derivation maintenance and induction of the differentiation in vitro of equine embryonic stem cells pdf
báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
... expression in embryonic stem cells suggest they are involved in maintaining "stemness" Identifying mRNAs that are directly targeted by a specific miRNA is a major obstacle in understanding the miRNA ... names of human embryonic stem (hES) cells lines P denoted the number of passages of the cell lines H9-EB denoted embryoid body (EB) prepared from cell line H9 and the day indicates the time in culture ... I6, H9 and BG01v are names of hES cells lines P denotes the number of passages of the cell lines H9-EB denotes embryoid body (EB) prepared from cell line H9 and the day indicates the time in culture...
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Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx
... analyzed the interaction of RNA Pol II and TATA binding protein (TBP) as well as the appearance of modified histones within the globin locus during the course of differentiation using the ChIP ... a dominant chromatin-opening activity In other words, HS3 could maintain a small accessible region in the globin locus during the differentiation of hematopoietic stem cells to erythroid cells ... TPB, and specific histone modification marks to the b-globin gene locus during erythroid differentiation of murine ES cells in vitro In these experiments we utilized the ES ⁄ OP9 cell in vitro differentiation...
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The derivation, propagation, storage and gene expression of human embryonic stem cells on human feeders
... categories of cells make up the human body viz., germ cells, somatic cells and stem cells Somatic cells include the bulk of the cells that make up the human adult and each of these cells in their ... practice involves either the injection of stem cells directly into the targeted tissue, or injection of the stem cells into the bloodstream without any guarantee that they will actually home in the ... studies in the murine model Classification of stem cells Germ cells of the gonads Mammalian stem cells are usually classified according to their tissue of origin The ovary and testis contain oogonia...
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Báo cáo y học: "Chordin knockdown enhances the osteogenic differentiation of human mesenchymal stem cells" pdf
... Biosystems, Warrington, UK) PCR was performed using the Invitrogen Platinum Taq (Invitrogen) method, in accordance with the manufacturer's instructions The PCR primers were designed on the basis of ... conducted first to determine the optimal concentration of siRNA to use in this in vitro system, and secondly to select the siRNA candidate with the highest efficiency in knocking down the target mRNA ... studies, in which the expression of BMP-2 and chordin increased with the osteogenic differentiation of MSCs in this culture model system [8,19] Einhorn and colleagues [8] also demonstrated that the...
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Directed differentiation of human embryonic stem cells into haematopoietic and definitive endodermal lineages
... studies on cells of the cardiac mesoderm showed that the timing of ingression through the streak and the position of these cells in the epiblast determines their lineage fate (Tam and Behringer 1997; ... clinical applications and ensuring acceptance of the transplant and preventing rejection by the recipient’s immune system remain Harnessing and understanding the differentiation potential of ... mouse vii LIST OF FIGURES Figure 1.1 Embryonic origin of human embryonic stem cells and their in vitro characterisation Figure 1.2 Lineage tree of embryo-derived cells and cell lines Figure 1.3...
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báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx
... Figure The combination of stem cell models and epigenomics in studies of the role of non-coding mutations in human disease Epigenomic analyses of cells derived through in vitro stem cell differentiation ... methylcytosine cells, chromatin of pluripotent cells appears to have unique features, such as the increased mobility of many structural chromatin proteins, including histones and heterochromatin protein ... understanding epigenetic differences between hESC and iPSC lines will be essential for harnessing the potential of these cells in regenerative medicine Remnants of the somatic cell epigenome in iPSCs:...
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IN VIVO EX VIVO OSTEOGENESIS OF HUMAN EMBRYONIC STEM CELLS
... understanding of the mechanism underlying the lineage specific differentiation of hES cells is mandatory 1.5 Human Embryonic stem cells As the name suggests, the human embryonic stem cells are ... regenerative therapy include (1) the adult stem cells from the adult tissue (autologous or allogenic) and from the bone marrow (mesenchymal stem cells, hematopoietic stem cells) and (2) embryonic stem and ... be eliminated very soon Other challenges in the use of ESC include controlling lineage specific differentiation and elimination of residual stem cells Thus to obtain a purified population of osteoblasts...
