Tài liệu Báo cáo khoa học: Transient DNA ⁄ RNA-protein interactions docx

... REVIEW ARTICLE Transient DNA ⁄ RNA-protein interactions Francisco J. Blanco1,2and Guillermo Montoya31 Structural Biology ... recognition of DNA and RNAmolecules by proteins may produce large and relatively stable assemblies(such as the ribosome) or transient complexes (such as DNA clamps slidingthrough the DNA) . These transient ... different colors, and the DNA as an orange rod (backbone) and blue–green sticks (bases).The ﬁgure was prepared withPYMOL (http://www.pymol.org). Transient protein DNA ⁄ RNA interactions F. J. Blanco...

8
586
0

Tài liệu Báo cáo khoa học: Transient RNA–protein interactions in RNA folding docx

... thoseproteins that undergo transient interactions with RNAmolecules during their folding process or during theirassembly into RNP complexes.Static versus transient interactions RNA folding reactions ... nature of transient interactions, they are compared with static interactions, as theyhave an exactly opposite character. Tight complexes havelong lifetimes (seconds or longer), whereas RNA-protein complexes ... and in the assembly andfunctioning of RNP machines, mostly through transient interactions with the RNA. Transient RNA–protein interactions in RNA folding M. Doetsch et al.1636 FEBS Journal 278...

9
600
1

Tài liệu Báo cáo khoa học: Transient silencing of Plasmodium falciparum bifunctional glucose-6-phosphate dehydrogenase) 6-phosphogluconolactonase docx

... Table 4. The qRT-PCR involved 1 cycle eachof 50 °C ⁄ 2 min and 95 °C ⁄ 10 min, followed by 45 cycles of57 °C ⁄ 1 min, 95 °C ⁄ 30 s, and 45 °C ⁄ 29 s. All analyses wererun in triplicate. An 18S-rRNA ... promoter, was con-firmed by plasmid DNA NdeI digestion. Parental plasmidpHC1, lacking the G6PD-6PGL fragment was used as atransfection control. Plasmid DNAs were purified (PlasmidMaxi Kit, ... Prepstation(Applied Biosystems, Foster City, CA, USA). IsolatedRNA was reverse transcribed to cDNA using the High-CapacityÔcDNA Archive Kit (Applied Biosystems) as des-cribed by the manufacturer using specific...

10
437
0

Tài liệu Báo cáo khoa học: Malaria)the global disease docx

...

1
462
0

Tài liệu Báo cáo khoa học: ER stress and diseases docx

... path-way. CHOP ⁄ GADD153 (growth arrest and DNA damage 153) is a transcription factor, the expression ofwhich is induced by the ATF6 and PERK pathwaysupon ER stress [70,106,107]. CHOP– ⁄ – cells ... leucine zipper; c-Abl, Abelson murine leukemia viral oncogene homolog 1; C ⁄ EBP, CCAAT ⁄ enhancer-binding protein; CHOP, C ⁄ EBP-homologous protein; CREB, cAMP response element-binding protein; ... was originally identiﬁed as a pro-tein expressed in response to hypoxia. ER dnaJ(ERdj)1, ERdj3 ⁄ human ER-asociated dnaJ (HEDJ),ERdj4, ERdj5, SEC63, and p58IPK are ER chaper-ones belonging...