calcium calmodulin dependent protein kinase ii camkii a major signaling molecule and scaffold of the psd
Báo cáo khóa học: Phospholipase C, protein kinase C, Ca 2+ /calmodulin-dependent protein kinase II, and redox state are involved in epigallocatechin gallate-induced phospholipase D activation in human astroglioma cells ppt
... via a signaling pathway involving redox -dependent changes in the cell, which stimulate the PLC-c1 [Ins(1,4,5)P3–Ca2+]–Ca2+ /calmodulin- dependent protein kinase II (CaM kinase II) –PLD pathway and ... PKC activation EGCG treatment stimulated PKC -a translocation to the plasma membrane, and it appears that all of the enzyme associates with the membrane on stimulation with EGCG (Fig 9C) This translocation ... phosphorylation of PLC-c1 which was inhibited by pretreatment of antioxidant (data not shown) The effects of EGCG on the activation of PLC and PLD are reversed by N-acetylcysteine and catalase, suggesting...
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Báo cáo Y học: Autophosphorylation-dependent inactivation of plant chimeric calcium/calmodulin-dependent protein kinase docx
... in anthers Planta 209, 161–171 11 Takezawa, D., Ramachandiran, S., Paranjape, V & Poovaiah, B.W (1996) Dual regulation of a chimeric plant serine/threonine kinase by calcium and calcium/ calmodulin ... remove any impurities CCaMK was autophosphorylated as described above and the reaction was terminated by the addition of EDTA (to a final concentration of 50 mM) and the sample was kept in ice The autophosphorylated ... modulating the Ca2+/CaM mediated signal transduction pathway in anther The elucidation of the molecular mechanisms leading to the self-inactivation of CCaMK will broaden our understanding of the...
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báo cáo khoa học: " A rice calcium-dependent protein kinase is expressed in cortical root cells during the presymbiotic phase of the arbuscular mycorrhizal symbiosis" pps
... 5’-CGGGGATCCATGAGTAAAGGAGAAGAACTTTTCAC-3’ (forward) and 5’CCGAGCTCTTATTATTTGTATAGTTCATCCATGC3’ (reverse) During the PCR reaction, BamHI and SacI sites were introduced into the PCR amplified DNA fragment ... conformational changes that activate their kinase activity Page of 14 (calcium- bound structure) [51] It is then reasonable to assume that the plasma membrane-localized OsCPK18 protein sense the AM-induced ... Alignment of the amino acid sequences of plant CPKs and CCaMKs Amino acid sequences from rice (Os), wheat (Ta), maize (Zm) and Medicago (M truncatula, Mt; M sativa, Ms) were aligned Black and gray backgrounds...
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Báo cáo khoa học: The hinge region operates as a stability switch in cGMP-dependent protein kinase Ia doc
... Crystal structures of a similar enzyme from the AGC-family of protein kinases, cAMP -dependent protein kinase (PKA) have greatly contributed to our understanding of PKG’s intra- and inter-domain ... times (1 h), revealed no major other cleavage products (data not shown) Addition of cGMP had a remarkable effect on the stability of the R77L mutant Now, a rather rapid degradation was observed (Fig ... Da), as depicted in the inset of Fig These N-terminal fragments all confirmed the abovestated N-terminal acetylation and elimination of the first methionine amino acid At the retention time of the...
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Báo cáo khoa học: HIP/PAP, a C-type lectin overexpressed in hepatocellular carcinoma, binds the RIIa regulatory subunit of cAMP-dependent protein kinase and alters the cAMP-dependent protein kinase signalling ppt
... subcellular compartment The locations of HIP/PAP and RIIa were further analyzed using a fractionation method (Fig 1B) The regulatory RIIa and the catalytic Ca subunits of PKA were detected in the ... heart PKA The sense primer (5¢-GTCGAATTCCAAGGTG AAGAACCCCAG-3¢) was located at nucleotides 63–90 of the coding sequence, and the antisense primer (5¢-TG CTGAATTCCCTCCCTCCTGCACTAGTCAG-3¢) overlapped ... Hepatocarcinoma-intestine-pancreas/pancreatic associated protein (HIP/PAP) is expressed and secreted by proliferating ductules as well as by hepatocarcinoma and cholangiocarcinoma cells Am J Pathol...
