... BD(Franklin Lakes, NJ, USA). Ammonium sulphate and aminoacids were from Merck (Darmstadt, Germany).Strains, plasmids and primersThe strains and plasmids used are listed in Table 1. PlasmidpFA 6a- HIS3MX6 ... regulated by the mito-gen-activated protein (MAP) kinase and mTOR-signal-ling pathways [17]. These signalling pathways activatethe eEF2 kinase in response to mitogens and otherstimuli that increase ... cerevisiae(NP_014776), Saccharomyces castellii (AAO32487), Saccharomyceskluyveri (AAO32562), Glugea plecoglossi (BAA11470), Ashbya gos-sypii (AAS53513), Candida albicans (CAA70857), Schizosaccharomy-ces...