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Transcriptome study of human embryonic stem cells and knockdown study of a pluripotency marker, LIN28
... pluripotency in human ES cells LIN28, an RNA binding protein, contains three RNA binding domains: one coldshock domain at the N terminus and two CCHC-type zinc finger domains at the C terminus It ... 1973; Gearhart and Mintz, 1974) The pioneering work in mouse EC cells paved the way to the derivation of pluripotent cells from the ICM of mouse blastocysts, termed embryonic stem (ES) cells, under ... together as a separate group from the other tissues/cell lines regardless of the differentiation stages, Page 38 which indicated the uniqueness of the human ES cells from the rest of the cells, ...
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OCT 3 4 and SOX 2 are key factors for SDIA neurogenesis of mouse embryonic stem cells
... subsequent engineering of short, hairpin vectors under the control of Tet-R, have the advantage of being easily created for the specific gene of interest We selected the Invitrogen BlockIT Tet-inducible ... and the combinations of these factors in the maintenance of pluripotency As such, they may also elucidate the underlying “combinatorial code” specified by transactivation targets, resulting in ... providing countless days of humor and inspiration, and providing invaluable thoughts on science and life, and all things in between Table of Contents Abstract Introduction Results Culturing of Mouse...
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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
... culture within bioreactors Conflicts of interest The authors have declared that no conflict of interest exists References Becker EW The roles of ATP in the dynamics of the actin filaments of the cytoskeleton ... Inc, St Louis, MO, USA) constituted in PBS, to each well (4.8 cm2) of the 12-well dish, following by incubation for h at 37 °C in the dark After incubation, the MTT solution was removed and the ... (3×), and exposed to blocking buffer (1% BSA in PBS) for a further 30 at 37 ◦C, so as to minimize nonspecific adsorption of the antibodies After another wash in PBS (3×), the cells were incubated...
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Baculovirus mediated genetic modification of human embryonic stem cells
... modification The genetic modification of hES cells can be helpful in the differentiation and isolation of lineage-committed cells, in tracking the process of differentiation, in gene function analysis, and ... benefits of genetic manipulation include controlling differentiation of ES cells, screening and acquiring pure populations of specific types of ES cell-derived cells, altering antigenicity of cells ... Human embryonic stem (hES) cells are cells derived from the inner cell mass of human blastocysts The hES cell research began with the derivation of hES cell line and its successful culture in the...
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Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps
... formation, of 541.3 ± 166.3 μm2 in the center and 386.1 ± 108.7 μm2 the periphery of the Ad.BMP-2 transduced aggregates, and of 307.8 ± 75.6 μm2 in the center and 248.7 ± 65.4 μm2 the periphery of the ... staining for COL X in the aggregate matrix at day 10 and 21 of culture (Figure 4b) In the Ad.BMP-4-modified cultures COL X immunostaining of the matrix was strongly observed at day 21 in the ... to the joint space and the bone Page 10 of 15 (page number not for citation purposes) respectively, alignment of the chondrocytes along the arcades of Benninghoff [29] and by the appearance of...
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Báo cáo sinh học: " Protection against the allergic airway inflammation depends on the modulation of spleen dendritic cell function and induction of regulatory T cells in mice" docx
... alone The thickness of small airway walls and the number of infiltrated eosinophils around the airway increased, eosinophils appeared within the lumen of the airway, and goblet cell hyperplasia and ... cells in duplicate on coded slides BAL fluid was stored at 70°C and levels of the cytokines interleukin (IL)-4, IL-5 and interferon (INF)-γ were determined using specific ELISA according to the ... against the allergic airway inflammation depends on the modulation of spleen dendritic cell function and induction of regulatory T cells in mice Genetic Vaccines and Therapy 2010, 8:2 Page of...
