transport of ceramide between the endoplasmic reticulum and golgi apparatus
Báo cáo khoa học: Cytosolic phospholipase A2-a and cyclooxygenase-2 localize to intracellular membranes of EA.hy.926 endothelial cells that are distinct from the endoplasmic reticulum and the Golgi apparatus pdf
... constitutively present on the lumenal surfaces of the ER and on the inner and outer membranes of the nuclear envelope [14] Within the last decade, many groups have studied the relocation of cPLA2-a following ... 4B) of staining across the cell further demonstrated the overlap between the FITC (green) and DAPI (red) channels, suggesting that cPLA2-a was present on both the inner and outer surfaces of the ... cell The subcellular locations of these proteins also vary, with some being present in the ER, others in the nuclear membrane and some present at both these locations Thus the relocation of cPLA2-a...
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Tài liệu Báo cáo khoa học: Basis of recognition between the NarJ chaperone and the N-terminus of the NarG subunit from Escherichia coli nitrate reductase pdf
... 100-fold and the lifespan of the complex by 10-fold, as judged by koff The physiological chaperone cycle probably consists of the rapid binding of the N-terminus of the partner, regardless of whether ... enthalpy, independent of the buffer used in the experiment, and another term representing the contribution of the proton ionization of the buffer, which is multiplied by the number of protons that ... hypothesize that the protonated state of the chaperone initiates this cycle, whereas the deprotonated state occurs upon completion of the maturation process of the partner The nature of the signal...
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Báo cáo khoa học: Protein folding includes oligomerization – examples from the endoplasmic reticulum and cytosol doc
... closes the lid domain and drastically decreases the on and off rates of substrate from BiP One of the two nucleotide exchange factors then mediates the release of ADP, allowing the binding of ATP, ... as a demanding ER client Both folding of the subunits and assembly of IgM occur in the ER [238] The PDI family member ERp44 and the lectin ERGIC53 together function in the transport of assembled ... the control of the secreted amount of the complete complex by controlling the production of one of the subunits [245] Disulfide bonds are formed between the antibody subunits; they stabilize the...
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Báo cáo khoa học: The stop transfer sequence of the human UDPglucuronosyltransferase 1A determines localization to the endoplasmic reticulum by both static retention and retrieval mechanisms docx
... membrane expression [6] In the absence of positive transport signals, the localization of a protein in the ER may result from the properties of the TMD and its interaction with the membranes It has ... from the ER compartment to at least the medial Golgi apparatus and trans -Golgi apparatus, where complex sugars are added Analysis of CD4–TMD ⁄ CT protein on SDS ⁄ PAGE gave a single band of 44 ... has been proposed that the length of the TMD of Golgi and plasma membrane proteins was in part responsible for their subcellular localization To address whether the length of UGT1A TMD plays a...
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Báo cáo khóa học: Assembly of the silk fibroin elementary unit in endoplasmic reticulum and a role of L-chain for protection of a1,2-mannose residues in N-linked oligosaccharide chains of fibrohexamerin/P25 ppt
... significance of the assembly of the elementary unit of fibroin in ER It was demonstrated in this study that the elementary unit of fibroin, having the same molar ratio of H- and L-chains, and fhx/P25 as of ... composition of H6fhx1 [1] The disulfide linkage between H- and L-chains is not formed in Nd-s and Nd-sD mutants because of the lack of Cys-172 in the mutant L-chain [8] It is of interest to note that the ... [27] and to ensure the efficient intracellular transport and secretion of H-chains There seem to be two key events in the assembly of the (H-L)6fhx1-type elementary unit: (a) the formation of disulfide-linked...
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Báo cáo khoa học: Paralog of the formylglycine-generating enzyme – retention in the endoplasmic reticulum by canonical and noncanonical signals pptx
... lysates (C) and media (M) by western blotting Note that the aliquots of cells and medium were loaded at a ratio of 10 : The amount of total pFGE in cells and medium and the percentage of intracellular ... pFGE, and observed that it also conferred ER retention (Fig 4A) The PGEL tetrapeptide is accessible at the surface of the pFGE molecule The PGEL C-terminus of pFGE is located on the surface of the ... Fig The C-termini of the pFGE dimer are exposed at the surface of the molecule The ribbon model of the pFGE dimer 3D structure is shown, as determined through X-ray crystallography [25] The three...
