Báo cáo khoa học: Production and characterization of a noncytotoxic deletion variant of the Aspergillus fumigatus allergen Aspf1 displaying reduced IgE binding ppt

Tài liệu Báo cáo khoa học: Production and characterization of a secreted, C-terminally processed tyrosinase from the ﬁlamentous fungus Trichoderma reesei ppt

... TCA GGG CAC GAC ACA CAT CCC C; and reverse primer, GAT CGG TAC CTC ATT ACA GAGGAG GGA TAT GGG GAA C. The PCR reaction wasdone as described above. The ampliﬁed PCR product wasinserted into the ... oxidase, Japanese patent 61115488.37 Yamada Y, Tawara Y & Yoshika H (1983) Production of heat-resistant polyphenol oxidase, Japanese patent60062980.38 Abdel-Raheem A & Shearer CA (2002) ... indicator substrate was used. The trans-formants were streaked on the plates and grown for 7 days, and tyrosinase activity was observed on the plates as a brown color appearing around the streaks. Positive...

Tài liệu Báo cáo khoa học: "Liars and Saviors in a Sentiment Annotated Corpus of Comments to Political debates" pdf

... (Ga-napathibhotla and Liu, 2008). Our annotation scheme stands on the following assumptions: (i) the sentence is the unit of analysis, whose interpretation may require the analysis of the ... states were manually annotated. The annotation was per-formed at word and phrase level, and the sentiment expressions identified in the corpus were asso-ciated to the source of the private-state, ... the of comments and the number of votes of each cadidate (Table 1). , in which the number of positive and negative sentences is relatively balanced, all the remaining debates generated...

Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx

... in the Caryophyllaceae, Sambucaceae,Cucurbitaceae, Euphorbiaceae, Phytolaccaceae and Poaceae [1]. Although many are potentially useful inagriculture and medicine because of their antiviralproperties ... RIPnamed pulchellin. It exhibits speciﬁcity for galactose and galactose-containing structures, can agglutinatehuman and rabbit erythrocytes, and kills mice and the microcrustacean Artemia salina ... displayed hemagglutination and toxicitytoward mice (data not shown), additional efforts tocleanly isolate the isoform were not successful and further characterization was abandoned. A chromato-focusing...

Tài liệu Báo cáo khoa học: Cloning and characterization of CBL-CIPK signalling components from a legume (Pisum sativum) ppt

... forward)25¢-ACAAA (A ⁄ C )A( A ⁄ G) (A ⁄ G ⁄ T )A( C ⁄ T) (A ⁄ C ⁄ G)ACACCACAAGACC)3¢ PsCIPK (degenerate reverse)35¢-CTTAT(C ⁄ G)AACAAGGAA (A ⁄ C)AATTTC-3¢ PsCBL (degenerate forward)45¢-GTATCAGCTTC(C ⁄ T)TCAAATGTC-3¢ ... (degenerate reverse)55¢-CCATCACAAGAAACTAGAGAAAC-3 PsCIPK (5¢UTR forward)65¢-TTAAGTACTATAAAT-ACACAGCCTA-3¢ PsCIPK (3¢UTR reverse)75¢-CGAGCTCACTGCCTCTCAAC-3¢ PsCBL (5¢UTR forward)85¢-ACTCGTAGC-ACAGAGACAGAG-3¢ ... 1, of EF1 is replaced by amino acid A (Fig. 1B). The EF1 and EF2 are 22 amino acids apart,whereas EF2 and EF3, and EF3 and EF4 are 25 and 32 amino acids apart, respectively (Fig. 1B). The cal-cineurin...

Tài liệu Báo cáo khóa học: Cloning and characterization of two distinct isoforms of rainbow trout heat shock factor 1 ppt

... follows: HSF 1a forward,5¢-GAAGCAGCTTGTCCAGTACACCAA-3¢; HSF 1a reverse, 5¢-TTCCAAGAGCTGAACAAACCATTG-3¢;HSF1b forward, 5¢-GAAGCAGCTGGTCCAGTACACCTC-3¢; HSF1b reverse, 5¢-GGCTGAATAAACCATGCCAGTAGC-3¢; ... Plasmid Cloningkit (Invitrogen) and was used as the PCR template. For5¢-RACE, the ﬁrst PCR was performed with the M13 reverseprimer (5¢-AGCGGATAACAATTTCACACAGG-3¢)asasense primer and a rainbow ... toextract total RNA for RT-PCR, were obtained from the Nikko Branch of the National Research Institute of Aquaculture (Tochigi, Japan) and reared on a commercialdiet at 15 °C.Cloning of HSFcDNAA...

