0

the big tug of war sleep from 2 through 3 years

The Gale Encyclopedia of Neurological Disorders vol 2 - part 3 pptx

The Gale Encyclopedia of Neurological Disorders vol 2 - part 3 pptx

Sức khỏe giới tính

... Washington, DC 20 0 02- 424 2 (20 2) 33 6-60 13; Fax: (20 2) 21 8 -35 99 kcooke@apa.org Dawn Cardeiro, MS, CGC S Neurosarcoidosis Central nervous system (CNS) involvement can affect the pituitary ... and fills the inner portion of the eyeball between the lens and the retina Atrophy The progressive wasting and loss of function of any part of the body Iritis Inflammation of the iris, the membrane ... in the back of the head Description The greater and lesser occipital nerves run from the region where the spinal column meets the neck (the suboccipital region) up to the scalp at the back of the...
  • 51
  • 540
  • 0
Ho chi minh was the communist leader of north vietnam from the end of world war II until his death in 1969

Ho chi minh was the communist leader of north vietnam from the end of world war II until his death in 1969

Kỹ thuật - Công nghệ

... reported to be September 3 [21 ] as not to coincide with the National Day Recently the government changed his official death day to September 2[ 22] [ 23 ] The former capital of South Vietnam, Saigon, ... and in the United States during World War I before going to France near the end of the war There he became involved in the French socialist movement and was (1 920 ) a founding member of the French ... revolutionary leader of the twentieth century Ho Chi Minh led the communists of Vietnam in their efforts to drive out the forces of Japan in the 1940s (see World War II), France in the 1950s (see Dienbienphu),...
  • 20
  • 604
  • 0
Tài liệu An Aviator''''s Field Book Being the field reports of Oswald Bölcke, from August 1, 1914 to October 28, 1916 doc

Tài liệu An Aviator''''s Field Book Being the field reports of Oswald Bölcke, from August 1, 1914 to October 28, 1916 doc

Cao đẳng - Đại học

... Tetschen, at the border, I was relieved of the bother of customs officials through the kindness of an Austrian officer It was the lasting grief of my companion that he had to submit to the customs ... about a battle in the air is nonsense A part of his propeller broke off and, due to the jerk, the wire braces of the fuselage snapped The fuselage then broke off Aside from the great personal ... meters west of A OCTOBER 22 , 1916 11:45 Several of my men and I headed off two enemy biplanes coming from the east Both fell The one I attacked was shot apart OCTOBER 22 , 1916 About 3: 40 in the afternoon...
  • 38
  • 565
  • 0
Tài liệu Báo cáo khoa học:

Tài liệu Báo cáo khoa học: "Learning the Latent Semantics of a Concept from its Definition" pptx

Báo cáo khoa học

... 50. 02 69 .30 70.08 71.44 69 .30 71.45 Adv 58 .2 62. 1 66.1 67.1 62. 8 67.1 58.90 62. 85 67.97 67.05 62. 85 67.05 Verb 21 .6 30 .8 33 .2 34 .9 37 .1 39 .9 33 .5 41.4 43. 7 43. 9 47.4 48.8 24 .6 39 .5 40.5 41 .2 40.0 ... jcn 1 43 dim 50 75 100 125 150 SE2 57.4 - 60.5 57.8 - 60 .3 58.6 - 60.5 58 .2 - 60 .2 58 .2 - 59.8 SE3 52. 9 - 54.9 53. 5 - 55 .2 53. 5 - 55.8 53. 9 - 55.5 53. 6 - 54.6 SE07 43. 1 - 44 .2 43. 3 - 44.6 43. 7 - ... jcn+elesk jcn+wmfvec Noun 43. 9 63. 5 68.6 69.7 69 .3 70.8 39 .9 58.5 58.1 61.5 60.5 61 .2 27.4 47 .2 49.7 52. 2 52. 8 53. 5 40. 13 61.04 63. 15 64.64 69.61 70.64 Adj 43. 6 63. 9 60 .2 64.5 63. 9 64.5 44.1 57.7 60.8...
  • 5
  • 585
  • 0
Tài liệu Báo cáo khoa học: Dimer asymmetry and the catalytic cycle of alkaline phosphatase from Escherichia coli doc

Tài liệu Báo cáo khoa học: Dimer asymmetry and the catalytic cycle of alkaline phosphatase from Escherichia coli doc

