aureus identification for the nuc or fema gene and detection of the meca for methicillin resistance
Cloning genes by PCR
... only one strand of the vectorette adaptor and therefore can only copy DNA after the first cycle of PCR when the GSP1 copies the vectorette adaptor strand to yield a sequence to which the AP1 can ... essence the procedure is identical to the generation of a library by ligation of a double-strand adaptor The adaptor is added to the 5′- and 3′-ends of each molecule in the library whether derived ... endonucleases and accompanying buffer Phenol-chloroform mix (e.g phenol:chloroform:iso-amyl alcohol 25:24:1, GIBCO-BRL) T4-DNA ligase and accompanying buffer Gene- specific primers and nested gene- specific...
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Báo cáo khoa học: The lactate dehydrogenases encoded by the ldh and ldhB genes in Lactococcus lactis exhibit distinct regulation and catalytic properties ) comparative modeling to probe the molecular basis pdf
... binding of Fru(1,6)P2 to allosteric lactate dehydrogenases is connected with the conversion between the inactive T form and the active R form of the tetramer [23]; the affinity of LDHB for Fru(1,6)P2 ... build-up of NADH, which was not expected, as the levels of cofactor should be sufficient to sustain the lactate flux Therefore, there must be an additional factor acting as an inhibitor of LDHB, and the ... [29] Therefore, we measured the activities of PFK and PK in strain FI9078, and found that they were reduced to 48% and 66% of the MG1363 levels Although the activity of these enzymes, i.e 0.48 and...
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Genes and Autoimmunity - Intracellular Signaling and Microbiome Contribution by Spaska Angelova Stanilova pot
... intravenous glucocorticoid therapy and /or irradiation of the retro-orbital lesion Furthermore, there are also other symptoms of GO including inflammation of the lachrymal glands and /or eyelids and eyelid ... expressed in the chapters are these of the individual contributors and not necessarily those of the editors or publisher No responsibility is accepted for the accuracy of information contained in the ... 21 for CC, 15 for CT, and1 6 for TT) and in 48 SLE patients (n = 17 for CC, 24 for CT, and for TT) As the mean TGF-β1 levels varied significantly by case-control status (P < 0.001), patients and...
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Báo cáo khoa học: "Isolation and identification of Escherichia coli O157:H7 using different detection methods and molecular determination by multiplex PCR and RAPD" docx
... centrifugation for The supernatant was collected and used as a template Multiplex PCR for stx1, stx2, eaeA, and hlyA, and the flagellar H7 gene amplification Multiplex PCR for the detection of stx1, ... for 18 h at 37oC Inhibitory zones of the growth were measured The results were interpreted by the guideline of National Committee for Clinical Laboratory Standards (NCCLS) Results Isolation of ... risk of spread of major foodborne pathogens Acknowledgments This study was supported by the Rural Development Administration, the Ministry of Agriculture and Forest, and also supported by the...
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Báo cáo khoa học: " Identification and prevalence of Ehrlichia chaffeensis infection in Haemaphysalis longicornis ticks from Korea by PCR, sequencing and phylogenetic analysis based on 16S rRNA gene" docx
... 94 C for min, annealing at 60 C for min, extension at 72 C for 30 sec, and one cycle of extension at 72 C for For second round nested-PCR, pmol of each primer, HE1 and HE3 (Genotech, Korea) and ... (AF416764), the Sapulpa (U60476) and the 91HE17 (U23503) strains of E chaffeensis, all of these originate from the USA We also performed phylogenetic analysis of EC-PGHL strain in order to determine the ... ticks in Korea 153 PGHL was 100% identical or similar to the Arkansas (AF416764), the Sapulpa (U60476) and the 91HE17 (U23503) strains, all of these originate from the USA (Table 2) The phylogenetic...