Báo cáo khoa học: Synthesis and characterization of a new and radiolabeled high-afﬁnity substrate for H+/peptide cotransporters pdf

... Gly-Gln, Ala-Ala, Ala-Ala-Ala, Lys-Lys, d-aminolevulinic acid, cefadroxil,Gly, Pro-Ala, 8-aminooctanoic acid and Gly-Sar were fromSigma-Aldrich (Deisenhofen, Germany). Tert. butyloxycar-bonyl ... h. The amount of Bip-Pro in the extracellular uptake mediumwas quantiﬁed according to the laboratory standard HPLC(La-ChromÒ; Merck-Hitachi, Darmstadt, Germany) with a diode array detector and ... More-over, after radioactive labeling we determined the kinetic parameters and transport characteristics of Bip-[3H]Pro.Results and DiscussionSynthesis, chemical characterization and stabilityof...

Báo cáo khoa học: Engineering and characterization of human manganese superoxide dismutase mutants with high activity and low product inhibition potx

... wereplated again on 1 lm paraquat agar plates, so as toobtain approximately 500 colonies per plate. Afterincubation of these replated mutant colonies at 37 °Cfor another 20–24 h, the average ... However, the backbone amide and carbonyl of Cys140 form hydro-gen bonds with the backbone carbonyl and amide of Trp123, the side chain of which forms one wall of the active site cavity and the replacement ... action of mutant C140S–Q14 3A hMnSOD relative to the other variants of hMnSOD containing the mutationsQ14 3A, C140S and N73S (Table 2 and Fig. 1) is notimmediately clear. The data appear consistent...

Báo cáo khoa học: Cloning and characterization of the genes encoding toxic lectins in mistletoe (Viscum album L) pot

... and XhoIML3p A- chain5¢-GATATACATATGTACCGTCGTATTAGCCTTCGTGTCACGGAT-3¢5¢-CACACGAATTCTTATTAAGAAGAAGAAGAACGGTCCCTGCATAC-3¢NdeI and EcoRIML3.1p A- chain5¢-GATATACATATGTACGAGCGTCTTCGTCTTCGTGTTACGCATC-3¢5¢-CACACGAATTCTTATTAAGAAGAAGAAGAACGGTCCCTGCATAC-3¢NdeI ... and XhoIML2p A- chain5¢-GATATACATATGTACGAGCGTCTTCGTCTTCGTGTTACGCATC-3¢5¢-CACACCTCGAGTTATTAAGAAGAAGACGGACGGTCCCGGCATAC-3¢NdeI and XhoIML3p A- chain5¢-GATATACATATGTACCGTCGTATTAGCCTTCGTGTCACGGAT-3¢5¢-CACACGAATTCTTATTAAGAAGAAGAAGAACGGTCCCTGCATAC-3¢NdeI ... amplify the full-length coding sequences a set of primerswas derived from 3¢ and 5¢ untranslated regions (UTR)obtained by RACE. The sequences of the primers were:5¢AAAATCTAGAGAAGCAAGGAACAATGAATG-3¢(5¢UTR)...

Báo cáo khoa học: Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL family docx

... 5¢-gcgccaatcctggacgctgacgtcatcgacgcg-3¢ (forward) and 5¢-cgcgtcgatgacgtcagcgtccaggattggcgc-3¢ (reverse). The bases in bold indicate the location of the mutation. DNAsequence of the mutant was conﬁrmed ... strands associated to an anti-parallel strand (b2) and is surrounded by 5 helices (a1 , a2 , a3 , a7 anda8). T he second domain c onsists of helices a4 , a5 and a6 a ll clustered on the top of t he ... His290 and Asp260 e mergefrom the b8 -a8 andb7 -a7 loops, respectively. Anotherimportant feature of the catalytic machinery is the so-calledoxyanion hole necessary for the stabilization of the oxyan-ion...

Báo cáo khoa học: Discovery and characterization of a Coenzyme A disulﬁde reductase from Pyrococcus horikoshii Implications for the disulﬁde metabolism of anaerobic hyperthermophiles doc

... act-ing as a CoADR. This is only the second demonstra-ted CoA reductase activity, and the first appearance of this activity in both the Archaea and in a strict anaer-obe. While the best known small ... concentration(as determined at 460 nm). blast and tfasta analysis of the phCoADR revealed a significant level of identityto putative NADH oxidases from hyperthermophiles and bacterial NADH oxidases ... form), a significant amount of oxidase activity can beobserved in the presence of additional substrate-levelFAD (Table 2). The kcat appobtained in the presence of 100 lm NADH and FAD and 115...

Xem thêm