κb is required for the survival effect of tnf α in macrophages
Báo cáo y học: "Regulation of chondrocyte gene expression by osteogenic protein-1" ppt
... regulation of these two binding proteins by OP-1, which could mean that Gremlin and Follistatin perform distinct functions in the mediating BMP responses or they are involved in different stages of signaling ... bradykinin and bradykinin inducible receptor b1 [26] Therefore, it was of interest to examine expression of neuromediators and their receptors in the present array study After stimulation for 48 ... expression was inhibited by antisense oligonucleotides These results are consistent with previous data on the protein level in an in vivo model of disc herniation, where injection of OP-1 into the nucleus...
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Tài liệu Comparison of lung cancer cell lines representing four histopathological subtypes with gene expression profiling using quantitative real-time PCR pptx
... immunostaining of S100P in the cytoplasm and the nucleus, while SQ cells showed immunostaining of RAB25 in the cytoplasm The localization of S100P and RAB25 in tumor tissues was similar to that in ... if the amount of clinical tissue is limited, for instance when samples are obtained by microdissection of cancer cells While these issues are not problematic for analyzing lung cancer cell lines, ... this is the first report that systematically analyzed them together in all four subtypes The gene network was analyzed using Ingenuity Pathways Analysis software and is displayed graphically in...
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Báo cáo hóa học: " A quantitative real time PCR method to analyze T cell receptor Vb subgroup expansion by staphylococcal superantigens" doc
... phosphokinase C pathways [10], leading to a massive production of proinflammatory cytokines including interleukin-2 and interferon-g [11], resulting in extensive proliferation of T cells bearing ... observed for Vb subgroups consisting of a single gene, as well as G3PDH and Cb genes, showed single peak A representative result obtained for Vb1 is shown in Figure 1C The melting curve for the Vb1 ... primers used in qRT-PCR have uniform and high efficiency and linearity The specificity of qRT-PCR using SYBR Green I platform was often determined by analyzing melting curves In this study, the specificities...
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Báo cáo sinh học: " Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses" pdf
... it was the second most reliable reference gene in HEP2 cells infected with the same strain of HSV-1) These findings therefore highlight the importance of re-evaluating the choice housekeeping genes ... determining the suitability of 10 commonly used reference genes in the context of infection with various strains of HIV-1, HSV-1, CMV and VZV in a range of both primary and cultured cell lines using ... normalisers in QPCR we subjected the data to analysis using the GeNorm tool [13] (Table 2) Using the results from the GeNorm analysis it was possible to list the housekeeping genes in order of reliability...
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Báo cáo sinh học: " Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections" doc
... Corresponding to the recent estimation the SD of the CT value was highest for Act in of viruses, indicating that Act is no reliable reference gene in this setting In contrast, TBP and PPI displayed the ... BestKeeper indices; even actin was included into our gene panel These findings demonstrate the usefulness of analysing a wide variety of reference gene candidates The inconsistent data regarding to the ... 11.28), confirming to be the most inappropriate reference gene It is remarkable that the obtained BestKeeper index values are low, despite the inclusion of Act in the calculation Calculating BestKeeper...
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Báo cáo hóa học: " Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses" pot
... it was the second most reliable reference gene in HEP2 cells infected with the same strain of HSV-1) These findings therefore highlight the importance of re-evaluating the choice housekeeping genes ... determining the suitability of 10 commonly used reference genes in the context of infection with various strains of HIV-1, HSV-1, CMV and VZV in a range of both primary and cultured cell lines using ... normalisers in QPCR we subjected the data to analysis using the GeNorm tool [13] (Table 2) Using the results from the GeNorm analysis it was possible to list the housekeeping genes in order of reliability...
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báo cáo hóa học:" Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections" doc
... Corresponding to the recent estimation the SD of the CT value was highest for Act in of viruses, indicating that Act is no reliable reference gene in this setting In contrast, TBP and PPI displayed the ... BestKeeper indices; even actin was included into our gene panel These findings demonstrate the usefulness of analysing a wide variety of reference gene candidates The inconsistent data regarding to the ... 11.28), confirming to be the most inappropriate reference gene It is remarkable that the obtained BestKeeper index values are low, despite the inclusion of Act in the calculation Calculating BestKeeper...
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báo cáo khoa học: " Selection of reference genes for quantitative real-time PCR expression studies in the apomictic and sexual grass Brachiaria brizantha" ppt
... establishment of the minimum number of reference genes required for an accurate calculation of the relative expression of a target gene This ideal number is given by the inclusion of a certain number ... geometric mean of the expression values of the set of the control genes tested The lower the M value, the more stably expressed the gene is Also, the program enables the exclusion of the most unstable ... corresponds to the least stable expression in a set of samples As a result, the normalization factor (NF) is defined, by considering the M value of the most stable genes This information allows for the...