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Applying macromolecular crowding to promote the expansion and adipogenic differentiation of human mesenchymal stem cells in vitro; an effect of matrix reciprocity
... 2001;Function and interactions of integrins Cell Tissue Res (3):285-298 41 Mould AP, Askari JA, Aota SI, et al 1997;Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory ... niche, self-renewal and differentiation Arthritis Res Ther (1):204 121 Owen M, Peck W 1985;Lineage of osteogenic cells and their relationship to the stromal system In ed Bone and MineralAmsterdam ... 2007 ;In vitro enhancement of collagen matrix formation and crosslinking for applications in tissue engineering: a preliminary study Tissue Eng (2):385391 37 Qian L, Saltzman WM 2004;Improving the...
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Tài liệu Báo cáo khoa học: Cold survival in freeze-intolerant insects The structure and function of b-helical antifreeze proteins pdf
... with the structure of the longer isoform CfAFP-501 using the main chain of residues Thr23fiAsn90 in isoform 337 and residues Thr54fiMet121 in CfAFP-501 (B) Overlap of isoforms 337 and 501 using the ... the interaction at the atomic level and understand the role of the threonine side chains in ice binding The contradiction between the higher activity demonstrated by the longer insert AFP isoforms ... in TH activity of CfAFP-501 Dynamics of insect AFPs To determine whether changes in temperature cause changes in the structure of the insect AFPs and to further characterize the TXT face of these...
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Báo cáo khoa học: Activation of Stat5 and induction of a pregnancy-like mammary gland differentiation by eicosapentaenoic and docosapentaenoic omega-3 fatty acids docx
... well-established family of cytoplasmic hydrophobic ligand binding proteins and are involved in binding and intracellular transport of PUFAs Human B-FABP, initially identified as a mammary gland differentiation ... DHA and EPA, in that the effect of DPA was stronger than those of EPA and DHA in suppressing tubeforming activity in endothelial cells induced by vascular endothelial growth factor [39] In the ... analyzed in the mammary gland, a significant increase in n-3 DPA and EPA in pregnant glands versus control glands is observed (Table 1) Whereas there was an abundant DHA in the virgin control gland, the...
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Báo cáo khoa học: Fatty acid composition of chylomicron remnant-like particles influences their uptake and induction of lipid accumulation in macrophages pot
... concentrations of cholesterol and TG accumulated were determined In addition, the effects of varying fatty acid composition on the uptake of the CRLPs by the cells was studied using the four types of particle ... shown to behave like the physiological lipoprotein in their clearance from the blood and metabolism in vivo, and in their effects on cultured cells in vitro [28–30] In addition, in earlier work we ... to that of palm CRLPs (Fig 3), the amount of lipid accumulated in the cells was significantly lower As the radiolabel in these experiments was in the oleate moiety of triolein, these findings suggest...
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Báo cáo khoa học: "REVERSIBLE AUTOMATA AND INDUCTION OF THE ENGLISH AUXILIARY SYSTEM" doc
... string from the first pattern will yield the remaining 17 strings of that pattern Further adding two length-four strings from the awkward second pattern will yield the remaining 18 strings of ... as for the joint corpus Table illustrates the trade-off in this case between inferential power and the proper handling of exceptions For l-reversible inference, 45 of the verb sequences of length ... For the active corpus, zero-reversible inference groups the forms of with the other modals The D F A s for the passive and full corpora also contain loops and thereby generate infinite numbers of...
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Manual on the management, maintenance and use of blood cold chain equipment potx
... Manual There are many health workers involved in the establishment and maintenance of the blood cold chain, each playing a vital role to protect the safety and efficacy of the blood They include the ... Good insulation reduces the workload on the compressor, which in turn adds to the life span of the equipment C Interior lining The internal cabinet lining of refrigerators and freezers is made of ... purposes The exterior of the cabinet is made of galvanized metal or aluminium The recommended internal lining is stainless steel The doors of the cabinet are usually of the same construction as the...
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