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Tài liệu Báo cáo khoa học: Aggregative organization enhances the DNA end-joining process that is mediated by DNA-dependent protein kinase pdf
... separate the aggregate and the supernatant (sup.) after h indicated that both input substrates and the EJ products remained stable in the aggregates (compare lanes and and lanes and 6) Fig S2 The ... Aggregation-coupled DNA end-joining M Takahagi and K Tatsumi One hallmark of the initial response to DSBs is the rapid activation of ATM (product of the ataxia telangiectasia mutated gene), the ... marker proteins are 94, 67, 43, 30 and 20 k in the context of an alternative EJ pathway [25,26] The characterization of ligase III is underway in the aggregation system The aggregative nucleoproteins...
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Báo cáo khoa học: Serine-arginine protein kinases: a small protein kinase family with a large cellular presence potx
... role(s) of each one of the SRPK family members and the significance of the ‘spacer domain’ for the functional properties and the particular regulation of each kinase l The extra- and intracellular ... SAFB molecules impaired the catalytic activity of SRPK1 ⁄ 1a, whereas the nuclear subfraction of the kinases, which was found to be associated with the nuclear matrix via SAFB proteins, was inactive ... domain l The exact share the SRPKs hold amongst the other kinases that also recognize RS dipeptides, such as the Clk and Akt family of kinases, as well as the cross-regulation between them l The...
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Báo cáo khoa học: Identification of the structural determinant responsible for the phosphorylation of G-protein activated potassium channel 1 by cAMP-dependent protein kinase pdf
... may be located distal to amino acid 411 Functional aspects of mutation of S385, S401 and T407 The effects of PKA-catalysed phosphorylation on rat atrial IK+ACh as well as on basal and agonist induced ... GIRK1 (the cAMP effect was assessed as IcAMP ⁄ IHK of a given oocyte) Data are the mean ± SEM The number of experiments is given in parenthesis above each bar The mean value differs statistically ... 2009 The Authors Journal compilation ª 2009 FEBS C Mullner et al ¨ PKA phosphorylation of GIRK1 the Austrian Research Foundation (SFB708) and the Research Foundation of the Austrian National Bank...
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Báo cáo khoa học: Identification of multiple isoforms of the cAMP-dependent protein kinase catalytic subunit in the bivalve mollusc Mytilus galloprovincialis potx
... to the PAM C-subunit The application of a salt gradient resulted in separation of four absorbance peaks Three of them – labelled peak I, peak II and peak III – showed protein kinase activity; they ... a particular region of the proteins On the other hand, the presence of a peak at 1605.8 Da was observed in the spectra of C2, C3 ⁄ C5 and C6 that was absent in those of C1 and C4; moreover, a ... Two absorbance peaks, associated with protein kinase activity, were separated; these eluted at 0.19 and 0.25 m NaCl, and were labelled peak I and peak II, respectively Coomassie staining of an...
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Báo cáo khoa học: Purification and structural study of the b form of human cAMP-dependent protein kinase inhibitor pdf
... Table According to the activity assay, the activity was retained above 95% (data not shown) and the recovery is 24% during the heat-treatment It implied that PKIb was a thermostable protein and ... Fig The 1652 and 1270 cm)1 bands can be assigned to a- helix in the human PKIb; the 1666 and 1247 cm)1 bands are the characteristic frequencies of the random coil structure Meanwhile, the 1682 and ... Kumar, P., Van Patten, S.M & Walsh, D .A (1997) Specific testicular cellular localization and hormonal regulation of the PKI and PKI isoforms of the inhibitor protein of the cAMP -dependent protein...
