data protein interaction data and ontology based literature searches
gene therapy for liver disease
... galactose-terminated glycoproteins and neoglycoproteins with high affinity Bound ligands are internalized by the cell via receptor-mediated endocytosis Due to its specificity, the asialoglycoprotein receptor ... Posttranslational processing results in the RNA binding nucleocapsid protein C, the envelope proteins E1 and E2, and the nonstructural proteins NS1 to NS5, including RNA-dependent RNA polymerase At ... delivery of protein DNA complexes is the use of asialoglycoprotein receptors The asialoglycoprotein receptor is present in large numbers only on the plasma membrane of hepatocytes and binds galactose-terminated...
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Báo cáo sinh học: "AAV-mediated gene therapy for metabolic diseases: dosage and reapplication studies in the molybdenum cofactor deficiency model" pps
... out the practical work and drafted the manuscript WW participated in the practical work and discussions JR designed this study and edited the manuscript All authors read and approved the final ... the EGFP expression cassette in all cells after microinjection of fertilized oozytes and subsequent breeding (data not shown), which indicates that not the rate of transduction but rather persistence ... described previously [5] and above, the results described here indicate a similar efficacy for both application schemes All five above described mice had been mated and were fertile The offspring...
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Báo cáo khoa học: Active-site-specific chaperone therapy for Fabry disease Yin and Yang of enzyme inhibitors pptx
... Gaucher disease [20,21], Tay-Sachs and Sandhoff disease [22] (details for Gaucher and Tay-Sachs ⁄ Sandhoff diseases are reviewed separately), GM1-gangliosidosis [23], and retinitis pigmentosa 17 [24] ... N215S, Q279E, M296I, and M296V), four mutations found in classic patients (E59K, A156V, L166V, and R356W), and two mutations present in both variant and classic patients (A97V and R301Q) were efficiently ... with D92, D93, K168, E203, R227, and E231; and a hydrophobic surface on DGJ makes van der Waals interactions with W47 (Fig 4) The binding between DGJ and the protein would fix the active-site...
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gene therapy for hematological disorders
... factors such as flt-3 ligand and megakaryocyte growth and development factor (MGDF)/thrombopoietin (TPO) have augmented the level of genetically modified cells These cytokines and growth factors maintain ... retroviral vector containing Neo and human ADA genes in both murine and human T lymphocytes Transduced murine lymphocytes could be selected by growth in G418 and subsequently expanded without changing ... cells However, CD24 and HSA are glycosylphophatidylinositol-linked surface proteins This class of proteins has been shown to be transferred from cell to cell both in vitro and in vivo, possibly...
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gene therapy in cardiovascular disease
... E2F is bound to a complex of other proteins, including a protein known as the retinoblastoma (Rb) gene product Rb prevents E2F’s interaction with chromosomal DNA and stimulation of gene activity ... GRAFTS 193 FIGURE 8.4 Control oligonucleotide-treated (A and B) and antisense oligonucleotide (against c and kinase/PCNA)-treated vein grafts (C and D) in hypercholesterolernic rabbits, weeks after ... of sulfhydryl groups of intracellular and membrane proteins, nucleic acid injury, and breakdown of components of the extracellular matrix such as collagen and hyaluronic acid Natural oxygen radical...
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gene therapy for hiv infection
... the viral proteins The HIV genome encodes three sets of viral proteins; the structural proteins (Gag, Pol, and Env), the regulatory proteins (Tat, Rev, and Nef), and the maturation proteins (Vif, ... classes of viral proteins are generated from these mRNAs: structural proteins, regulatory proteins, and maturation proteins The structural proteins include the viral envelope protein (gp 120, ... the physical interaction of the HIV envelope glycoprotein gp120 and the CD4 protein Based on these results, investigators have demonstrated that sCD4 protein can effectively bind to and inhibit...
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Báo cáo y học: " Chitosan Interferon-c Nanogene Therapy for Lung Disease: Modulation of T-Cell and Dendritic Cell Immune Responses" pps
... Materials and Methods, and CD11c+ cells were isolated from BAL fluid and lymph nodes Cells were analyzed by flow cytometry for the expression of CD40, CD80, CD86, and I-Ad gated to CD11c+b+ The data ... mice were euthanized and the lungs were removed and fixed in buffered formalin Whole lungs were embedded in paraffin, sectioned, and examined for GFP by fluorescent microscopy, and an approximate ... were treated therapeutically with CIN and then challenged with OVA and euthanized 18 hours later Lungs were removed, and single-cell suspensions were prepared and plated as described above In this...