Báo cáo khoa học

... Fig The influence of Mg2+ on the kinetic properties of APase from E coli The influence of Mg2+ on the kinetic properties of APase from E coli in the presence of a Zn2+ to dimer ratio of : in 2A2M1P ... Zn2+ to dimer ratio of : [Mg2+] (M) KS1 (mM) KS2 (mM) Vm (lmolÆmin)1) b – 2. 1 · 10)6 2. 1 · 10)5 2. 1 · 10 )3 0.08 ± 0.01 0.07 ± 0.01 0.08 ± 0.01 0.08 ± 0. 02 1. 72 ± 0.78 2. 56 ± 3. 31 2. 03 ± 0. 53 2. 51 ... increases from 0. 32 , in the absence of Zn2+, to 7 .26 lmol pNPÆmin)1 in the presence of six Zn2+ ions per dimer A further increase of the Zn2+ ion concentration to a Zn2+ to dimer ratio of : and...
  • 9
  • 590
  • 0
Tài liệu Báo cáo Y học: Identification of a set of genes involved in the biosynthesis of the aminonucleoside moiety of antibiotic A201A from Streptomyces capreolus pdf

Tài liệu Báo cáo Y học: Identification of a set of genes involved in the biosynthesis of the aminonucleoside moiety of antibiotic A201A from Streptomyces capreolus pdf

Báo cáo khoa học

... Pur4 AtaP5/ Pur5 AtaP7/ Pur7 AtaP10/ Pur10 26 8 27 3 427 429 22 8 22 8 1 72 1 52 36 1 33 8 % Similarity % Identity 76.8 72. 9 80.5 74.9 73. 1 65.6 70.6 65.0 61 .3 55 .2 sequences were proposed to have phosphatase, ... (pIJ7 02) Dpur10 (pIJ7 02) Dpur10 (pSEXP0 .2) Dpur10 (pA2A10) Dpur4 (pIJ7 02) Dpur4 (pSEXP4 .2) Dpur4 (pA2A4) Dpur5 (pIJ7 02) Dpur5 (pFV8) Dpur5 (pA2A5) 3 .29 1.68 0. 03 0.89 0.95 0. 02 0. 32 0 .22 0.00 0 .35 ... elucidation of the function of oleP1, oleG1 and oleG2 Ó FEBS 20 02 Aminonucleoside A201A biosynthetic genes (Eur J Biochem 26 9) 5 535 from the oleandomycin biosynthetic gene cluster of Streptomyces...
  • 9
  • 728
  • 0
Tài liệu Báo cáo Y học: Steady-state kinetics of the glutaminase reaction of CTP synthase from Lactococcus lactis The role of the allosteric activator GTP in coupling between glutamine hydrolysis and CTP synthesis potx

Tài liệu Báo cáo Y học: Steady-state kinetics of the glutaminase reaction of CTP synthase from Lactococcus lactis The role of the allosteric activator GTP in coupling between glutamine hydrolysis and CTP synthesis potx

Báo cáo khoa học

... mechanism of the bienzyme complex J Biol Chem 27 6, 20 38 720 39 6 15 Kishore, N., Tewari, Y.B & Goldberg, R.N (20 00) A thermodynamic study of the hydrolysis of 1-glutamine to (1-glutamate + ammonia) and of ... Fig 1A The power value of the baseline and its 34 A 32 30 10 12 14 Time (min) 33 B 32 -1 where the latter reaction takes place only in the presence of UTP [1] Levitzki and Koshland originally suggested ... exceeding the time required for the experiments (about 20 40 min) The values of DH were found to be )29 .7 0.8 kJặmol)1 and )47 .3 0 .3 kJặmol)1 for the glutaminase reaction and the CTP synthesis...
  • 8
  • 698
  • 0
Tài liệu THE ECONOMIC FEASIBILITY OF ETHANOL PRODUCTION FROM SUGAR IN THE UNITED STATES docx

