Beta cardiotoxin a novel protein isolated from the venom of ophiophagus hannah (king cobra) showing beta blocker activity

... Lalitha Rajagopalan, my brothers Mr Prasanna and Mr Vasanth and my cousins Ms Aarthi Ravichandran and Ms Madhulika Ravichandran Nandhakishore Rajagopalan January, 2008 v TABLE OF CONTENTS Page ... and highly neurotoxic 1.1.2 Ophiophagus hannah This thesis deals with proteins isolated from the venom of Ophiophagus hannah (King cobra) This snake belongs to the family elapidae and it is the ... Elapidae is a diverse family of venomous snakes found in Americas, Asia, Africa and Australia (Figure 1.2 D) This family includes cobras, kraits, mambas, coral snakes and a large diversity of Australian...

A novel protein isolated from the venom of ophiophagus hannah (king cobra) showing beta blocker activity

Structural and functional characterization of haditoxin, a novel neurotoxin isolated from the venom of ophiohagus hannah (king cobra

... β-cardiotoxin, an all β-sheet protein isolated from the venom of Ophiophagus hannah (king cobra) Manuscript under preparation xvi (5) Roy A, Sivaraman J, and Kini RM Structural and functional characterization ... forests and mangrove swamps in parts of Southeast Asia, South China and India Chapter One A B C Figure 1.1: Ophiophagus hannah (king cobra) and its geographical distribution (A) An adult king cobra ... hypotensive and vasorelaxant properties Isolated from the venom of Atractaspis engaddensis These isopeptides are structurally and functionally related to mammalian endothelins They are potent vacoconstrictors...

Báo cáo khoa học: Phaiodotoxin, a novel structural class of insect-toxin isolated from the venom of the Mexican scorpion Anuroctonus phaiodactylus pdf

... synthesized from poly (A) + mRNA using M-MLV reverse transcriptase The cDNA was joined with the adaptor provided by the kit (5¢-gcugauggcgaugaaugaacacugcguuugCUGG CUUUGAUGAAA-3¢) using T4 DNA ligase ... cloning of phaiodotoxin Total RNA was isolated from venomous glands situated at the last postabdominal segment (telson) of one Anuroctonus phaiodatylus scorpion, by the method of Chirgwin et al [22] ... were analyzed manually and automatically by SEQUEST software The acquisition and deconvolution of data were performed with the XCALIBUR software on a Windows NT PC data system Determination of...

Báo cáo khoa học: A new highly toxic protein isolated from the death cap Amanita phalloides is an L-amino acid oxidase pdf

... it was suggested that amine oxidases are enzymes of cellular amino acid catabolism, comprising potential candidates for a mechanism that catalyses nitrogen mineralization from amino acids at the ... organism In conclusion, in the present study, a novel cytotoxic protein was isolated from the death cap and characterized The physico-chemical, chemical and biological characteristics of this protein ... new L-amino acid oxidase from Daboia russellii siamensis venom Toxicon 54, 763–771 Zhang L & Wu WT (2008) Isolation and characterization of ACTX-6: a cytotoxic l-amino acid oxidase from Agkistrodon...

Báo cáo khoa học: Nautilin-63, a novel acidic glycoprotein from the shell nacre of Nautilus macromphalus doc

... the ASM and the delay of the reaction was dosedependent At approximately 50 lg of nacre ASM, a complete inhibition of the precipitation of calcium carbonate was recorded The observation of the inhibitory ... (‘interlamellar matrix’), at the boundary of adjacent mature nacre tablets (‘intertabular matrix’), or within the crystallites (‘intracrystalline matrix’) First, the interlamellar matrix is assumed ... represented as ngÆlg)1 of the total matrix and as a percentage of the total identiﬁed carbohydrate compounds ND, not detected ngÆlg)1 of matrix (% of total) % of total amino acids Monosaccharide Amino acid...

Báo cáo khoa học: A Kazal prolyl endopeptidase inhibitor isolated from the skin of Phyllomedusa sauvagii pdf

... interaction with their membranes Materials and methods Materials All chemicals were of the purest analytical grade available Proteases, aprotinin, lysozyme, and a- casein were from Sigma-Aldrich ... purication, and refolding Fig Isolation of Phyllomedusa sauvagii Kazal protein and (PSKP-1 and PSKP-2, respectively) from a P sauvagii skin extract (A) FPLC-size exclusion chromatography The bar ... of the anuran, P sauvagii, whose sequence indicates membership of the Kazal family Most members of this family are small protein inhibitors of serine proteases and have a characteristic pattern...

Báo cáo khoa học: A novel carbonic anhydrase from the giant clam Tridacna gigas contains two carbonic anhydrase domains pptx

... CTCAG TACAG TCAAG TACAA TCGAG TTGAA TAAAG GTCAG ttccag ttgtag ttctag ttctag tttcag ttacag ttacag ctacag tttcag tttcag tttcag ttccag a gtaaac gtaggt gtgggt gttagt gtatat gttggg gtatgt gtactt gtactg ... CA domains which are translated into the one peptide, one from the green alga Dunaliella salina contains two a- CA domains while the second from the red alga Porphyridium purpureum contains two ... contain three domains, based upon database searches, a signal sequence (Met1–Ala17) and two domains with homology to the a- CA family, n-tgCA (Ala18–Thr289) and c-tgCA (Ala290–Ser600) (Fig 2) The...

