... presence of 50 m m dithiothreitol.Ten microliters of each sample lysate was transferred intoeach of two eppendorf tubes and treated for 15 min witheither 10 lL of the 1· DNPH solution or 10 lL of ... degradationmachines of the cell. Trends Biochem Sci 19, 377–382.23 Bence NF, Sampat RM & Kopito RR (2001) Impair-ment of the ubiquitin-proteasome system by proteinaggregation. Science 292, 155 2– 155 5.24 ... et al. (2004) Inhibition of mTOR induces autophagyand reduces toxicity of polyglutamine expansions in flyand mouse models of Huntington disease. Nat Genet 36, 58 5 59 5.20 DiFiglia M, Sapp E,...
... controlunheated Hsp27-3D and Hsp27-3D heated to 70 °C, both at a con-centration of 0 .5 mgặmL)1. Curves 37 correspond to Hsp27-3D(1 mgặmL)1) heated in the presence of 0. 25, 0 .5, 1.0, 2.0 ... F-actin (0 .5 mgặmL)1) was heated ata constant rate of 1 Cặmin)1in the presence of Hsp27-3D(0.1 25 mgặmL)1), and Rhwas plotted as a function of temperature.(B) After being heated to 85 °C, ... of a small heat shock protein. Nature 394, 59 5 59 9.8 Jakob U, Gaestel M, Engel K & Buchner J (1993) Small heat shock proteins are molecular chaperones. J BiolChem 268, 151 7– 152 0.9 Ganea...
... types of latent heat. The first is the latent heatof fusion. Thisis the amount ofheat added or removed to change phase between solid and liquid. The secondtype of latent heat is the latent heat ... ENERGY, WORK, AND HEAT Another type ofheat is called latent heat. Latent heat is the amount ofheat added to or removedfrom a substance to produce a change in phase. When latent heat is added, ... different magnitudes by the addition of heat. When a givenamount ofheat is added to different substances, their temperatures increase by different amounts.The ratio of the heat (Q) added to or...