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báo cáo khoa học: " Validation of reference genes for quantitative real-time PCR during leaf and flower development in Petunia hybrida" pot
... fulfilment of the PhD degree of IM in the framework of the MSc-PhD program with Quality mention from the Spanish Ministry of Education MCD-2005-00339 Work performed in the lab of BH was funded by the ... order, with the aim of obtaining an optimal list of genes for each Petunia line The results of the merged data revealed that the most adequate of the genes tested for normalization in Mitchell ... genes are shown The number of genes required for a reliable quantification is established using a Pairwise Variation (PV) cut-off of 0.15; n is the the minimum number of control genes required NA...
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Báo cáo khoa học: "Detection of anatid herpesvirus 1 gC gene by TaqMan™ fluorescent quantitative real-time PCR with specific primers and probe" pps
... about the distribution of the different kinds of tissues AHV-1 attenuated vaccine can be distributed in various tissues and organs of ducks within hour by subcutaneous route in this study, furthermore, ... the copies of most tissues (other than kidney) reached a peak at hours postinoculation in AHV-1 Cha strain-infected ducks The concentration of nucleic acid in DNA vaccine-inoculated ducks maintained ... further investigate pcDNA3.1-gC DNA vaccine We are currently studying the dynamic distribution of gC in AHV-1-infected and DNA vaccine-inoculated ducks by using this method We believe that this...
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Báo cáo y học: "Endothelial Cytomegalovirus infection monitored by quantitative real-time PCR in critically ill patients" pps
... Viremia peaka Outcome 30 9,130 Discharged from ICU 141 63,400 Died in ICU 133 130,000 Discharged from ICU 105 137,000 Died in ICU ICU stay (days) Viremia peak is expressed in copies/ml DBSA, deep burn ... 2011, 15:412 http://ccforum.com/content/15/2/412 Page of Figure Cytomegalovirus plasma load measurements during ICU stay of four severe burn patients Table Patient characteristics Patient Age (years)...
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Báo cáo sinh học: "Quantitative real-time PCR study on persistence of pDNA vaccine pVax-Hsp60 TM814 in beef muscles" pptx
... usage of DNA vaccines in food- producing animals In this work, the QRTPCR method was used for the study of the persistence of pDNA at the injection sites in mice and beef cattle For this reason ... are necessary for the assessment of the risk of residual plasmid presence in consumable parts of DNA vaccinated livestock, mainly in muscles Nowadays, there are no definitive guidelines available ... pDNAX The samples of muscles were homogenised, weighted, frozen in liquid nitrogen and stored at -70°C until further processing Experiment II The influence of the age of the mice on the dynamics of...
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Quantitative real time PCR
... Stephen Bustin Abstract The MIQE (minimum information for the publication of quantitative real-time PCR) guidelines were published in 2009 with the twin aims of providing a blueprint for good real-time ... technical information inadequate for the purpose of assessing the validity of published qPCR data This had, and continues to have serious implications for basic research, reducing the potential for ... number of researchers aware of the existence of these guidelines as well as an increasing number of citations of the original publication in the peer-reviewed literature (> 2,200 by March 2014) There...
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Báo cáo y học: " Quantitative biomarker analysis of synovial gene expression by real-time PCR" potx
... is within the detection range of quantitative PCR The dashed line indicates the threshold cycle, C(t), for this assay The dark bar at C(t) indicates the range of C(t) of biopsies containing sufficient ... for small proof -of- concept clinical trials R358 Biomarker measurement in arthritis patients originally relied on protein analysis of fluid compartments Blood sampling is often included in clinical ... in the target tissue in RA, the synovium, which is a primary source of cytokines and effector molecules The first serial biopsy studies to assess the effect of therapeutic agents in RA used in...
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Luận văn :Định lượng kháng nguyên Cyfra 21-1 bằng kỹ thuật Real-time PCR
... Cytokeratine (theo thứ tự protein axit bazo), sợi trung gian nhóm III gồm protein axit dạng sợi desmin, vimentin glial Loại IV bao gồm sợi protein thần kinh (NF-L, NF-M NF-H) internexin, protein nhóm ... nhóm V gọi lamin nhân (chỉ cở nhân tế bào) Các protein cytokeratin lại xếp vào nhóm VI, gồm có felensin phakinin Cytokeratin biểu mô (sợi trung gian nhóm I II) hình thành trình phát sinh có liên ... Cancer: risk factors and prevention Lung Cancer Panel”, CA Cancer J Clin 48 (3): 167-176 11.Coulome PA, Omary MB (2002), “Hard and soft principle defining the structure, function and regulation of...