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Characterization the role of kinectin in assembly of translation elongation complex to endoplasmic reticulum and its involvement in organelle motility
... surface then bids the tail of the melanocyte-specific spliced form of myosin Va The motor domain of this myosin then allows association of melanosomes with the actin network and leads to their ... (Gelfand and Bershadsky, 1991; Mandelkow and Mandelkow, 1995) The alignment of tubulin dimers within the microtubules leaves α-tubulins exposed to one end and β-tubulins exposed at the other, ... every eukaryotic cell They differ in the type of filament they bind to (either actin or microtubules), the direction in which they move along the filament, and the ‘cargo’ they carry Many motor...
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Tài liệu Báo cáo khoa học: Interruption of triacylglycerol synthesis in the endoplasmic reticulum is the initiating event for saturated fatty acid-induced lipotoxicity in liver cells pdf
... 5B) The ER is the site of synthesis of all secretory proteins and resident proteins of the membrane system, and any perturbation that compromises the proteinfolding capacity of the organelle ... phosphorylation of JNK when the two agents were coadministered (Fig 4C) Taken together, these results indicate that the location of the protective action of oleate was upstream of ER stress activation Whether ... continuously and accumulate them in the form of lipid droplets Moreover, coadministration of oleate restored the ability of stearatetreated cells to synthesize TAGs and prevented cell toxicity These...
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Báo cáo khoa học: Protein transport across the endoplasmic reticulum membrane pdf
... N-terminus on either side of the membrane, depending on the amino acid sequence of the protein, which often determines the orientation of subsequent TMs If the first TM is long and the preceding ... [16] The aqueous interior of the channel and its shape help to minimize the energy required for the translocation of a polypeptide through the membrane The plug can only return to the center of ... [12] The a-subunit consists of two halves, TMs 1–5 and TMs 6–10, which form a lateral gate at the front and are clamped together at the back by the c-subunit (Fig 4A) The 10 helices of the a-subunit...
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Báo cáo khoa học: Missense mutations as a cause of metachromatic leukodystrophy Degradation of arylsulfatase A in the endoplasmic reticulum potx
... during the pulse and chase periods, the others only during the chase periods In the experiments in which the secretion of newly synthesized enzymes was enhanced by the addition of NH4Cl, the drug ... the Golgi, so that newly synthesized lysosomal enzymes appear in the medium In the case of mutants, the appearance in the medium is thus an indicator that the enzyme has left the ER In none of ... for less than 5% of the enzyme synthesized during the pulse period Discussion Missense mutations are by far the most frequent type of mutations in the ASA gene [1] The effects of these mutations...
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Báo cáo Y học: Inhibition of the SERCA Ca21 pumps by curcumin Curcumin putatively stabilizes the interaction between the nucleotide-binding and phosphorylation domains in the absence of ATP pot
... 3A) and ATP (Fig 3B) in the absence and presence of curcumin, using the coupled enzyme assay In Fig 3A, the half-maximal activation of the ATPase by Ca2þ was measured in the absence of and in the ... sites and ligands, respectively, and [EL] is the concentration of bound ligand The total concentration of sites can be expressed as N[E]0, the product of total protein concentration ([E]0) and the ... side of the membrane While in the E2 form the Ca2þ binding sites have translocated across the membrane to the luminal side and are of low affinity Furthermore, in the E1 conformational state, the...
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Báo cáo khoa học: Cellular response to unfolded proteins in the endoplasmic reticulum of plants pptx
... was synthesized The expression pattern of o2 and fl-2 depends on the molecular basis of the mutation It remains necessary to evaluate the relationship between the expression patterns and the molecular ... and GLS2) were identified in the ER-luminal domain of ATF6 [85] ATF6 localizes to the ER through interaction between GLS1 and BiP In the absence of BiP, ATF6 is constitutively transported to the ... of proteins from the ER, and promote transport of newly synthesized proteins from the ER into ER subdomains or Golgi in yeast [167–170] Newly synthesized proteins that not fold correctly in the...
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Báo cáo Y học: Amino acids 3–13 and amino acids in and flanking the 23FxxLF27 motif modulate the interaction between the N-terminal and ligand-binding domain of the androgen receptor pdf
... The results are presented in Fig In the absence of ligand, none of the AR NTD proteins showed LBD interaction However, in the presence of ligand, both AR.NTDwt and AR.NTDD3– 13 bound to AR LBD with ... was lost The most obvious difference between both assays is the coupling of AR.NTD to GalAD in the yeast assay, and the absence of a second transactivation domain linked to AR NTD in the mammalian ... interaction with the charged clamp [5,36,37] The first and last leucine residue in the LxxLL motif enter the hydrophobic cleft in the LBD, and directly contact amino acid residues within the cleft The variable...
