Báo cáo khoa học: Novel isoenzyme of 2-oxoglutarate dehydrogenase is identiﬁed in brain, but not in heart potx

Tài liệu Báo cáo khoa học: Novel aspects of heat shock factors: DNA recognition, chromatin modulation and gene expression ppt

... DNA-binding domain (DBD)at the amino-terminus and the oligomerization domain in the central region of the protein [1,4]. The HSFDBD belongs to the ‘winged’ helix-turn-helix family of DNA-binding ... interactions. In HSF DBD–HSE co-crystals, the protein–protein interface consists of the helix 2 amino-terminus, the turn and the wing[15]. Unlike other winged helix-turn-helix proteins, thewing of the ... BofHSF4, the protein exhibits a reduced ability to trimerizeand is unable to bind to discontinuous gap- and step-typeHSEs, but binding to the continuous 3P-type HSE is not affected [21]. In...

Báo cáo khoa học: Novel repressor of the human FMR1 gene ) identiﬁcation ¨ of p56 human (GCC)n-binding protein as a Kruppel-like transcription factor ZF5 ppt

... pairs of primers, which amplifyoverlapping 5¢- and 3¢-parts of ZF5 cDNA, were designedusing Primer software developed by V. Prutkovsky andO. Sokur of the Institute of In uenza, Ministry of Health of ... Kuteikin KB (1994)Identiﬁcation of a GC element in the promotor of themurine L32 ribosomal protein L32 gene and the 3¢-ter-minal region of the human apolipoprotein A-I gene,capable of binding ... vector pGEXZF5 encoding recombinant fusionprotein, which consists of glutathione S-transferase(N-terminal part) and the DNA-binding domain of ZF5(C-terminal part), was kindly provided by Dr S. Yamam-oto,...

Báo cáo khoa học: Novel aspects of calmodulin target recognition and activation pptx

... comple-mentarity of binding surfaces determine the binding orien-tation of the peptide in complex with CaM.The second interesting feature is the conformation of theCaMKK binding domain peptide in complex ... structure of the CaM/C24Wcomplex [67].The binding of the C20W peptide of the Ca2+-pumpCaM-binding domain to only the C-terminal lobe of CaMdemonstrates that Ôwrapping aroundÕ a CaM bindingdomain, ... residues. This charge distribution on the molecularsurface contributes to peptide binding via electrostatic inter-actions and determines the relative binding orientation of CaM-binding domain peptides....

Báo cáo khoa học: Autocatalytic processing of procathepsin B is triggered by proenzyme activity doc

... in nonselective protein degradation inside lysosomes, and is involved in the processing of other proteins and hor-mones such as trypsinogen and thyroglobulin [1–3].Secreted cathepsin B is often ... mutations in this region, includingmutations to prolines. On the basis of these results, a model for autocata-lytic activation of cysteine cathepsins is proposed, involving propeptide dis-sociation ... University School of Medicine, CA, USACysteine cathepsins comprise a group of papain-likecysteine proteases found predominantly in lysosomes.Cathepsin B (EC 3.4.22.1) is one of the most abundantand...

Báo cáo khoa học:The principle of flux minimization and its application to estimate stationary fluxes in metabolic networks docx

... self-reproduction, gaining a maximal biomass production at agiven total flux is obviously equivalent to maintaining agiven rate of biomass production at a minimum of the totalflux. Insofar, the principle of maximal ... production is a special case of the more general principle of fluxminimization. It has to be noted, furthermore, that mini-mization of fluxes in a metabolic system is closely linked tominimization of ... of the stationary state by means of the flux-minimization method is accomplished b y expressing allfluxes through the linear combinations given in column six of Table 1 and determining the minimum...

Báo cáo khoa học: The effect of replacing the axial methionine ligand with a lysine residue in cytochrome c-550 from Paracoccus versutus assessed by X-ray crystallography and unfolding ppt

... basic f-amino group of the coordinating K100, with the H-bonded watermolecule found in the X-ray structure increasing theelectron donating power of the coordinating aminogroup which in turn ... changesbeing minimal the trapping of two main-chain confor-mations and increased B-factors at 295 K suggests thatthe presence of the coordinating Lys in some way can in uence the dynamics of the ... abolishes the interaction of wat5with the Nd2 of N52 (yeast cyt c numbering). Such aninteraction is not present in cyt c-550 due to residue 59(residue 52 in yeast) being an Ile. Interestingly,...

Báo cáo khoa học: Diverging regulation of pyruvate dehydrogenase kinase isoform gene expression in cultured human muscle cells pot

... (PDP).To date, four isoforms of PDK (PDK1–4) and twoisoforms of PDP (PDP1–2) have been identiﬁed in humans [3–5]. These isoforms display unique tissue dis-tribution [3–5] and varied kinetic and regulatory ... suggesting that the MAPKsignalling cascade is not involved in transducing theinsulin-to-PDK transcriptional signal. The ability of U0126 to inhibit insulin-stimulated phosphorylation of MAPK in ... targets of PtdIns3K include glycogen syn-thase kinase-3 (GSK3) and the mammalian target of rapamycin (mTOR). GSK3 is inactivated in responseto insulin via a PtdIns3K ⁄ protein kinase B (PKB)-dependent...

Báo cáo khoa học: Sustained activation of ERK1/2 by NGF induces microRNA-221 and 222 in PC12 cells pdf

... 4B). Wealso confirmed inhibition of protein synthesis bymonitoring expression of c-Fos protein, which is aFig. 2. Activation of ERK1 ⁄ 2 pathway is involved in miR-221 ⁄ 222induction. (A, B) PC12 ... sustained activation of ERK1 ⁄ 2byNGF, but not the transient activation of ERK,could effectively induce miR221 and 222 in PC12cells. Finally we identified the BH3-only proteinBim, which is involved ... both ERK signaling and miRNA function areinvolved in a variety of important biological responses,the significance of ERK signaling in terms of regulatingmiRNA expression is of great interest.To...

Báo cáo khoa học: The occurrence of riboﬂavin kinase and FAD synthetase ensures FAD synthesis in tobacco mitochondria and maintenance of cellular redox status docx

... photosynthesis,metabolism of fatty acids, some amino acids, cholineand betaine, and synthesis of vitamin B6, vitamin B12,folate, and protoporphyrin. FAD is also the coenzyme of glutathione ... oxidase-dependent folding of secretoryproteins also depend on FAD [3–5]. In plants, FAD is involved in ascorbate (ASC) bio-synthesis and recycling, thus playing a crucial role in cell defence against oxidative ... a maximum value of 3600 pmolÆmg)1protein within 15 min of incubation. Prolonging theincubation time resulted in a signiﬁcant decrease in the amount of FAD. With prolonged incubation, thehydrolytic...

Báo cáo khoa học: A role of monocyte chemoattractant protein-4 (MCP-4)/CCL13 from chondrocytes in rheumatoid arthritis doc

... chemokine receptors, includingCCR-2 and CCR-3 [12,13]. The role of MCP-4 ⁄CCL13 in disease is less well defined, but recent studiessuggest that it is involved in inflammatory cell recruit-ment in ... involved in the joint degrada-tion process in RA. Thus, MCP-4 ⁄ CCL13 might haveroles not only in the proliferation of synovial cells but also in the invasion of synovial cells, resulting in ... evidence of persistent synovitis or minimal clini-cal benefit [1]. The results of the present study suggestthat IFN-c may contribute to the progression of joint in ammation, in part by modulating...

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