Báo cáo khoa học: Cloning and functional characterization of Phaeodactylum tricornutum front-end desaturases involved in eicosapentaenoic acid biosynthesis doc
Báo cáo khoa học: Cloning and functional characterization of Phaeodactylum tricornutum front-end desaturases involved in eicosapentaenoic acid biosynthesis doc
... genes encoding fatty acid desaturases involved in eicosapentaenoic acid biosynthesis. Using a combination of PCR, mass sequencing and library screening, the codingsequences of two desaturases ... Cloning and functional characterization of Phaeodactylum tricornutum front-end desaturases involved in eicosapentaenoic acid biosynthesis Fre´de´ric Domergue1,*, ... organism for cloning the genes encodingthe different fatty acid desaturases involved in EPA biosynthesis and identified two sequences coding for fatty acid desaturases with D5- and D6-regioselectivities....
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Báo cáo khoa học: Cloning and functional characterization of Arabidopsis thaliana D-amino acid aminotransferase – D-aspartate behavior during germination pdf
... d-enantiomers, acting on a variety of d-amino acids, including d-isomers of branched aminoacids (Fig. 6). The amino acid residues presumed to be involved in substrate recognition and binding are notconserved ... cloning and functional characterization of a d-amino acid aminotransferase in eukaryotes. The results presented here provide important information forunderstanding the significance of d-amino acids in ... d-amino acid aminotransferase, which is presum-ably involved in the metabolism of d-Asp in the plant by catalyzing trans-amination between d-amino acids. This is the first report of cDNA cloning and...
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Tài liệu Báo cáo khoa học: Molecular and functional characterization of adenylate kinase 2 gene from Leishmania donovani pdf
... presence of three distinct domains: the rigidCORE-domain and two smaller peripheral domains ormobile parts, the NMP-binding site and the LID-domain[4]. The NMP-binding site has many intermolecularcontacts ... fromthe amino acid sequence of AK2 protein. The purification and functional folding of L. donovani AK from the E. colicell lysates was carried out by washing and dissolving theinclusion bodies in 8Murea. ... wereobtained by simple rotation of the same model. The flexible LIDdomain appears at top of the three figures, NMP-binding site and ATPbinding domain are displayed, NH2- and COOH-termini are alsoindicated.Ó...
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Báo cáo khoa học: Molecular and functional characterization of novel CRFR1 isoforms from the skin pptx
... 2004Molecular and functional characterization of novel CRFR1 isoformsfrom the skinAlexander Pisarchik and Andrzej SlominskiDepartment of Pathology and Laboratory Medicine, University of Tennessee ... exception of an artificial isoform (CRFR1h2) with theinsertion of 37 amino acids between the ligand bindingdomain and the first extracellular loop that was capable of producing detectable levels of ... equalvolume of DMEM media containing 10% fetal bovineserum was added and cells were incubated overnight. Nextmorning, the cells were washed by DMEM and incubated in DMEM media containing 5% fetal bovine...
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Báo cáo khoa học: Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis docx
... identified a proteincontaining a single ankyrin repeat that interacts withHIV-1 viral protein R (Vpr) and we designated thisprotein as Vpr-binding ankyrin repeat protein(VBARP). This interaction ... ankyrin repeat motif-containing proteins play an important role in protein–protein interactions. ANKHD1 proteins are known to possessmultiple ankyrin repeat domains and a single KH domain with ... Institute, Shanghai, China.8 Smith RK, Carroll PM, Allard JD & Simon MA (2000)MASK, a large ankyrin repeat and KH domain-con-taining protein involved in Drosophila receptor tyrosinekinase...
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Báo cáo khoa học: Structural and functional characterization of human Iba proteins ppt
... (B,C)Actin polymers in the presence of Iba1 (B) and Iba2 (C). (D,E) For comparison, actin polymers are shown in the presence of the actin cross-linking protein neurabin-2 (D) and an actin binding ... reveals functional simi-larities and differences between Iba1 and Iba2. Weinvestigated Ca2+binding and homodimerization of Iba1 and Iba2. Furthermore, F-actin binding and cross-linking assays ... Ca2+coordination.Sedimentation experiments and microscopy detected pronounced, indistin-guishable F-actin binding and cross-linking activity of Iba1 and Iba2 withinduction of F-actin bundles....
