the emerging role of micrornas in adult stem cells
Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 2
... Within the linear range of the assay (~5-25μg/mL), the more protein present, the more the Coomassie Blue binds changing the absorbance of the sample After getting cell lysates from the CHO cells, ... of Human SPHK Inhibitors components in the samples, which are then separated in the TLC plate The plate was run in the chamber until the solvent front reached 2cm from the top of the plate, then ... newly developed inhibitors, a better understanding of the role of SPHK in the process of stem cells differentiation will be studied as well In this chapter, only the compounds synthesis and evaluation...
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Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 3
... Staining 3.1.3.1 Alizarin Red S Staining Alizarin Red S staining is widely used to evaluate the calcium-rich deposits generated by cells In this study, the staining was used to detect the mineralization ... vs cock) However, in cells cultured in the presence of 1μM of DMS, 1μM of CP6, and 5μM of CP6, there was no increase in osteocalcin expression, compared the cells grown without inhibitors (cock+1 ... shown in Figure 3.4D In this type of AD-MSCs, the presence of 0.5μM of DMS increased the staining in both 14 days and 28 days culture as compared with the osteogenic-induced sample without inhibitors...
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Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 4
... the presence of S1P did not show any side-effect on maintaining stemness of the cells However, the amount of cells that were proliferated in the S1P serum-free, or S1PPDGF-AB combined culture ... retained their differentiation potentials Due to the limited number of the stem cells, only Alizarin Red S Staining was used to quantify the osteogenic differentiation Cells were first seeded in ... for a total of 28 days, then fixed and stained with Alizarin Red S The Alizarin Red S Staining quantification results are shown in Figure 4.11 Alizarin Red Staining quantification of human BM-MSCs...
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Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 5
... transplanting them into the body, by committing stem cells along specific differentiation path More work, including in vivo work, needs to be done to fully validate the potential for SPHK inhibitors in ... osteopenia, where the bone volume is decreased but the adipose tissue increases (Jaiswal et al., 2000) It also suggests the potential for the use of SPHK inhibitors in preconditioning stem cells before ... differentiation in both types of MSCs in a dose-dependent manner The reason for why at high concentrations CP6 and DMS inhibited the stem cells osteogenic differentiation remains unclear In the future,...
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Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 6
... "Essential roles of sphingosine-1-phosphate and platelet-derived growth factor in the maintenance of human embryonic stem cells. " Stem Cells 23(10): 1541-8 Peng, H and J Huard (2003) "Stem cells in the ... “Characterization of N, N-dimethyl-derythro-sphingosine-induced apoptosis and signaling in U937 cells: independence of sphingosine kinase inhibition.” Prostaglandins & Other Lipid Mediators 86(1-4):18-25 ... et al (1998) “N,N-Dimethylsphingosine Is a Potent Competitive Inhibitor of Sphingosine Kinase but Not of Protein Kinase C: Modulation of Cellular Levels of Sphingosine 1-Phosphate and Ceramide.”...
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Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation
... increasing need for more MSCs is one of the main motivations of the present study, in particular, in studying the role of S1P in adult stem cell proliferation Another major motivation is the pressing ... activation, while HDL could inhibit all of these, by inhibiting the SPHK activity triggered by TNFα These findings suggested an atherogenic role for SPHK activation Interestingly, Nofer et al (2004) reported ... overcomes this limitation of prolonged culture duration, is modulating the genome of the stem cells by using recombinant DNA constructs, encoding for the expression of certain proteins or growth factors...
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The role of microRNAs in embryonic stem cell development and differentiation
... as in embryonic carcinoma (EC) cells, which may have conserved roles in mammalian pluripotent stem cells (2) microRNAs expressed specifically in ESCs but not in other cells including EC cells These ... differentiate into all cell types in the body Although the clinical use of adult stem cells, which are present in multiple tissues in the mammalian body, is attractive due to the lack of allogenecity, adult ... noncoding RNAs similar to the siRNAs involved in RNA silencing, originate from stem- loop precursors in the genome They have been shown to play important roles in diverse processes including apoptosis,...
