additional file 2 table s2 for full name and synonyms of gene abbreviations used in the following text
Báo cáo y học: "Response of sinusoidal mouse liver cells to choline-deficient ethionine-supplemented diet" ppsx
... The first line shows the shared sequence of M1- and M2-Pk and the second line shows the different sequence of M1-Pk in exon Primers used for sequencing of RT-PCR-products of cell lines and isolated ... 2A) Both, the elevation of M1-Pk and M2-Pk on RNA level and the increase of M-Pk positive cells point to expansion of sinusoidal cells due to CDE diet Therefore, it was necessary to analyse the ... Page of 11 (primer pair 1; Table 2) or only M2-Pk (primer pair 3; Table 2) or M1-Pk (primer pairs 4, and 6; Table 2) (Figure 2A) The identity of mouse M1-Pk was determined by sequencing of partial...
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Báo cáo sinh học: "Curcumin reduces expression of Bcl-2, leading to apoptosis in daunorubicin-insensitive CD34+ acute myeloid leukemia cell lines and primary sorted CD34+ acute myeloid leukemia cells" pptx
... signaling pathways [21 ], including the mitogen-activated protein kinase [22 ], nuclear factor kappaB [23 ], phosphoinositide-3 kinase/Akt/mammalian target of rapamycin [24 ,25 ], Wnt [26 ], and Notchmediated ... min, and 72 C for min; for Bcl -2, 94°C for 30 s, 62 C for 30 s, 72 C for 10 s; and for b-actin: 94°C for 30 s, 55°C for 30 s, 72 C for Thirty cycles of amplification were used PCR (10 μl) products ... Curcumin( M) 40 60 93 .25 6.47 97.31 80 37.65 19.14 23 .86 62. 33 80. 82 92. 88 72. 29 49.91 38 .27 KG1a Red 76.13 Kasumi-1 27 .68 50.05 84.76 20 .25 7 .25 0.36 15 .22 79.73 92. 74 99.63 7.08 U937 2. 58 Red/green...
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Báo cáo y học: " DNA methylation patterns associate with genetic and gene expression variation in HapMap cell lines" ppt
... resulted in a final set of 22 ,29 0 probes (21 ,28 9 autosomal probes) that were used in all further analyses The 22 ,29 0 probes were nearest to the TSSs of 13 ,23 6 Ensembl genes, of which 12, 901 genes ... studying variation in gene expression using microarrays [20 ,22 ] and RNA sequencing [23 ,24 ], as well as smaller studies of variation in chromatin accessibility and PolII binding [25 ,26 ] Finally, ... quantiles based on the mean Page 10 of 13 gene expression per gene For the eQTL analyses, RNAsequencing data were corrected and normalized exactly as in [24 ] Of the 22 ,683 genes in the original study,...
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báo cáo khoa học: "ShRNA-mediated gene silencing of MTA1 influenced on protein expression of ER alpha, MMP-9, CyclinD1 and invasiveness, proliferation in breast cancer cell lines MDA-MB-231 and MCF-7 in vitro" pptx
... persistently inside the cells These findings have opened a broad new avenue for the analysis of gene function and gene therapy [2, 11] Here, we successfully transfected two shRNAs targeting MTA1 gene into ... China) Protein bands were detected using ECL detection system (Zhongshan Golden Bridge Biotechnology, China), and b-actin staining served as the internal standard for the membranes All of the ... BamH and HindⅢ on 0.8% agarose gel electrophoresis, the band about 3 42 bp was cut off using Xba and BamHⅠ, the band about 408 bp was cut off using Xba and HindⅢ (Figure 1) The results of incision...
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Báo cáo y học: "Biochemical and morphological changes in endothelial cells in response to hypoxic interstitial edema" pdf
... number of intersections of test lines with the boundary profile of the capillaries according to Sc = (2 × I)/Lt, where Lt is the total length of all the test lines of the grid (length of each ... http://respiratory-research.com/content/7/1/7 of test line (d = 0.174 µm), P being the number of points falling in the compartment and I tot being its overall surface boundary profile Volume densities (Vv) of endothelial and epithelial ... nuclear shrinkage [22 ] In line, with these findings, we did not find a significant increase of MDA, suggesting that the level of peroxidation remained unchanged in both models of interstitial edema...
