Báo cáo khoa học: The N-terminal hybrid binding domain of RNase HI from Thermotoga maritima is important for substrate binding and Mg2+-dependent activity pot

... showsprocessivity for long RNA ⁄ DNA substrates and losesthis processivity on removal of the HBD. Binding to substrate To examine whether the HBD of Tma -RNase HI is important for substrate binding, the binding ... catalytic domain (residues 64–223) of Tma -RNase HI; Tma-ND, N-terminal domain (residues 1–63) of RNase HI from Thermotoga maritima containing HBD; Tk -RNase HII, Thermococcus kodakaraensis RNase HII.4474 ... human RNase H1 (Hsa -RNase H1), E. coli RNase HI (Eco -RNase HI) and the RNase H domain of HIV-1 reverse transcriptase (HIV-1 RNase H) in Fig. 1. The HBD of Tma -RNase HI shows relatively high amino...

Báo cáo khoa học: The Drosophila jumonji gene encodes a JmjC-containing nuclear protein that is required for metamorphosis pot

... inter-action domain of dJmj for these factors is the N-termi-nal repression domain, which is evolutionarilyconserved among Jmj proteins (Fig. 1). Detailed analy-sis of the role of N-terminal and the ... structures of mouseJmj, Drosophila Jmj and Lid. The locations of the JmjN domain, JmjC domain, ARID,PHD, AT-hook domain and C5HC2 zinc ﬁn-ger domain are shown. (B) Amino acid align-ment of the N-terminal ... Machida, Japan The basic unit of chromatin in eukaryotes is the nucleosome, which consists of 146 bp of DNAwrapped around an octamer of histones H2A, H2B,H3 and H4 [1]. Covalent modiﬁcations of...

Báo cáo khoa học: The natural mutation by deletion of Lys9 in the thrombin A-chain affects the pKa value of catalytic residues, the overall enzyme’s stability and conformational transitions linked to Na+ binding pdf

... single band of ~ 36 kDa for all the thrombin forms.Effect of pH on thrombin amidase activity and binding of the inhibitor a-NAPAP to the thrombinactive site The effects of pH (5.5–10) on the hydrolysis ... flexibility of the environment of Trp207 and 29 may significantly affect the intrinsic fluorescence of the DK9 variant, the higher the surface exposure and flexibility of their side-chainsto the solvent the ... [1,2]. Measure-ments of the pH dependence of both steady-state ami-dase activity and binding of the high-affinity inhibitora-NAPAP showed pKavalues of the catalytic His57higher in DK9 mutant...

Báo cáo khoa học: The variable C-terminal extension of G-protein-coupled receptor kinase 6 constitutes an accessorial autoregulatory domain ppt

... Analysis of the genomic organ-ization of the mouse GRK6 gene showed that the fourmRNAs are generated by alternative RNA splicing from a single gene. Three of the GRK6 isoforms,GRK6-A, -B and ... Three isoforms, mGRK6-A to mGRK6-C differ in their C-ter-minal-most portion, which is known to mediate membrane and ⁄ or receptorinteraction and regulate the activity of GRK4-like kinases. One isoform,mGRK6-D, ... 244 within the predicted catalytic domain (dashed vertical line). The positions of the synthetic peptides used to generate antisera PV1, sc-566 and PV2 are illustrated. The numbering of the amino...

Báo cáo khoa học: Studies on structure–function relationships of indolepyruvate decarboxylase from Enterobacter cloacae, a key enzyme of the indole acetic acid pathway ppt

... analysis of substrate speciﬁcity and cofactor binding of EcIPDC. The enzymatic conversion of all substrates studied in this work (pyruvate, the native substrate of PDC, benzoylformate, the native substrate ... addition of the cofactors. The enzyme is destabilized at low ionicstrength. The stability of EcIPDC in aqueous solutions is higher than that of other PDCs. The rate constant of inactivation of PDC from ... substitutions of this substrate at the 4-position increase the afﬁnity for the enzyme and decrease the turnover rate considerably(Table 1). The integrated Michaelis–Menten equation wasused for the determination...

Báo cáo khoa học: The proximity between C-termini of dimeric vacuolar H+-pyrophosphatase determined using atomic force microscopy and a gold nanoparticle technique ppt

... translocation of protons from the cyto-sol to the vacuolar lumen, at the expense of PPihydrolysis, for the storage of ions, sugars, and other metabolites. Dimerization of V-PPase is neces-sary for ... surface. The volume of the V-PPase homodimers (Vm) in the reconstituted membrane was estimated using the height of the protein protrusion and the thickness of the lipidbilayer as the parameters for ... 7B),indicating the dimeric structure of the enzyme. The his-togram showing the distribution of distances betweenGNP pairs observed from the TEM image yields a meandistance of 1.9 ± 0.8 nm...

Báo cáo khoa học: The N-terminal cysteine pair of yeast sulfhydryl oxidase Erv1p is essential for in vivo activity and interacts with the primary redox centre doc

... shown for the Erv2 protein, the dimer formationdepends on an interaction of the CGC motif with the CXXC motif of the opposite monomer. The short form of the Erv1 protein, which lacks the N-terminal ... serine destroys the disulﬁde bridge and the introduction of a polar group probably interfereswith binding of the adenine portion of the FAD that is normally attached to this region of the protein. ... corresponding cysteines of Erv1p. The reason for this exact match is clear from the structure of Erv2p, because the disulﬁde bridge between these residueshas to tether the short helix 5 to the four-helix-bundle...

Báo cáo khoa học: The allene oxide cyclase family of Arabidopsis thaliana – localization and cyclization ppt

... aninhibitor of AOC (see below), is essential for binding to AOC from corn [18] is consistent with the observed binding position in corn AOC [23]. Any shift of the epoxy group would inhibit the formation ... nature of the side chains of Asn and Leu and steric con-straints imposed by the two methyl groups of Leu on the substrate. The ﬁnding that the location of the epoxy group in the 12,13-position of ... by the mechanism of anchimeric assistance. The formedoxyanion is stabilized by a water molecule (water75),which is tightly bound and positioned by the forma-tion of hydrogen bonds with the...

Báo cáo khoa học: The multifarious short-term regulation of ammonium assimilation of Escherichia coli: dissection using an in silico replica pdf

... of ammonium. This partial robustness of the net ammonium assimilation ﬂux is achieved by the active regulation of the system, which is dominated by the adenylylation of GS. The constancy of the ... prediction is based upon the comparison of the calculated behaviour of our in silicoATase, PII and UTase mutants. The removal of eitherone of the three proteins at the time of an upshift in the ammonium ... pulse.Analysis of regulation of the transient response of the GS rate The decrease in the rate of GS upon the sudden addition of ammonium at time zero (Fig. 3A,B) is a result of the regulatory...

Báo cáo khoa học: The )148 to )124 region of c-jun interacts with a positive regulatory factor in rat liver and enhances transcription Dipali Sharma*, Sujata Ohri and Aparna Dixit ppt

... amounts of divalent cations are necessary for the formation of complexbetween RLjunRP and the )148 to )124 region of c-jun, and so the optimum amount of MgCl2required for complexformation was then ... India is duly acknowledged for the Senior Research Fellowshipsto D.S. and S.O. The technical assistance of S. Singh is sincerelyappreciated. The animal work included in this paper had the approval of ... speci-ﬁcity of protein–oligo interaction (lane 2) as formamide is known to dissociate the protein factors from the recognitionsequence. The presence of high afﬁnity of RLjunRP for its cognatesequence...

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