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Handbook of Microbiological Media, Fourth Edition part 14 pdf

Handbook of Microbiological Media, Fourth Edition part 14 pdf

Điện - Điện tử

... dilution assay of pen-icillins and other antibiotics. Used in the turbidimetric assay of penicillinand tetracycline with Staphylococcus aureus. For the cultivation and main-tenance of Bacillus ... 11.0gPancreatic digest of casein 10.0gYeast extract 6.5gPancreatic digest of gelatin 5.0gK2HPO4 3.68gNaCl 3.5gBeef extract 1.5gKH2PO4 1.32gpH 6.6 ± 0.2 at 25°CPreparation of Medium: Add ... assay of carbenicillin, colistimethate, and polymyxin B.Antibiotic Medium 11(Neomycin Assay Agar)Composition per liter:Agar 15.0gPancreatic digest of gelatin 6.0gPancreatic digest of casein...
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Handbook of Microbiological Media, Fourth Edition part 30 pdf

Handbook of Microbiological Media, Fourth Edition part 30 pdf

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... Aseptically add 0.05g of chloramphenicol.Mix thoroughly. Pour into sterile Petri dishes.Use: For the isolation and presumptive identification of Cryptococcusneoformans. Cryptococcus neoformans appears ... waterand bring volume to 200.0mL. Mix thoroughly. Preparation of Medium: To 400.0mL of solution A, add 400.0mL of solution B and 200.0mL of solution C. Adjust pH to 5.0. Add agar.Mix thoroughly. ... 20.0mL of sterile car-bohydrate solution, 10.0mL of sterile L-cysteine·HCl·H2O solution,and 10.0mL of sterile Na2S·9H2O solution or, using a syringe, injectthe appropriate amount of sterile...
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Handbook of Microbiological Media, Fourth Edition part 31 pdf

Handbook of Microbiological Media, Fourth Edition part 31 pdf

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... inhalation.Preparation of Medium: To 950.0mL of cooled sterile basal medi-um, aseptically add 50.0mL of lysed (fresh, frozen, and thawed) horseblood, 4.0mL of sterile FBP solution, 8.0mL of sterile cefoperazone ... inhalation.Preparation of Medium: To 950.0mL of cooled sterile basal medi-um, aseptically add 50.0mL of lysed (fresh, frozen, and thawed) horseblood, 4.0mL of sterile FBP solution, 4.0mL of sterile cefoperazone ... 4.0mL of sterile cefoperazone solution, 4.0mL of sterile trimethoprim lactate solution, 4.0mL of sterile vancomycinsolution, and either 4.0mL of sterile cycloheximide solution or 4.0mL of sterile...
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Handbook of Microbiological Media, Fourth Edition part 32 pdf

Handbook of Microbiological Media, Fourth Edition part 32 pdf

Điện - Điện tử

... to 25°C. Add 0.5mL of sterilecarbohydrate solution to each tube.Use: For the determination of carbohydrate fermentation reactions of microorganisms, particularly members of the Enterobacteriaceae. ... 2.0mgPolymyxin B 2500UPreparation of Antibiotic Supplement: Add components to10.0mL of distilled/deionized water. Filter sterilize.Preparation of Medium: Prepare 890.0mL of Brucella agar base.Sterilize ... pres-sure–121°C. Cool to 45°–50°C.Preparation of Medium: To 890.0mL of cooled, sterile Columbiaagar base, aseptically add 100.0mL of sheep blood or 50.0–70.0mL of laked horse blood. Laked blood is prepared...
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Handbook of Microbiological Media, Fourth Edition part 33 pdf

Handbook of Microbiological Media, Fourth Edition part 33 pdf

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... a holding medium of clinical speci-mens during collection or shipment.Caryophanon latum MediumComposition per liter:Papaic digest of soybean meal 2.0gPancreatic digest of casein 2.0gYeast ... Aseptically add 1.0mL of sterile trace salts solution and 5.0mL of sterile lincomycin solution.Mix thoroughly. Aseptically distribute into sterile tubes or flasks.Use: For the cultivation of heat-labile, ... the cultivation of Acetobacter pasteurianus.Carrot Decoction AgarComposition per liter:Carrots 100.0gAgar 15.0gPreparation of Medium: Peel and slice carrots. Add to 1.0L of dis-tilled/deionized...
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Handbook of Microbiological Media, Fourth Edition part 37 pdf

