... 2 72, 16 01 16 07. 17 . Inohara-Ochiai, M., Okada, M., Nakayama, T., Hemmi, H.,Ueda, T., Iwashita, T., Kan, Y., Shibano, Y., Ashikari, T. &Nishino, T. (2 000) An active-site m utat ion causes ... 2002di- a nd oligo-saccharides and starch at comparable rates[23,24]. The sequence classi®es a- glucosidases in glycosidehydrolase families 13 and 31 [25±27]. Yeast a- glucosidases and oligo -1, 6-glucosidase ... compared to that of glucoamylase of glycosidehydrolase family 15 or of a type II a- glucosidase from theretaining glycoside hydrolase family 31 [9 ,10 ,40, 41] .MATERIALS AND METHODSEnzymes and...