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Báo cáo y học: "Thymic plasmacytoid dendritic cells are susceptible to productive HIV-1 infection and efficiently transfer R5 HIV-1 to thymocytes in vitro" pdf
... antibodies for the immunohistochemistry and participated in the design of the study SRL participated in the design and coordination of the study and revised the manuscript PUC participated in the design ... percentiles, the horizontal line in the box is the median and the whiskers extend to the minimum and maximum data points * indicates a p value of < 0.05 as determined by the Wilcoxon signed rank test The ... blood mDC following infection with both R5 (10 and events/10 viable cells respectively; p = 0 .22 ) and X4 HIV-1 (22 and 59 events/ Figure Frequency of R5 and X4 HIV-1 infection in blood and thymic...
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Báo cáo y học: " Dendritic cell-mediated HIV-1 transmission to T cells of LAD-1 patients is impaired due to the defect in LFA-1" docx
... not result in activation of LFA-1, i.e the 'inside-out signaling' that is necessary for increased ICAM-1 binding is impaired [ 12, 20 -22 ] To further corroborate the importance of LFA-1 in HIV-1 transmission, ... Dustin ML: The immunological synapse Annu Rev Immunol 20 01, 19:375-396 Page of (page number not for citation purposes) Retrovirology 20 06, 3:75 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 ... Bates PA: Genetic analysis of integrin function in man: LAD-1 and other syndromes Matrix Biol 20 00, 19 :21 1 -22 2 Anderson DC, Springer TA: Leukocyte adhesion deficiency: an inherited defect in the Mac-1,...
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Dendritic cells respond differently to live and killed bacteria molecular mechanisms of pathogen recognition by dendritic cells and implications for vaccine development
... extraintestinal sites and to induce infections in organs They are involved in urinary tract infections (UTI), septicemia, diverse abdominal infections, meningitis (18 -20 ), bacteremia and sepsis (21 -23 ) ... in section 2. 7 Transfected HEK293T cells were fixed in 1% (w/v) paraformaldehyde and then permeabilized in 0 .2% (w/v) saponin for 10 The cells were blocked for 30 in 20 % goat serum (in PBS) and ... of some of these ligands has been identified using in vitro systems such as ligand binding assays or knockout mice Some of the known TLR ligands are listed in table 1.4 18 Table 1.4 TLRs ligands...
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Analysis and compensation of log_domain filter deviations due to transistor nonidealities
... outlined in Figure 2- 2 It begins with finding the passive prototype (Figure 2- 2(a)), followed by deriving the corre- spondinp linear SFG (Figure 2- 2(b)) and the log-domain SFG (Figure 2- 2(c)), and ... draw the corresponding linear SFG Routinely adding the LOG and EXP blocks according to the niles presented before, and after the following mappings are made, the log-domain SFG shown in Figure 2- 5(a) ... connotation of "lying somewhere between the familiar home temtories of the Iinear circuit and the formidable terrains of the nonlinear" [14] Chapter 1:Inrruduction 1 .2. 1 Translinear Principle Translinear...
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Children’s Health Deficits due to Diarrhoea: Effects of Water Supply and Sanitation Systems in Slums with Different Water Logging Conditions
... Corporation (Table and Figure 1) Thus, for the ‘code name , we used the initials of inundation duration, inundation type and the respective sub-district name of - 27 8 - Journal of Water and Environment ... survey) and, for mortality calculation, YLL = 62 years for male and 63 years for females in Bangladesh as of 20 05 (WHO 20 07) Furthermore, the default values for β, C and r have no influence for the ... defecating in open field rather than a latrine and without hand washing (with and without soap) before eating and after defecation However, control variables for mother’s illiteracy and household income...
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Energy savings due to daylight and artificial lighting integration in office buildings in hot climate
... and 18 kWh/m2/year for the north zone at 20 % window-to-wall ratio The difference becomes more pronounced as the window's area increases ISSN 20 76 -28 95 (Print), ISSN 20 76 -29 09 (Online) 20 11 International ... Bodart and A De Herde Global Energy Savings in Office Buildings by the Use of Daylighting Energy and Buildings 34 (20 02) 421 - 429 [6] E Ghisi and J A Tinker An Ideal window Area Concept for Energy ... thanks to King Fahd University of Petroleum and Minerals for its continuous support and for the use of its facilities ISSN 20 76 -28 95 (Print), ISSN 20 76 -29 09 (Online) 20 11 International Energy & Environment...
