... One approach to increase the duration of PNA binding to DNA isto conjugate it toa DNAalkylating reagent so that the PNA becomes covalently bound to its target sequence To this a DNA alkylating ... that mitoDC81 alkylates mtDNA in mitochondria or cells The reasons for the lack of alkylation of mtDNA within mitochondria by mitoDC-81 are unclear The local concentrations of mitoDC-81 and DNA, ... DNA ladder by mitoDC-81 (Fig 3B, lane 1) Analysis ofthe gel prior to electrotransfer showed that the extent of alkylation was proportional tothe amount of DNA present and was independent of the...
... significantly tothe disappearance of nitrotyrosinated tubulin, the tyrosination/detyrosination at the C-terminus of a- tubulin isthe main operating mechanism Furthermore, when cells maximally nitrotyrosinated ... indicates clearly that the two compounds are incorporated into tubulin at the same site Another biochemical characteristic of tubulin is its ability to act as substrate ofthe detyrosinating ... incubation was the same in both cases (data not shown), indicating that replacement of tyrosine by 3-nitrotyrosine at the C-terminus of a- tubulin is not relevant tothe association of carboxypeptidase...
... human cells J Cell Sci 108, 635– 644 10 Jagatheesan G, Thanumalayan S, Muralikrishna B, Rangaraj N, Karande AA & Parnaik VK (1999) Colocalization of intranuclear lamin foci with RNA splicing factors ... and characterization ofa novel zinc finger protein that associates with nuclear matrix DNA Cell Biol 17, 849 –858 Lee JY, Nakane Y, Koshikawa N, Nakayama K, Hayashi M & Takenaga K (2000) Characterization ... with Coomassie Brilliant Blue A4 A6 are the same as A1 A3 except that the samples were blotted onto a nitrocellulose sheet, incubated with anti-His sera and then alkaline phosphatase-conjugated secondary...
... e e e e e e e e e e 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 aaaaaaaaaaaaaaaaaaaaaaaaaaa b b b b b b b b b b b b b b b b b b b ... area ofa circle, use the formula A = r2 r ■ To find the area ofa parallelogram, use the formula A = bh h b ■ To find the area ofa rectangle, use the formula A = lw w l ■ To find the area ofa ... find the volume ofa cylinder, use the formula V = r2h h r S URFACE A REA The surface area of an object measures the combined area of each of its faces The total surface area ofa rectangular...
... in addition to their native language The term “second language” is used to describe the acquisition of any language after the acquisition ofthe mother tongue There is also research into the ... likely to lead to dissatisfaction and alienation and not facilitate personal, social and academic growth; the best result are likely to occur when there isa combination of warmth and accomplishments ... instrumental motivation, the purpose of language acquisition is more utilitarian It is often characteristic of language learning acquisition, where little or no social integration ofthe learner into a...
... child mortality, improving maternal health and combating HIV, malaria and other diseases The attainment of these goals has been hampered by the lack of skilled and well-informed health care workers ... handbook and this paper, drafting the handbook and this paper, and have approved the final version ofthe paper Acknowledgements We thank David Baume and Ian Willis for their helpful inputs to ... standards These are summarized below Quality assurance of recruitment and admissions Aim To ensure that courses are accessible tothe entire community and that the admissions procedures are fair,...
... clinical data into a diagnosis Another example was that incomplete documentation, which could confuse pharmacists and caretakers, was relatively common The analysis also revealed that some potentially ... we used a simple graphical pathway analysis In quality improvement methodologies, this is conceptually similar toa "rootcause" analysis [ 24] We began with the ideal case-management pathway IMCI ... Definitions ofthe indicators of pneumonia case-management quality Definitions ofthe indicators of pneumonia case-management quality To examine specific causes of common errors in the casemanagement...
... and the average duration of FM was years In study 1077, the mean age of patients was 50.1 years and the average dura- Table 1: Baseline patient characteristics and sleep scores Characteristics ... possible pain") Further, study patients had to have a score of at least 40 mm on the 100 mm visual analogue scale (VAS) ofthe Short-Form McGill Pain Questionnaire [ 14] at the screening and baseline ... clinical trials During the baseline phase, study patients had to have an average daily diary pain score of at least (within the last days) on a numeric rating scale ranging from ("no pain") to 10...
... into Spanish Arthritis Rheum 1995, 38: 142 9- 144 6 26 Garc a- Campayo J, Pascual A, Alda M, Marzo J, Magallón R, Fortes S: The Spanish version ofthe fibrofatigue scales: validation ofa questionnaire ... Questionnaires analysis FIQ An exploratory factor analysis with a Kaiser-MeyerOlkin (KMO) index of 0.83, yielding two factors that explained 57% ofthe variance The first explains 44 % ofthe variance ... conceived the study MAV designed the study and perform the statistical analysis These authors revised the data obtained and draft the manuscript MAV coordinated the analysis, results and discussion All...
... was calculated as the ratio ofthe actual arm trajectory length tothe shortest path tothe target A ratio of indicates no arm deviation from the shortest path, while a ratio of indicates that ... thigh and shank, was analysed as a single segment since the angular displacement at the knee joint was minimal for this task The vector of temporal variation ofthe segmental angles i around their ... ultimate goal isto collect as many characters as possible and get the final reward, an animated Mermaid, who is sitting on the coral reef and waving her tail All these actions are added to slightly...
... S, Vaughn DW, Kalayanarooj S, Nisalak A, Norman JE, Ennis FA, Rothman AR: Analysis of plasma viral RNA levels during acute dengue virus infection using quantitative competitor reverse transcription-polymerase ... 13:1185-1189 Kumarasamy V, Chua SK, Hassan Z, Huahab AHA, Chem JK, Mohamad M, Chua KB: Evaluating the sensitivity ofa commercial dengue NS1-antigen capture ELISA for early diagnosis for acute dengue ... determined at 45 0 nm using an automatic ELISA plate reader (Multiskan EX, Labsystems) Negative, positive and cut-off control reagents provided with the kit were run each time for validation ofthe assay...