... earlier editions. Part I of this edition, a
Handbookmainstay, coversimportant currentprofessionaland scientificissues,but manyofthe topicsin
this edition arenew and reflect importantemerging professional ... xi
Preface
This editionof theHandbook of Counseling Psychology,like all three prior editions, has three primary
objectives: (1) to provide a scholarly review of important areas of counseling psychology ... have
completed this editionof theHandbookwithout them. Finally, we thank each other. Although a bit
out of the ordinary for an acknowledgment, we’ve had, and continue to have, a heck of a professional
run...
... high levels of amino acids. It contains total nitrogen of
11.9% and NaCl of 1.4%.
Liver Digest Neutralized
A papaic digest of liver that contains total nitrogen of 11.0%
and NaCl of 1.6%.
Mycological ... digest of soybean meal. It has a high vitamin and a high
carbohydrate content.
Polypeptone™ Peptone
A mixture of peptones composed of equal parts of pancreatic
digest of casein and peptic digest of ... the numbers of particular species of
microorganisms. Most often, mixtures of growth factors are used in
microbiological media. Acid hydrolysates of casein commonly are used
as sources of amino acids....
... for the
standard methods examination of water and food.
Sources of Media
The Handbookof Microbiological Media includes the media produced
by major suppliers of dehydrated media, including Oxoid ... comparison of a new chromagen
medium, MRSASelect, to CHROMagar MRSA and mannitol-salt
medium with oxacillin or cefoxitin for detection of methicillin-resis-
tant Staphylococcus aureus. Journal ofClinical ... aseptically dilute 200.0mL of 5× stock
solution with 789.0mL of sterile distilled/deionized water. Aseptically
add 10.0mL of sterile carbon source solution and 1.0mL of sterile
MgSO
4
·7H
2
O...
... tubes.
Use: For the maintenance of cultures of Escherichia coli and Staphy-
lococcus aureus. For the detection of antibacterial activity of fabrics.
Test cultures of Escherichia coli or Staphylococcus ... Prior to inoculation of cultures, aseptically and
anaerobically add 0.1mL of sterile reducing agent solution and 0.5mL
of sterile glucose solution to each tube containing 9.4mL of sterile bas-
al ... the resistance of textiles to fungi that cause mildew
and rot. Also used to test the effectiveness of fungicides used on tex-
tiles for preventing the growth of fungi. Cultures of Chaetomium glo-
bosum...
... Acid: Filter sterilize 40.0mL of acetic acid
using a teflon filter.
Preparation of Ethanol: Filter sterilize 30.0mL of ethanol using a
teflon filter.
Preparation of Medium: Add components, except ... anaerobically add 0.1mL of sterile
glucose, 0.1mL of sterile
L-cysteine·HCl·H
2
O solution, and 0.1mL of
sterile Na
2
S·9H
2
O solution to each tube. Mix thoroughly.
Use: For the cultivation of Acetitomaculum ... autoclav-
ing, the pH of the medium will be 7.2. Prior to inoculation of cultures,
aseptically and anaerobically add 0.1mL of sterile reducing agent solu-
tion and 0.5mL of sterile cellobiose...
... add 5.0mL of sterile ferric citrate solution
and 1.0mL of sterile trace elements solution. Mix thoroughly. Asepti-
cally distribute into sterile tubes or flasks.
Use: For the cultivation of Rhodopseudomonas ... inoculation, aseptically and anaerobically
add 0.25–0.50mL of neutralized sulfide solution.
Use: For the cultivation and maintenance of members of the family
Rhodospirillaceae, including Rhodomicrobium ... 10.0mL:
L-Cysteine 0.1g
Preparation of L-Cysteine Solution: Add 0.1g of L-cysteine to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components,...