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Báo cáo y học: " Cell therapy for autoimmune diseases" ppt
... to normalize and returned to pretreatment levels before relapse In SLE patients, antinuclear antibody and antidouble-stranded DNA antibodies disappeared in many patients after HSCT and returned ... cytokineindependent and antigen-independent naturally occurring population, and another cell type that is recruited by the cognate antigen and immunoregulatory cytokines and thus named adaptive ... of ‘salvage and respite’, as in the case of acute GvHD These and other issues such as optimal expansion media (for example, animal protein free, platelet lysate, autologous serum) and the source...
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báo cáo khoa học: "Reconsidering low-dose aspirin therapy for cardiovascular disease: a study protocol for physician and patient behavioral change" pot
... support aid for clinicians, and a patient activation form; and Test the effectiveness of these interventions to improve the evidence based use of aspirin for primary and secondary prevention of ... Colorado Hospital’s Allscripts database and the QED Clinical, Inc (DBA-CINA) Clinical Data Repository maintained by the Department of Family Medicine Our post-intervention data will be collected using ... activation form and helped to draft the manuscript WP participated in the design of the study and the patient activation form and helped to draft the manuscript All authors read and approved the...
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Báo cáo y học: " Taking stock of gene therapy for cystic fibrosis" pdf
... and containing numerous submucosal glands, and the small bronchiolar regions lined by a simple columnar epithelium devoid of glands A central question for CF gene therapy is which cell type and ... prolonged expression of the protein and positive changes in clinical endpoints, including circulating levels of factor IX and the frequency of infusion of factor IX protein The questions that ... superficial epithelium is abundant and displays the ion transport defects in patients with CF, the submucosal glands are the cells expressing the highest CFTR levels in the lung and might well need to be...
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Báo cáo sinh học: "Careful adjustment of Epo non-viral gene therapy for β-thalassemic anaemia treatment" pot
... contributions YB, DS, PB and EP carried out the design of the study EEF and EP performed experimental protocols, assays and data collection All the authors participated in data analysis EEF drafted ... firsts, the third and the fourth electrotransfers of the 1.5 μg Epoplasmid dose These hematocrit values were of 42.3%, 41.6% and 41.8%, and delta hematocrit values were of 9.0%, 9.0% and 9.8% respectively ... day 91 and then from day 189 to day 271 for the 1.5 μg group (p values of 0.002 on day 91, 0.005 on day 189, 0.002 on day 210, 0.01 on day 241 and 0.002 on day 271) and at day 91, 189 and 241...
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The natural history of non-alcoholic fatty liver disease in children: a follow-up study for up to 20 years pptx
... liver/kidney microsome type 1, and anti-mitochondrial antibody), and a standard metabolic/inborn error panel (lactate/pyruvate ratio, urine and serum organic acids and amino acids) The REP master ... was determined according to age and sex based on data from the Center for Disease Control and Prevention.24 Obesity was defined by a BMI 95th percentile for age and sex.24 Abnormalities in the ... daily ethanol consumption of less than 10 g; and (3) appropriate exclusion of other liver diseases based on standard clinical, laboratory, imaging and/ or liver biopsy features Laboratory tests...
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Gene Transfer Approaches for Gynecological Diseases pot
... clinical trial In vitro and in vivo Adenovirus Ad5/3-D24 Main result In vitro in vivo In vitro in vivo In vitro in vivo In vitro in vivo In vitro in vivo and and and and and Killing of ovarian ... (Table 2) A plasmid -based strategy with HSV-TK/GCV was assessed in vitro both in human clinical samples and in a rat leiomyoma cell line A bystander effect was demonstrated, and interestingly, ... to perform extensive sampling and biopsies in phase I trials to acquire material for correlative studies Obviously, this is hampered by compliance and cost issues and the fact that traditionally...
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Obesity and Air Pollution: Global Risk Factors for Pediatric Non-alco- holic Fatty Liver Disease potx
... insulin resistance and NAFLD, and similar risk factors and protective factors for these interrelated disorders Waist circumference and the ratio of apolipoprotein B to apolipoprotein A-I (ApoB/ApoA-I ... children is difficult Because of the aforementioned limitations and controversies in the diagnosis of NAFLD in children and adolescents, data based on surrogate markers might underestimate or overestimate ... Obesity and Air Pollution diagnosis, and pathogenesis of pediatric NAFLD, as well as the possible associations with obesity and air pollution, which are the adverse effects of urbanization and globalization...
