a novel antimicrobial abietane type diterpene from salvia albocaerulea
Báo cáo khoa học: A novel antifungal hevein-type peptide from Triticum kiharae seeds with a unique 10-cysteine motif doc
... CGCTGGTTCTTGCCAGTCTCAGTGCCGTGGTTGCTAGGGAT TTTAGCACCACGAGCCTGGTCACCGCAACGCTGAGC CGCAGAAGCCGTACTTACCACAGCACAGGCAGTTCG GACTGGCAAGAACCAGCGCCACAGTAAGCGTCACCA GCTAGGATCCCTAGCAACCACGGCAC Table Antifungal activity ... Escherichia coli and assays of recombinant WAMP 1a activity against diverse plant pathogens, such as chitin-containing and chitin-free fungi, and Grampositive and Gram-negative bacteria The amino acid ... Odintsova et al chitinases and 1,3-b-glucanases, proteinases and some other enzymes, proteinase and a- amylase inhibitors, thaumatin-like proteins and antimicrobial peptides (AMPs) AMPs form a highly...
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Báo cáo khoa học: Purification and characterization of three isoforms of chrysophsin, a novel antimicrobial peptide in the gills of the red sea bream, Chrysophrys major doc
... It has been proposed that amphipathic a- helical peptides show antimicrobial activity by interacting electrostatically with the anionic bacterial membrane, adopting an amphipathic a- helical conformation ... (Tokyo, Japan), an Inertsil C8-3 column from GL Science (Tokyo, Japan), and a Capcellpak C18 column from Shiseido (Tokyo, Japan) SP-Sephadex C-25 was purchased from Pharmacia Biotech (Uppsala, Sweden), ... KCl CA, USA) by analyzing the data calibrated with 10 pmol phenylthiohydantoin amino-acid standards Tricine/SDS/PAGE The molecular mass of the sample was estimated by Tricine/ SDS/PAGE using a 16.5%...
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Báo cáo Y học: Bass hepcidin is a novel antimicrobial peptide induced by bacterial challenge pptx
... Kel, A. E., Kel, O.V., Ignatieva, E.V., Ananko, E .A. , Podkolodnaya, O .A. , Kolpakov, F .A. , Podkolodny, N.L & Kolchanov, N .A (1998) Databases on transcriptional regulation: TRANSFAC, TRRD, and COMPEL ... Briefly, DNA was double-digested with DraI and HpaI, incubated with T4 ligase (Promega) to create intramolecular ligations, and amplified with a primer pair 158F and 86R Amplification and sequence ... domains are proposed for bass preprohepcidin: (a) a hydrophobic signal peptide (24 amino acids); (b) a prodomain (40 amino acids); and (c) a mature peptide (21 amino acids) (Fig 3) A canonical...
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Phytochemistry volume 65 issue 20 2004 doi 10 1016 j phytochem 2004 08 024 marie c yimdjo; anatole g azebaze; augustin e nkengfack; a m antimicrobial and cytotoxic agents from calophyllum i
... Gunatilaka, A. A.L., Desilva, A. M.Y.J., Sotheeswaran, S., Balasubramaniam, S., Wazeer, M.I.M., 1984 Terpenoids and biflavonoids constituents of Calophyllum calaba and Garcina spicata from Sri Lanka ... (ATCC19264), Gram-(À), Escherichia coli (ATCC8739) bacteria, and yeast, Candida tropicalis (ATCC 66029) organisms, in agar well diffusion assays The results are summarized in Table At the dose of ... Bioassays 3.4.1 Antimicrobial assay The extracts and purified active principles from C inophyllum were tested against the microorganisms, S aureus (ATCC6538), V angillarium (ATCC19264), E coli (ATCC8739),...
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Tài liệu Báo cáo khoa học: A novel type of highly negatively charged lipooligosaccharide from Pseudomonas stutzeri OX1 possessing two 4,6-O-(1-carboxy)-ethylidene residues in the outer core region ppt
... oligosaccharides from Pseudomonas strains have already been isolated and characterized [12,13], mainly from Pseudomonas aeruginosa strains [21,37–43] The carbohydrate backbone of the so-called ... carbohydrate backbone from alkaline degradation of the rough form LPS from P stutzeri OX1 Isolation, NMR and MS analyses of oligosaccharide from acetic acid hydrolysis Further information on alkaline labile ... 4,6-disubstituted-HexN and, in small amounts, terminal-Rha and 6-substituted-Glc In addition, the disaccharide 7-O-carbamoyl-Hep-(1fi3)Hep was found Fatty acid analysis revealed the presence of typical fatty acids...
