derivation and characterization of alveolar epithelial cells from murine embryonic stem cells in vitro pdf
Báo cáo y học: " Systems-level comparison of host responses induced by pandemic and seasonal influenza A H1N1 viruses in primary human type I-like alveolar epithelial cells in vitro" pdf
... functions of individual candidates in influenza pathogenesis will require further investigation Lack of the control of transcriptional suppression by zinc finger proteins in pdmH1N1 infected cells ... nine of the genes down-regulated in response to seasonal H1N1 influenza virus (but not pdmH1N1) encode zinc finger proteins, including ZNF175 ZNF175 contains 13 zinc fingers and a KRAB domain, ... triple-reassortant swine influenza virus acquiring a neuraminidase and matrix (M) gene segment from viruses of the Eurasian-avian-like swine virus lineage [5,6] Since these swine viruses have in turn originated...
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Báo cáo y học: " Effects of cigarette smoke condensate on proliferation and wound closure of bronchial epithelial cells in vitro: role of glutathione" pdf
... obtained after incubating cells with GSH (1.25 – mM) instead of NAC (data not shown) In contrast, in NCI-H292 cells but not in PBEC, depleting GSH using BSO resulted in a full inhibition of wound ... N-acetylcysteine (NAC) on CSC-induced epithelial wound repair in NCI-H292 and in PBEC Effect of3 Effect of N-acetylcysteine (NAC) on CSC-induced epithelial wound repair in NCI-H292 and in PBEC Mechanical ... Effect of4 Figure buthionine sulphoximine (BSO) on CSC-induced epithelial wound closure in NCI-H292 and in PBEC Effect of buthionine sulphoximine (BSO) on CSC-induced epithelial wound closure in...
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Báo cáo khoa học: "Effects of propranolol in combination with radiation on apoptosis and survival of gastric cancer cells in vitro" ppsx
... NY) containing 10% (v/v) heat-inactivated fetal bovine serum (Gibco, Grand Island, NY), penicillin (100 U/mL) and streptomycin (100 mg/mL), and they were maintained in a 37°C humidified incubator ... Analysis of the data and plotting of the figures were performed with the aid of software (Origin Version 7.5 and SPSS Version 13.0) Results Expression of b1- and b2-adrenergic receptors in SGC7901 and ... of Figure Apoptosis induction by isoproterenol or propranolol in combination with irradiation in BGC-823 and SGC-7901 cells There was an increasing rate of apoptosis in gastric cancer cell lines...
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Báo cáo khoa học: Identification and characterization of 1-Cys peroxiredoxin from Sulfolobus solfataricus and its involvement in the response to oxidative stress pdf
... redox-active cysteine, the resolving cysteine (Cys-SR) In typical 2-Cys, Sp at the N terminus and SR at the C terminus of the protein belong to different subunits and condense to form an intersubunit ... terminus and SR at the C terminus belong to different subunits and condense to form an intersubunit disulfide bond; in atypical 2-Cys, Sp at the N terminus and SR at C terminus, originate from ... utilized in the northern analysis (Fig 10) Twenty-five kDa signals corresponding to Bcp2 were detected in noninduced and induced cells The increased amount of Bcp2 in the cytoplasmic fraction of S...
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Báo cáo khoa học: Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL family docx
... Biochem 271) model is in agreement with our CD data showing two minima at 205 and 215 nm, indicating that Rv1399c is composed of 22% of a-helices, 25% of b-strand and 39% of random coil The catalytic ... different ratio of E600 In each case, the kinetics of inactivation of Rv1399c have been monitored by measuring the remaining activity, as a function of time, using tripropionin as a substrate ... obtained from the pDest 17O/I construct using the BL21 (DE3)pLysS cells grew in LB medium after h of IPTG induction (Fig 1) Purification and refolding of Rv1399c Given the large quantity of insoluble...
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Báo cáo Y học: Identification and characterization of a new gene from Variovorax paradoxus Iso1 encoding N -acyl-D-amino acid amidohydrolase responsible for D-amino acid production pdf
... methionine residues were Met39, Met171, Met254, Met273 and Met352 It is known that the common sites of oxidation in proteins are histidine, lysine, proline, cysteine, arginine and methionine residues ... paradoxus strains By using the neighbour-joining, maximum-parsimony and maximumlikehood (Fig 1) methods from PHYLIP and testing the resulting trees using bootstrap analysis, the strain Iso1 specifically ... were from New BioLabs and Gibco BRL Pfu DNA polymerase and alkaline phosphatase were from Promega and Boehringer Mannheim, respectively D-Amino acid oxidase (EC 1.4.3.3) from porcine kidney and...
