cruzi trypomastigotes and 24 h supernatants of trypomastigotes decrease hiv 1 replication in bewo cells
Báo cáo y học: "Oral keratinocytes support non-replicative infection and transfer of harbored HIV-1 to permissive cells" ppsx
... packaging [ 41] TERT-2 cells clearly restrict HIV replication after integration when infected with HIV- 1 We sought to determine whether the internalization pathway used by HIV- 1 in TERT-2 cells ... 77 :10 119 -10 12 4 Freed EO: HIV- 1 and the host cell: an intimate association Trends Microbiol 2004, 12 :17 0 -17 7 Gomez C, Hope TJ: The ins and outs of HIV replication Cell Microbiol 2005, 7:6 21- 626 ... new HIV- specific transcripts, including multiply spliced HIV- 1 RNA, unspliced HIV- 1 RNA, and U3-U5 HIV- 1 RNA in TERT-2 cells TERT-2 cells were incubated with HIV- 1 IIIb or BaL for h, trypsinized,...
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Báo cáo y học: " Dual effect of the SR proteins ASF/SF2, SC35 and 9G8 on HIV-1 RNA splicing and virion production" doc
... 2005, 2:33 http://www.retrovirology.com/content/2 /1/ 33 A VIRION CAp24 (ng/µl) 0,20 HIV- 1 HIV- 1 + ASF/SF2 HIV- 1 + SC35 0 ,15 HIV- 1 + 9G8 0 ,10 0,05 1: 0.5 1: 1 1: 3 1: 0.5 1: 1 1: 3 +9G8 1: 3 +SC35 1: 1 +ASF/SF2 ... are in agreement with those obtained in HeLa cells using a truncated non-infectious HIV- 1 DNA construct [26] and showed that SR proteins profoundly changed the HIV- 1 splicing pattern However the ... production and infectivity in a human cell line expressing infectious HIV1 In the present study we show that overexpression of one of the three SR proteins ASF/SF2, SC35 and 9G8 together with HIV- 1 strongly...
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Báo cáo y học: "Fully-spliced HIV-1 RNAs are reverse transcribed with similar efficiencies as the genomic RNA in virions and cells, but more efficiently in AZT-treated cells" ppt
... pNL HIV Mock HIV Mock HIV Mock HIV Mock HIV Mock HIV Mock U3 U3 FL FL FSpl FSpl DNA produced in 10 5 cells Figure analysis of reverse transcription of spliced and unspliced HIV RNAs within infected ... and FSpl RNAs in HIV- 1 infected cells induced a strong decrease (18 5-fold) of the FL cDNA amounts in infected cells In contrast, AZT induced only a 3-fold decrease of the "spliced cDNA" synthesis ... then escaping the AZT treatment The mechanism of AZT inhibition has been well studied both in vitro [7] and in infected cells [8], showing that inhibition occurred most efficiently when the DNA...
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Báo cáo khoa học: Adaptability and flexibility of HIV-1 protease potx
... 1A94, 1HWR, 1HVR, 1QBS, 1DMP, 1QBR and 1QBT) are of complexes between different types of inhibitor molecules and HIV- 1 protease containing the C95A mutation In two of these structures (1DAZ and 1A94), ... structures shown in Fig 2B 1LV1 1LV1 1HWR 1DMP 1QBS 1HVR 1QBR 1QBT 1HWR 1DMP 1QBS 1HVR 1QBR 1QBT 0.00 0.39 0.00 0.37 0.38 0.00 0.34 0.39 0.32 0.00 0.32 0. 41 0.36 0. 31 0.00 0.35 0. 41 0.33 0.33 ... 1A94), the protein is complexed with a peptide inhibitor [6,7] and in the remaining six (1HWR, 1HVR, 1QBS, 1DMP, 1QBR and 1QBT) the enzyme is complexed with a cyclic urea-based inhibitor [8 13 ] All...
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Báo cáo hóa học: " A predominance of R5-like HIV genotypes in vaginal secretions is associated with elevated plasma HIV-1 RNA levels and the absence of anti-retroviral therapy" docx
... usage of non-syncytium-inducing primary HIV- 1 is independent of phylogenetically distinct global HIV1 isolates: delineation of consensus motif in the V3 domain that predicts CCR-5 usage Virology 19 98, ... measured HIV- 1 RNA levels in vaginal secretions and plasma, determined the V3 genotype of predominant variants found in vaginal secretions, and assessed ART use in a cohort of 43 women attending an HIV ... interests 10 11 12 UNAIDS: 2007 AIDS Epidemic Update Global Summary of the HIV and AIDS Epidemic 2007 Quinn TC, Overbaugh J: HIV/ AIDS in women: an expanding epidemic Science 2005, 308(5728) :15 82 -15 83...
