... The Authors Journal compilation ª 2008 FEBS KCTD5, a putative substrate adaptor for cullin3 ubiquitin ligases Yolanda Bayo´n1, Antonio G. Trinidad1, Marı a L. de la Puerta1, Marı a del ... 12CA5 mAb against HA was from Roche(Indianapolis, IN, USA), anti-HA clone HA.11 was fromCovance (Berkely, CA, USA), anti-glutathione S-transferase(GST) and mAb against myc (9E10) were from Santa ... to cullin3. These findings suggest that KCTD5 is a substrate- specific adaptor for cullin3- based E3 ligases. AbbreviationsAU, arbitrary unit; BTB, bric -a- brac, tramtrak and broad complex; CT, cycle threshold;...