... for 15 min at 15 psi
pressure–121°C. Aseptically add 0.6mL of sterile raffinose solution,
0.2mL of sterile Na
2
CO
3
solution, and 0.2mL of sterile CoCl
2
·6H
2
O
solution to each tube. Mix thoroughly. ... of Acid Fuchsin Solution: Add Acid Fuchsin to
50.0mL of 5% aqueous dioxan. Mix well to dissolve.
Caution: Acid Fuchsin is a potential carcinogen and care must be tak-
en to avoid inhalation of ... 0.02g
Preparation of Bromcresol Purple Solution: Add Bromcresol
Purple to distilled/deionized water and bring volume to 20.0mL. Mix
thoroughly. Filter sterilize.
Preparation of Medium: Add agar to 500.0mL of...
... 10.0mL of CaCl
2
solution, 10.0mL of MgSO
4
·7H
2
O solution,
10.0mL of NaNO
3
solution, 10.0mL of K
2
HPO
4
solution, 10.0mL of
KH
2
PO
4
solution, and 10.0mL of NaCl solution to distilled/deionized
water ... 1.0g
Preparation of FeCl
3
Solution: Add FeCl
3
to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly.
Preparation of Bristol's Solution: Add 10.0mL of NaNO
3
solu-
tion, 10.0mL of ... psi pressure–121°C. Cool to
50°–55°C.
Preparation of Medium: Aseptically combine 500.0mL of solu-
tion A, 1.0mL of solution B, and 500.0mL of solution C. Mix thor-
oughly. Pour into sterile...
... of Medium: Aseptically combine 900.0mL of solu-
tion A with 100.0mL of solution B. Mix thoroughly. Pour into sterile
Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of ... elements solution 20.0mL
Preparation of Base Solution: Sparge 2.0L of distilled/deionized
water with 100% N
2
to remove O
2
. Add components to 2000.0mL of O
2
-
free distilled/deionized water. ... Aseptically combine 500.0mL of solu-
tion A with 500.0mL of solution B. Mix thoroughly. Bring pH to 9.5.
Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Natronobacterium...
... 5.2g
Preparation of A & A FeEDTA Solution: Dissolve 5.2g of KOH
in 186.0mL of distilled/deionized water. Add 20.4g of disodium
EDTA·2H
2
O. Add 13.7g of FeSO4·7H2O to 364.0mL of distilled/deion-
ized ... pressure–121°C.
Preparation of Medium: Saturate cooled solution A under 100%
CO
2
at 0.05–0.1 atm pressure for 30 min with magnetic stirring. Add
860.0mL of solution B, 5.0mL of solution C, 5.0mL of solution ... iron in the
medium will precipitate out of solution as black flocs.
Use: For the cultivation and maintenance of Amphibacillus xylanus,
Amoebobacter pedioformis, and Amoebobacter purpureus.
Amphibacillus...
... anaerobi-
cally add 20.0mL of sterile glucose solution. Aseptically and
anaerobically distribute into sterile tubes or bottles.
Use: For the cultivation of Anaerofilum agile and Anaerofilum pentos-
ovorans. ... dishes or leave in tubes.
Use: For the cultivation of a variety of anaerobic microorganisms.
With added blood for the detection of hemolytic activity of clostridia,
streptococci, and other anaerobic ... dishes or leave in tubes.
Use: For the cultivation of a variety of anaerobic microorganisms.
With added blood for the detection of hemolytic activity of clostridia,
streptococci, and other anaerobic...
... allowing for specific isolation of
these three Bacillus species. Inclusion of inhibitory compounds into
the medium prevents the growth of environmental contaminants. The
use of proprietary chromogenic ... Cool to 45°–50°C. Aseptically add 25.0mL of sterile
glucose solution, 10.0mL of sterile CaCl
2
solution, 10.0mL of sterile
MgSO
4
solution, and 5.0mL of sterile anthranilic acid solution. Mix
thoroughly. ... to avoid inhalation of the powdered dye and
contact with the skin.
Use: For the determination of carbohydrate fermentation reactions of
microorganisms, particularly members of the Enterobacteriaceae....