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Báo cáo khoa học: Purification of three aminotransferases from Hydrogenobacter thermophilusTK-6 – novel types of alanine or glycine aminotransferase Enzymes and catalysis pot

Báo cáo khoa học: UDP-galactose 4-epimerase from Kluyveromyces fragilis – catalytic sites of the homodimeric enzyme are functional and regulated docx

Báo cáo khoa học: UDP-galactose 4-epimerase from Kluyveromyces fragilis catalytic sites of the homodimeric enzyme are functional and regulated docx

... Linewe-aver–Burk plots with 0–0 .05 mM and 0. 2–0 .35 mM substrate. Units of the ordinate and abscissa are lmolÆmin)1 and mM)1, respec-tively; values correspond to the original plot. Derived values of ... bound as isolated in E1 or modification of the inhibitor-binding site. A corresponding conforma-tional change at the cofactor-binding site was moni-tored from coenzyme fluorescence. There was an ... (A, B)Lineweaver–Burk plots with 0–0 .05 mM and 0.0 5–0 .35 mM sub-strate. Units of the ordinate and abscissa are lmolÆmin)1 and mM)1, respectively; values correspond to the original plot....
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Báo cáo khoa học: A new paradigm for oxygen binding involving two types of ab contacts docx

Báo cáo khoa học: A new paradigm for oxygen binding involving two types of ab contacts docx

... [ 2–6 ]), however, it becomes apparent that theligands ) including O2andCOtotheferrousformandH2O, OH ,N3 and CN to the ferric form ) cannot gainaccess to the closed haem pockets of ... pH 7.0 and 30 °C, indeed, Tyuma et al. [17] reported the P50values of 1.00 Torr for the a chains and 0.45 Torr for the b chains,whereas HbA showed P50¼ 16.59 Torr in the absence of DPG.Certainly, ... H helices and the GH corner, and theother is the a1b2(ora2b1) contact involving mainly helicesC and G and the FG corner [3,7]. When HbA goes from thedeoxy to the oxy form, the a1b2anda2b1 contacts...
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Báo cáo khoa học: Examining multiprotein signaling complexes from all angles The use of complementary techniques to characterize complex formation at the adapter protein, linker for activation of T cells pdf

Báo cáo khoa học: Examining multiprotein signaling complexes from all angles The use of complementary techniques to characterize complex formation at the adapter protein, linker for activation of T cells pdf

... thetransfer of energy from an excited donor fluorophoredirectly to an acceptor fluorophore, leading to anincreased fluorescence emission of the acceptor and aquenching of the emission fluorescence of the ... the donor[5 2–5 4]. For FRET to occur, the donor and acceptormust have a sufficient spectral overlap, a favorable ori-entation and a separation of 1–1 0 nm [52]. Because of the ability of FRET ... titration calorimetry and fluorescence polarization, the affinity of Gads for boththe short 10 amino acid core-binding motif and thecomplete proline-rich region of SLP-76 was extremelystrong and, in...
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Báo cáo khoa học:

Báo cáo khoa học: "Are These Documents Written from Different Perspectives? A Test of Different Perspectives Based On Statistical Distribution Divergence" ppt

... the sharper the test of different perspec-tives.To account for large variation in the number of words and vocabulary size across corpora, we nor-malize the total number of words in a documentcollection ... number of documents |D|, averagedocument length¯|d| , and vocabulary size V of the three corpora.The first perspective corpus consists of arti-cles published on the bitterlemons website3 from late ... Reuters-215786is one of themost common testbeds for text categorization.Each document belongs to none, one, or more of the 135 categories (e.g., “Mergers” and “U.S. Dol-lars”.) The number of documents...
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Báo cáo khoa học: Purification of three aminotransferases from Hydrogenobacter thermophilusTK-6 – novel types of alanine or glycine aminotransferase Enzymes and catalysis pot

Báo cáo khoa học: Purification of three aminotransferases from Hydrogenobacter thermophilusTK-6 novel types of alanine or glycine aminotransferase Enzymes and catalysis pot

... Authors Journal compilation ª 2010 FEBS 1885Purification of three aminotransferases from Hydrogenobacter thermophilus TK-6 novel types of alanine or glycine aminotransferase Enzymes and catalysis Masafumi ... the first report of the primary structure of bacterial gluta-mate:glyoxylate aminotransferase and alanine: glyoxylate aminotransferase, and demonstrates the presence of novel types of aminotransferase ... aminotransferases. Purification and phylogenetic analysis of aminotransferases Enzymes that exhibited GOT, GPT, GGT or AGTactivity were subjected to purification, and three enzymes (AT1, AT2 and...
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Tài liệu Báo cáo khoa học: Purification and sequence identification of anserinase ppt

