Tài liệu Báo cáo khoa học: Relationships between structure, function and stability for pyridoxal 5¢-phosphate-dependent starch phosphorylase from Corynebacterium callunaeas revealed by reversible cofactor dissociation studies doc

... 3325 Relationships between structure, function and stability for pyridoxal 5¢-phosphate-dependent starch phosphorylase from Corynebacterium callunaeas revealed by reversible cofactor dissociation studies Richard ... oncatalytic activity and stability. We chose starch phosphorylase from Corynebacterium callunae (CcStP), which has been characterized biochemi-cally and structurally [ 15,16], for particular reason. ... athigh and low p H. Optimum catalytic rates for phos-phorolysis were found at around pH 7.0 for both thenative enzyme and PL -phosphorylase. In the low pHregion the pH proﬁle of kcat for PL-phosphorylase...

Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx

... MAP2 cDNA and its derivativesgenerated by PCR were cloned into pGEX-4T-2. The KINDdomains of Spir-2 and PTPN13 were generated by RT-PCR and inserted into pEGFP-C1 and pGEX-4T-2. The N- and C-terminal ... Sigma-Aldrich) and HA (catalognumber: 1867423; Roche Diagnostics, Basel, Switzerland)were used at dilutions of 1 : 1000 for immunoblotting,1 : 200 for immunocytochemistry and 1 lgÆmL)1 for co-immunoprecipitation.Primary ... neurodegen-erative and psychiatric disorders [16,17].Materials and methodsAnimalsMice (ICR) were purchased from Nihon SLC (Hamamatsu,Japan) and used in accordance with protocols approved by the Animal...

Tài liệu Báo cáo khoa học: The crystal structure of human a-amino-b-carboxymuconatee-semialdehyde decarboxylase in complex with 1,3-dihydroxyacetonephosphate suggests a regulatory link between NAD synthesis and glycolysis ppt

... residues and solvent molecules engaged inligand recognition and stabilization are drawn as balls -and- sticks and spheres, respectively. The major interactions established between the DHAP inhibitor and ... cross-rotation and translation functions in the 10–4 A˚resolutionrange. The solution of the rotation function was usedto perform the translation function calculations inboth the P3221 and P3121 ... 1,3-dihydroxyacetonephosphate(DHAP) was the most potent and was thereforeselected for our structural investigation. Our resultsprovide the ﬁrst structural image of an ACMSD in aligand-bound form, and may be used to assist...

Tài liệu Báo cáo khoa học: Competition between innate multidrug resistance and intracellular binding of rhodamine dyes pdf

... isinhibited by ATP depletion; by vanadate, which hasbeen shown to inhibit Pgp, MRP1 and MRP2 [32–34]; by MK571, which has been shown to inhibit MRP1,MRP2, MRP4 and MRP7 [26,27,35,36]; and by indo-methacin, ... was inhibited by 4-chloro-7-nitro-2,1,3-benzoxadiazole (NBD-Cl) and MK571 and, to a lesser extent, by ATP depletion, indomethacin, probenecid and vanadate. All the inhibitors,apart from NBD-Cl, ... [43],were kindly provided by P. Borst (Netherlands CancerInstitute, Amsterdam, the Netherlands) and grown inRPMI-1640 (Sigma-Aldrich, Rehovot, Israel). The CIR[44], CCRF-CEM [45] and the Jurkat [46]...

Tài liệu Báo cáo khoa học: Interactions between M proteins of Streptococcus pyogenes and glycosaminoglycans promote bacterial adhesion to host cells pdf

... required: for BM27.6, erythromycin(1 lgÆmL)1); for MC25 and 90-226emm1::km, kanamycin(150 lgÆmL)1); for BMJ71, tetracycline (5 lgÆmL)1); for JRS4 and JRS145, streptomycin (100 lgÆmL)1)andforJRS145/pLZM6 ... purified from growth media ofAP1 bacteria as described [32]. Polyclonal human IgG,albumin and fibrinogen were purchased from Sigma.Chondroitinase ABC (EC 4.2.2.4) was purchased from ICN and heparan ... mUÆmL)1) and HS lyase (1.2 mUÆmL)1)inMEMfor1 h. Additional enzyme was added to a final concentrationof 200 mUÆmL)1 and 4.8 mUÆmL)1, respectively, and incubation was continued for another...