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Báo cáo khoa học: In vitro embryonic developmental phosphorylation of the cellular nucleic acid binding protein by cAMP-dependent protein kinase, and its relevance for biochemical activities pdf
... (5¢-CCTTTTCTCTTCGTGGCCGCTGTGGAG AAGCAGCGAGGAGATG-3¢), and Comp-CT representing the complementary (antisense) strand of the CT element from the human c-myc promoter (see below) Strand annealing assays Oligonucleotide annealing ... 12 Yasuda J, Mashiyama S, Makino R, Ohyama S, Sekiya T & Hayashi K (1995) Cloning and characterization of rat cellular nucleic acid binding protein (CNBP) cDNA DNA Res 2, 45–49 13 Tomonaga T, ... test) protein that binds single-stranded nucleic acids and is able to promote interstrand reannealing of DNA as well as RNA [35,36] hnRNP Al is phosphorylated by CK2 and PKA PKA phosphorylation...
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Báo cáo khoa học: Double-stranded RNA-dependent protein kinase (PKR) is downregulated by phorbol ester ppt
... factor-alpha, and doublestranded RNA Blood 97, 1644–1652 Zamanian-Daryoush M, Mogensen TH, DiDonato JA & Williams BR (2000) NF-kappaB activation by double-stranded-RNA-activated protein kinase (PKR) ... mediated through NF-kappaB-inducing kinase and IkappaB kinase Mol Cell Biol 20, 1278–1290 Ishii T, Kwon H, Hiscott J, Mosialos G & Koromilas AE (2001) Activation of the IkappaB alpha kinase (IKK) ... Natl Acad Sci USA 94, 3279–3283 Balachandran S, Kim CN, Yeh WC, Mak TW, Bhalla K & Barber GN (1998) Activation of the dsRNAdependent protein kinase, PKR, induces apoptosis through FADD-mediated...
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Báo cáo khoa học: Structured DNA promotes phosphorylation of p53 by DNA-dependent protein kinase at serine 9 and threonine 18 doc
... rather than a minor contaminant of the DNA-PK preparation Although our SDS/PAGE analysis indicated that the DNA-PK was about 90% pure, the potential contribution of contaminating kinases had to ... samples and kinasing of p53wt (lanes 1, 2) or p53S1 5A/ S3 7A (lanes 3, 4) was performed by standard assay On the left is a Phosphorimager analysis of a representative gel and on the right is a ... eluted in H2O An aliquot was then evaporated to dryness and 5.5 M HCl was added for h at 110 °C The hydrolysate was evaporated, mixed with unlabeled pSer, pThr and pTyr standards and then applied onto...
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Báo cáo khoa học: " A specific inhibitor of protein kinase CK2 delays gamma-H2Ax foci removal and reduces clonogenic survival of irradiated mammalian cells" pot
... treatment condition) before calculating the displayed mean values (and standard deviations) from at least independent determinations localized and transient changes of chromatin organization that ... informatics and support with statistics for data analysis KW conceived of the study, participated in its design and helped to draft the manuscript All authors read and approved the final manuscript ... doi:10.1186/1748-717X-6-15 Cite this article as: Zwicker et al.: A specific inhibitor of protein kinase CK2 delays gamma-H2Ax foci removal and reduces clonogenic survival of irradiated mammalian cells Radiation Oncology...
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Báo cáo y học: "Activation of HIV-1 expression and replication by cGMP dependent protein kinase type 1-β (PKG1β)" docx
... interdigitate with some of the signaling routes utilized by HIV-1 in infected cells [22] Additionally, because many cGMP chemical agonists and antagonists are available [23,24], practical chemotherapeutic ... part by intramural funding from NIAID, NIH; and by the intramural AIDS targeted antiviral program (IATAP) from the Office of the Director, NIH We thank Dr S.M Lohmann for PKG1β expression plasmid ... and transfected as indicated with HIV-1 LTR luciferase reporter plasmid in the absence and presence of Tat, and with a PKG1-β expression plasmids PKG1β was found to increase Tat dependent transcriptional...
