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single cell enzymatic dissociation of hesc lines oxf1 oxf4 and culture in feeder free conditions

Báo cáo khoa học: The enzymatic activity of SR protein kinases 1 and 1a is negatively affected by interaction with scaffold attachment factors B1 and 2 pot

Báo cáo khoa học: The enzymatic activity of SR protein kinases 1 and 1a is negatively affected by interaction with scaffold attachment factors B1 and 2 pot

Báo cáo khoa học

... form in living cells and that cell- extracted SAFB-bound kinases are inactive in vitro SRPK ⁄ SAFB complexes are present in the nucleus of HeLa cells Finally, we sought to detect the existence of ... additional N-teminal domain In our pursuit of a biological consequence of this interaction, we examined the activity of SRPK1a in the presence of SAFB1 and showed that the kinase is inhibited by ... domain RS domains are known to be protein–protein and protein–RNA interaction surfaces and the phosphorylation of serines affects those interactions as well as interactions between RS domain-containing...
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báo cáo hóa học:

báo cáo hóa học: " Quality of life among adolescents with sickle cell disease: mediation of pain by internalizing symptoms and parenting stress" pdf

Hóa học - Dầu khí

... defining characteristics of SCD, pain serves as the cornerstone in explaining HRQOL in child, adolescent, and adult samples.[7] Our findings provide partial support for this focus on pain in ... internalizing symptoms of anxiety and depression) and family functioning (i.e disease-related parenting stress) in the association of pain with HRQOL If these concomitant pain variables are indeed ... physical and psychosocial domains of HRQOL.[3,7,8] However, the connection of pain with functioning across domains of HRQOL in SCD is not firmly established In the biobehavioral model of pain, a...
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Báo cáo y học:

Báo cáo y học: " The use of partial exchange blood transfusion and anaesthesia in the management of sickle cell disease in a perioperative setting: two case reports" pot

Báo cáo khoa học

... his assistance in editing this manuscript Authors’ contributions RJ was involved in conceiving, designing and writing this manuscript MT was involved in providing a description of the perioperative ... of units of red blood cells 5.3 0.25 Debridement humerus, transfusion of unit of red blood cell 4.4 0.21 General anaesthesia to insertion of central venous line 3.9 0.18 Transfusion of unit of ... exacerbation of SCD, such as hypoxia, hypovolemia, hypothermia and hyperviscosity, were prevented by monitoring his vital signs and maintaining them within tight ranges Anaesthesia from the beginning...
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Effect of Coagulant on Phosphorus Uptake and Release in EBPR Process

Effect of Coagulant on Phosphorus Uptake and Release in EBPR Process

Môi trường

... the setting to expect instability of phosphorus removal The volume of each reactor was L Two liters of synthetic wastewater mainly containing acetate and glucose (components are listed in Table ... shown in Fig In the anaerobic phase, PO4-P concentration increased to a certain value and remained constant in each run In Run1-1, PO4-P concentration in the anaerobic phase was higher than that in ... Journal of Water and Environment Technology, Vol 8, No.4, 2010 Phosphorus removal in SBR1 The PO4-P concentration in the effluent of SBR1 in Run1-1 to Run1-4 is shown in Fig In Run1-1, the mean and...
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Tài liệu Seasonal variation in the incidence of preeclampsia and eclampsia in tropical climatic conditions ppt

Tài liệu Seasonal variation in the incidence of preeclampsia and eclampsia in tropical climatic conditions ppt

Sức khỏe phụ nữ

... incidence of preeclampsia based on the timing of delivery In our study, we intend to establish if any such association exists in the distinct climate of the tropical coastal city of Mumbai, India ... had no influence on the incidence of preeclampsia In contrast, lower temperature, higher rainfall and humidity and lower barometric pressure were related to the triggering of seizures in patients ... factors in hypertensive disorders, vaginal bleeding and premature rupture of membranes during pregnancy Gynecol Obstet Invest 1991, 32(2):88-90 Makhseed M, Musini VM, Ahmed MA, Monem RA: Influence of...
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The Anatomy of Design: Uncovering the influences and inspiration in modern graphic design

The Anatomy of Design: Uncovering the influences and inspiration in modern graphic design

Mỹ thuật

... uncovering the influences and inspirations in modern graphic design steven heller and mirko ilic´ packaging and unpackaging design Graphic design is a composite of many influences and inspirations ... used as a kind of cityscape symbolizing the over-arching control of a benign faceless power over the quality of human life While the fingerprint is a random composition of contoured lines, which ... with the logo of French luxury brand Louis Vuitton rest in a farm in the rural area of China's capital Beijing Delvoye maintains a staff of local farmers and tattoo artists raising sows to use...
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CA AMERICAN STIOB: Or,What Late-Socialist Aesthetics of Parody Reveal about Contemporary Political Culture in theWest potx

CA AMERICAN STIOB: Or,What Late-Socialist Aesthetics of Parody Reveal about Contemporary Political Culture in theWest potx

