cell cycle transcription and translation
Báo cáo khoa học: The influence of cold shock proteins on transcription and translation studied in cell-free model systems pdf
... onthe transcriptional or on the translational level, thesystem of combined transcription ⁄ translation wasdecoupled. The influence on the transcriptional levelwas investigated by in vitro transcription ... maximize the cell- free H-Ras expression by reducingInfluence of CSPs on transcription and translation R. Hofweber et al.4692 FEBS Journal 272 (2005) 4691–4702 ª 2005 FEBS translation ⁄ transcription ... proteins on transcription and translation studied in cell- free model systemsRoland Hofweber1, Gudrun Horn1, Thomas Langmann2, Jochen Balbach3, Werner Kremer1,Gerd Schmitz2 and Hans...
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Modeling Cell Cycle Control and Cancer with pRB Tumor Suppressor
... mechanismsfor controlling cell cycle progression (Morgan 1997; Zachariae and Nasmyth1999). These studies and others demonstrated the universality of the cell Modeling Cell Cycle Control and Cancer with ... Nature 343:665–669Modeling Cell Cycle Control and Cancer with pRB Tumor Suppressor 231 cycle, and strongly suggested that deregulation of the mechanisms controlling cell cycle progression could ... Orc1 and Mcm2–7) and DNA replication (e.g.Dhfr, Rnr, TK, TS and Polα). Many E2F target genes encode products in-volved in cell cycle progression (e.g. cyclins A and E, pRB, p107, E2F1–3) and apoptosis...
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The Retinoblastoma Gene Family in Cell Cycle Regulation and Suppression of Tumorigenesis
... most cell cycle responses. While each single pocket protein can mediate cell cycle arrest upon cell- cell contact, pRb is the critical mediator of cell cycle arrestupon growth factor deprivation and ... roles in controlling cell cycle exit upon growth-inhibiting signals. Only ablation of all pocket proteins fullyalleviated a cell cycle arrest upon serum withdrawal, cell- cell contact inhi-bition, ... suppression and cell mortality. Cell 85:27–37Serrano M, Lin AW, Mila EM, Beach D, Lowe SW (1997) Oncogenic ras provokes prema-ture cell senescence associated with accumulation of p53 and p16Ink4a. Cell...
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Báo cáo khoa học: 3 Cdt1 and geminin are down-regulated upon cell cycle exit and are over-expressed in cancer-derived cell lines potx
... during the cell cycle [13], upon exit and entry tothe cell cycle both genes are controlled, at least in part,transcriptionally.Cdt1 and geminin are highly expressed in proliferatingcellsin ... upon cell cycle exit. To this effect, HFF cells weredeprived of serum f or 48 h to induce cell cycle exit. Serumwas th en r e-added a nd samples taken as cells progressedinto the cell cycle. ... in G0 cells and induced upon cell cycle re-entry[23] and to be under the transcriptional c ontrol of E2F [24–26]. We show here that, similar to Cdc6, Cdt1 mRNA levelsare r educed in G0 and r...
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cell cycle checkpoints and cancer
... the ligand for the flt2/3 receptor), erythropoietin (EPO), and others affect the growth and differentiation of these early hematopoietic precursor cells intocells of the myeloid, lymphoid, and ... knowledge of how cytokines affect cell growth, signaltransduction, cell cycle progression, and apoptosis has been elucidated from research with IL-3 and IL-3-dependent cell lines. IL-3 was initially ... Schrader JW, Clark-Lewis I. A T cell- derived factor stimulating multipotential hemopoietic stemcells: molecular weight and distinction from T cell growth factor and T cell- derivedgranulocyte-macrophage...
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Senescence and Cell Cycle Control
... RA (1995) The role of Rb in cell cycle control. Prog Cell Cycle Res 1:9–19Senescence and Cell Cycle Control 259eliminates telomere-mediated signaling, allowing cells to undergo transform-ation ... loss of G1 control and immortalization. Genes Dev 14:3037–3050Senescence and Cell Cycle Control 267Campisi J (2001) Cellular senescence as a tumor-suppressor mechanism. Trends Cell Biol11:S27–S31Carnero ... mouse cells. p19ARFispredominantly upregulated during senescence by oncogene activation and/ or oxidativestressSenescence and Cell Cycle Control 263induction by controlling p16INK4a(and...