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Báo cáo khoa học: A novel mechanism of V-type zinc inhibition of glutamate dehydrogenase results from disruption of subunit interactions necessary for efficient catalysis doc
... with alternative amino acid substrates GDH from mammalian sources is highly regulated by a diverse array of small molecules, with ADP, GTP, leucine and the combination of malate and palmitoyl CoA ... phosphate buffer, pH 7.0, 0.5 mM NADP+, 20 mM glutamate amplitude and rate constant for the pre-steady state phase to be calculated The parameters for both the steady state phase and the pre-steady ... negated by the small increase in stability caused by glutamate Norvaline, to a much greater effect, stabilizes the protein and again zinc has a minimal effect Although zinc does not cause large...
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Báo cáo Y học: Hemocyanin from the keyhole limpet Megathura crenulata (KLH) carries a novel type of N-glycans with Gal(b1–6)Man-motifs doc
... Hex4HexNAcPA Hex5HexNAcPA Hex6HexNAcPA Hex5GlcNAc2PA Hex7HexNAcPA Hex6GlcNAc2PA Hex5HexNAc3PA Hex2HexNAc2PA Hex3HexNAc2PA Hex6HexNAc2PA Hex2HexNAc2dHexPA Hex3HexNAc2PA Hex3HexNAc2dHexPA Hex4HexNAc2PA ... Peptide-N4-(N-acetyl-b-glucosaminyl)asparagine amidase A from almond (PNGase A) was from Seikagaku (Tokyo, Japan) and b-galactosidase from jack beans was obtained from Glyco (Upper Herford, UK) Tryptic ... separately pyridylaminated and designated endoH-PA, PNGaseF-PA, PNGaseA-PA and Hyd(HFA)-PA, respectively Analytical anion-exchange HPLC of the pyridylaminated oligosaccharide pools demonstrated...
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Báo cáo y học: " Identification of a novel motif responsible for the distinctive transforming activity of human T-cell leukemia virus (HTLV) type 1 Tax1 protein from HTLV-2 Tax2" pps
... http://www.retrovirology.com/content/6/1/83 Iwanaga Y, Tsukahara T, Ohashi T, Tanaka Y, Arai M, Nakamura M, Ohtani K, Koya Y, Kannagi M, Yamamoto N, Fujii M: Human T-cell leukemia virus type tax protein abrogates interleukin-2 ... HTLV-4 are associated with any particular disease such as leukemia The amino acid sequence and the functional analysis of HTLV-3 Tax3 showed that Tax3 has a functional PBM, and is capable of interacting ... 80 80 Transformation (Number) 60 40 20 40 20 Tax 224 Tax 232 Tax Tax 300 Tax2B Tax2B Tax 207 Tax300 D) Tax 154 Tax232 Tax1 Tax224 Tax 207 Tax207 Tax 184 Tax184 Tax154 Tax1 Tax1 Tax 154 Tax207 B)...
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Tài liệu Báo cáo khoa học: A novel electron transport system for thermostable CYP175A1 from Thermus thermophilus HB27 doc
... thermophilus E coli A pernix 277 277 283 TSIPGVYACGDIVTYPGKLPLIVLGFGEAAIAANHAAAYAN-PALKVNPGHSSEKAAPGT TSIPGVFAAGDVMDHI YRQAITSAGTGCMAALDAERYLD GLADAK TSIPGIFAAGDCTSMWPGFRQVVTAAAMGAVAAYSAYTYLQEKGLYKPKPLTGLK ... (Osaka, Japan) Emulgen 911 was a gift from Kao Chemical (Tokyo, Japan) NADPH, NADH and NADP+ were purchased from Oriental Yeast (Tokyo, Japan) a- Cyano-4-hydroxycinnamic acid was obtained from ... FNR from Rhodobacter capsulatus, AAF35905; ADR from Homo sapiens, AAB59498; adrenodoxin reductase from Saccharomyces cerevisiae, AAB64812; FprA from Mycobacterium tuberculosis, O05783; BphA4 from...
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Tài liệu Báo cáo khoa học: Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 docx
... A novel high-activity D-hydantoinase from Jannaschia sp CCS1 obtain optically pure amino acids, namely chemical and enzymatic syntheses Chemical synthesis gives racemic mixtures of amino acids ... HYDJs and DCase Although almost all hydantoinases that are currently applied in industry were obtained from microbial sources, the exact metabolic function and natural substrates of hydantoinases ... subunit molecular mass was also estimated by SDS–PAGE Characterization and comparative analyses of HYDJs and HYDBp The optimal temperature for activity of HYDJs with d-pHPH as substrate was determined...
