báo cáo khoa học: " Manufacture of IRDye800CW-coupled Fe3O4 nanoparticles and their applications in cell labeling and in vivo imaging" ppt

... IRDye800CW-coupled Fe3O4 nanoparticles and their applications in cell labeling and in vivo imaging. Journal of Nanobiotechnology 2010 8:25.Submit your next manuscript to BioMed Central and take full advantage of: ... 774/789 nm), and investigated their applicability in cell labeling and in vivo imaging.Results and disc ussionsPreparation of IRDy800CW-MNPsM-2, 3-dimercaptosuccinic acid (DMSA) has often beenused ... for imagingcentered at 800 nm, which can be applied to in vitro cell labeling and in vivo imaging.MethodsCells, animals and chemicalsThe RAW264.7 cell line was purchased from the ChinaCenter...

báo cáo khoa học: " Gum arabic modified Fe3O4 nanoparticles cross linked with collagen for isolation of bacteria" pps

... meningitis and urinary tract infections etc. S. aureusshows affinity for a wide range of mammalian plasma and extracellular matrix proteins. Among the proteins, col-lagen was estimated to bind ... pattern similarities of Fe3O4 and Fe2O3, the survey XPS spectrum together with spectra of Fe (2p3/2,1/2)(insetofFigure2)andO(1s)(insetofFigure 2) is collected and shown in Figure 2. Themeasured ... formation of m agnetite [30,31]. In addition, the peaks corresponding to sodium, nitrogen,chlorine and carbon originate from PDADMAC indicat-ing the existence of PDADMAC on the surface of ironoxide....

Báo cáo khoa học: "Effect of intern’s consecutive work hours on safety, medical education and professionalism"

... performance of upper-levelresidents and other medical staff across a variety of disciplines. We likewise agree that optimizing patient hand-offs, medical education, and trainees’ sense of professionalism ... rapidly increases after 16 hours of sustainedwakefulness, a finding reflected in the twofold increase in interns’ attentional failures after they had been working formore than 16 hours on the ... Journal Club review [1] of their studies examining the effect of reducing interns work hours in the intensive care unit [2,3].Our statement that “interns in the traditional arm may havehad more...

Tài liệu Báo cáo khoa học: Proteolysis of Pseudomonas exotoxin A within hepatic endosomes by cathepsins B and D produces fragments displaying in vitro ADP-ribosylating and apoptotic effects doc

... min postinjec-tion), suggesting a high binding capacity of ETA in hepatic parenchyma. Indeed, injection of the toxin intomice has been shown to result in an early and pro-found inhibition of ... cathepsins B and D, with themajor cleavages generated in vitro occurring mainly within domain III of ETA-A. Cell- free endosomes preloaded in vivo with ETA intraluminallyprocessed and extraluminally ... timeactivation of the intrinsic apoptotic pathway with ETAcytotoxicity in a physiological state.Results In vivo endocytosis and metabolic fate of ETA in rat liverThe kinetics of in vivo uptake of ETA...

Tài liệu Báo cáo khoa học: Phosphorylation of cyclin dependent kinase 4 on tyrosine 17 is mediated by Src family kinases pptx

... chromosomalaberrations and cell death, indicating that phosphory-lation of this site is important for integrity of the G1checkpoint [13]. Phosphorylation of CDK4 on Y17has also been detected in cells entering ... the tyrosine 17 kinaseactivity in HeLa lysates. C-YES also phosphorylated cyclin dependentkinase 4 when transfected into HCT116 cells, and treatment of cells withSrc family kinase inhibitors ... mechanisms, including phosphorylation of tyrosine 17. This site is equivalent to tyrosine 15 of cyclin dependentkinase 1, which undergoes inhibitory phosphorylation by WEE1 and MYT1; however, the kinases...

Tài liệu Báo cáo khoa học: Control of the coagulation system by serpins Getting by with a little help from glycosaminoglycans pptx

... aprotease inhibition profile for numerous ‘arginine-specific’ serine proteases, including trypsin, thrombin (in the absence and presence of thrombomodulin), acti-vated protein C, acrosin, kallikrein, ... activate the serpinby displacing the acidic amino terminus from an intra-molecular interaction with the basic GAG binding site and freeing it for binding to the thrombin anion-bind-ing exosite-1 ... sequence-specific binding of heparin ⁄ heparansulfate to PCI. In a cell- derived series of studies,heparan sulfate-containing proteoglycans wereinvolved in binding to PCI using cultured humanepithelial...

Báo cáo khoa học: Characterization of the rice carotenoid cleavage dioxygenase 1 reveals a novel route for geranial biosynthesis ppt

... control of an arabinose-induciblepromoter. Overnight cultures (2.5 mL) were inoculated into50 mL of 2 · YT medium containing 0.2% (w ⁄ v) arabinose,grown at 28 °CtoaD600 nm of 0.7, and induced ... 739temperature of 340 °C, which was maintained for an addi-tional 5 min. The He carrier gas ﬂow was maintained at1mLÆmin)1using a split ﬂow of 1 : 20. The splitless timewas 3 min, and the injector ... converted into pseudoionone (3) and three dialdehydes of different chain lengths (1, 4 and 5 from II; 4, 6 and 7 from III), asindicated by their UV ⁄ visible-spectra (shown in the insets) and retention...

Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx

... could maintain a small accessible region in theglobin locus during the differentiation of hematopoi-etic stem cells to erythroid cells. Transcription of HS3 could be important in maintaining this ... Thesequential silencing of genes involved in maintenance of pluripotent and multipotent states and the activa-tion of those involved in differentiation is believed tobe a dominant factor in the progression ... examine the developmental progres-sion of cell samples and in all cases except that of theRex-1 gene, primer sets span introns. Day 0 cells arecomposed of ES cells and MEF cells grown in ES...

Báo cáo khóa học: Characterization of Mesorhizobium huakuii lipid A containing both D-galacturonic acid and phosphate residues ppt

... De-O-acylation of lipid AfractionsledtodecayofspeciesZandYandresultedinincrease of signals corresponding to ions of species X(data not shown). The total decrease of mass due to de-O-acylation of phosphorylated ... glucosamines. Lipid A is responsible for theendotoxic properties of lipopolysaccharide. The structure of lipid A seems to be essential in maintaining outer membraneintegrity and flexibility and ... least six species of molecules whose struc-tures differ both in the phosphorylation of sugar backbone and in fatty acylation. The backbone consists of a b (1¢fi 6)linked 2,3-diamino-2,3-dideoxyglucose...

Báo cáo khoa học: Induction of raft-like domains by a myristoylated NAP-22 peptide and its Tyr mutant potx

... N-terminalfragments of NAP-22 present in cells [2]. Furthermore,a construct composed of the N-terminal segment of CAP-23 and containing 40 amino acids arranges in apunctate pattern on the cell ... cationiccluster of amino acid residues and the insertion of hydrophobic amino acids into the membrane. In somecases, the insertion of groups that promote the forma-tion of cholesterol-rich domains will ... concentration of 20–40 lm [40].Thus, there are comparable amounts of PtnIns(4,5)P2 and NAP-22 in the cell and the ratio is within therange used in our work. Since NAP-22 binds toPtnIns(4,5)P2...

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