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The NCI Strategic Plan for Leading the Nation To Eliminate the Suffering and Death Due to Cancer pdf
... 1.3 — Increase our understanding of the behavioral, environmental, genetic, and epigenetic causes of cancer and their interactions A firm understanding of the underlying causes of cancer incidence, ... determine the role and function of individual genes, genomics is the study of the complete set of genes in the cell Likewise, proteomics is the study of all cellular proteins and their impact on the ... on understanding and modifying behaviors that increase risk, mitigating the influence of genetic and environ mental risk factors, and interrupting carcinogenesis through early medical intervention...
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Health Education to Villages: An integrated approach to reduce childhood mortality and morbidity due to diarrhoea and dehydration; Maharashtra, India 2005 – 2010 pptx
... continued monitoring of the training of health workers as well as the continual education of best practice information to the mother Correctly educating mothers, health workers, and children in the ... all of which is administered in the form of an IV, and the number of children who receive any form of ORT is negligible Using IV is expensive for both the health facility and the family, and in ... the need for informational programmes for mothers and supplemental training for health-care providers that emphasizes the importance of ORT, increased fluid intake, and continued feeding, and discourages...
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Báo cáo sinh học: "Functional characterization of human Cd33+ And Cd11b+ myeloid-derived suppressor cell subsets induced from peripheral blood mononuclear cells co-cultured with a diverse set of human tumor cell lines" docx
... 2. 27 Panc 2. 5 -1.79 6.08 MIA PaCa -2 -4.38 1.94 NCI-H1975 Small Cell 2/ 3) Mesothelioma (0/1) SW 1503 1.93 2. 02 Liver (2/ 5) * HA 22 T 44.01 4 .22 * HEP 3B 23 . 52 7.44 PLC 22 .05 5 .22 MAH 11.87 4. 72 ... influence of lung carcinoma cells Lechner et al Journal of Translational Medicine 20 11, 9:90 http://www.translational-medicine.com/content/9/1/90 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ... MD) for their generous contributions of cell lines used in these studies This work was supported by National Institutes of Health Training Grant Award 3T32GM067587-07S1, USC Institute for Innovation...
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Báo cáo sinh học: " Complement component C5a Promotes Expression of IL-22 and IL-17 from Human T cells and its Implication in Age-related Macular Degeneratio" potx
... expression in the serum of AMD patients [15,16], we found significantly increased levels of IL -22 and IL-17 in the sera of AMD patients, suggesting possible roles of IL -22 and IL-17 in the inflammation ... polarization [22 ,27 ,28 ] We found a significantly increased expression of both IL-1b and IL-6 in the supernatants of co-cultures containing both monocytes and T cells and an increased trend for TNF-a ... macular degeneration Proc Natl Acad Sci USA 20 05, 1 02: 722 7- 723 2 Jakobsdottir J, Conley YP, Weeks DE, Ferrell RE, Gorin MB: C2 and CFB genes in age-related maculopathy and joint action with CFH and...
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Báo cáo sinh học: " Constitutive expression of Atlantic salmon Mx1 protein in CHSE-214 cells confers resistance to Infectious Salmon Anaemia virus" ppt
... Binding of the influenza virus NS1 protein to double-stranded RNA inhibits the activation of the protein kinase that phosphorylates the Eif -2 translation initiation factor Virology 1995, 21 4 :22 2 -22 8 ... ampliconspecific melting peaks were observed in these samples The absence of the 22 0 bp band on agarose gel in these samples (Fig 3O, R, U) further confirmed the validity of melting curve analyses of these ... amplification The arrows in C and F indicate the expected band of 22 0 bp on agarose gel Amplification, melting curve, and agarose gel electrophoresis of RT-PCR targeting a 22 0-bp product on and HKS-36...
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báo cáo hóa học:" Constitutive expression of Atlantic salmon Mx1 protein in CHSE-214 cells confers resistance to Infectious Salmon Anaemia virus" docx
... Binding of the influenza virus NS1 protein to double-stranded RNA inhibits the activation of the protein kinase that phosphorylates the Eif -2 translation initiation factor Virology 1995, 21 4 :22 2 -22 8 ... ampliconspecific melting peaks were observed in these samples The absence of the 22 0 bp band on agarose gel in these samples (Fig 3O, R, U) further confirmed the validity of melting curve analyses of these ... amplification The arrows in C and F indicate the expected band of 22 0 bp on agarose gel Amplification, melting curve, and agarose gel electrophoresis of RT-PCR targeting a 22 0-bp product on and HKS-36...
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