Tài liệu Báo cáo khoa học: How to remain nonfolded and pliable: the linkers in modular a-amylases as a case study ppt

... bondsformed by hydroxyl groups (O–HÆÆÆO,  21 kJÆmol)1)are twice as strong as those formed by the amide group[21]. Furthermore, hydrogen bonds formed by the sin-gle hydroxyl donor in Ser and ... should form a rather rigid and hydrophilic peptide. The abundance of Ser and Thr residues in linkers from animals also providesnumerous potential targets for O-linked glycosylation. By contrast, ... were performed, lasting 15 and 11 ns,respectively.AcknowledgementsThis work was supported by grants from the FRS-FNRS (Fonds National de la Recherche Scientiﬁque,Belgium) to G.F. and from the...

Tài liệu Báo cáo khoa học: Proteomic analysis of dopamine and a-synuclein interplay in a cellular model of Parkinson’s disease pathogenesis docx

... overexpression, parkin and PTEN-induced putativekinase 1 loss-of -function and UCHL1 mutation, leadto an impairment of neuronal dopamine homeostasis by interfering with the vesicular storage and releasemechanisms. ... with GO classi-ﬁcations was obtained from ppi spider starting from the proteins grouped as above (Tables S2 and S3).In both cases, bioinformatic analysis revealed thatthe NF-jB pathway could ... for each sample and data wereprocessed using CellQuest software (Becton-Dickinson).Fluorescent emission of propidium iodide and AnnexinV-FITC were collected through a 575 and a 530 ⁄ 30 band-pass...

Tài liệu Báo cáo khoa học: Prion protein library of recombinant constructs for structural biology docx

... have been pre-pared for studies on the structure and function ofPrPC, and which are now available upon request for use elsewhere. The data are collected in Tables 1 and 2, and at the end of ... for 51 prion protein constructs from bank vole, cat,cattle, chicken, dog, elk, ferret, frog, fugu, horse, human, pig, sheep, turtle, and wallaby, and for 113 mouse prion protein constructs and ... et al. [26] for the numeration used in this article). This C-terminalfragment forms a globular domain both as part of thefull-length sequence and in the isolated form, and there-fore constructs...

Tài liệu Báo cáo khoa học: Brain angiogenesis in developmental and pathological processes: regulation, molecular and cellular communication at the neurovascular interface pdf

... known as functional hyperemia. Cerebralblood flow rate and vessel diameter are not fixed in spe-cific regions, but depend on the local demand for oxygen and nutrients by synaptic transmission and neuronal ... and ions is accom-panied by the movement of water, which is buffered by astrocytes. The increased Na+concentration caused by glutamate transport, and increased intracellular Ca2+levels from ... distant from blood vessels, with theexception of neural stem cells and perivascular nerves.As we previously discussed, neurons and vessels arefunctionally coordinated by sharing factors for neuro-genesis...

Tài liệu Báo cáo khoa học: Brain angiogenesis in developmental and pathological processes: therapeutic aspects of vascular endothelial growth factor doc

... in both embryogenesis and adulthood, and functions by binding with two tyrosine kinase recep-tors, VEGFR-1 (Flt-1) and VEGFR-2 (KDR ⁄ Flk-1),Fig. 1. The VEGF–VEGFR system and theinhibitors of ... by Grant-in-Aid Special Pro-ject Research on Cancer-Bioscience 17014020 from theMinistry of Education, Culture, Sports, Science and Technology of Japan, a grant from the program‘Research for ... mechanismsof lymphangiogenesis in health and disease. Cancer Cell1, 219–227.14 Heiss WD, Sobesky J & Hesselmann V (2004) Identify-ing thresholds for penumbra and irreversible tissuedamage. Stroke...

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