... occur during e xposure to pressure. The firstparameter to change D2, shows a clear d ependence uponprotein concentration, indicating that D2 is monitoringdissociation of t he protein into monomers. ... hydrophobicthan the interior. In addition, several polar amino acids,especially arginine, lysine, glutamine and glutamate arefound more frequently at the interface than in the interior.The degree ... on protein concentration, indicating that eno-lase is dissociating into monomers. The small changes in theUV spectrum a nd the retention of activity lead to a model in which enolase, in t he...