Tài liệu THE ECONOMIC FEASIBILITY OF ETHANOL PRODUCTION FROM SUGAR IN THE UNITED STATES docx

Cao đẳng - Đại học

... (tons) 23 ,5 02 27, 538 20 ,894 20 ,9 92 22, 134 22 , 529 25 ,1 62 28,0 72 24,810 25 , 131 27 ,5 13 28 ,20 3 29 ,1 43 26 ,24 9 31 ,8 53 28 ,065 26 ,680 29 ,886 32 , 499 33 , 420 32 , 541 25 ,764 27 ,707 30 ,710 30 , 021 27 , 537 13. 8 12. 0 ... value) 25 ,5 82 26,165 28 ,449 27 ,20 1 26 ,008 26 ,877 28 , 936 28 , 026 28 ,479 28 ,069 26 ,475 28 ,960 28 ,8 73 29 , 635 29 ,404 29 , 137 27 ,687 30 ,0 03 32 , 7 43 33, 577 34 ,29 1 32 , 775 33 ,9 03 31,9 42 27 ,24 3 24 , 726 10.66 ... 1980/81 3 , 23 4 2, 987 6 ,22 1 1981/ 82 3, 318 2, 804 6, 122 19 82/ 83 2, 6 92 3 ,26 3 5,955 19 83/ 84 2, 837 3, 0 73 5,910 1984/85 2, 915 3, 025 5,940 1985/86 2, 988 3, 136 6, 124 1986/87 3, 6 53 3,506 7,159 1987/88 3, 822 3, 425 ...
  • 78
  • 605
  • 0
Báo cáo khoa học: Crystal structures of the apo form of b-fructofuranosidase from Bifidobacterium longum and its complex with fructose pot

Báo cáo khoa học: Crystal structures of the apo form of b-fructofuranosidase from Bifidobacterium longum and its complex with fructose pot

Báo cáo khoa học

... Asp54 Od2 Asn 53 Nd2 2. 96 3. 06 2. 69 2. 73 2. 86 2. 85 2. 54 2. 66 2. 64 3. 09 3. 08 3 .22 2. 93 3.01 2. 65 2. 82 3. 14 2. 73 2. 58 2. 72 2.67 2. 97 3. 15 3 .22 O1 O3 O2 O4 B O5 maps in both monomers (Fig 3B) The conformation ... 24 2. 3 ⁄ 4 42 ⁄ 24 2. 0 ⁄ 456 ⁄ 28 1.9 ⁄ 4 53 ⁄ 26 2. 1 ⁄ 4 23 ⁄ 29 – 2. 0 ⁄ 422 ⁄ 29 2. 1 ⁄ 408 ⁄ 25 2. 1 ⁄ 417 ⁄ 26 2. 0 ⁄ 415 ⁄ 28 2. 5 ⁄ 459 ⁄ 24 2. 0 ⁄ 422 ⁄ 29 – 2. 0 ⁄ 468 ⁄ 35 2. 5 ⁄ 459 ⁄ 28 2. 4 ⁄ ... 457 ⁄ 26 2. 3 ⁄ 4 42 ⁄ 24 2. 1 ⁄ 408 ⁄ 25 2. 0 ⁄ 468 ⁄ 35 – 2. 6 ⁄ 448 ⁄ 23 2. 7 ⁄ 4 52 ⁄ 24 2. 0 ⁄ 456 ⁄ 28 1.9 ⁄ 4 53 ⁄ 26 2. 1 ⁄ 416 ⁄ 26 2. 0 ⁄ 414 ⁄ 28 2. 5 ⁄ 461 ⁄ 28 2. 4 ⁄ 457 ⁄ 27 2. 6 ⁄ 449 ⁄ 23 2. 6...
  • 17
  • 521
  • 0
Báo cáo khoa học: Solution structure of the catalytic domain of RICH protein from goldfish pot

Báo cáo khoa học: Solution structure of the catalytic domain of RICH protein from goldfish pot

Báo cáo khoa học

... T 236 38 0 34 0 30 0 26 0 22 0 180 0.1 Residue Number Δchem shift (p.p.m.) C T 236 0.5 V3 32 V3 32 G 335 0 .3 T 32 2 T 236 38 0 34 0 30 0 26 0 22 0 180 0.1 Residue Number E F D241 0 .3 F 239 D241 F 239 0 .2 38 0 34 0 30 0 ... The Authors Journal compilation ª 20 07 FEBS G Kozlov et al Solution structure of the RICH catalytic domain Δchem shift (p.p.m.) A B 0.7 T 32 2 T 236 0.5 V3 32 D241 F 239 0 .3 V3 32 A319 G 335 T 32 2 T 236 ... mutagenesis to investigate the role of 1608 20 21 22 23 24 25 26 27 28 29 30 31 32 33 cysteine and histidine residues in enzymatic activity J Biol Chem 27 6, 14804–148 13 Kozlov G, Lee J, Elias D,...
  • 10
  • 325
  • 0
THE ECONOMIC FEASIBILITY OF ETHANOL PRODUCTION FROM SUGAR IN THE UNITED STATES pot