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Kỹ thuật Real time PCR chẩn đóan sớm HIV trên trẻ em
... để biết tình trạng huyết học thực trẻ sinh từ bà mẹ HIV(+) Nếu trẻ không phát sớm điều trị thuốc kháng virus Trẻ có nguy tử vong 50% 02 năm sống Kỹ thuật kinh điển chẩn đóan nhiễm HIV trẻ -RT PCR/b-DNA ... thấp trẻ sinh từ mẹ nhiễm HIV Agence Nationale de recherches sur le Sida et les hépatites virales Kỹ thuật « Real-time PCR » có độ nhạy, đặc hiệu cao, giá thành thấp so với kỹ thuật kinh điển, ... trẻ sinh từ mẹ nhiễm HIV Agence Nationale de recherches sur le Sida et les hépatites virales Tiếp cận kỹ thuật • Phương pháp: • So sánh phương pháp chẩn đoán HIV trẻ em • • Phương pháp kinh điển...
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Phát hiện và định lượng virus gây bệnh đốm trắng trên tôm sú bằng kỹ thuật Real - time PCR
... = 240 nm (theo Graindorge & Flegel, 1999)) (Nguồn: Bùi Quang Tề, 2003) 2.3.1.3 Đặc tính sinh học Virus đốm trắng ký sinh tổ chức ngoại bì, trung bì, mang, quan lymphoid biểu bì vỏ kitin Virus ... NudiBaculoviridae Theo hội nghị virus học quốc tế lần thứ 12 (2002), nhà khoa học phân loại virus gây bệnh đốm trắng giống Whisspovirus thuộc họ Nimaviridae (Tạp chí thơng tin KHCN Kinh Tế Thủy Sản, ... Bản, In- đơ-nê-xi-a Ấn Độ Trong thời gian 1994 – 1995, virus gây bệnh đốm trắng gây chết hầu hết tơm ni (P monodon; P indicus) dọc theo bờ biển phía Đơng Ấn Độ phía Tây Ấn Độ (Tạp chí thơng tin...
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Xây dựng quy trình định lượng các sản phẩm biến đổi gen bằng phương pháp Real-time PCR
... purpose, methods for GMOs detection and quantification in order to determine the percentage ratio of GMO presentation in food and feed need to be developed So, in this thesis, a process for GMO quantification ... Minh City, VietNam Aug 2005 ESTABLISHING A PROCESS OF GMOs (Genetically Modified Organisms) QUANTIFICATION BY REAL-TIME PCR METHOD Instructors: Le Dinh Don and Nguyen Thai Thuy Recently, GMO is ... method Evaluating quantification ability and efficiency of method Applying it for quantification some of food and feed derived from maize in Ho Chi Minh City Results: A process of GM maize quantification...
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XÂY DỰNG QUY TRÌNH PHÁT HIỆN ĐỘT BIẾN rtA181V/T VÀ rtN236T KHÁNG ADEFOVIR CỦA VIRUS VIÊM GAN B (Hepatitis B Virus) BẰNG KỸ THUẬT REAL-TIME PCR
... dùng INF để điều trị VGB mạn [6] Thuốc tăng cường miễn dịch không INF Tăng cường tế bào miễn dịch chống nhiễm tế bào T sản xuất INF tự nhiên thể Bao gồm: Theradigm, Thymosin α- 1 (Zadaxin), vacxin ... triển điều trị VGB gồm: Interferon (INF), chất tương tự nucleotide, thuốc kháng virus không nucleotide, thuốc tăng cường miễn dịch không INF Interferon Interferon họ protein tự nhiên, có hoạt tính ... L-Nucleotide gồm lamivudine (3TC), emtricitabine (FTC), telbivudine (L-dT) clevudine (L-FMAU) Nhóm II: acyclic phosphonate gồm adefovir (PMEA), tenofovir (PMPA) cidofovir (HPMPC) Nhóm III:...
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XÂY DỰNG QUY TRÌNH ĐỊNH LƯỢNG CYTOMEGALOVIRUS (CMV) TRONG MÁU, NƯỚC TIỂU BẰNG PHƯƠNG PHÁP REAL-TIME PCR
... - 75% Guanin+ Cytocine Với gen > 240.000bp, có tiềm mã hoá protein cho 200 khung đọc mở (opening reading frames) có khả tổng hợp khoảng 100 protein virus Hiện nay, xác định vài protein từ gen ... chloroform [2] Nguyên tắc: mẫu bệnh phẩm trộn với dung dịch phenol /chloroform /Izoamyl alcohol(P/C/I=25:24:1) protein mẫu bị phenol làm biến tính vón cục lại Khi ly tâm, nhờ có diện chloroform, ... (envelope) (hình 1.6) - Là lớp lipit kép gồm lipoprotein có 33 protein cấu trúc, vài protein cấu trúc glycoprotein (glycosylate) - Những phân tử glycoprotein nằm bề mặt có phần lồi Phần lồi giúp cho virus...
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