Báo cáo khoa học " Structure elucidation and antioxidant activity of a novel polysaccharide isolated from Tricholoma matsutake " ppt

... T matsutake polysaccharide, named TMP -A, was obtained by the above processes and the yield rate of TMP -A was 0.22% (0.432 g) for the starting material The DPPH− radical scavenging activity of ... recorded on a Varian Unity INOVA 400/45 in D2 O with tetramethylsilane as internal standard All data were presented as means ± standard deviation (SD) of three replications Statistical analyses were ... scavenging activity of TMP -A Fig Superoxide radical scavenging effect of TMP -A from Tricholoma matsutake DPPH is a useful reagent for investigating the free radical scavenging activities of various samples...

Báo cáo y học: "The identification and characterization of a novel protein, c19orf10, in the synovium" docx

... staining (g) Intense staining of a hyperplastic RA synovial lining cell layer This staining was typical of most areas of RA synovium where the lining was hyperplastic (h) An area of an RA synovial ... stained positively (arrow) (e) Intense staining of individual cells in the lining layer of a typical OA synovium (f) An area of an OA synovium demonstrates a lining layer completely devoid of ... synovial lining layer and perivascular regions of RA (OCT section) tissue.(b) Intense staining of the synovial lining layer and perivascular regions of OA (paraffin section) tissue (c) An area demonstrating...

Báo cáo y học: "Anti-inflammatory and arthritic effects of thiacremonone, a novel sulfurcompound isolated from garlic via inhibition of NF-κB" pptx

... radiographic analysis of arthritic hind paws and HJ isolated thiacremonone from garlic and provided SBH participated in data analysis and helped to draft the manuscript All authors read and approved ... 14 Rahman K: Historical perspective on garlic and cardiovascular disease J Nutr 2001, 131:977S-979S Tanaka S, Haruma K, Yoshihara M, Kajiyama G, Kira K, Amagase H, Chayama K: Aged garlic extract ... cyclooxygenase-2 (COX-2) and iNOS As a result, inhibition of signal pathways leading to inactivation of NF-κB is now widely recognized as a valid strategy combating autoimmune, inflammatory, and...

Tài liệu Báo cáo Y học: Antibacterial and antifungal properties of a-helical, cationic peptides in the venom of scorpions from southern Africa pptx

... opistoporins (34%) and pandinin (31%) This means that there is a high conservation in amino acid sequence of the peptides in the venom of scorpions that belong to the same family, independent of the ... ﬁt in the theory that amphipathicity, a-helicity and cationicity due to the presence of high amounts of lysine and arginine are the most important factors for activity and are important for the ... parabutoporin and opistoporin The peptides were prepared by solid-phase synthesis by Ansynth Service B.V (the Netherlands) using the Fmoc/ tert-butyl-based methodology with Rink resin as the solid...

Tài liệu Báo cáo Y học: Trehalose-based oligosaccharides isolated from the cytoplasm of Mycobacterium smegmatis Relation to trehalose-based oligosaccharides attached to lipid docx

... known They could be synthesized in the cytosol by glycosyltransferases which add glucosyl or galactosyl residues to the free trehalose that arises from trehalose phosphate by the action of the speciﬁc ... and isolated from the cytoplasm of M smegmatis, will also be found as part of the cell wall components of this organism All trehalose-containing structures reported from mycobacterial glycolipids ... the 3-hydroxy group of the second glucose of trehalose, has been isolated from the cell wall lipids of Mycobacterium kansasii [1] That study strongly suggests that some of the oligosaccharides described...

Báo cáo khoa học: Novel c-carboxyglutamic acid-containing peptides from the venom of Conus textile docx

... Gla-containing peptides from C textile venom E Czerwiec et al The biosynthesis of Gla is a complex reaction that involves replacement of a proton on the c-carbon of a Glu residue with a CO2 molecule [13] The ... and the mechanistic properties of an ancestral carboxylation system Here, we describe the puriﬁcation and characterization of four novel Gla-containing conotoxins from C textile All of the peptides ... Gla-containing peptides from C textile venom E Czerwiec et al poor substrate for the Conus c-carboxylase, exhibiting a Km of 1.8 mm Addition of amino acids )8 to )1 (a strongly charged part of the precursor)...

Báo cáo khoa học: Puriﬁcation and characterization of three isoforms of chrysophsin, a novel antimicrobial peptide in the gills of the red sea bream, Chrysophrys major doc

... lamellae and EGC-like cells of the primary lamellae of red sea bream gills We are currently trying to obtain cDNAs encoding chrysophsins from the gills of red sea bream, and we aim to investigate the ... (minimal lethal concentration) of native and synthetic chrysophsins compared with that of magainin-2 Minimal lethal concentrations of peptide are given in lM and are the concentration of substance ... Immunohistochemical localization of chrysophsins in the gills of the red sea bream Adjacent sections in the gills were immunostained with chrysophsin-1 antibody (A, C, E and F) and with hematoxylin and eosin...

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