Báo cáo khoa học: Insights into substrate and product trafﬁc in the Drosophila melanogaster acetylcholinesterase active site gorge by enlarging a back channel ppt

... Insights into substrate and product traffic in the Drosophila melanogaster acetylcholinesterase active site gorge by enlarging a back channel Florian Nachon1, Jure Stojan2 and Didier ... hydrolysisbecause the traffic can pass via an alternative route, presumably the enlarged back channel. AbbreviationsCAS, acylation site; DmAChE, Drosophila acetylcholinesterase; PAS, peripheral site. FEBS ... used by at most 5% of the traffic, with the majority of traffic pass-ing through the main entrance. In mutants Trp83Ala and Trp83Glu,ligands that close the main entrance do not inhibit substrate...

Tài liệu Báo cáo khoa học: Processing, catalytic activity and crystal structures of kumamolisin-As with an engineered active site pptx

... enhance-ment of the 57-kDa band with a concomitant diminu-tion of the 38-kDa and 19-kDa bands, as analyzed by SDS ⁄ PAGE (Fig. 3A) . The 38-kDa and 19-kDa bandsdid not disappear completely after ... proteinase. Structure10, 865–876.7 Wlodawer A, Li M, Gustchina A, Tsuruoka N, AshidaM, Minakata H, Oyama H, Oda K, Nishino T &Nakayama T (2004) Crystallographic and biochemicalinvestigations ... mediates the interactions betweenSer278 and Asp164. Thus the introduction of an aspa-ragine instead of an aspartic acid into the oxyanionhole had the unexpected result of shifting the sidechain...

Tài liệu Báo cáo khoa học: Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate speciﬁcity by replacement of Asn149 with Asp docx

... agmatinase activity of the Asn149Asp vari-ant, it is clear that the interactions of arginase II withl-arginine and agmatine are greatly altered by replace-ment of this residue with aspartate. ... 5¢-CTGGGAGGAGACAACAGCCTGGCAATC-3¢ for His120Asn, 5¢-TGGGTTGATGCCAATGCTGACATCAAC-3¢ for His145Asn and 5¢-TGACATCGACACACCCC-3¢ for Asn149Asp.Fluorescence spectra and thermal inactivationstudiesFluorescence ... fully active state. In contrast, the arginaseactivity of the Asn149Asp variant was practicallyundetectable, both before and after the incubation with the manganese ions.Fully active His120Asn and...

Tài liệu Báo cáo khoa học: Insights into and speculations about snake venom metalloproteinase (SVMP) synthesis, folding and disulﬁde bond formation and their contribution to venom complexity pdf

... N-terminal to the unpaired cysteinyl residues observed in jararhagin and catrocollastatin. Jarahagin and catrocollastatin areknown to undergo post-translational processing toyield a disintegrin-like ... pairs located in the center and in the C-terminalregion of the domains are shared between the disinte-grin ﬂavoridin and VAP1. The disulﬁde bond patternsof the disintegrins and disintegrin-like ... bothfull-length jararhagin and catrocollastatin and theirprocessed disintegrin-like ⁄ cysteine-rich domains(jararhagin C and catrocollastatin C) products couldbe isolated from their respective...

Tài liệu Báo cáo khoa học: Insights into the structure of plant a-type phospholipase D Susanne Stumpe, Stephan Konig and Renate Ulbrich-Hofmann ¨ ppt

... membersof the PLD superfamily. However, there is low similar-ity in the remaining parts of the molecules. Most mam-malian PLDs contain a PX and a PH domain insteadof the C2 domain in plants, whereas ... latter being dominant. The ﬂuorescence and near-UV CD spectra point to tight packing of the aromatic residues in the core of the protein. From the near-UV CD signals and activity data as a function ... PLD a2 from cabbageare given. The surface structure of the enzyme wasinvestigated by small-angle X-ray scattering (SAXS).Further structural information was obtained by the spectroscopic characterization...