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Tài liệu Báo cáo khoa học: N-Methyl-L-amino acid dehydrogenase from Pseudomonas putida A novel member of an unusual NAD(P)-dependent oxidoreductase superfamily ppt
... were of analytical grade from Nacalai Tesque (Kyoto, Japan) and Wako Pure Chemical Industries (Osaka, Japan) Culture and screening of bacteria Bacterial strains were cultivated for 1520 h at 30 ... N-Methyl-L-amino acid dehydrogenase from P putida H Mihara et al ammonia are formed from methylamine and glutamate by N-methylglutamate synthase (EC 2.1.1.21) [16] Another A aminovorans strain, formerly ... 50-lL reaction mixture containing 1ã LA Taq buffer (Takara Shuzo), 2.5 mm MgCl2, 0.4 mm dNTP, 0.2 mm each primer (5Â-GGAAT TCCATATGTCCGCACCTTCCACCAGCACCG-3Â and 5Â-GGGAAGCTTTCAGCCAAGCAGCTCTTTCAGG-3Â),...
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Tài liệu Báo cáo khoa học: A novel nuclear DNA helicase with high specific activity from Pisum sativum catalytically translocates in the 3¢fi5¢ direction docx
... cross-react with antibodies against plant helicases including PDH45 and PDH65 and also against human DNA helicases I, II, III and IV (data not shown) ssDNA-dependent ATPase activity was present at a ... and ssDNA cellulose were from Whatman and Pharmacia; T4 polynucleotide kinase and DNA polymerase I were from New England Biolabs; trypsin was from Serva (Heidelberg, Germany); the DNA-intercalating ... 2A, lane 4) and ssDNA-dependent ATPase activity (data not shown) sedimented together between alcohol dehydrogenase and BSA (fraction 11) and gave a molecular mass of 120 kDa with a sedimentation...
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Tài liệu Báo cáo Y học: A novel meta-cleavage dioxygenase that cleaves a carboxyl-groupsubstituted 2-aminophenol Purification and characterization of 4-amino-3-hydroxybenzoate 2,3-dioxygenase from Bordetella sp. strain 10d doc
... Morphological and phenotypic characterization Physiological and biochemical parameters, such as Gram reaction, flagella type, catalase activity, oxidase activity and OF test, were determined using classical ... sucrose Alkali was produced from L-asparagine, citrate, galactarate and tartrate The nucleotide sequence (1457 bp) of the 16S rRNA gene of strain 10d was 96.7% identical with that of Bordetella avium ... REFERENCES Fujisawa, H & Hayaishi, O (1968) Protocatechuate 3,4-dioxygenase I Crystallization and characterization J Biol Chem 243, 2673–2681 Murakami, S., Nakanishi, Y., Kodama, N., Takenaka, S., Shinke,...
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Tài liệu Báo cáo Y học: A novel, inducible, citral lyase purified from spores of Penicillium digitatum docx
... HA and DEAE were both operated with a Gradifac system (Pharmacia Biotech, Roosendaal, the Netherlands) Fig Reaction catalysed by citral lyase, combining hydratase and aldolase activity (A) , from ... Harayama, S (1998) Biochemical and genetic characterization of trans-2¢-carboxybenzalpyruvate hydratasealdolase from a phenanthrene-degrading Nocardioides strain J Bacteriol 180, 945–949 33 Laurie, ... of citral at high pH [18] For the enzymatic equivalent of this reaction the actions of a hydratase and an aldolase are needed Citral lyase of P digitatum combines hydratase and aldolase activity...
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Tài liệu Báo cáo khoa học: Characterization and functional expression of cDNAs encoding thyrotropin-releasing hormone receptor from Xenopus laevis Identification of a novel subtype of thyrotropin-releasing hormone receptor ppt
... set, A5 1 (5¢GATGTCACGCAGAGTGAGCAGGTAG-3¢)/TRHR-7 (5¢-GAGACCATACAGAAC-C-3¢); second set, A5 2 (5¢AGAGTGAGCAGGTAGCGAGAGGAG-3¢)/TRHR-8 (5¢-GGGGGTGTAGAGGTTTCTGGAGAC-3¢); third set, A5 3 (5¢-CGAGAGGAGCATTAGA-TAGATG ... amplified as described above using partially overlapping cDNA fragments and the pair of primers TRHR1-2 sense (5¢-ATAATGGATAA CGTAACTTTTGCTG-3¢)/TRHR1-4 antisense (5¢-TC TGTTAAATGTACCTAAGTAGGCA-3¢) and ... 32281–32287 31 Itadani, H., Nakamura, T., Itoh, J., Iwaasa, H., Kanatani, A. , Borkowski, J., Ihara, M & Ohta, M (1998) Cloning and characterization of a new subtype of thyrotropin-releasing hormone...