Handbook of Microbiological Media, Fourth Edition part 37 pdf

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... 0.2mL of sterile solution A, 0.2mL of sterile solutionB, and 0.1mL of sterile solution C for each 10.0mL of medium. Mixthoroughly. Use immediately.Use: For the cultivation and maintenance of ... intensity of 300–500 lux. For heavy cell suspension supplementperiodically with sterile yeast extract solution to yield a final concen-tration of 0.1%.Use: For the growth and maintenance of Chloroflexus ... isolation of Neisseria spp. and Haemophilus spp. from avariety of clinical specimens.Chocolate No. 2 Agar Base with HemoglobinComposition per liter:Agar 12.0gHemoglobin 10.0gPancreatic digest of...
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Handbook of Microbiological Media, Fourth Edition part 64 pdf

Handbook of Microbiological Media, Fourth Edition part 64 pdf

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... remove a sufficient amount of medium to permit the addition of feeding medium. Add 1.0mL of feeding solution per each 100.0mL of medium.Use: For the isolation and cultivation of Ectothiorhodospira ... to 25°C. Aseptically add 10.0mL of ster-ile MgCl2·6H2O solution, 6.0mL of sterile Na2S·9H2O solution, 5.0mL of sterile CaCl2·2H2O solution, and 4.0mL of sterile NH4Cl solution.Mix ... to 25°C. Aseptically add 10.0mL of ster-ile MgCl2·6H2O solution, 6.0mL of sterile Na2S·9H2O solution, 5.0mL of sterile CaCl2·2H2O solution, and 4.0mL of sterile NH4Cl solution.Mix...
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Handbook of Microbiological Media, Fourth Edition part 68 pdf

Handbook of Microbiological Media, Fourth Edition part 68 pdf

Điện - Điện tử

... 1.0gPreparation of Rosolic Acid Solution: Add rosolic acid to 0.2NNaOH and bring volume to 100.0L. Mix thoroughly. Preparation of Medium: Add 10.0mL of rosolic acid solution to950.0mL of distilled/deionized ... 1.0gPreparation of Rosolic Acid Solution: Add rosolic acid to 0.2NNaOH and bring volume to 100.0L. Mix thoroughly. Preparation of Medium: Add 10.0mL of rosolic acid solution to950.0mL of distilled/deionized ... 99.0mLPreparation of Vitamin K1 Solution: Add vitamin K1 to 99.0mL of absolute ethanol. Mix thoroughly. Hemin Solution:Composition per 100.0mL:Hemin 1.0gNaOH (1N solution) 20.0mLPreparation of Hemin...
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Handbook of Microbiological Media, Fourth Edition part 86 pdf

Handbook of Microbiological Media, Fourth Edition part 86 pdf

Điện - Điện tử

... rubberstoppers under a gas phase of 80% H2 + 20% CO2 (300 kPa).Use: For the cultivation of Persephonella hydrogeniphila and Hydro-genivirga caldilitoris.Hofer’s Alkaline MediumComposition ... separate flask, add potato starch to 50.0mL of distilled/deionized water. Add the starch solution to 450.0mL of boil-ing distilled/deionized water. Add 10.0mL of solution 8 and the agar.Mix thoroughly. ... 5.7gMnCl2·6H2O 0.8gNaMoO4·2H2O 0.15gHCl, concentrated 1.0mLPreparation of Solution 2: Add the 1.0mL of concentrated HCl to100.0mL of distilled water in a 1.0L volumetric flask. Dissolve eachcomponent...
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Handbook of Microbiological Media, Fourth Edition part 105 pdf

Handbook of Microbiological Media, Fourth Edition part 105 pdf

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... 100.0mL of sterile phenol solu-tion and 1.0mL of sterile trace elements solution SL-4. Mix thoroughly.Aseptically distribute into sterile tubes or flasks.Use: For the cultivation of Bacillus ... Aseptically combine 500.0mL of solu-tion A and 500.0mL of solution B. Mix thoroughly. Pour into sterilePetri dishes or distribute into sterile tubes.Use: For the cultivation of aciduric, thermophilic ... into sterile tubes. Use: Recommended by the Directive of the Council of the EuropeanUnion 98/83/EC for the isolation and enumeration of Clostridium per-fringens from water samples using the...
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Handbook of Microbiological Media, Fourth Edition part 126 pdf