THE ECONOMIC FEASIBILITY OF ETHANOL PRODUCTION FROM SUGAR IN THE UNITED STATES pot

Cao đẳng - Đại học

... (tons) 23 ,5 02 27, 538 20 ,894 20 ,9 92 22, 134 22 , 529 25 ,1 62 28,0 72 24,810 25 , 131 27 ,5 13 28 ,20 3 29 ,1 43 26 ,24 9 31 ,8 53 28 ,065 26 ,680 29 ,886 32 , 499 33 , 420 32 , 541 25 ,764 27 ,707 30 ,710 30 , 021 27 , 537 13. 8 12. 0 ... value) 25 ,5 82 26,165 28 ,449 27 ,20 1 26 ,008 26 ,877 28 , 936 28 , 026 28 ,479 28 ,069 26 ,475 28 ,960 28 ,8 73 29 , 635 29 ,404 29 , 137 27 ,687 30 ,0 03 32 , 7 43 33, 577 34 ,29 1 32 , 775 33 ,9 03 31,9 42 27 ,24 3 24 , 726 10.66 ... 1980/81 3 , 23 4 2, 987 6 ,22 1 1981/ 82 3, 318 2, 804 6, 122 19 82/ 83 2, 6 92 3 ,26 3 5,955 19 83/ 84 2, 837 3, 0 73 5,910 1984/85 2, 915 3, 025 5,940 1985/86 2, 988 3, 136 6, 124 1986/87 3, 6 53 3,506 7,159 1987/88 3, 822 3, 425 ...
  • 78
  • 523
  • 1
PRINCIPLES FOR THE RISK ANALYSIS OF FOODS DERIVED FROM MODERN BIOTECHNOLOGY

PRINCIPLES FOR THE RISK ANALYSIS OF FOODS DERIVED FROM MODERN BIOTECHNOLOGY

Sinh học

... the Guideline for the Conduct of Food Safety Assessment of Foods Derived from Recombinant-DNA Plants (CAC/GL 45 -20 03) , the Guideline for the Conduct of Food Safety Assessment of Foods Produced ... (CAC/GL 46 -20 03) and the Guideline for the Conduct of Food Safety Assessment of Foods Derived from Recombinant-DNA Animals (CAC/GL 68 -20 08) See footnote Reference is made to the Compilation of Codex ... recognised that there are other applications of product tracing These applications should be consistent with the provisions of the SPS and TBT Agreements The application of product tracing to the areas...
  • 3
  • 576
  • 0
Báo cáo khoa học: The crystal structure of annexin Gh1 from Gossypium hirsutum reveals an unusual S3 cluster Implications for cellulose synthase complex formation and oxidative stress response potx

Báo cáo khoa học: The crystal structure of annexin Gh1 from Gossypium hirsutum reveals an unusual S3 cluster Implications for cellulose synthase complex formation and oxidative stress response potx

Báo cáo khoa học

... output; b P31 12 61.05, 61.05, 21 5 .36 40 2. 1 808 32 0 27 4 12 100% (100%) 0.0 43 (0. 433 ) 24 054 (37 50)/ 26 56 (419) 4– 32 1 25 49 181 /3 0 .21 9 (0 .30 0)/ 0 .28 0 (0 .37 7) 51.9 87.5/11.5/1.0 Rfree defined in [35 ] Structures ... Anx24(Ca 32 ) AnxA5 Arg80-Glu99 Glu1 13- Arg271 CO117-Arg276 Asp39-Arg118 Arg276-Asp280 – Glu116-Arg2 72 CO 120 -Arg277 Asp96-Arg 121 Arg277 28 1 – Glu1 12- Arg271 – Asp 92- Arg117 Arg276-Asp280 groove on the ... (20 02) Dimerization of cotton fiber cellulose synthase catalytic Ó FEBS 20 03 25 64 A Hofmann et al (Eur J Biochem 27 0) 12 13 14 15 16 17 18 19 20 21 22 23 24 25 subunits occurs via oxidation of...
  • 8
  • 475
  • 0
Báo cáo Y học: Implications of the simultaneous occurrence of hepatic glycolysis from glucose and gluconeogenesis from glycerol pdf

Báo cáo Y học: Implications of the simultaneous occurrence of hepatic glycolysis from glucose and gluconeogenesis from glycerol pdf