Tài liệu Báo cáo khoa học: Purification and sequence identification of anserinase ppt

... volume of 50 lL containing0.75 lL of a template, 150 pmol of a forward primer (A),150 pmol of a reverse primer (B or C), 1 · G-Taq buffer,10 nmol each of dATP, dGTP, dCTP and dTTP, and 0.5 ... amino-acidsequences for designing degenerate primers for PCRamplification (data not shown).Cloning of anserinase cDNAPCR was performed using a set of primers (A and B) and the first-strand cDNA for 3¢ rapid ... bp of 5¢ UTR, 1482 bp of ORF, and 299 bp of 3¢ UTR. The coding region of thesequence was translated into 494 amino acids, whichincluded a typical signal peptide of 18 amino acids and two potential...
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Báo cáo khoa học: Covalent and three-dimensional structure of the cyclodextrinase from Flavobacterium sp. no. 92 pdf

Báo cáo khoa học: Covalent and three-dimensional structure of the cyclodextrinase from Flavobacterium sp. no. 92 pdf

... factor of 18.8% and an Rfree of 22.3%with over 90% of the residues in the most favored region of a Ramachandran plot (Table 2). The resulting model isdepicted in Fig. 2. The Ca backbones of ... [38] and in the CD-degrading TVA-II [13], but not in the CD-degrading enzymes ThMA and BaCD nor in the majority of a-amylases. Ca-II is located inthe loop between a1anda2 of domain A (Fig. 2) and Table ... structure. It wouldenable Phe274 of CDase (or Phe286 of TVA-II) to rotatearound its Ca–Cb bond and enter the CD hollow, as hasbeen implied for TVA-II [10] and for Tyr195 of CGTase[49]. Crystal experiments...
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Tài liệu Báo cáo khoa học: EGF receptor in relation to tumor development: molecular basis of responsiveness of cancer cells to EGFR-targeting tyrosine kinase inhibitors docx

Tài liệu Báo cáo khoa học: EGF receptor in relation to tumor development: molecular basis of responsiveness of cancer cells to EGFR-targeting tyrosine kinase inhibitors docx

... of the epidermal growth factor receptor (EGFR) is dysregu-lated in various types of malignancy as a result of gene amplification,mutations, or abnormally increased ligand production. Therefore, ... S(2007) Effect of combining epidermal growth factorreceptor inhibitors and cisplatin on proliferation and apoptosis of oral squamous cell carcinoma cells. Int JOncol30, 146 9–1 476.55 Ciardiello ... can transmit signals for re-organization of the cytoskeleton, the formation of lamellipodia, mem-brane ruffling and changes in cell morphology.Accordingly, dysregulation of the EGFR contributesto...
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Tài liệu Báo cáo khoa học: Hepatic stimulator substance mitigates hepatic cell injury through suppression of the mitochondrial permeability transition pdf

Tài liệu Báo cáo khoa học: Hepatic stimulator substance mitigates hepatic cell injury through suppression of the mitochondrial permeability transition pdf

... augmenter of liver regeneration(ALR) [16]. In 1996, the cDNA sequence of ALRwas reported by Giorda et al. [17]. Unlike completemitogens such as hepatic growth factor or transform-ing growth factor-a, ... pretreatment with a gradient of either CsA (0.1, 1.0, 10 or 15 lm ) or its analog NIM811(0.1, 1.0, 10 or 15 l m) for 15 min, the cells were incubatedwith CCCP (30 lm) for 1 h, and the mitochondria ... besignificant.AcknowledgementThis work was supported by grant from the National‘863’ Project of the Ministry of Science and Technol-ogy China (2006AA02A410).References1 LaBrecque DR & Pesch LA (1975) Preparation and partial...
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Tài liệu Báo cáo khoa học: Pronounced adipogenesis and increased insulin sensitivity caused by overproduction of prostaglandin D2 in vivo pptx

Tài liệu Báo cáo khoa học: Pronounced adipogenesis and increased insulin sensitivity caused by overproduction of prostaglandin D2 in vivo pptx

... 2010 FEBS and size of adipocytes. A major role of adipocytes isto store large amounts of triglycerides during periods of energy excess and to mobilize these depots duringperiods of nutritional ... anti-(rabbit IgG) for 30 min (Vector Laboratories, Burlingame,CA, USA), and this was followed by staining with theavidin–biotin–peroxidase complex system (Vectastain ABCKit; Vector Laboratories). Immunohistochemical ... Rheumatology, Immunology, and Allergy, Brigham and Women’s Hospital,Boston, MA, USA3 Laboratory of Molecular Function of Food, Division of Food Science and Biotechnology, Graduate School of Agriculture,...
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