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báo cáo hóa học:" The HIV-1 Non-subtype B Workgroup: An International Collaboration for the Collection and Analysis of HIV-1 Non-subtype B Data" pot
... 2005, 7: 71 10 11 12 13 14 15 http://www.jiasociety.org/content/7 /1/ 71 Kantor R, Katzenstein D, Camacho R, et al.: Genotypic analyses of RT and protease sequences from persons infected with nonsubtype ... with non-B HIV- 1 subtypes Currently, the workgroup consists of investigators from 15 sites in 13 countries The goal of the workgroup is to collect and analyze a robust database of sequences and ... antiretroviral therapy in the developing world increases within the next few years, and as migration and travel lead to a rise in non-B infected persons in the developed world Extensive interlaboratory...
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Báo cáo y học: " Lentiviral transduction of Tar Decoy and CCR5 ribozyme into CD34+ progenitor cells and derivation of HIV-1 resistant T cells and macrophages" pdf
... AIDS Research and Therapy 2004, 1: 2 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 Anderson J, Banerjea A, Akkina R: Bispecific short hairpin siRNA constructs targeted to CD4, CXCR4, and CCR5 ... transduction and sustained expression of HIV- 1 interfering genes, anti-CCR5 ribozyme and HIV- 1 Page of 11 (page number not for citation purposes) AIDS Research and Therapy 2004, 1: 2 http://www.aidsrestherapy.com/content /1/ 1/2 ... challenged with HIV1 , transgenic cells showed marked resistance against HIV1 infection These results showed for the first time that expression of these transgenes in combination not interfere with normal...
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Báo cáo y học: "Clade, Country and Region-specific HIV-1 Vaccines: Are they necessary" pptx
... the James B Pendleton Charitable Trust and the American Lebanese Syrian associated Charities (ALSAC) References 10 11 12 13 14 15 16 17 18 Perrin L, Kaiser L, Yerly S: Travel and the spread of ... to human immunodeficiency virus type (HIV- 1) clades: relevance to HIV- 1 vaccine trials in Uganda J Infect Dis 2000, 18 2 :13 50 -13 56 Moore JP, Cao Y, Leu J, Qin L, Korber B, Ho DD: Inter- and intraclade ... expressing multiple envelopes of HIV- 1 Eur J Clin Microbiol Infect Dis 2004, 23 :10 6 -11 0 Hurwitz JL, Slobod KS, Lockey TD, Wang S, Chou T-HW, Lu S: Application of the polyvalent approach to HIV- 1 vaccine...
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Báo cáo y học: "Pre-clinical development as microbicide of zinc tetra-ascorbo-camphorate, a novel terpenoid derivative: Potent in vitro inhibitory activity against both R5- and X4-tropic HIV-1 strains without significant in vivo mucosal toxicity" docx
... DC T cells MΦ* DC* T cells* C14 >10 >10 >10 HIV- 1Ba-L 1. 3 ± 01 HIV- 1NDK 0.02 ± 0.0 HIV- 1Ba-L 1. 3 ± 0 .1 HIV- 1NDK 1. 8 ± 0 .1 HIV- 1Ba-L 0.8 ± 0.0 HIV- 1NDK 0.7 ± 0 .1 Enfuviritid (T20) >10 >10 >10 0.08 ... infection of macrophages, DC and T cells Therefore, HIV- 1 sensitive cells were incubated with R5 -HIV- 1Ba-L or X4 -HIV- 1NDK in the presence of increasing non-toxic doses of C14 (Table 1) C14 inhibited ... inhibit the entry of HIV- 1 into T cells and may limit the reverse transcription step into macrophages and DC These findings indicate that C14 harbours potent HIV- 1 entry inhibition activity and/ ...
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Báo cáo y học: " Detection of HIV-1 RNA/DNA and CD4 mRNA in feces and urine from chronic HIV-1 infected subjects with and without anti-retroviral therapy" pptx
... presence of HIV- 1 in urine The presence of anti -HIV- 1 antibodies has been reported in urine by ELISA and Western blot [15 ,16 ] and HIV- 1 DNA has been detected in urine pellets from HIV- 1 infected individuals ... in GALT, HIV- 1 and CD4+ T cells shed into the intestinal lumen and the amount of HIV- 1 and CD4+ T cells in the feces would associate with the pathogenic changes in GALT Since the components of ... negative in the occult blood test Thus, HIV- 1 RNA/DNA detected in the fecal samples could be the result of the shedding of HIV- 1 infected cells and/ or free virus from GALT into the intestinal lumen HIV- 1...
