... carnitine: 1 mm (for standard comparison withother published data), and a concentration equal tothe Km for carnitine of each enzyme (for comparisonbetween mutants). In this article, we show ... sequence as being responsiblefor the pecu-liar kinetic characteristics of this enzyme, acting as anegative determinant for malonyl-CoA sensitivity.Asp17 may account, at least in part, for the highdegree ... nat-urally occurring variation, Glu17, isresponsiblefor both the low malonyl-CoA sensitivity and high carnitine affinity characteristics of the pig enzyme.This is the first demonstration that a single...
... active conformation, Val371 forms contactswith neighboring residues that are important for stabi-lizing the active state (e.g. Asp189, whichis part of theS1 pocket responsiblefor the binding ... substitution of Val371 toisoleucine might prevent the full development of theactive conformation. This hypothesis is further con-firmed by the results of FIX proteolytic assays, which showed a slight ... sequence identity of 30–45%), thisposition is occupied solely by a valine. This conservedamino acid is replaced by isoleucine in the Val371IleFXI mutant. Interestingly, an isoleucine residue natu-rally...
... factor that favors lysogeny and is responsiblefor primary control of the lambda develop-mental decision for lysis or lysogeny [4,7–9]. In theabsence of CIII, CII is rapidly degraded by the ATP-dependent ... proteolysis of r32by differentconcentrations of CIII or CIIIC. The bands corresponding to r32-C-his (1.2 lM) remaining after proteolysis with GST–HflB (0.8 lM) for 40 min in the presence of His6–CIII ... However, this proteolysis is muchslower in vitro, because of a lack of chaperone machin-ery. The in vitro proteolysis requires ATP [14]. Weexamined the proteolysis of purified r32-C-his (1.2...
... after medium renewal; 5Â,reoxygenated for 5 min after 7 h hypoxic incubation; 30Â, reoxygen-ated for 30 min after 7 h hypoxic incubation.Cdk2 is dispensable for replicon initiation D. Stabenow ... renewal; 5Â, reoxy-genated for 5 min after 7 h hypoxic incubation; 30Â, reoxygenated for 30 min after 7 h hypoxic incubation.D. Stabenow et al. Cdk2 is dispensable for replicon initiationFEBS ... bound Cdc6 is high at the end of the hyp-oxic period and distinctly decreases within 30 min after reoxygenation. The remainder is apparently convertedinto the slower migrating form. This significantdecrease...
... prior to hydrolysis. The low-affinity state wasobserved to be retained immediately post-hydrolysis. Therefore, the dissoci-ation of phosphate and ⁄ or ADP is likely to be responsiblefor resettingof ... high-affinityconformation for the transporter, whichis supportedby the restoration of high-affinity binding in the ADP-bound conformation. That this step of the catalyticcycle is associated ... of ATPper se is the ‘power stroke’ for drug translocation, andthat energy obtained from the hydrolysis process is used to reset the transporter. That ATP binding is responsiblefor the shift...
... subsequently distin-guished after a second amplification which allows discrim-ination between the alleles on the basis of the f ragment sizes(Fig. 8 , Step IIA).In the first step, a 266-bp (265 bp for ... first one is an ACRS-PCR technique [26], the principleof whichis to create a TaqI restriction site (TCGA) by usinga mismatching primer (C9LM*) which allows both the Fand M alleles to be discriminated ... 715/18 795/18 875 Da (18 555 Da after a lkaline phos-phatase hydrolysis), corresponding to that expected for theF variant. This result is the first evidence for the heterozy-gous status (M/F)...
... văn ở đây tạo điều kiện cho động thực vật sinh sôi nảy nở, thích hợp cho việc phát triển nguồn sinh vật trên cạn và dưới nước, thuận lợi cho canh tác nông nghiệp, cho phát triển thuỷ hải sản.-19- ... Định danh đã thể hiện khả năng tư duy của con người, giúp ích cho tư duy của con người. “Tri giác cảm tính cho ta sự vật, lí tính cho ta tên gọi sự vật” [55; 88]. Con người tạo ra ngôn ngữ bằng ... lịch sử, phong tục, tập quán xưa của một cộng đồng người. -34- dưới lực ép, không cho trỗi dậy, không cho nổi lên” của “dằn” [65; 236]) trong PNNB…Những gì được con người nhận thức giống...
... bronchitis B. chestnut C. chronometer D. chrisma8. A. chauvinism B. exchange C. teacher D. childrearing9. A. bristle B. indict C. Connecticut D. Vinci10. A. scherzo B. scholar C. school D. ... 4. A. ought B. bough C. thought D. brought5. A. spoor B. door C. floor D. force6. A. poisonous B. noise C. tortoise D. buoy7. A. golf B. adulthood C. wood D. bosom8. A. wool B. mood C. took ... happy D. plane6. A. drama B. aroma C. apology D. parliament Bảo Thoa 1 AN ANSWER KEY IS NOT WISHED FOR! Bảo Thoa 7 1. A. inversion B. permanent C. clerk D. derby [Br. E]2. A. teach B....
... bronchitis B. chestnut C. chronometer D. chrisma8. A. chauvinism B. exchange C. teacher D. childrearing9. A. bristle B. indict C. Connecticut D. Vinci10. A. scherzo B. scholar C. school D. ... special2. A. character B. cart C. chemistry D. chimney3. A. society B. social C. cellar D. proceed4. A. chain B. choice C. choir D. chocolate Bảo Thoa 2 WHICH SOUND IS DIFFERENT?These tests will ... 4. A. ought B. bough C. thought D. brought5. A. spoor B. door C. floor D. force6. A. poisonous B. noise C. tortoise D. buoy7. A. golf B. adulthood C. wood D. bosom8. A. wool B. mood C. took...
... that eitherone isresponsiblefor everything or one has refused responsibility.This is how Levinas answers those who say that to be responsible for everything is to be responsiblefor nothing.In ... on this account, butthat does not mean that the relationto being in which the ethical is situated is not crucial. This is whyit is not irrelevant what is said in the saying, just as it is not ... toward the concrete, which is where the encounter takes place. The point is not just toshow that such acts are impossible for an existence that is concernedonly for its own existence, as they would...