... close to the twofold axis of E3preventing the association of a second PSBD [28,29]. The interaction occurs at the C2-axis of symmetry in the E3 dimer, chiefly with the interface domain of E3,which ... representing the PSBD ofthe dihydrosuccinyltransferase chain of the 2-oxoglutarate dehydrogenase complex of E. coli [12]and ofthe dihydroacetyltransferase chain ofthe PDHcomplex of B. stearothermophilus ... of Biochemistry, University of Cambridge, UK The 2-oxo acid dehydrogenase complexes consist of multiple copies of three distinct enzymes that togethercatalyse the oxidative decarboxylation of...
... fighting the fantasies of his mind, andtook another breath of air. This time it burned less, and he could force anawareness ofthe smells around him. But there was none ofthe pungentodor ofthe ... was supervising the supervisors, making an inspection tour of allthis. Of all what? Apparently then this must be another of their frenzied ef-forts to find a way to put back the sky. He'd ... the warlocks of this world had discovered that they could notsolve the problem ofthe sky, they must have gone into a state of purehysteria, like a chicken dashing back and forth in front of...
... response. The girls will think that if they do it just right, they’ll get the reaction they expect. It’s the same kind of instinct that keeps a grown woman going after a narcissistic or other-wise ... morning the sisters climbed on each other’s dressers to get to the clothes hanging in their closets. One girl had a pink two-piece outfit, and the other had a green two-piece outfit. Their mother ... adjoined each other. The sandbox was in our yard, and the swing set straddled the invisible line that divided our properties. Our mothers, who soon became friends, saw the advantage of their two...
... levels altered the ratio of APPsa to APPsb,but did not substantially modify the total levels of APPs or of APP CTFs. This suggests that there is adiscrete pool of FLAPP that is directed towardsprocessing, ... that is essential for generation ofthe amyloid b-protein is the b-site APP-cleaving enzyme 1 (BACE1). Here, we investigated the effects of genetic manipulation of BACE1 on the processing ofthe ... signaling role ofthe ICDsreleased by the APP family of proteins, but mayimpact on other functions of this family of proteins.Experimental proceduresReagentsUnless otherwise specified, chemicals...
... into his foreign dominions was the readiest way of converting them to the principles ofthe Christian religion. These, then, were the first forerunners in the great cause ofthe abolition ofthe ... awakened? What isthe meaning ofthe noise around us, ofthe trampling of people's feet, ofthe rustling ofthe bow, the quiver, and the lance? Let us rise up and inquire. Behold! the inhabitants ... nature of it; and ofthe extent of it; and ofthe difficulty of subduing it.—Usefulness also ofthe contemplation of this subject. I scarcely know of any subject, the contemplation of which is...
... ρn,dkthroughout the rest ofthe paper.Our key idea in the proof of Theorem 1 isthe use ofthe endomorphismbundle End(Vk) and the construction of sections of this bundle via Toeplitzoperators ... from the rest ofthe mapping tori as a purelyTQFT consequence of Corollary 1.In this paper we have initiated the program of using ofthe theory of Toeplitz operators on the moduli spaces in the ... that −16{ϑ∧[ϑ∧ϑ]} is a generator ofthe image ofthe integer cohomology Annals of Mathematics Asymptotic faithfulness ofthe quantum SU(n) representations ofthe mapping class groups...
... translocation ofthe periplasmic loops of KdpDin the absence and in the presence of CCCP. These resultsdemonstrate that the pmf is required for efficient membraneinsertion of KdpD with the tags. This is ... loop is cleaved by the protease, only a smallshift ofthe molecular mass ofthe protein is expectedbecause the antibody recognizes the remaining C-terminaldomain. In the presence of YidC, the ... that ofthe YidC-containingcells (Fig. 8B, compare lanes 2 and 5). Taken together, theseresults suggest that the presence ofthe epitope tag is the reason why KdpD-C requires the assistance of...
... S2and the location of structural elements(Fig. 4). Furthermore, the Rexterms are distributedthroughout the 3D structure ofthe protein, and most of them are large (Fig. 4); the exception is ... responsible, from a thermodynamic point of view, for the binding ofthe monomeric species of CAC?We have previously discussed the variation in the freeenergy of binding as a function ofthe changes ... (i.e. the internal motions ofthe bond vectorsare independent ofthe overall rotational movement of the molecule). Furthermore, the internal motions of each bond vector are independent of each other,...
... system of His15 and a distortion of the N-terminus ofthe first helix, which supports the histidinering. In ad dition, the C -terminal helix is bent because the sidechain of Leu86 located at the ... orientation, is arranged almost parallel to the a-helices and the b-sheet ofthe protein. As the 1HN-15Nbond vectors in the a-helices are therefore almost parallel to the z-axis, their size is mainly ... 69–82). The active site of the HPr molecule containing the residue His15 is formed byloop L1.Recalculation ofthe 3D-structure of wild-type HPrand comparison with the mutant proteinIt is known...
... water, ofthe very colour ofthe ocean itself, buoyed up their hopes ofthe discovery ofthe WesternPassage. Davis turned his ships to the south, coasting the shore. Here and there signs of man ... for his discovery ofthe Hudson river, was sent out by Sir John Wolstenholme and Sir DudleyDigges to find the North-West Passage. The story of his passage ofthe strait, his discovery ofthe ... progress of physical science, the rapidadvance in the knowledge of electricity and magnetism, and the rise ofthe science of biology were profoundlyaltering the whole outlook ofthe existing...
... regions of dis-order in the ABDs of spinophilin and neurabin. Asshown in Fig. 1, these programs also predicted a highdegree of disorder in the ABDs of spinophilin andneurabin. On the basis of these ... activity of spinophilin. The histo-grams on the right show a quantitative anal-ysis ofthe polymer length distributions of actin alone (control, top histogram) or in the presence of an equimolar ... NationalInstitute of Neurological Disorders and Stroke to WP. The content is solely the responsibility ofthe authorsand does not necessarily represent theof cial views of the National Institute of Neurological...
... used for the disintegration ofthe cells (data notshown). Because ofthe high expression levels of the transiently transfected receptors in COS7 cells, they wereused for the analysis of dimerization ... membrane-proximaldomains of gp130 is limited to ensure the correct spacingbetween the CBM and the membrane, each ofthe domainswas replaced by the corresponding domain ofthe GCSFR. The replacement is assumed ... II ofthe ligand contacts the CBM of gp130 [14,15],while site III contacts the Ig-like domain ofthe LIFR [13].In the resulting complex, D3 of gp130 and D5 ofthe LIFRshow the greatest distance...
... andpromiscuous sex. There was more to rock music than this, much more.In the musician's lyrics, from the classic rock music era ofthe seventiesto the more modern forms of rock music ofthe ... the essentials of music. Although there are modified forms of instrumentsand there are even new types of instruments, music still manages toconvey a message of one type or another to all that listen. ... Social issues also played a major role in the developing culture of the seventies. Bands often used their popularity to spread their feelingson such topics. They were, and still are, the central of...