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báo cáo khoa học: "PVP-coated silver nanoparticles block the transmission of cell-free and cell-associated HIV-1 in human cervical culture" ppsx
... HeLa-CD4-LTR-β-gal cells and the viral strains HIV- 1IIIB and HIV- 1AZT-RV HIV- 1IIIB was propagated by sub-culturing in the MT-2 and H9 + cells, according to the DAIDS Virology Manual for HIV Laboratories ... participated in the conception and experimental design of the in vitro HIV- 1 manipulation and infection assays, in the analysis and interpretation of the data, and in the writing and revision of this ... infection of subepithelial cells of the human cervical tissue Consequently, an agent that prevents inflammation should inhibit the transmission of HIV infection by impeding the enhancement of HIV infection...
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Báo cáo y học: " Synergistic effect of human CycT1 and CRM1 on HIV-1 propagation in rat T cells and macrophages" docx
... propagation of HIV- 1 TZM-bl cells were used to measure the infectivity of HIV- 1 according to previously described procedures [26] NR8383hCRM1, FPM1hCRM1, FPM1hCT, and FPM1hCT/hCRM1 expressing hCRM1, hCycT1, ... stable than rCRM1-HA (Fig 1C), partly accounting for the lower amounts of hCRM1 (See Fig 1B) To examine the effects of both hCycT1 and hCRM1 on HIV- 1 propagation in rat T cells, including FPM1 and ... relatively high HIV- 1 LTR activity in primary macrophages [7 ,16 ,17 ] Since rCycT1, like mCycT1, has a tyrosine at residue 2 61 in place of the hCycT1 cysteine [7], which is crucial for binding to the TAR...
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Báo cáo y học: " Multiple-infection and recombination in HIV-1 within a longitudinal cohort of women" potx
... Persisted Max No of Possible Initial Prop Visits 1, 5 0.80 44 12 49 50 54 1* 1* 58* env env env pol pol env env* env* env* 1 1 1 1 1, 6 1, 4 ,11 1, 3,5 1, 5 1, 2,8 1, 1, 10 1, 10 1, 3 1 1 1 1 3 2 2 0.80 0.20 ... 0.50 0.22 39 pol 1, 7 1 1.00 0 43 45 pol env 10 2,3 1, 5,6 ,10 1 1 1. 00 1. 00 15 pol 1, 4 1 0.20 1 20 13 17 55 pol pol env pol 1, 4,9 1, 1, 6 1, 2 1 1 1 1 0.20 0.50 0.80 0.20 Average: Superinfected at last ... superinfection [11 ,13 ,14 ] In this study we examine a longitudinal cohort of HIV- 1 positive women coupled with genetic screens of the pol and env genes of HIV- 1 To enhance power to detect coinfection...
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Báo cáo y học: "Association of chemokine receptor gene (CCR2CCR5) haplotypes with acquisition and control of HIV-1 infection in Zambians" pdf
... (8.8) 11 (1. 7) 67 (10 .2) HHD 370 (16 .3 19 0 (16 .8) 18 0 (15 .9) 75 (15 .6) 10 5 (16 .1) HHE 310 (13 .7) 13 8 (12 .2) 17 2 (15 .2) 61 (12 .7) 11 1 (17 .0) HHF *1 128 (5.6) 76 (6.7) 52 (4.6) 24 (5.0) 28 (4.3) HHF*2 ... P* HHD/X (No HHE or HHF*2) 10 9 -0 .16 ± 0.09 0.560 37 0 .24 ± 0 .16 0 .11 8 HHE/X (No HHD or HHF*2) 69 0.36 ± 0 .11 0.002 31 0 .10 ± 0 .16 0.556 HHD/HHE 18 -0 .16 ± 0.20 0.444 16 0.54 ± 0.22 0. 014 HHF*2/HHF*2 ... 0.5 31 HHD 16 7 1. 0 0.74 -1. 32 0.983 94 0.9 0.59 -1. 23 0.385 73 1. 2 0.75 -1. 92 0.442 HHE HHD/HHE 15 8 31 1 .1 1.9 0.82 -1. 51 1 .14 -3 .16 0.495 0. 015 85 19 1. 3 2.0 0.86 -1. 87 1. 08-3.57 0.234 0.028 73 12 0.9 1. 7...
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Báo cáo y học: "Conformational alterations in the CD4 binding cavity of HIV-1 gp120 influencing gp120-CD4 interactions and fusogenicity of HIV-1 envelopes derived from brain and other tissues" pptx
... regions include the a-helices of the inner domain, the CD4 binding loop of outer domain, and the b20-b 21 ribbon which becomes part of the gp120 bridging sheet, which is a structural element of gp120 ... binding that is involved in coreceptor binding [3 ,11 ] Changes in CD4 binding to gp120 contribute to different pathophysiological phenotypes of HIV- 1, including the fusogenic properties of the ... alterations in the CD4 binding cavity of HIV- 1 gp120 influencing gp120-CD4 interactions and fusogenicity of HIV- 1 envelopes derived from brain and other tissues Retrovirology 2 011 8:42 Submit...
