action potential in brain cells
Báo cáo y học: " Helicobacter pylori induces mitochondrial DNA mutation and reactive oxygen species level in AGS cells"
... of pro-inflammatory cytokines from gastric epithelial cells resulting in the increased generation of ROS in gastric epithelial cells [24]. It has been shown that Hp infection can increase ... Medicine. Reagents F12 culture medium (Hangzhou Jino Biology Co., Ltd. China), fetal bovine serum (FBS), ampicillin, penicillin and streptomycin (Shanghai Bio-engineering Co., Ltd. China), ... smear and biochemical reactions, and the washed with 5 ml of brain heart infusion liquid medium. The eluate was incubated with brain heart infusion liquid medium containing 10% FBS and Hp selector....
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Báo cáo y học: "Proteomic analysis of mechanisms of hypoxia-induced apoptosis in trophoblastic cells"
... was examined using a fluorescent microscope. Fluorescence was mainly observed in cytosol areas in transfected cells. FITC-labeled Bag-1 Morpho/AS was transfected into almost all JAR cells by ... overexpressed in cytotrophoblasts, and a decrease in the expression of Bcl-2 and increase of apoptotic cells are seen in preeclampsia. Thus antiapoptotic proteins can play an important role in preventing ... shown in Fig. 3, 12hr exposure to hypoxia caused almost no increase in the phosphory-lated JNKs protein level in the cell line. Twenty-four-hour exposure to hypoxia resulted in a Int. J....
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Báo cáo y học: " Low concentration of ethanol induce apoptosis in HepG2 cells: role of various signal transduction pathways"
... Kinne RKH. Effects of doxorubicin, mitomycin C, and ethanol on Hep-G2 cells in vitro. J Cancer Res Clin Oncol 1999;125:1-8. 26. Higashi K, Hoshino M, Nomura T, et al. Interaction of protein ... microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976;72:248-54. 24. Pelech S, Sutter C, Zhang H. Kinetworks protein kinase multiblot analysis. Methods ... ethanol induces phosphorylation of protein kinases Table 1 shows the effect of 1 mM ethanol on protein kinases phosphorylation in HepG2 cells after 10 min exposure time compared to untreated cells. ...
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Báo cáo y học: " Mutation Analysis of hCDC4 in AML Cells Identifies a New Intronic Polymorphis"
... Elevated c-Jun expression in acute myeloid leukemias inhibits C/EBPalpha DNA binding via leucine zipper domain interaction. Oncogene. 2003;22(30):4760-4. 17. Staber PB, Linkesch W, Zauner D, Beham-Schmid ... acts as an adaptor protein providing substrate specificity for SCF (Skp–Cullin–F-box) ubiquitin ligases which are involved in tagging proteins for degradation in the proteasome. hCDC4 has been ... obtained at the time of their initial diagnosis after informed consent. For control, 51 PB samples were obtained from voluntary healthy individuals after informed consent. Mononuclear cells...
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Báo cáo y học: "PKC and PKA Phosphorylation Affect the Subcellular Localization of Claudin-1 in Melanoma Cells"
... involved in claudin-1 expression and localization. Indeed, a study shows that increasing β-catenin in- creases the expression of claudin-1 in colorectal cancer cells, and that claudin-1 expression ... claudin-1. Nuclear claudin-1 does not increase melanoma cell motility. We have previously shown that increasing the levels of CLDN-1 increases the invasion of melanoma cells (4). To determine ... claudin-1 in the cytoplasm, with some nuclear expression of claudin-1. B) Treatment of these cells with PKA inhibitor results in nuclear translocation of claudin-1 in as little as 15 minutes,...
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Báo cáo y học: "Transporter Molecules influence the Gene Expression in HeLa Cells"
... ace-tonitrile in water (B). The peptide was eluted with a successive linear gradient of 10% B to 80% B in 30 min at a flow rate of 23 ml/min. The fractions corre-sponding to the purified protein were ... cytoplasmic gene product is a serine/threonine-protein kinase N1 which is a member of the protein kinase C superfam-ily (http://amigo.geneontology.org/cgi-bin/amigo/gp-details.cgi?gp=UniProtKB:Q16512). ... Software and are described in the following part according the defini-tions of the Illumina’s (ILL) data as well as of the Il-lumina’s annotations (table 2). Int. J. Med. Sci. 2009, 6 ...
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Tài liệu Báo cáo khoa học: Interruption of triacylglycerol synthesis in the endoplasmic reticulum is the initiating event for saturated fatty acid-induced lipotoxicity in liver cells pdf
... carcinoma cells were grown in DMEM containing 10% heat-inactivated fetal bovineserum, 2 mm glutamine, 100 UặmL)1penicillin, and100 ngặmL)1streptomycin, at 37 C in air with 5% CO2. Cells ... Pharmigen, San Diego, CA, USA) binding andPI staining were performed according to [44]. Followingincubation with 1.0 lgặmL)1annexin V and 4.0 lgặmL)1PIfor 20 min, cells were analyzed by flow ... (SFAs) led to increased expression of proin-flammatory cytokines, inhibition of insulin signaling,induction of endoplasmic reticulum (ER) stress, andpromotion of cell death, mainly by apoptosis...
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Tài liệu Báo cáo khoa học: Loose interaction between glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate kinase revealed by fluorescence resonance energy transfer–fluorescence lifetime imaging microscopy in living cells doc
... the length of linkers. In the linker con-taining seven amino acids, a Pro-Pro sequence that isnot contained in other linkers is incorporated. This lessflexible structure may inhibit fluorophores ... linked tocerulean or citrine were introduced into CHO-K1 cells. As shown in Figs 1A and S1A, transiently expressedGAPDH–citrine (chimeric protein with N-terminalGAPDH and C-terminal citrine) ... relative ori-entation of two fluorophores, being independent of thestrength of protein–protein interactions. In the case ofvery weak interactions as described in this article,rapid association and...