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Tài liệu Báo cáo khoa học: The central role of CDE/CHR promoter elements in the regulation of cell cycle-dependent gene transcription pdf
... RegulatedDuring the Cell Cycle by a CDE ⁄ CHR TandemElement and is Downregulated by p53 but not by p63or p73. Cell Cycle 6, 853–862.37 Yoshida K (2005) Cell- cycle- dependent regulation ofthe human and mouse ... p53. Cell cycle arrest can be medi-ated by p53 downregulating the transcription of central cell cycle regulators such as cyclin B, Cks1, Cdc2 and Cdc25C.CDE ⁄ CHR-dependent cell cycle- gene transcription ... encoded by cyclin B1 and cyclin B2 genes isKeywords cell cycle; cell cycle genes homology region(CHR); cell cycle- dependent element (CDE);DREAM complex; E2FCorrespondenceK. Engeland, Molecular...
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Tài liệu Báo cáo khoa học: Protein kinase CK2 activates the atypical Rio1p kinase and promotes its cell-cycle phase-dependent degradation in yeast pdf
... phase of the cell- division cycle. Therefore, we tested,using light microscopy and after DAPI staining, whetherthe slow growth yields a cell- cycle phenotype. Cells ofthe logarithmically ... which is stable throughout the cell cycle and highly resistant to proteolytic degradation [40].Miscellaneous proceduresYeast were grown on standard media [41] and transformedas described by ... distribution of G2⁄ M cells: thenumber of metaphase cells with a single DNA mass atthe bud neck and the number of anaphase cells wereincreased slightly in the mutant (metaphase cells: 30.2%in the...
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Báo cáo khoa học: Injection of poly(b-L-malate) into the plasmodium of Physarum polycephalum shortens the cell cycle and increases the growth rate pot
... in growth and cell cycle duration. However, wedo not know how great an effect should be expected. Ifwe assume that certain PMLA–protein complexes areinvolved in growth and cell cycle timing, ... proteins tonucleic acids, and it c ould affect cell g rowth a nd cell cycle duration.The distributing activity of PMLA and the efficiency ofcompetition between PMLA and nucleic acids would both ... PMLA induced a strain specific enhancement of cell growth and an equal (between strains) shortening of the cell cycle. Materials and methodsStrains and materialsThe following strains of P. polycephalum...
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Báo cáo khoa học: "Braille Transcription and Mechanical Translation" pptx
... r"occupies three cells. In the second case the translation occupies three cells since "er" is a single -cell contraction. By rule 35 "o n er" is the correct translation of ... construction of the whole program and, in particular, of the dictionary. [Mechanical Translation , vol.2, no.3, December 1955; pp.50-53] Braille Transcription and Mechanical Translation John P. ... the dictionary and precedes "one". "Oner" may be contracted in two ways - "one r" and "o n er. "In the first case "one" is a two -cell contraction...
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Báo cáo Y học: Synthesis and turn-over of the replicative Cdc6 protein during the HeLa cell cycle potx
... wasremoved, and cells were washed several times with normalmedium and then grown un der standard conditions.For proteasome inhibition, HeLa cells were synchronizedby a double t hymidine-block and ... during the HeLa cycle (Eur. J. Biochem. 269) 1041Synthesis and turn-over of the replicative Cdc6 proteinduring the HeLa cell cycle Esther Biermann, Martina Baack, Sandra Kreitz and Rolf KnippersDepartment ... characteristic for the yeast cell cycle does notoccur i n mammalian cells, and levels of human Cdc6p(hCdc6p) in cycling human cells remain fairly stable duringS phase, G2 phase and mitosis [24–27],...
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Báo cáo khoa học: Dynamic association of MLL1, H3K4 trimethylation with chromatin and Hox gene expression during the cell cycle ppt
... the cell cycle; rather, old marks are removed and new marks are introduced. In conclusion, our studies demonstratethat MLL1 and H3K4 methylation have distinct dynamics during the cell cycle and ... the cell cycle As MLL1 and H3K4 trimethylation show distinctdynamics during cell cycle progression, we analyzedthe expression profiles of MLL1, CGBP, Ash2 and Rbbp5, together with cyclins E and ... function ofthe cell cycle. Western blot analysis of the whole -cell extract and histones from different stages of the cell cycle demonstrated that the overall levels of MLL1 and H3K4 trimethylation...
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