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Báo cáo khoa học: Genomic structure, expression and characterization of a STAT5 homologue from pufferfish (Tetraodon fluviatilis) ppt
... N-terminal protein interaction domain, coiled-coil domain, DNA-binding domain, SH2 domain, and C-terminal transactivation domain Furthermore, a tyrosine (Y698) was also identified in the C-terminal transactivation ... identical The boundaries of N-terminal protein interaction domains, coiled-coil domains, DNA-binding domains, linker regions and SH2 domains are indicated by arrowed brackets The tyrosine residue phosphorylated ... DNA-binding activity in vivo Fig Phylogenic tree of Tf STAT5 and other STAT family members The amino acid sequences of DNA-binding and SH2 domains of Tf STAT5 were aligned with those of nine known...
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Báo cáo hóa học: " Detection and characterization of chicken anemia virus from commercial broiler breeder chickens" pdf
... percentage of positive DNA from spleen, thymus and bone marrow make these organs an excellent choice of organs in the screening and diagnosis of flocks for CAV infection The finding of CAV DNA in embryos ... I and II (Fig 2) Seven of the isolates with amino acid profile of 75-I, 97-L, 139-Q and 144-Q were clustered together in cluster I while the remaining five isolates with amino acid profile of ... CIA-1 from USA, CAV-B from India, BD-3 from Bangladesh, isolate 704 from Australia, isolate 130 from Slovenia and NIE/19.04/118 isolate from Nigeria The remaining five current isolates including...
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Báo cáo hóa học: " Identification and characterization of alkaline serine protease from goat skin surface metagenome" docx
... and location of protease gene 4Fe-4S represents 4Fe-4S ferredoxin iron-sulfur binding domain protein, S8 & S53 - peptidase S8 and S53 subtilisin kexin sedolisin, sterol - Sterol-binding domain ... found in different strains of Shewanella, suggesting that the insert from metagenome could have been derived from a strain of Shewanella sp Majority of Shewanella sp are of marine origin (Fredrickson ... autoactivation of the recombinant cysteine proteinase EhCP112 from Entamoeba histolytica Protein Expr Purif 63:26–32 Page 10 of 10 Radha S, Gunasekaran P (2007) Cloning and expression of keratinase gene in...
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Báo cáo hóa học: " Preparation and Characterization of Nano structured Materials from Fly Ash: A Waste from Thermal Power Stations, by High Energy Ball Milling" potx
... blocks and tiles, in road making, in filling of the mines Attempts have been made earlier to utilize this fly ash waste in the polymer industry in making polymeric composites where fly ash is being ... analysis are shown in Table The percentage of alumina reduces marginally and the percentage of silica increases marginally as milling for 20 h, there after it remains unaffected with milling time TiO2 ... decreases and those of CaO and Fe2O3 marginally increased with long hours of milling Particle Size, Surface Area and Surface Energy Measurements of Fly Ash The variation in particle size and specific...
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Báo cáo khoa học: "Purification and characterization of two larval glycoproteins from the cattle tick, Boophilus annulatus" doc
... rof g × 000,51 ta degufirtnec dna ,detacinos ,deretlif neht erew dna ,srotibihni esaetorp liatkcoc gniniatnoc ,2.7 Hp ,ATDE muidosid Mm dna )SBP( enilas dereffub-etahpsohp M 51.0 dloc ni dezinegomoh ... enomleilguG ,JA dlognaM ,MM ingoploV ,MM enurfaC ,OA nitalaS ,EA labañiV ,HD erriugA secnerefeR ASU ,sionillI fo ytisrevinU ,)AICUM( seitivitcA lanoitanretnI rof muitrosnoC seitisrevinU tsewdiM eht morf ... lacitirc rof ,tpygE ,oriaC ,ertneC hcraeseR lanoitaN ehT ,tnemtrapeD ygoloiB raluceloM ,demaH adeR aagaR rD knaht eW secneuqes dica onima lanimret-N gninimreted rof ,kramneD ,ytisrevinU kramneD...
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Báo cáo khoa học: " Isolation and characterization of canine umbilical cord blood-derived mesenchymal stem cells" pps
... shown in pellet formation and toluidine blue staining In this study, we used cUCB-MSCs at 3∼5 passage Generally, increasing the passage number of adult stem cells often leads to a decline in the ... in isolating enough multipotent stem cells from human umbilical cord blood and maintaining cell culture for experimental analysis A guarantee of enough numbers of multipotent stem cells out of ... bottom of the tube (Fig 3D), and positive to toluidine blue staining (Figs 4E and F) Discussion Isolation and characterization of stem cells derived from various tissues and sources have been one...
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Báo cáo y học: "Early Induction of apoptosis with tobacco smoke and related products in A549 lung epithelial cells in vitro" potx
... number of cells detaching was measured in the cell supernatant Cells were grown in 24 well plates at a seeding density of 105 cells/ ml in complete media overnight; cells were washed in PBS and treated ... 0.05) increase in the number of apoptotic cells (Figures 2a and 2b) While treatment with Taxol also caused a significant (p < 0.05) increase in the number of "indeterminate" cells Interestingly, ... detachment of cells, DNA strand breaks, and reduced surfactant production [58] Breaking of DNA strands has been associated with apoptosis of alveolar cells There is conflicting evidence for the induction...