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Báo cáo y học: "Human defensins 5 and 6 enhance HIV-1 infectivity through promoting HIV attachment" ppsx
... a dominant role in the killing of Gram-positive bacteria, inhibition of anthrax lethal factor, and binding of HIV gp120 by HNP -1 [44] While other defensins such as HNP -1, HNP-4, and HBD3 interact ... alpha-defensins, HD6 exhibits little antibacterial activity [36] HNPs1-4 inhibit HIV infection [15 ,37], whereas HD5 and HD6 promote HIV infection [17 ] Although both HD5 and HD6 are Paneth cell defensins, ... Results Pre-incubation of HIV with defensins significantly increased HIV infection We have previously shown that HD5 and HD6 increase HIV infection when HIV is pre-treated with defensins [17 ] Additionally,...
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Báo cáo y học: " APOBEC3G-UBA2 fusion as a potential strategy for stable expression of APOBEC3G and inhibition of HIV-1 replication" pot
... Trends Biochem Sci 19 96, 21( 5) :17 2 -17 3 Elder RT, Song XQ, Chen M, Hopkins KM, Lieberman HB, Zhao Y: Involvement of rhp23, a Schizosaccharomyces pombe homolog of the human HHR23A and Saccharomyces ... F, Hance AJ: Hypermutation of HIV- 1 DNA in the absence of the Vif protein Science 2003, 300(5622) :11 12 Zhang H, Yang B, Pomerantz RJ, Zhang C, Arunachalam SC, Gao L: The cytidine deaminase CEM15 ... References 10 Goila-Gaur R, Strebel K: HIV- 1 Vif, APOBEC, and intrinsic immunity Retrovirology 2008, 5: 51 Sheehy AM, Gaddis NC, Choi JD, Malim MH: Isolation of a human gene that inhibits HIV- 1 infection...
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Báo cáo y học: "Selective translational repression of HIV-1 RNA by Sam68DeltaC occurs by altering PABP1 binding to unspliced viral RNA" pps
... cellular RNA binding proteins in regulation of HIV- 1 alternative RNA splicing Curr HIV Res 2006, 4 (1) :43-55 Hope TJ: The ins and outs of HIV Rev Arch Biochem Biophys 19 99, 365 :18 6 -19 1 Pollard V, ... or more of these steps This is essential for the production of the HIV1 structural proteins and therefore, the HIV- 1 lifecycle The HIV- 1 env reporter, pgTat, expresses gp160 /12 0 from the unspliced, ... implicated in RNA binding, the findings suggest that these regions might be involved in mediating protein-protein interactions Interestingly, the requirements for inhibition appear distinct from those...
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Báo cáo y học: "Destabilization of the TAR hairpin leads to extension of the polyA hairpin and inhibition of HIV-1 polyadenylation" pptx
... ++ Replication ++ ++ - - ++ A B AB Δ39-48 11 -24 Δ39-48 TARwt HIV- LAI TARm HIV- rtTA 11 -24 Figure The HIV- rtTA genome and mutations in the TAR hairpin The HIV- rtTA genome and mutations in the ... 36:4352-4363 Huthoff H, Berkhout B: Two alternating structures for the HIV1 leader RNA RNA 20 01, 7 :14 3 -15 7 Abbink TEM, Ooms M, Haasnoot PCJ, Berkhout B: The HIV- 1 leader RNA conformational switch regulates ... Average values obtained in three transfections are shown, with the error bars indicating the standard deviation (B) HeLa X1/6 cells were transfected with the HIV- rtTA variants and the intracellular...
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Báo cáo y học: "Characterization of the HIV-1 RNA associated proteome identifies Matrin 3 as a nuclear cofactor of Rev function" ppsx
... in HIV RNA binding/regulation EEF1A1 Involved in RNA-dependent binding of Gag 19 15 [ 81] ILF3 NF90 binds HIV- 1 TAR and RRE (*) 3609 [ 51, 52] MOV10 RNA helicase that inhibits HIV- 1 replication 4343 ... identify novel host factors associated with Page of 15 HIV- 1 RNA To this end we took advantage of two HIV- 1 derived vectors called HIV_ Exo _24 × MS2 (HIVexo) and HIV_ Intro _24 × MS2 (HIVintro), described ... mRNA binding to ribosome 19 73 Other HIST 1H1 A histone 1, H1 a 3 024 H1 FX histone family, H1 member X 89 71 PRKDC DNA-dependent protein kinase (DNA-PKcs) involved in dsDNA break repair 55 91 RIF1 Associated...
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