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Báo cáo khoa học: A novel metallocarboxypeptidase-like enzyme from the marine annelid Sabellastarte magnifica – a step into the invertebrate world of proteases pdf
... gamus, Molgula occidentalis and Pyura vittata); 11 species of Cnidaria (Bartholomea annulata, Budonosoma granulifera, Cassiopea xamachana, Condylactys gigantea, Gorgonia ventalina, Lebrunia danae, ... Mollusca, Echinodermata, Arthropoda and Chordata, amongst others, collected on the coasts of Havana, Cuba The 4876 Fig S magnifica Phylum Annelida, Class Polychaeta, Subclass Palpata, Order Canalipalpata, ... danae, Palythoa caribaeorum, Physalia physalis, Plexaura homomalla, Stichodactyla helianthus and Zoanthus pulchellus); two species of Annelida (Sabellastarte magnifica and Hermodice carunculata);...
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Báo cáo khoa học: Purification and structural analysis of the novel glycoprotein allergen Cyn d 24, a pathogenesis-related protein PR-1, from Bermuda grass pollen pot
... E GAG A GCC T ACC L TTG A GCC Y TAC E GAG D GAC H CAC D GAC Q CAG A GCA S AGC V GTC S TCC A GCT N AAC K AAG A GCG N AAC E GAG Y TAC D GAC K AAG L CTA K AAG Q CAG L CTC G GGC G GGG E GAG E GAG ... YVSP AA AVG.GAVS.S.K.Q 54 Triticum MQTPKLAILLALAMSAAMANLSQAQNSP Y.SP AA AVG.GAV S.K.Q 54 Zea -MAPRLACLLALAMAAIVVAPCTAQNSP YVDP AA DVG.G.VS.D V 53 Nicotiana MGFVLFSQLPSFLLVSTLL.FLVISHSCRAQNSQ ... CTG K AAG T ACC K AAG P CCC V GTG S AGC T ACC T ACA G GGT L CTC R CGC S TCG M ATG K AAG A GCC K AAG Y TAC I ATT F TTC A GCC T ACA S AGC N AAC V GTC K AAG F TTC S AGC V GTC D GAC L CTC G GGC A GCC...
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Báo cáo khoa học: Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL family docx
... composed of parallel b strands associated to an antiparallel strand (b2) and is surrounded by helices (a1 , a2 , a3 , a7 and a8 ) The second domain consists of helices a4 , a5 and a6 all clustered ... triad made of a catalytic nucleophile serine, associated to a proton carrier histidine and a charge relaying aspartic (or glutamic) acid To further investigate the biochemical characterization ... vinyl butyrate (20.7 mM) as a substrate The pH value was adjusted and the enzyme (7.4 lg) was added in the vessel Activity assays at acidic pH were corrected using the calculated pKa value of 4.87...
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Báo cáo khoa học: Phaiodotoxin, a novel structural class of insect-toxin isolated from the venom of the Mexican scorpion Anuroctonus phaiodactylus pdf
... degradation carried out with an automatic apparatus Beckman LF 3000 Protein Sequencer (Palo Alto, CA, USA), using the peptide adsorbed on CD Inmmobilon membranes (Beckman part number 290110) A sample ... Ambion (Austin, TX, USA) The cDNA mix was synthesized from poly (A) + mRNA using M-MLV reverse transcriptase The cDNA was joined with the adaptor provided by the kit (5¢-gcugauggcgaugaaugaacacugcguuugCUGG ... phaiodotoxin Data are presented as a Boltzmann sigmoidal fit Each point represents the mean ± SEM of data from five experiments (C) Superimposed graphics of the activation and steady-state inactivation...
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Báo cáo khoa học: The male seahorse synthesizes and secretes a novel C-type lectin into the brood pouch during early pregnancy pdf
... was pulled away from the muscle wall, and RNA was extracted using TRIzol (Invitrogen, Carlsbad, CA) The cDNA library was constructed using MMLVreverse transcriptase (Stratagene, La Jolla, CA), ... primers: forward, 5¢-CGCGGATCCTGGTCTTTCCAAAATATTC AGGCCA-3¢ and reverse, 5¢-GTCCTCGAGGTACATCA CATCTCTGAT-3¢ After digestion, the PCR-amplified fragment was cloned into a modified BamHI ⁄ XhoI digested ... pouches of five male seahorses (Hippocampus comes: purchased from Pacific Marine Aquaria, Singapore, after import from Indonesia) at various stages of pregnancy After removal of all embryos, the...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct các đặc tính của động cơ điện không đồng bộ đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008