... tion, 10.0mL of CaCl2 solution, 10.0mL of MgSO4·7H2O solution, 10.0mL of NaNO3 solution, 10.0mL of K2HPO4 solution, 10.0mL of KH2PO4 solution, and 10.0mL of NaCl solution to distilled/deionized ... Preparation of FeCl3 Solution: Add FeCl3 to distilled/deionized water and bring volume to 100.0mL Mix thoroughly Preparation of Bristol's Solution: Add 10.0mL of NaNO3 solu- tion, 10.0mL of CaCl2 ... at 15 psi pressure–121°C Cool to 50°–55°C Preparation of Medium: Aseptically combine 500.0mL of solution A, 1.0mL of solution B, and 500.0mL of solution C Mix thoroughly Pour into sterile Petri...