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báo cáo hóa học:" Emerging role of microRNAs in diagnosis and treatment of various diseases including ovarian cancer" pdf
... loss of dopaminergic neurons, it could be possible that miRNAs play a role in the disease pathogenesis These investigators [8] hypothesized that inducing alterations in the miRNA networks of the ... mutations can result in inherited forms of Parkinson's disease [7] Kim et al [8] suggest that miRNAs are essential for maintaining the dopaminergic neurons in the brain Given that Parkinson's disease ... Recently, there has been considerable interest in understanding the mechanism of the RNA regulatory phenomena and how miRNA functions in carcinogenesis Specifically, determining how the regulation of...
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The role of language in adult education and poverty reduction in Botswan
... English at the expense of the other languages that exist in the country, the adult language program in Botswana maintains the hegemony and the gap between the poor and the rich, the major and minority ... value system of the dominant group Since competence in English is a pre-requisite for participation in the national political and economic system, the majority of citizens in Botswana have remained ... dominates and the vast majority of people are left out In an education system where English dominates the majority of learners get little benefit from schooling, either in terms of acquiring the necessary...
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Báo cáo hóa học: " The emerging role of insulin-like growth factor 1 receptor (IGF1r) in gastrointestinal stromal tumors (GISTs)" pdf
... the intracellular tyrosine kinase In theory, if they work both should block receptor activation, and thereby block the intracellular pathways Of course, direct inhibition of the molecules of these ... IGF1Rinhibitors and the effect of the combination with standard front line therapies [52] These analyses are particularly necessary to confirm the putative oncogenic role of IGF1R in WT GISTs Indeed ... pathogenetic role of IGF1R in WT GISTs, the IGF axis blockade may be beneficial in the treatment of GIST However, in- depth analysis of the IGF axis in GISTs is mandatory, since ligand signaling could...
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an investigation into the role of chemokines in haemopoietic stem cell quiescence
... 1 AN INVESTIGATION INTO THE ROLE OF CHEMOKINES IN HAEMOPOIETIC STEM CELL QUIESCENCE Amy Sinclair BSc (hons), MRes Submitted in fulfilment of the requirements for the degree of Doctor of Philosophy ... identification of stem cells marked an important discovery in the field of biology and medicine The haemopoietic system represents an important component in the stem cell time line, beginning with ... important roles throughout development, with the migration of HSC to different sites of haemopoiesis and in the adult at basal levels In the adult, the egress of HSC into the periphery is modulated in...
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Investigation of the role of microRNAs in spinocerebellar ataxia type 3
... proteins that play an important role in maintaining protein homeostasis in the cell by aiding the process of proper folding of proteins and thereby preventing the accumulation of misfolded proteins ... 2009) whereas the C-terminal domain has ubiquitin interacting motifs (UIMs) which define the specificity of the Josephin domain to cleave ubiquitin chains having linkages at Lys63 (Winborn et al, ... mRNA (b) The initial exons code for the Josephin domain in the ATXN3 protein, the rest of the exons code for the C-terminal domain with the polyQ chain encoded by the 10th exon (c) Introduction...
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Báo cáo khoa học: "The key role of semantics in the development of large-scale grammars of natural language" pdf
... results in trimming something off something else; in the case of example (9) above trimming the bush results in trimming the dry branches off the bush And this is what the semantic type in (16) ... Impingement Predicates In order to capture the semantic properties of the arguments of the most representative verb of the impingement predicates class in German, the verb schlagen (hit) in examples ... argument of the embedded stateof-affairs Finally, as far as direct arguments are concerned, in Koenig and Davis (2000) these are predicted to link off the value of the KEY attribute 3.1 The verbs...