Handbook of Microbiological Media, Fourth Edition part 126 pdf

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... aseptically and anaerobi-cally add 1.0mL of filter-sterilized methanol and 1.0-2.0mL of methane-thiol gas to each liter of medium. Addition of 10-20mg of sodiumdithionite per liter (e.g., from ... tubes.Use: For the isolation of pathogenic Neisseria species. For antimicro-bial susceptibility testing of a variety of bacterial species. For the cul-tivation and maintenance of Moraxella osloensis ... 0.2gPreparation of Selective Supplement Solution B: Add 0.2g of 2,3,5,triphenyltetrazolium chloride to distilled/deionized water andbring volume to 20.0mL. Mix thoroughly. Filter sterilize.Preparation of...
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Handbook of Microbiological Media, Fourth Edition part 129 pdf

Handbook of Microbiological Media, Fourth Edition part 129 pdf

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... 4.0mgPreparation of Page’s Amoeba Saline: Add components to dis-tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Preparation of Medium: Add the glucose and ox liver digest to1.0L of Page’s ... Autoclave for 15 minat 15 psi pressure–121°C. Aseptically add 100.0mL of sterile solutionA, 3.0mL of sterile solution B, and 1.0mL of sterile trace elements so-lution SL-4. Mix thoroughly. Aseptically ... 6.5. Pour into sterile Petri dishes orleave in tubes.Use: For the maintenance of stock cultures of a variety of microorgan-isms. Neopeptone Glucose Rose Bengal Aureomycin® AgarComposition...
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Handbook of Microbiological Media, Fourth Edition part 131 pdf

Handbook of Microbiological Media, Fourth Edition part 131 pdf

Điện - Điện tử

... 0.5mLPreparation of VFA Solution: Add volatile fatty acids to 98.0mL of KOH solution. Mix thoroughly. Filter sterilize. Store at 4°C.Preparation of Medium: Combine 20.0mL of NaHCO3 solution,20.0mL of rabbit ... 4.0mgPreparation of Chelated Metals Solution: Add components todistilled/deionized water and bring volume to 1.0L. Mix thoroughly.Filter sterilize. Preparation of Medium: Adjust pH of 1.0L of seawater ... 0.5mLPreparation of VFA Solution: Add volatile fatty acids to 98.0mL of NaOH solution. Mix thoroughly. Filter sterilize. Store at 4°C.Preparation of Medium: Open the flask containing 94.0mL of cooled...
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Handbook of Microbiological Media, Fourth Edition part 138 pdf

Handbook of Microbiological Media, Fourth Edition part 138 pdf

Điện - Điện tử

... add 0.5mL of sterile glucose solution, 0.1mL of sterile pancreaticdigest of casein solution, 0.1mL of sterile yeast extract solution, 0.1mL of sterile Na2S·9H2O solution, and 0.1mL of sterile ... Solution:Composition per 10.0mL:Pancreatic digest of casein 1.0gPreparation of Pancreatic Digest of Casein Solution: Addpancreatic digest of casein to distilled/deionized water and bring vol-ume ... maintenance of Acinetobacter lwoffii.Peptone Yeast Extract Medium(ATCC Medium 1366)Composition per liter:Peptone 10.0gNaCl 5.0gYeast extract 5.0gpH 7.2 ± 0.2 at 25°CPreparation of Medium:...
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Handbook of Microbiological Media, Fourth Edition part 155 pdf

Handbook of Microbiological Media, Fourth Edition part 155 pdf

Điện - Điện tử

... Aseptically and anaerobi-cally add 50.0mL of sterile sodium pyruvate solution, 20.0mL of sterileNaHCO3 solution, 10.0mL of seven vitamin solution, and 10.0mL of sterile Na2S·9H2O solution. Mix ... Aseptically and anaerobi-cally add 50.0mL of sterile sodium crotonate solution, 20.0mL of sterile NaHCO3 solution, 10.0mL of seven vitamin solution, and10.0mL of sterile Na2S·9H2O solution. Mix ... identification of Salmonella species and differentiation of Salmonella spp. other organisms in the family Enterobacteriaceae.This medium combines two chromogens for the detection of Salmo-nella...
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