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Báo cáo y học: "Analysis of bacterial DNA in synovial tissue of Tunisian patients with reactive and undifferentiated arthritis by broad-range PCR, cloning and sequencing" pps
... 12 of 14 (page number not for citation purposes) Authors' contributions MS performed the experimental work, analyzed the data and wrote the manuscript RG conceived of the study, performed the ... question about the role that this variety of intraarticular bacterial DNA plays in the pathogenesis of ReA and other forms of arthritides However, detection of bacterial DNAs in the ST of patients ... tuberculosis, or Borrelia burgdorferi) in synovial fluid or synovial tissue (ST) from patients with ReA or other forms of arthritis (UA, RA, or OA) has raised the question of whether non-Chlamydia or nonenteric...
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Báo cáo y học: "Tetra-O-methyl nordihydroguaiaretic acid (Terameprocol) inhibits the NF-B-dependent transcription of TNF-a and MCP-1/CCL2 genes by preventing RelA from binding its cognate sites on DNA" ppsx
... modifications to the backbone structure of NDGA [11] A number of these compounds have been examined for their antiviral activity For example, an analysis of eight methylated forms of NDGA for their ability ... RelA The range of inhibitory effects seen with TMP with different cytokines and chemokines may arise from the differential requirements of these genes for the various Page of 11 modified forms of ... from the National Institutes of Health The authors wish to thank D Eads and K Belanger-Crook for their technical assistance Authors’ contributions AOO performed the experiments and drafted the...
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Báo cáo khoa học: " Detection and identification by PCR of Clostridium chauvoei in clinical isolates, bovine faeces and substrates from biogas plant" potx
... part of the substrate for biogas plants, and spore-forming bacteria may survive pasteurisation and digestion [7,8] An anaerobic digestion process is commonly used for energy production in the form ... responsible for the PCR and sequence methods, and discussed and approved the final manuscript Acknowledgements The authors would like to thank: Detection threshold of the PCR method The detection ... septicum and C perfringens were not detected by the specific primer pair used for detecting of C chauvoei Detection threshold of the PCR method The detection thresholds for samples consisting of manure,...
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Báo cáo y học: "Wound healing and inflammation genes revealed by array analysis of ''''macrophageless'''' PU.1 null mice" ppt
... Heatmaps for activation and effector clusters Heatmaps for activation and effector clusters Color depiction of the temporal profiles of mRNA intensity during the 24 h repair period for genes in (a) the ... Temporal and spatial expression profiles of sample genes from the activation and effector clusters Temporal and spatial expression profiles of sample genes from the activation and effector clusters ... activation genes The profiles of these two clusters are temporally distinct from one another and so we have called them the early effector and late effector clusters Between them they contain 184 genes...
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Generation of mouse graves ophthalmopathy model with full length TSH receptor plasmid and cytokine evaluation by real time PCR
... useful for differential diagnosis of GD from other causes of hyperthyroidism, for follow up of patients with GD under treatment with antithyroid drugs, for the diagnosis of GO and for monitoring ... thank Prof Donald Tan for supporting and allowing me to pursue a Master’s degree and Dr Daphne Khoo for those enjoyable years of working under her supervision and for inspiring and supporting ... are heterogeneous in their genetic makeup A complex network of genetic factors 17 governs the response of the immune system Genetic factors such as HLA, T cell regulatory gene, polymorphisms in...
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ESTROGENIC ACTIVITY LEVEL OF NAKDONG RIVER BASIN AND ITS CONTROL BY WATER TREATMENT PROCESSES
... Rapid and Comprehensive indicator for evaluating the performance of treatment processes, Proceeding of 2002 IWA World Water Congress, e20217a Kamata M., Evaluation of estrogenic activity of natural ... estrogenic activity level by the combination of sand filtration and ozonation processes Fig indicates the behavior of estrogenic activity for each purifying treatment process For water-purifying processes, ... over the whole middle and downstream excluding the upstream of Andong Relative estrogenic activity of estrogenic substances in the Sewage treatment plants and Water treatment plants The removal of...