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nonviral vectors for gene therapy, methods and protocols
... molecular weights and compositions (comonomer fraction up to 66 mol%) are able to bind DNA, yielding polyplexes (14,15; and Bos and Hennink, unpublished data) However, for random copolymers of ... 3.3.), and by NMR For 1H-NMR assignments, see Subheading 3.1.8 and Notes and 3.3 GPC of pDMAEMA Homo- and Copolymers This instruction is intended for the molecular weight determination of pDMAEMA and ... Sugihara, G (1996) Design and synthesis of amphiphilic -helical model peptides with systematically varied hydrophobic-hydrophilic balance and their interaction with lipid- and bio-membranes Biochemistry...
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viral vectors for gene therapy
... Methods and Protocols, edited by Curtis A Machida, 2003 60 Interleukin Protocols, edited by Luke A J O’Neill and Andrew Bowie, 2001 75 Lung Cancer: Volume 2, Diagnostic and Therapeutic Methods and ... Methods and Protocols, edited by Andrew J Pollard and Martin C J Maiden, 2001 50 Colorectal Cancer: Methods and Protocols, edited by Steven M Powell, 2001 66 Meningococcal Vaccines: Methods and Protocols, ... Faculty of Medical and Health Sciences, The University of Auckland, Auckland, New Zealand MARKUS U EHRENGRUBER • Brain Research Institute, University of Zurich, Zurich, Switzerland JOHN F ENGELHARDT...
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Báo cáo hóa học: "Anti-viral state segregates two molecular phenotypes of pancreatic adenocarcinoma: potential relevance for adenoviral gene therapy" pdf
... in green genes (ISGs), IFN induced proteins (IIPs), IFN associated signaling pathway molecules, such as JAK and STAT and IFN associated proteins, such as IL18 and OAS molecules (additional file ... quality and concentration were assessed using Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA) First- and second-strand cDNA were synthesized from 12.5 μg of Ad5-CMV-GFP and Ad5-CMV-null ... set of Ad DNA standards (449B plasmid) Plasmids for constructing pISRE-SEAP and pIFN-betaSEAP, and pMetLuc-Control were obtained from Clontech Secreted alkaline phosphatase (SEAP) and secreted luciferase...
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Báo cáo hóa học: " Abstract Despite new additions to the standard of care therapy for high grade primary malignant brain tumors, the prognosis for patients with this disease is still poor." ppt
... cells and GM-CSF (TVAX) Later, autologous T cells are harvested and expanded ex vivo, and then administered intravenously Pilot clinical trials showed promising results with this approach to expand ... conceived, outlined the direction of, and drafted the manuscript MJH, CCM and KLE refined the search for information, gathered references and generated the tables and figure MRJ, RMP, LML provided ... being vaccinated with the heat shock protein peptide complex (HSPPC)-96 with or without concurrent TMZ therapy Heat shock proteins (HSP) are highly conserved proteins that are transiently expressed...
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Báo cáo sinh học: "Comparisons of three polyethyleneimine-derived nanoparticles as a gene therapy delivery system for renal cell carcinoma" doc
... the experiments and wrote the paper draft; LS conceived, instructed the experiments and revised the paper; SG and XX performed experiments and analyzed data; ZX and ZP collected and validated tissue ... cells, and CD indicated viability of HUVEC cells respectively treated with PCFC-g-PEI and FA-PEAs, which was respectively compared with 25-kD and 2-kD PEI The error bars represented the standard ... image that used PCFC-g-PEI, FA-PEAs and HPEI as a gene delivery vector respectively And figure B, D and F represented the transfection image with 25-kD PEI and 2-kD PEI as gene vectors Figure...
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Báo cáo sinh học: "Safety and feasibility of third-party multipotent adult progenitor cells for immunomodulation therapy after liver transplantation–a phase I study (MISOT-I)" doc
... Inc., Cleveland, Ohio, USA) to be used in this study is isolated from a single bone marrow aspirate and cultured with heat inactivated fetal bovine serum (FBS) and growth factors EGF and PDGF Cells ... Pre- and Intraoperative Data Patients enrolled in this study will not need to undergo additional screening visits or clinical investigations in addition to standard pre-transplant work-up Standardof-care ... abdominal computed tomography with intravenous and oral contrast, pulmonary function tests, and arterial blood gas analysis Intraoperative data (warm and cold ischemia Popp et al Journal of Translational...
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