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Báo cáo khoa học: "Expression and characterization of the UL31 protein from duck enteritis virus" pot
... Figure Intracellular location of DEV UL31 protein analyzed by indirect immunofluorescence Intracellular location of DEV UL31 protein analyzed by indirect immunofluorescence Mock and DEV-infected cells ... penicillin and 100 μg/ml streptomycin and were used throughout this study DEV CHv strain was a high-virulence field strain isolated from china, obtained from Key Laboratory of Animal Disease and ... location of the UL31 product in DEV-infected cells The intracellular distribution of UL31 protein was examined by indirect immunofluorescence staining At 36 h Page of 10 (page number not for citation...
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Báo cáo y học: " Calcitonin gene-related peptide stimulates proliferation of alveolar epithelial cells" docx
... before being finally maintained in 100% ethanol at 4°C The lung tissue was embedded in paraffin and cut into each μm section, and Hematoxylin-Eosin (HE) staining and immunohistochemical staining for ... proliferation of alveolar epithelial cells in vivo and in vitro cannot be generalized In the present in vivo experiment, proliferation of alveolar epithelial cells was actually observed in the rat lung in ... inhibited the increase of BrdU incorporation (Fig 4) Effect of recombinant human CGRP vector in rat lung Proliferation of alveolar epithelial cells and interstitial thickening were observed in...
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Báo cáo sinh học: "Construction and characterization of a BAC library from a gynogenetic channel catfish Ictalurus punctatus" pps
... areas of the genome may be over- or under-represented due to variation in HindIII sites in the selected range of insert sizes, or the inability to clone/maintain certain catfish genomic regions in ... genetic linkage map contains 243 type II (noncoding) and only 20 type I (coding) markers in 32 linkage groups, with an average inter-marker distance of 8.7 cM [28] However, a physical map of the ... g for 15 min, resuspended in 1.65 mL of Buffer L (100 mM EDTA, 20 mM NaCl, 10 mM Tris-HCl pH 8.0) containing 0.6% low melting agarose (InCert agarose, BMA, Rockland, ME), and poured into 75 µL...
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Tài liệu Báo cáo Y học: Identification and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx
... members of this family including UspA itself (Fig 2), and one larger protein consisting of two UspA domains in tandem [17] The small members of this family are proteins of 142–144 amino-acids ... growth and expression of UP12 Absorption (upper panel) and relative amount of UP12 (lower panel) in samples of cells grown in minimal M9 medium containing limited concentration of glucose (m) or in ... 12 samples of cells taken at the indicated time (B) Western blot analysis of proteins in samples collected in (A) lg and lg of total protein in samples were separated by SDS/PAGE and immunoblotted...
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Báo cáo Y học: Purification and characterization of VanXYC, a D,D-dipeptidase/D,D-carboxypeptidase in vancomycin-resistant Enterococcus gallinarum BM4174 docx
... the extent of interference of precursors terminating in D-Ser on the rate of hydrolysis of UDP-MurNAc-pentapeptide[Ala] was measured using HPLC The rate of removal of the terminal D-Ala of mM UDP-MurNAc-pentapeptide[Ala] ... of terminal D-Ala from UDP-MurNAc-pentapeptide[Ala] in the presence of D-Ala-D-Ser was not clear from an examination of their Km values, being 17 mM and 15 mM, respectively, as determined using ... for kinetic studies when the modified cadmium-ninhydrin method [17] was used, with D-alanine or D-serine as standards The sensitivity ranges of the D-amino acid oxidase and cadmium ninhydrin assays...
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Báo cáo hóa học: " Synthesis and characterization of aligned ZnO/ BeO core/shell nanocable arrays on glass substrate" pdf
... sample originates from the type I band alignment between ZnO and BeO, in which the valence band maximum of BeO is of lower energy than that of ZnO, while the conduction band minimum of BeO is of higher ... frame in (b) island growth will occur to release internal stress, and the grains tend to grow in random orientation to minimize the surface energy Correspondingly, a polycrystalline shell and a ... images of ZnO nanowires synthesized with different solution composition, as the Page of amount of zinc nitrate in the solution increases from 0.5 to mmol, and increase in the average diameter of...
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Báo cáo khoa học: " No supra-additive effects of goserelin and radiotherapy on clonogenic survival of prostate carcinoma cells in vitro" ppsx
... induced by goserelin incubation and irradiation in LNCaP cells In PC-3 cells, we found more expression of this protein after incubation with goserelin than after irradiation alone Goserelin incubation ... combination of goserelin with radiotherapy in PC cannot be attributed to an increase in radiosensitivity of PC cells by goserelin in vitro It is to be explained by the synergistic effects of AD and ... to explain the clinically observed increase of overall survival in patients after combined therapy Recent studies investigated the effects of AD and radiotherapy in PC cell lines in vitro [summarized...
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