Báo cáo khoa học

... concentrations of fructose [24 ] The generation of 3H2O from [2- 3H]glucose provides a good measure of the rate of hepatic phosphorylation of glucose in vitro [2, 25] Incubation of hepatocytes with [2- 3H]glucose ... (mM) [DHAP] [Gro -3- P] [ATP] 0.5 1.0 2. 0 3. 0 4.0 5.0 0.09 0. 13 0 .20 0 .24 0 .24 0 .28 2. 25 3. 93 7.09 7.89 7.87 8.44 2. 14 1.71 1 .29 1 .24 0.98 0.80 ‹ ‹ ‹ ‹ ‹ ‹ 0.01 0.01 0.01 0. 02 0. 02 0. 02 ‹ ‹ ‹ ‹ ‹ ‹ ... 0. 02 ± 0. 52 ‹ 0. 02 0. 02 ‹ 0.01 ± 0.11 ‹ 0.01a 0. 03 ‹ 0.01 ± 0. 13 ‹ 0.02a 0.58 ‹ 0. 03 ± 0.65 ‹ 0. 03 2. 52 ‹ 0.14 13. 18 ‹ 0 .26 11 .29 ‹ 0 .33 a,e P < 0.001 and P < 0.05, respectively, for the e€ect of...
  • 6
  • 328
  • 0
Báo cáo khoa học: The phosphate site of trehalose phosphorylase from Schizophyllum communeprobed by site-directed mutagenesis and chemical rescue studies pptx

Báo cáo khoa học: The phosphate site of trehalose phosphorylase from Schizophyllum communeprobed by site-directed mutagenesis and chemical rescue studies pptx

Báo cáo khoa học

... (20 08) 9 039 13 ê 20 08 The Authors Journal compilation ê 20 08 FEBS C Goedl and B Nidetzky 31 32 33 34 35 36 37 38 39 The phosphate site of Schizophyllum commune trehalose phosphorylase the bacterial ... Propargylamine 2, 2 ,2- Triuoro ethylamine 10.6 10.6 9 .2 8 .2 5.7 46.5 65.0 25 .4 75.8 79.5 )0.64 )0.15 )1 .38 )0. 43 0 .27 0.61 10 24 0.9 14 6.7 16 39 1.5 23 (24 )b 11 ( 122 )b a V [E] measured in the absence ... Acetamidine Aminoguanidine 13. 6 13. 4 13. 3 12. 5 11.0 59.1 79.5 98.1 67.7 71 .3 )1. 63 )1.16 )0.67 )2. 52 )1.99 0. 42 19 5 .2 5.4 2. 0 1.6 45 12 13 4.8 3. 8 K512A pKa Molecular volume [A3] logP V [E] [10)4ặs)1]a...
  • 11
  • 428
  • 0
Báo cáo khoa học: The crystal structure of pyruvate decarboxylase from Kluyveromyces lactis Implications for the substrate activation mechanism of this enzyme ppt

Báo cáo khoa học: The crystal structure of pyruvate decarboxylase from Kluyveromyces lactis Implications for the substrate activation mechanism of this enzyme ppt

Báo cáo khoa học

... 4199– 420 9 ª 20 06 The Authors Journal compilation ª 20 06 FEBS X11 MARCCD 0.8 125 100 180 0.5 26 5.5 P21 a ¼ 78. 72, b ¼ 20 3. 09, c ¼ 79.78, b ¼ 91. 82 0.40 99.0 2. 26 (2. 32 2. 26) 549 4 32 114 899 4.8 20 .2 ... diphosphate Mg2+ Water molecules 23 .58 2. 26 (2. 32 2. 26) 18 466 16 776 15.8 (16 .3) 21 .4 (27 .0) 0.015 1.477 92. 5 21 .7 22 .9 13. 4 15.8 29 .7 Neither the terminal residues, nor residues 105–1 13 in all subunits ... adjacent to the loop are labeled The orientation of the subunit is the same as that of subunit B in Fig 420 2 FEBS Journal 27 3 (20 06) 4199– 420 9 ª 20 06 The Authors Journal compilation ª 20 06 FEBS...
  • 11
  • 374
  • 0
Báo cáo khoa học: The subcellular localization of vaccinia-related kinase-2 (VRK2) isoforms determines their different effect on p53 stability in tumour cell lines pdf

Báo cáo khoa học: The subcellular localization of vaccinia-related kinase-2 (VRK2) isoforms determines their different effect on p53 stability in tumour cell lines pdf

