Báo cáo khoa học: Functional analysis of the methylmalonyl-CoA epimerase from Caenorhabditis elegans docx

Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt

... with BMAL1 and the bin ding to the E-box.Substitution of Arg65 for Ala abolished the interaction of DEC1 with BMAL1 and the binding of DEC1 to the E-box.However, substitution of His57 for Ala ... increase in the mutantmice, the disruption of DEC1 might be compensated byDEC2. Further investigation is required to clarify the functions of DEC1 and DEC2. The suppressive activities of DEC1 and ... deletion of 9 3 residues (DEC1:94–412) or more than 93 residues ( DEC1:119–412)abolished the suppression (Fig. 1A). On the other h and,deletion of up to 273 residues from the C-terminal regionof...

Báo cáo khoa học: Functional analysis of the aglycone-binding site of the maize b-glucosidase Zm-p60.1 pot

... introducingfeatures of the consensus motif into the Zm-p60.1scaffold, an analysis of structural and catalytic prop-erties of the mutants, and simulations of the sub-strate–enzyme interactions of the wild-type ... F200Kand P2. Furthermore, the F193A and W373K muta-tions do not result in any change in the thermostability of the enzyme (Table S2), and thermal unfolding of the wild-type and both the F193A and ... anal-ysis of plant b-glucosidases to obtain insights into the evolution of the molecular sites involved in the twomodes of aglycone binding. The amino acid sequence of Zm-p60.1 was com-pared with the...

Báo cáo khoa học: Functional analysis of the methylmalonyl-CoA epimerase from Caenorhabditis elegans docx

... in Caenorhabditis elegans. Nat Genet 17, 119–121.J. Ku¨hnl et al. Methylmalonyl-CoA epimerase from C. elegans FEBS Journal 272 (2005) 1465–1477 ª 2005 FEBS 1477 Functional analysis of the methylmalonyl-CoA ... carboxylase catalyses the formation of the S-epimer of methylmalonyl-CoA. For further catalysis by the vitamin B12-dependentKeywords Caenorhabditis elegans; epimerase; methylmalonyl-CoA CorrespondenceE. ... members of the VOCsuperfamily. Superimposing P. shermanii MCE on the human glyoxalase structure shows that the Co2+ion of the MCE is only 0.2 A˚ from the position of the Zn2+ion in the glyoxalase...

Báo cáo khoa học: Functional domains of the human epididymal protease inhibitor, eppin docx

... The bars represent the mean percentagesurvival of two samples of bacteria and the error bars the SDs of these means. Percentage survival was calculated from the fraction of treated cells surviving ... of the bacteria with the proteins, no reduction of the XTTwas observed (data not shown). This suggests that the bacteria were now dead and also that the reduction of XTT was due to enzymes of ... proteins) carried out in parallel. The SDs of the colony countsfor the control samples were no greater than 16% of their means.Table 1. Deconvolution of CD spectra of the three fragments.Spectra...

Báo cáo khoa học: Functional analysis of a murine monoclonal antibody against the repetitive region of the fibronectin-binding adhesins fibronectin-binding protein A and fibronectin-binding protein B from Staphylococcus aureus pot

... in the binding of the ligand to the other repeats that share a common epi-tope. Other inhibition mechanisms cannot be excluded;among these, there is the possibility that, through the effect of ... the effect of the proximities of the antibody-binding andFn-binding sites, the interaction of the repeat with Fnmay be sterically hindered in the presence of the anti-body. The results of this study ... C-terminal segment of the repeat. The distinct localization of the 15E11 epitope and the ﬁbronectin-binding site suggests that the interferingeffect of the antibody might result from steric hindrance...

Báo cáo khoa học: Functional expression of the quinoline 2-oxidoreductase genes (qorMSL) in Pseudomonas putida KT2440 pUF1 and in P. putida 86-1 Dqor pUF1 and analysis of the Qor proteins doc

... estimated from Hanes plots.Determination of the metal contents of the Qor proteins,and detection of the nucleotide moiety of the molybdenum cofactor The contents of molybdenum and iron were determined ... qor genes and theirﬂanking regions. The plasmid pBG3a was digested with XhoIandDraIIIto remove nptII. After the removal of the 3¢ overhang and the ﬁlling of the 5¢ overhang of the plasmid with ... of the molybdenum cofactor.Synthesis of Mo-MGD from Mo-MPT and Mg2+-GTP iscatalyzed by the MobA protein [30–36]. Possibly, E. colilacks an enzyme that catalyses the formation of Mo-MCDfrom...

Báo cáo khoa học: Functional analysis of pyrimidine biosynthesis enzymes using the anticancer drug 5-ﬂuorouracil in Caenorhabditis elegans docx

... inC. elegans to test their roles in 5-FU function andwere able to identify homologs for most of them inC. elegans. To explore the functional relationships of thesepyrimidine biosynthesis ... thatknockdown of these two genes is very speciﬁc.To more precisely understand the functions of theseuridine kinase homologs, we analyzed their enzymeactivity in vitro. The proteins puriﬁed from the ... and5-FU. Uncovering the mechanism of 5-FU resistanceand characterization of pyrimidine biosynthesisenzymes in C. elegans will help to further our under-standing of pyrimidine biosynthesis enzymes...

Tài liệu Báo cáo khoa học: Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate speciﬁcity docx

... for the chemotherapeutic treatment of cancer and viraldiseases because they catalyse the addition of the ﬁrstphosphate group to the nucleoside analogue. Thisprimes the nucleoside for further ... in hydrogenbonding. The result that E52Q did not show any activitymust therefore be a consequence of the reactivity of the functional group and further support the hypothesisthat E52 acts ... phosphorylation of the wild-type. Relativephosphorylation expressed as a relation of the percentage of dThdphosphorylation of the corresponding mutant is given in paren-theses. The substrate...

Tài liệu Báo cáo khoa học: Quantitative analysis of the experimental O–J–I–P chlorophyll ﬂuorescence induction kinetics Apparent activation energy and origin of each kinetic step Steve Boisvert, David Joly and Robert Carpentier doc

... amplitudes and rate constants of the two other phases. The above should be gov-erned by the balance between the rate of reductionand oxidation of the acceptor side of PSII by the avail-able electron ... half-time of the J–I phase were bothdecreased with DCMU, demonstrating that the J–I riseis not directly related to the reduction of the PQ pool.A further analysis of the inﬂuence of PQ reductionon ... correspond to the end of the J–I phase [8,28,29]. It is likely that the emergence of a peak for I in the FI curves depends on the relative amplitude and rate constant of the J–Iphase compared to the amplitudes...

Tài liệu Báo cáo khoa học: Functional dissection of the Schizosaccharomyces pombe Holliday junction resolvase Ydc2: in vivo role in mitochondrial DNA maintenance pptx

... centre of the junctions [19]. Detailedunderstanding of the functional role of these interactionswould require the crystal structure of the Ydc2–junctioncomplex.In this study the function of several ... Binding of CCE1 results in the complete unpairing of the four base-pairs in the centre of the junctions with a lesser disruption of the next base-pairs[19], which is reminiscent of the structure of ... using either of the truncated proteins under any of the conditions tested (datanot shown). The improved binding pattern of the double mutant,compared to Ydc2-ND35 suggested that removal of the N-terminal...

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