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CITY DIPLOMACY: THE EXPANDING ROLE OF CITIES IN INTERNATIONAL POLITICS potx
... happens: from the rise of the internet and the spread of McDonalds to the establishment of the International Criminal Court and the emergence of global terrorism Focusing on the implications of globalization ... as the municipality of Amsterdam, understand the importance of preventing this, and stress the necessity of a local international policy being in line with the international policies of other involved ... gain as well from maintaining a certain image and attracting foreign capital An interesting aspect of this diplomatic game is the theory and practice of city branding – the notion of applying...
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Báo cáo khoa học: Analyzing the catalytic role of Asp97 in the methionine aminopeptidase from Escherichia coli potx
... mutants of the MetAP-I from E coli S Mitra et al affect the DG value for the binding of the rst metal ion; however, the entropic factor (TDS) for the binding of the rst metal ion decreases in the ... Co(II)-binding site, and indeed bidentate binding of Glu97 may prevent binding of the solvent ligand that appears to be present in other mono-cobalt(II) species of EcMetAP-I Combination of these ... metal-binding site, but also probably assists in binding and positioning the substrate through interactions with the N-terminal amine The 6256 data reported herein highlight the complexity of the...
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The functional role of emotions in aesthetic judgment pot
... of nerves and networks in the brain, involving several areas near the edge of the cortex concerned with instinct and mood This area of the brain is intricately involved in motivation and basic ... about through the interrelationship of the outcomes of basic emotional systems (in the appraisal process), that elicit aesthetic emotions, and the process of learning in the course of interaction ... depict the dynamic functions of emergent representation and of the general learning process, which are playing a primary role in the synthesis of Bickhard’s Interactivist model them The visual system...
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aging of the genome the dual role of dna in life and death mar 2007
... started systems thinking, thinking about a system as the emergent property of the interaction among all the components of the system and not as mere aggregates of its parts System thinking in biology ... being inherited together, whereas the farther away they are from each other, the more chance of their INTRODUCTION being separated by the process of crossing over The Morgan is now the unit of ... seen, in the history of biology the discovery of the logic of life was followed by an understanding of the logic of aging Following Weismann’s original non-adaptive concepts of explaining aging,...
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Báo cáo y học: " The potential role of appetite in predicting weight changes during treatment with olanzapine" ppt
... interpreting the results in a clinical context, and revised the manuscript VPH was involved in study design and conduct of the clinical studies, in design of the current analyses, contributed to interpreting ... or undergoing study procedures Page of Study design Included in the analyses were patients from prospective, phase IV clinical trials examining the efficacy and safety of olanzapine in adult (18 ... gain during antipsychotic drug therapy [15] An understanding of the role of appetite changes in weight gain during antipsychotic treatment would be helpful to clinicians and patients, some of...
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Báo cáo y học: "The expanding role of Tax in transcription" pdf
... resulting in the loss of the capping machinery and the association of splicing, elongation and chromatin remodeling factors In the case of HTLV-I, Tax has been shown not to associate with a CTD kinase ... or other cdk kinases) may aid in increased Tax transactivation In this context, HTLV-I infected cells contain increased levels of cyclin E/cdk2 kinase activity, through sequestration of cdk inhibitor, ... to interact with ATF-4 bound to the 21bp repeats, enhance the binding of ATF-4 to the enhancer, and recruit CREB binding protein (CBP) to the viral promoter [5] Recently, CREB1 and ATF-4, in...
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Báo cáo y học: "cGlobal analysis of microRNA target gene expression reveals the potential roles of microRNAs in maintaining tissue identity" ppsx
... important roles for determining the timing of tissue differentiation during larva period of Drosophila development and maintaining the tissue identity during the adulthood It should be noted that the ... understanding the global role of miRNAs in maintaining lineage of differentiated tissues and cells has great impact on the studies of miRNAs in cancer genetics 16 Methods Datasets used in this study The ... genes These findings strongly suggest that miRNAs play a most important role in driving tissue terminal differentiation and maintaining tissue identity rather than in determining or regulating...
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