Báo cáo khoa học

... Lazo PA (20 04) Human vaccinia-related kinase (VRK1) activates the 25 02 18 19 20 21 22 23 24 25 26 27 28 29 ATF2 transcriptional activity by novel phosphorylation on Thr- 73 and Ser- 62 and cooperates ... of the Caenorhabditis elegans genome using RNAi Nature 421 , 23 1 23 7 FEBS Journal 27 3 (20 06) 24 87 25 04 ª 20 06 The Authors Journal compilation ª 20 06 FEBS 25 01 Differential stabilization of p 53 ... Genet 33 , 39 6–400 31 Alarcon-Vargas D & Ronai Z (20 02) p 53- Mdm2 – the affair that never ends Carcinogenesis 23 , 541–547 32 Woods DB & Vousden KH (20 01) Regulation of p 53 function Exp Cell Res 26 4,...
  • 18
  • 333
  • 0
Báo cáo khoa học: Structure of the substrate complex of thymidine kinase from Ureaplasma urealyticum and investigations of possible drug targets for the enzyme pdf

Báo cáo khoa học: Structure of the substrate complex of thymidine kinase from Ureaplasma urealyticum and investigations of possible drug targets for the enzyme pdf

Báo cáo khoa học

... B-value (A2) P21 a ¼ 57.16 b ¼ 115.65 c ¼ 64.47 b ¼ 101. 02 tetramer 2. 00 (2. 11 2. 00) 99.1 (98.5) 9.0 (48.4) 16.4 (2. 9) 27 4 598 54 3 12 ESRF, ID14eh3 0. 931 100 19.5 23 .5 0.008 1.04 32 . 9 the flexible ... 46 21 18 14 52 35 44 34 196 170 95 80 77 43 21 17 30 52 15 15 76 48 52 22 19 15 Reference 33 70 20 10 [11,19], [19] [11,19], [19] [19] [29 ] [11,19], [29 ] [19] [19] Source of the compounds: a Sigma-Aldrich; ... 128 (Fig 2) The methyl group is positioned in a hydrophobic pocket lined by Cb of Ser1 63, Sd in Met21, and Cd1 in Leu 124 The closest polar atom is the carbonyl oxygen of residue 126 O3¢ of the...
  • 8
  • 392
  • 0
Báo cáo khoa học: Kinetics of the quinone binding reaction at the QB site of reaction centers from the purple bacteria Rhodobacter sphaeroides reconstituted in liposomes docx

Báo cáo khoa học: Kinetics of the quinone binding reaction at the QB site of reaction centers from the purple bacteria Rhodobacter sphaeroides reconstituted in liposomes docx

Báo cáo khoa học

... liposomes (Eur J Biochem 27 0) 46 03 21 58 22 23 59 24 25 26 27 28 29 30 60 31 32 33 34 35 36 61 62 isolated from thermophilic bacterium Chloroflexus aurantiacus Eur J Biochem 20 2, 625 – 634 Kutuzov, M.A., ... association of the RC 46 02 F Milano et al (Eur J Biochem 27 0) Ó FEBS 20 03 limit on the release rate of quinol, which will lead to a 52 and quinone is the diffusion of the latter through the proteoliposomes, ... diffuse towards and away from the enzyme, and the large volume of the bilayer allows the accommodation a large number of ligands; (b) the arrangement of the lipid molecules around the RC is not...
  • 11
  • 365
  • 0
Báo cáo khoa học: Tracking interactions that stabilize the dimer structure of starch phosphorylase from Corynebacterium callunae Roles of Arg234 and Arg242 revealed by sequence analysis and site-directed mutagenesis doc

Báo cáo khoa học: Tracking interactions that stabilize the dimer structure of starch phosphorylase from Corynebacterium callunae Roles of Arg234 and Arg242 revealed by sequence analysis and site-directed mutagenesis doc

Báo cáo khoa học

... 0. 03/ 3 .2 ± 0.1 2. 95 ± 0.10/stableb R 234 A R242A ± 3/ n.a 3. 8 ± 0.4/n.a 2. 60 ± 0.06 /20 ± 2. 00 ± 0. 02/ 12 ± 0.5 4.45 ± 0.05/stableb 2. 93 ± 0. 02/ stableb + phosphatea 5 .2 (3. 45 3. 55 2. 27 ± ± ± ± 0.2c/stableb,c ... verify the role of arginines as stabilizing elements of dimer contact regions [24 ,25 ] Therefore, irrespective of the exact orientation of Arg 234 , Arg 236 , and Arg2 42 at the TOWER interface region of ... inhibition of quenching of PLP fluorescence in the R 234 A and R242A mutants in the presence of phosphate at levels of 10 mM and 20 mM, relative to a control that did not Ó FEBS 20 03 21 32 R Griessler...
  • 11
  • 444
  • 0

Xem thêm