Tài liệu Báo cáo khoa học: Role of hydroxyl group and R/S conﬁguration of isostere in binding properties of HIV-1 protease inhibitors docx

Tài liệu Báo cáo khoa học: Role of Kupffer cells in pathogenesis of sepsis-induced drug metabolizing dysfunction pptx

... shown that interactions betweenKCs and endotoxin comprise the initiating event lead-ing to hepatotoxicity in liver injury, including endotox-emia and ischemia ⁄ reperfusion injury [14]. In ourstudies, ... con-centrations of tumor necrosis factor-a and interleukin-6, and increasedhepatic mRNA levels of tumor necrosis factor-a and interleukin-6 weredecreased by depletion of KCs. Our ﬁndings suggest ... KCs,resulting in the release of proinﬂammatory mediators and the downregulation of CYP enzymes in hepato-cytes.HMGB1, a DNA -binding nuclear protein, isreleased actively by monocytes ⁄ macrophages and...

Tài liệu Báo cáo khoa học: Role of the cag-pathogenicity island encoded type IV secretion system in Helicobacter pylori pathogenesis pptx

... have indi-cated that the CagAPY-induced cell elongation pheno-type also involves tyrosine dephosphorylation of cortactin, vinculin and ezrin, which are three well-known actin -binding proteins ... phosphatasesinvolved in this scenario, however, remain unknown and do not require Shp2. Instead, CagAPYcan inhibitSrc activity both by direct interaction of both proteinsor by binding of CagAPYto ... the amount of intracellu-lar CagAPY. Cortactin, ezrin and vinculin are all Srcsubstrates, and Src inactivation causes dephosphoryla-tion of these proteins and is crucial for triggering cellelongation...

Tài liệu Báo cáo khoa học: Role of ceramide kinase in peroxisome proliferatoractivated receptor beta-induced cell survival of mouse keratinocytes ppt

... betweenCerK and PPARb, and its role in regulating keratino-cyte survival. We report that in a mouse keratinocytecell line upregulation of CERK expression by activa-tion of PPARb results in a decrease ... survival resultingfrom activation of PPARb.Activation of PPARb by LD induces CERK mRNAexpression and increases CerK activity in SP1cellsTo investigate the role of CerK and its regulation of Cer ... described in Materials and methods. PCRproducts of ChIP DNA and Input DNA were analyzed with 1% agarose gel electrophoresis (B). The bands of PCR products corresponding to the binding of PPARb...

Tài liệu Báo cáo khoa học: Role of receptor-mediated endocytosis, endosomal acidiﬁcation and cathepsin D in cholera toxin cytotoxicity pdf

... pump and in vitro vacuolar acidiﬁcation in hepatoma HepG2 cells. The vacuolar H+-ATPase inhibitor baﬁlomycin and the cathepsin D inhibitor pepstatin Apartially inhibited, both in vivo and in ... preparedfrom CT and CT-B-injected rats (Fig. 3A). An increase in ARF content was observed in endosomal fractionsisolated 5 min postinjection of CT-B, and this increasewas maintained for up to 60 min (Fig. ... addressthe in vivo compartmentalization and cytotoxic action of cholera toxin in rat liver parenchyma. Following administration of a saturating dose of cholera toxin to rats, rapid endocytosis of both...

Tài liệu Báo cáo khoa học: Role of transcription factor activator protein 1 (AP1) in epidermal growth factor-mediated protection against apoptosis induced by a DNA-damaging agent doc

... AP1 DNA binding activity increasedwithin 1 h following EGF treatment and peaked at 3 hfollowing the treatment (Fig. 5A). We also examinedthe effect of PD98059 on this binding activity and, asexpected, ... transcription, were increased in response toEGF. Pretreatment of the cells with PD98059, an inhibitor of MAP kinasekinase, inhibited the EGF-induced c-Fos and c-Jun expression, AP1 DNA binding, Bcl-XLexpression, ... 1–122),but retains the DNA binding and leucine-zipper region of c-Jun, it should function as a dominant-negativemutant of c-Jun to block wild-type c-Jun binding tothe AP1 site [32]. As shown in Fig....