Tài liệu Báo cáo khoa học: "Insights into Non-projectivity in Hindi" docx

... put before the publisher and talked [to him]_ROOT_ us__lekhakiiy__asmitaa__ko ham sagarv prakaashak__ke−saamane rakhakar baat__karate_ _the b) a) _ROOT_ isake__baad vah jamaan__shaah aur−phir 1795__meM ... iden-tifying appropriate karakas. The dependency tagset in the annotationscheme has 28 relations in it. These includesix basic karaka relations (adhikarana [location],apaadaan [source], sampradaan ... can easily bemade into a projective construction, which is also the more natural construction for it.ham us lekhakiiy asmitaa kosagarv prakaashak ke-saamanerakhakar baat karate the 5.9 OthersThere...

Tài liệu Báo cáo khoa học: Staphylococcal enterotoxin C1-induced pyrogenic cytokine production in human peripheral blood mononuclear cells is mediated by NADPH oxidase and nuclear factor-kappa B doc

... obtain totalchemiluminescence.Statistical analysis The animals were maintained at an ambient temperature of22 °C for at least 90 min to obtain the thermal balancebefore any tested agent was ... Colavita AR, FitzGerald GA & Violi F(1997) Platelet activation by superoxide anion and hydroxyl radicals intrinsically generated by platelets thathad undergone anoxia and then reoxygenated. ... Ohta M, Kamata S & Kumagai K (2003) Concentra-tions and speciﬁc antibodies to staphylococcal entero-toxin-C and toxic shock syndrome toxin-1 in bovinemammary gland secretions, and in ammatory...

Báo cáo khoa học: Coordination of three and four Cu(I) to the a- and b-domain of vertebrate Zn-metallothionein-1, respectively, induces signiﬁcant structural changes doc

... Boﬁll R, Palacios O, Capdevila M, Cols N, Gonzalez-Duarte R, Atrian S & Gonzalez-Duarte P (1999) A newinsight into the Ag+ and Cu+ binding sites in the met-allothionein beta domain. J Inorg ... using the program dyana and the published solution structure of the respective cad-mium-containing domain of the intact protein as a starting point. The resulting structure family had a tar-get ... higher than ﬁve.Taken together, the initial additions of Cu(I) to eachdomain caused the disappearance of a large set ofNOESY cross-peaks and the parallel appearance ofanother set of cross-peaks,...

Báo cáo khoa học: Crosstalk between Src and major vault protein in epidermal growth factor-dependent cell signalling docx

... tyrosine kinaseactivity. Domains within Src kinases target the enzyme to speciﬁc subcellular locations where theybind to regulatory and ⁄ or substrate proteins and areintegrated into cell signalling ... withphosphorylated Tyr527 in the C-terminal tail bindingto the SH2 domain. The ligand binding surfaces of the SH2 and SH3 domains are tucked inside, thus present-ing an inert surface to the outside environment ... tyrosine kinase. The experiment was performed using the same Src kinase assay as in (A) , without enolase. The resultindicates that MVP is a substrate of Src tyrosine kinase in vitro aswell.α...

$Tài liệu Báo cáo khoa học: Identiﬁcation of different isoforms of eEF1A in the nuclear fraction of human T-lymphoblastic cancer cell line speciﬁcally binding to aptameric cytotoxic GT oligomers ppt$

Tài liệu Báo cáo khoa học: Identiﬁcation of different isoforms of eEF1A in the nuclear fraction of human T-lymphoblastic cancer cell line speciﬁcally binding to aptameric cytotoxic GT oligomers ppt

... The gel was then dried and auto-radiographed. In the UV cross-linking assay, the samples were incuba-ted at room temperature, as described above for the EMSAassay, and then irradiated at 302 ... morphogenesis, a sixfold elevation in eEF 1A speciﬁc activity is accompanied by a dramaticincrease in protein methylation at as many as nine lysineresidues, without any change in the protein or mRNA levels[43]. ... and incubated with mAb for eEF 1A, as described in the Materials and methods. Lane 1, bacterial recombinant eEF 1A protein(R eEF 1A) ; lane 2, eEF 1A protein from total nuclear extracts (NEeEF 1A) ;...

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