch TI, Nakazawa Y, Hayashi T, Ohtsuru A, Yamashita ... 26(2):356-363 Takamizawa J, Konishi H, Yanagisawa K, Tomida S, Osada H, Endoh H, Harano T, Yatabe Y, Nagino M, Nimura Y, et al.: Reduced expression of the let-7 microRNAs in human lung cancers in association ... comparison of partially differentiated EB and terminal differentiated adult cells From miRNA array analysis, we identified a total of 104 differently expressed miRNAs that clearly segregate the...
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