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Tài liệu Báo cáo khoa học: Shaped by the environment – adaptation in plants Meeting report based on the presentations at the FEBS Workshop ‘Adaptation Potential in Plants’ 2009 (Vienna, Austria) pdf
... thought by providingjust such an interdisciplinary forum, in which researchresults, including novel concepts such as environmen-tally induced increases in mutation rates in bacteriaand a ... gene regulonsand regulatory pathways involved in freezing tolerance in Arabidopsis. Freezing tolerance increases in manyplants in response to low, non-freezing temperature, aprocess known as ... exposuredepending on the time of day of the exposure [51].Cold induction of CBF2 involves multiple cis-actingregulatory elements, one of which binds members ofthe calmodulin-binding transcription...
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Tài liệu Báo cáo khoa học: Collagen I regulates matrix metalloproteinase-2 activation in osteosarcoma cells independent of S100A4 pdf
... determine the viability of the cells, trypsinized cells weresuspended in serum-containing medium. In order to removeserum, cells were washed three times with serum-free med-ium prior to seeding ... osteosarcoma cells in our study wherethe cells appeared more rounded in shape. In humanskin fibroblasts there is an increase in proMMP-2 acti-vation that is independent of the lattice contraction[23]. ... MMPs are secreted as inactive proenzymes andlatency is maintained by an interaction between acystein residue in the prodomain and Zn2+ in theactive site of the catalytic domain. Two major types...
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Tài liệu Báo cáo khoa học: Expression of poly(A)-binding protein is upregulated during recovery from heat shock in HeLa cells doc
... specific peptide-binding domain found in poly(A)-binding protein and aHECT ubiquitin ligase. EMBO J 23, 272–281.9 Craig A (1998) Interaction of polyadenylate-bindingprotein with the elF4G ... fractions. In contrast, almost 90–100% of the b-actin mRNA waspresent in the polysomal fractions. There was a signifi-cant reduction in the translation of the b-actin mRNA in heat-shocked cells, ... somecircumstances [32,33]. A small fraction of eIF4G hasbeen previously shown to be present in the nuclearfraction, and to bind the cap-binding complex involved in splicing [34]. It is believed that...
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Tài liệu Báo cáo khoa học: Lipopolysaccharide-evoked activation of p38 and JNK leads to an increase in ICAM-1 expression in Schwann cells of sciatic nerves ppt
... to an increase in ICAM-1 expression in Schwann cells of sciatic nervesAiguo Shen1,*, Junling Yang2,*, Yangyang Gu3, Dan Zhou4, Linlin Sun2, Yongwei Qin2,Jianping Chen2, Ping Wang2, ... Schwann cells) and CD31 (a marker of endothelial cells) . In pre-vious studies, ICAM-1–integrin interactions mediatedadhesion of leukocytes to the vascular endothelium,revealing a key role in migration ... of cytokineinduced expression of intercellular adhesion molecule 1(ICAM-1) in an intestinal epithelial cell line, a processblocked by proteasome inhibitors. Gut 42, 779–787.23 Lakshminarayanan...
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Tài liệu Báo cáo khoa học: Bone morphogenetic protein signaling in stem cells ) one signal, many consequences pptx
... protein-inducedT. U. Wagner BMP signaling in stem cells FEBS Journal 274 (2007) 29682976 ê 2007 The Author Journal compilation ª 2007 FEBS 2975 MINIREVIEWBone morphogenetic protein signaling in ... neuroepithelial cells into astrocytes, by binding and hyper activatingthe promoter of glial fibrillary acidic protein (GFAP). In parallel, BMP signals lead to enhanced expressionof ID proteins, which in ... triggering.There is another example of stemness decisions byNodal versus BMP signaling emerging from humanembryonic stem cell research. Testing human ES -cells BMP signaling in stem cells T....
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Tài liệu Báo cáo khóa học: Suppression of b1,3galactosyltransferase b3Gal-T5 in cancer cells reduces sialyl-Lewis a and enhances poly N-acetyllactosamines and sialyl-Lewis x on O-glycans Lydia Mare and Marco Trinchera pdf
... in Dulbeccos modied Eagles mediumcontaining 10% foetal bovine serum, 100 UặmL)1penicillin,1.0 mgặmL)1streptomycin and 2 mML-Glu. For treatingBxPC3 cells and clones with drugs affecting ... (under-lined), and a 27-base sequence corresponding to nucleotide334–360 in the coding region of the gene. PCR reactionswere incubated as follows: 94 °C for 3.5 min followed by 25cycles of 1.5 min ... by staining with ethidium bromide. (B)b1,3Gal-T activity in BxPC3 cells (j)orinT5ASclone(h)wasdetermined with GlcNAc as acceptor using different amounts of cellhomogenates for a fixed incubation...
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Tài liệu Báo cáo khoa học: Extended half-life upon binding of destabilized intrabodies allows specific detection of antigen in mammalian cells pdf
... FranceIntracellular antibodies or intrabodies are antibodyfragments used inside cells for interaction with targets. In case intrabodies interfere with antigen function,they can mediate cell killing ... fused to d1-EGFP are maintained in the cells by binding to antigen.Generation of soluble binding-defectiveand cysteine-deleted mutantsTo demonstrate that the observed intracellular colocal-ization ... cell lines(Flip -In TMcell lines; Invitrogen, Cergy Pontoise, France)were maintained in Dulbecco’s modified Eagle’s tissue cul-ture medium (DMEM; Invitrogen) supplemented withl-glutamine (2...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến hiệu suất h fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25