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THE INFLUENCE OF THE KU80 CARBOXY-TERMINUS ON ACTIVATION OF THE DNA-DEPENDENT PROTEIN KINASE AND DNA REPAIR IS DEPENDENT ON THE STRUCTURE OF DNA COFACTORS Derek S.
... GAG ATG CTG ATG ATA AAC ACA AGC TTA TAA CCA AAA CAG AGG CAA AAC AAG AAT ATC TTC TGA AAG ACT GTG ATT TAG AAA AAA GAG AGC CAC CTC TTA AAT TTA TTG TGA AGA AGA ATC CAC ATC ATT CAC AAT GGG GTG ATA ... TTA TAA CCA AAA CAG AGG CAA AAC AAG AAT ATC TTC TGA AAG ACT GTG ATT TAG AAA AAA GAG AGC CAC CTC TTA AAT TTA TTG TGA AGA AGA ATC CAC ATC ATT CAC AAT GGG GTG ATA TGA AAC TCT ACT TAA AGT TAC AGA ... TGA AAC TCT ACT TAA AGT TAC AGA TTG TGA AGA GGT CTC TTG AAG TTT GGG GTA GTC AAG AAG CAT TAG AAG AAG CAA AGG AAG TCC GAC AGG AAA ACC GAG AAA AAA TGA AAC AGA AGA AAT TTT AGT AGG GAT CCG TGG ATT...
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Identification and characterization of protein kinase CK2 as a novel interacting protein of neuronal CDK5 kinase and its functional role in microtubule dynamics
... a. a amino acid AD Alzheimer’s disease APP amyloid precursor protein ATP adenosine triphosphate c-abl c-Abelson CAK Cdk activating kinase CaMKII Ca2+ /calmodulin- dependent protein kinase II cAMP ... phosphorylating protein phosphatase 2A (PP 2A) and thence by stimulating its activity, could indirectly cause down-regulation of the PP 2A substrate MEK and thus block the activation of the Raf-MEK-MAPK ... α-actinin and the α-subunit of Ca2+ /calmodulin- dependent protein kinase II (CaMKII ) were identified as p3 5and p39-interacting proteins (Dhavan et al., 2002) Either of these two proteins forms a complex...
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Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx
... neurons A previous in vitro study indicated that the RasGEF activity of v-KIND induces the phosphorylation of MAP2 by JNK1 and ⁄ or ERK via the activation of the Ras–Raf–MAP kinase pathway [12] These ... regulates the dendrite arborization patterns that are critical for shaping neuronal circuits, and also may provide a clue to the understanding of some MAP2-associated neurodegenerative and psychiatric ... signal transduction pathways [1,2] The kinase noncatalytic C-lobe domain (KIND) was determined to be a putative signaling domain based on bioinformatic analysis of the N-terminal sequence of the...
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Tài liệu Báo cáo Y học: Identification and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx
... (5¢-CGCGGATCCATGTATAAGACAATCATTATGC-3¢) containing a BamHI site (underlined nucleotides) and a 3¢ deoxyoligonucleotide harboring a HindIII site (5¢-CCCAA GCTTTTAACGCACAACCAGCACC-3¢) as primers ... exponential growth; it starts to accumulate at the early stationary phase, and its Ó FEBS 2002 steady-state level increases further during the late stationary phase As a result of this accumulation, the ... estimate the ratio between the amounts of UP12 and the total amount of protein in the extracts, a series of samples containing determined amounts of the purified UP12 were separated by SDS/PAGE along...
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Báo cáo khoa học: Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL family docx
... consists of three domains The largest domain encompasses strands b1 to b6 and strand b7 to the C-terminus The central b sheet is composed of parallel b strands associated to an antiparallel strand ... minima at 205 and 215 nm, indicating that Rv1399c is composed of 22% of a- helices, 25% of b-strand and 39% of random coil The catalytic triad is located at the bottom of a small ˚ ˚ pocket, 20 A ... functional catalytic triad made of a catalytic nucleophile serine, associated to a proton carrier histidine and a charge relaying aspartic (or glutamic) acid To further investigate the biochemical...
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