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Moment and Shear, Generated by DL on the Composite Section
... The effect of the design tandem combined with the effect of the design lane Combination 2: The effect of the design truck combined with the effect of the design lane Combination 3: For both the ... points of contraflexure under a uniform load on all spans, and reaction at interior piers only 90% of two design trucks spaced a minimum of 50 ft between the lead axle of truck and the rear axle of ... strands 12 12 10 Harped "y" or "n" n n n 2 n n LRFD pre-stressed beam.mcd 7/1/2003 29 of 71 End Pattern 60 Do not worry about if the "x" coordinate of a strand or a strand break is not correct The...
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a discourse analysis of opening and closing speeches by masters of ceremonyon reality television showsin american english versus vietnames
... short form of the word” [24] “He’s” may be a contraction of “he has” or “he is” They can be formal like “I’m” for “I am” or informal like “gonna” for “going to” Slangs are “very informal words ... containing the content of the show in which the MC explains the rules of the shows Therefore, the terms of reality TV subjects are frequently used to explain the rules, the performances or how the shows ... of the producer of the message and its receiver, the number of addressees and factors such as public or private occasion are the most important features influencing the selection of either formal...
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A RESEARCH ON ENGLISH INVERSION AND DIFFICULTIES ENCOUNTERED BY ENGLISH MAJORS OF HAI PHONG PRIVATE UNIVERSITY
... above, the rearrangement of the subject and verb or operator from their normal orders in statements is called inversion So the operator performs an important syntactic function It is normally the ... apart from the major classes of sentences, there are the formulae in the sense that although they may have the appearance of belonging to once of the major classes, they not share the features ... phenomenon in which either a verb or an operator stands before subject It is device used to reduce the length of the sentence and to emphasize the important factor theme in the sentence Inversion...
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A study on imperative and errors made by vietnamese learners
... The negative imperative form of English verbs is formed by the present tense form of the verb “do” followed by the adverb “not” and then the affirmative imperative form For example: Negative Infinitive ... in the imperative mood consist of a single word rather than a phrase of two or more words Also unlike other English verb form, the grammatical subject of verbs in the imperative is always the ... to children and to animals The intonation of an order is important: each word is stressed, and the tone falls at the end of the sentence 23 For example: Sit down now! “Sit”; “down” and “now” are...
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A study on subjuntive and errors made by vietnamese learners
... - They will insist that their colleague be admitted to the association 3.2 Use of the past forms of the subjunctive mood Like the past forms of the subjunctive of the active voice, the past forms ... subjunctive, the form “was” is not used, we just only use the form “were” for all persons And the past perfect subjunctive mood of the passive voice has the form of the past perfect tense of the passive ... Passive forms of subjunctive mood 38 3.1 Use of the present forms of the subjunctive mood 39 3.2 Use of the past forms of the subjunctive mood 40 Chapter III: Some errors of subjunctive...
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Tài liệu How to Win Friends and Influence People by Dale Carnegie docx
... to give them kind words of appreciation that would sing in their memories for years like the music of the morning stars Paul Harvey, in one of his radio broadcasts, "The Rest of the Story," told ... Electrical Engineers and the New York Telephone Company have had this training conducted in their own offices for the benefit of their members and executives The fact that these people, ten or twenty years ... to their mother, waited for them to nurse, and then brought them back to the warmth of the kitchen stove There were six hundred students in State Teachers' College, and Dale Carnegie was one of...
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Tài liệu HealthDoc: Customizing patient information and health education by medical condition and personal characteristics pptx
... is one of the technical goals of this project) And unlike text blocks, the SPL representation contains information sufficient for the customization of form and the ‘repair’ of incohesive or incoherent ... more difficult for the author to use than, say, the more-sophisticated features of a typical word processor It is the author’s job to decide upon the basic elements of the text, the cohesive and ... information and qualia-structure information Co-occurrence information for a lexical item describes other lexical units that collocate with it or for which it is a collocate (e.g., perform and...
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