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Báo cáo hóa học: "Microrna profiling analysis of differences between the melanoma of young adults and older adults" pot
... each permutation, the p-values were re-computed and the number of genes significant at the 0.001 level was noted The significance level of the global test was the proportion of the permutations ... largest variance over the samples of all such orthogonal linear combinations, and so on The samples were first centered by their means and standardized by their norms, and then the multi-dimensional ... intergenerational and comparative microRNA profiling of the primary melanocytic neoplasms of adults and young adults We observed distinct miRNA profiles in the primary melanocytic neoplasms of adults and young...
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Báo cáo sinh học: " Typing of human rotaviruses: Nucleotide mismatches between the VP7 gene and primer are associated with genotyping failure" pdf
... differences between the target region of the respective genes and the primer sequences used for typing led to the genotyping failure [1,10,11,17] The Dhaka hospital of ICDDR,B, situated in the central ... if there was any mismatch between them Four mismatches were found in the Das G1 primer, 9T1-1, at the 5' end (Fig 2) Due to these mismatches, the Das G1 primer failed to detect most (75%) of the ... with the Gouvea primer set, the distribution of rotavirus G-types changed dramatically (Fig 1) Type G1 now accounted for 44.8% of the isolates and became the most prevalent genotype, and the number...
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Báo cáo hóa học: " Nucleotide mismatches between the VP7 gene and the primer are associated with genotyping failure of a specific lineage from G1 rotavirus strains" potx
... was reported that the accumulation of point mutations in VP4 and VP7 genes was the main cause of the failure of the G or P-typing of rotavirus strains [7,8,12,16-21] At least, there are four multiplex ... out of 121 G1 strains from lineage I showed the four specific mismatches at the 5' end of the 9T1-1 primer This was associated with the failure of genotyping of G1 strains from Bangladesh Two of ... change the priming binding site, in order to avoid the mismatches between the primer and the VP7 gene [7,17,20] However, the failure of the G-typing of rotavirus strains, detected during surveillance...
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báo cáo hóa học:" Typing of human rotaviruses: Nucleotide mismatches between the VP7 gene and primer are associated with genotyping failure" pptx
... differences between the target region of the respective genes and the primer sequences used for typing led to the genotyping failure [1,10,11,17] The Dhaka hospital of ICDDR,B, situated in the central ... if there was any mismatch between them Four mismatches were found in the Das G1 primer, 9T1-1, at the 5' end (Fig 2) Due to these mismatches, the Das G1 primer failed to detect most (75%) of the ... with the Gouvea primer set, the distribution of rotavirus G-types changed dramatically (Fig 1) Type G1 now accounted for 44.8% of the isolates and became the most prevalent genotype, and the number...
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báo cáo khoa học: "Genetic disequilibria between the α β-, κ-casein -, S1 and the β-lactoglobulin loci of the Bavarian Brown and Bavarian Simmental cattle" docx
... of represents the disequilibrium between loci i and j and p q the gene , ; j ij r denotes the gametic correlation and N equals the number at the loci of gametes in the sample In our samples the ... differentiation between the A’, Nand A alleles at the f3-Cn locus which however did not prevent the recognition of the disequilibria between the f3-Cn and the a,,-Cn loci In our tBV sample the disequilibrium ... found between the casein loci and the plactoglobulin locus The estimated disequilibria between casein loci are given in table as squares of the gametic correlations together with the sign of D...
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Báo cáo y học: " The interaction between the PARP10 protein and the NS1 protein of H5N1 AIV and its effect on virus replication" pot
... transcriptional profiling in influenza A virus-infected lung epithelial cells: The role of the nonstructural NS1 protein in the evasion of the host innate defense and its potential contribution to pandemic ... in BHK21 cells and the log growth period of the virus was between 36 h and 48 h, BHK21 cells were used as host cells of AIV After host cells were decided, we explored the effect of BHK21 cells ... PARP10 To verify the screening result of yeast two-hybrid system, we identified the in vivo and in vitro interaction between NS1 and PARP10 First, we verified the presence of the interaction with...
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Báo cáo y học: "Discrepancy between the in vitro and in vivo effects of murine mesenchymal stem cells on T-cell proliferation and collagen-induced arthritis" doc
... μl of PBS in the left ear DTH response was calculated as the percentage swelling (the difference between the increase of thickness of the right ear and the left ear, divided by the thickness of ... of iNOS and COX-2 in inhibition of T-cell proliferation was demonstrated by the addition of inhibitors of these enzymes - GW274150 and indomethacin [8,36], respectively - to the co-cultures The ... μg of CII in the right ear and vehicle in the left ear Delayed-type hypersensitivity responses were measured as the percentage of swelling (100 × [(thickness of the right ear – thickness of the...
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