Tài liệu Báo cáo khoa học: Role of K22 and R120 in the covalent binding of the antibiotic fosfomycin and the substrate-induced conformational change in UDP-N-acetylglucosamine enol pyruvyl transferase docx

... chain of K22 participates in fosfomycin binding, thus providing a rationale for the loss of fosfomycin binding to the K22V and K22E mutant proteins.However, two other positively charged amino ... charged amino acid sidechains, R397 and R120, are also involved in fosfomycin binding, and in view of the multitude of interactions, it wasassumed that deletion of a single interaction would not ... enthalpies of binding and stoichiometries weredetermined from the binding isotherm by ﬁtting a 1 : 1 binding model to the data using the software provided bythe manufacturer.ResultsReaction of fosfomycin...

Tài liệu Báo cáo khoa học: Role of hydroxyl group and R/S conﬁguration of isostere in binding properties of HIV-1 protease inhibitors docx

... 3525–3601.Ó FEBS 2004 HIV-1 Protease Inhibitors (Eur. J. Biochem. 271) 4461 Role of hydroxyl group and R/S conﬁguration of isostere in binding properties of HIV-1 protease inhibitors Hana Petrokova´1, ... onconformation of inhibitor main chain. Thus, different inhibitors differ not only in t he binding afﬁnity, bu t a lso in the degree o f freedom of the inhibitor inside the binding tunnel. Some s pecial inhibitors ... of inhibitors OE, RE and SE towards their HIV-1 proteases. Stated numbers are distancesbetween inhibitor and protease atoms in A˚.Reside of Atoms of Inhibitor complexInhibitor Protease Inhibitor...

Tài liệu Báo cáo khoa học: Local stability identiﬁcation and the role of a key aromatic amino acid residue in staphylococcal nuclease refolding pdf

... chain intoN-terminal and C-terminal centers during refolding[21]. Since then, many scientists have been interested in demonstrating this concept by using N-terminal and C-terminal fragments of ... located in the C-terminal loop of SNase playsan important role in protein stability and conforma-tional integrity. During protein unfolding ⁄ refolding,the addition of 2.5 protons (both E75 and ... Global segment interactions and the folding proﬁles in wild-type SNase. (A) W140, in loop 1 interacts with loop 2 and loop 3which forms a ‘lower neck’ network area in maintaining protein ter-tiary...

Tài liệu Báo cáo khoa học: Role of cleavage and shedding in human thyrotropin receptor function and trafﬁcking pdf

... periods of times (0–60 min)at 37 °C. The concentration of the biotinylated Ig remainingon the cell surface was then quantiﬁed by measuring the binding of [125I]Streptavidin. In the absence of ... measuring the binding of [125I]Streptavidin [30]. No Ig binding wasobserved in cells transfected with the vector alone (Fig. 4B). As shown in Fig. 4B, quantiﬁcation of cell surfaceexpression of ... receptor-biotinylated Ig complexes, revealed by[125I]Streptavidin: (cAMP in receptor-transfected cells –cAMP in control pSG5-transfected cells)/ (binding of receptor-transfected cells – binding of the...

Tài liệu Báo cáo khoa học: Role for nectin-1 in herpes simplex virus 1 entry and spread in human retinal pigment epithelial cells docx

... occur during the initial 6 h window of infection.We infected RPE cells with K26GFP [27], and stainedcells for F-actin, using phalloidin at 30 min and 6 hpostinfection, and examined the cells ... fullyunderstand HSV-1 infection of all target cells, includ-ing RPE cells, our study provides new starting pointsfor understanding viral pathogenesis in the retina and advancing novel therapies ... play a role in viralspread by mediating cell-to-cell fusion [32,33], we alsodecided to examine the role of nectin-1 in the fusion of RPE cells with viral glycoprotein-expressing cells. In asemiquantitative,...

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