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Báo cáo khoa học: Cloning and functional analysis of 5¢-upstream region of the Pokemon gene pptx
... were maintained in RPMI-1640 medium con-taining 10% fetal bovine serum (Invitrogen). All these cellswere incubated in a humidified 5% CO2incubator at37 °C.Creation of deletion constructs of ... Protein Engineering, Beijing Institute of Biotechnology, ChinaThe poxvirus and zinc finger (POZ) domain, formerlytermed the broad complex, tramtrack and bric-a-brac(BTB) domain, was initially ... transfected with of 30 ng of the pRL-TKvector and 300 ng of the pGL3 vectors containing differentlengths of the Pokemon promoter fragment to each well byusing LipofectamineTM2000 (Invitrogen)....
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Báo cáo khoa học: Expression and functional characterization of P2Y1 and P2Y12 nucleotide receptors in long-term serum-deprived glioma C6 cells ppt
... and P2Y1 and P2Y12receptor protein expression and functional activity.Examination of the effects of specific pharmacologicalagents (a single agonist and two specific antagonists of P2Y1 and ... can induce cell cycle arrest in many cell lines, including astrocytes [26,27]. In the pre-sent study, we used propidium iodide staining and flowABCDEFGHIFig. 1. Induction and reversal of a ... results again high-light the stimulatory role of P2Y12 in mitogen-activatedprotein kinase and Akt kinase signaling pathways.On the other hand, the functional activity of theP2Y1receptor in long-term...
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Báo cáo khoa học: Structural and functional aspects of unique type IV secretory components in the Helicobacter pylori cag-pathogenicity island potx
... moleculeinvolving a-helix A, the nearby loop, the first and lastturns of helices E and F, and the C-terminus helices I and J. In addition, there is a lysine-rich N- and C-ter-minus, in accordance ... pylori strains harbouring a func-tional cagPAI, is the activation of nuclear transcriptionfactor-jB and the induction of pro -in ammatory pro-duction of cytokines, mainly interleukin (IL)-8 ... protein or toxin co-regulated pilus biosynthesis protein D has been detected [32]. Yeasttwo-hybrid screens, as noted elsewhere, indicate thatCagG is involved in multiple protein–protein interac-tions...
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Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx
... PRTFDC1 and hypoxanthine. (B) HPRT and hypoxanthine. (C) PRTFDC1 and guanine. (D) HPRT and guanine.Table 2. Km and Vmaxvalues for Hx and G in the presence of 1 mM PRPP, determined using the ... activity of PRTFDC1towards hypoxanthine and guanine is only 0.26% and 0.09%, respectively, of the activity of HPRT, the role of the PRTFDC1 in purine metabolism remainsunclear and it is uncertain ... However, a tight binding of N7 of the purine base and Asp137 was observed in HPRT in complex with ImmGP, indicating a role in transitionstate stabilization during catalysis [17].A series of mutations...
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Từ khóa: báo cáo khoa học mẫubáo cáo khoa học y họcbáo cáo khoa học sinh họcbáo cáo khoa học nông nghiệpbáo cáo khoa học lâm nghiệpBáo cáo quy trình mua hàng CT CP Công Nghệ NPVNghiên cứu sự hình thành lớp bảo vệ và khả năng chống ăn mòn của thép bền thời tiết trong điều kiện khí hậu nhiệt đới việt namNghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiNghiên cứu tổ hợp chất chỉ điểm sinh học vWF, VCAM 1, MCP 1, d dimer trong chẩn đoán và tiên lượng nhồi máu não cấpNghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzđề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDENghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chếSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXQuản lý nợ xấu tại Agribank chi nhánh huyện Phù Yên, tỉnh Sơn La (Luận văn thạc sĩ)BT Tieng anh 6 UNIT 2Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtchuong 1 tong quan quan tri rui roNguyên tắc phân hóa trách nhiệm hình sự đối với người dưới 18 tuổi phạm tội trong pháp luật hình sự Việt Nam (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtTrách nhiệm của người sử dụng lao động đối với lao động nữ theo pháp luật lao động Việt Nam từ thực tiễn các khu công nghiệp tại thành phố Hồ Chí Minh (Luận văn thạc sĩ)Chiến lược marketing tại ngân hàng Agribank chi nhánh Sài Gòn từ 2013-2015