Mỹ thuật

... Under such labor conditions, and often against their best intentions, journalists often find themselves cutting and pasting their own work and that of others, recycling extant segments of text to keep ... that instead of scrutinizing the complex meanings of social and political issues at stake in the elections, media channels focused all their efforts and ingenuity on representing the elections in ... traveled very, very far, and a lot of the articles were sympathetic and brought Bhopal and Dow into the subject again and again and again So I think probably dozens of articles that wouldn’t...
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Báo cáo khoa học: Selectivity of pyruvate kinase for Na+ and K+ in water/dimethylsulfoxide mixtures docx

Báo cáo khoa học: Selectivity of pyruvate kinase for Na+ and K+ in water/dimethylsulfoxide mixtures docx

Báo cáo khoa học

... monovalent cations and Mg2+ in pyruvate kinase, we determined the kinetics of saturation for Na+ and K+ in presence of 1.35 and 6.46 mM Mg2 +free (Fig 3) With 6.46 mM Mg2 +free, the Kapp and V for Na+ ... Solvation and energetic of binding of Na+ and K+ to pyruvate kinase It has been hypothesized [18] that the difference between Na+ and K+ in the activation and inhibition of pyruvate kinase could ... monovalent and divalent cation binding sites (Fig 2, inset) Competition between Na+ or K+ and Mg2+ + + Fig Effect of Na (A) and K (B) on the activity of pyruvate kinase in 100% water and in various...
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Báo cáo Y học: Functional reconstitution of the HIV receptors CCR5 and CD4 in liposomes pot

Báo cáo Y học: Functional reconstitution of the HIV receptors CCR5 and CD4 in liposomes pot

Báo cáo khoa học

... protein, they were incubated with cells expressing this glycoprotein complex Fig 10 Binding of soluble gp 120 or cells expressing gp120/gp41 to proteoliposomes containing CD4 and CCR5 A: Binding of ... expression vector (Invitrogen, Groningen, the Netherlands) in order to obtain a recombinant protein containing the C-myc epitope followed by six histidines at the C-terminus A plasmid containing the wild ... removal of detergent from mixtures of free and micellar detergent and mixed protein/detergent and lipid/ detergent micelles Upon removal of detergent, the protein will likely become insoluble and...
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Diagnosis of pulmonary tuberculosis by smear microscopy and culture in a tertiary health care facility: Biology and Medicine docx

Diagnosis of pulmonary tuberculosis by smear microscopy and culture in a tertiary health care facility: Biology and Medicine docx

Sức khỏe giới tính

... Passive case finding is the mainstay of case finding in most developing countries This relies heavily on sputum examination by smear and culture, chest radiology and tuberculin skin testing Under ... efficiency of conventional ZN staining method and culture on LJ medium in the detection of AFB in sputum samples of patients with pulmonary tuberculosis Keywords: Acid fast bacilli; culture; Lowenstein ... efficiency of ZN staining method and Culture on LJ medium Materials and Methods The study was approved by the local ethical committee and Informed consent was obtained from patients A total of 210...
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Report of the Sub-Committee of the Central Board of Directors of Reserve Bank of India to Study Issues and Concerns in the MFI Sector doc

Report of the Sub-Committee of the Central Board of Directors of Reserve Bank of India to Study Issues and Concerns in the MFI Sector doc

Ngân hàng - Tín dụng

... “margin cap” of 10% in respect of MFIs which have an outstanding loan portfolio at the beginning of the year of Rs 100 crores and a “margin cap” of 12% in respect of MFIs which have an outstanding ... banks and financial institutions including SIDBI As at 31st March 2010, the aggregate amount outstanding in respect of loans granted by banks and SIDBI to NBFCs operating in the Microfinance ... falling into three main groups a) The SHG-Bank linkage Model accounting for about 58% of the outstanding loan portfolio b) Non-Banking Finance Companies accounting for about 34% of the outstanding...
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A review on the cd content of rice daily cd intake and accumulation in the kidneys

A review on the cd content of rice daily cd intake and accumulation in the kidneys

Môi trường

... Daily intake of cadmium and its concentration in the renal cortex Over 100 µg/g of cadmium in the renal cortex was also reported in the 1970s and 1980s in Japan 268 in 1970 and 182 g/day in 1994 ... humans Interrelationships in food and feces and interactions in the liver and the renal cortex Keio J Med 1977; 26: 63–78 Ministry of Agriculture, Forestry and Fisheries Food Balance Sheet, Minister’s ... Cadmium, zinc, copper, and metallothionein levels in the kidney and liver of inhabitants of upper Silesia (Poland) Int Arch Occup Environ Health 1993; 65: 57–63 5) Nilsson U, Schütz A, Skerfving S,...
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Báo cáo khoa học: EmbR, a regulatory protein with ATPase activity, is a substrate of multiple serine⁄threonine kinases and phosphatase in Mycobacterium tuberculosis doc

Báo cáo khoa học: EmbR, a regulatory protein with ATPase activity, is a substrate of multiple serine⁄threonine kinases and phosphatase in Mycobacterium tuberculosis doc

Báo cáo khoa học

... whole cell lysate for 10 min, before incubation with resinbound EmbR The effect of kinase inhibitor was investigated by pre-incubating the whole cell lysate with mm staurosporine The effect of anti-PknH ... pathogenicity are in progress and are expected to provide intriguing insights into the significance of corresponding signaling events in M tuberculosis 2718 Experimental procedures Bacterial culture and growth ... DNAbinding activity of EmbR, the dephosphorylated form of EmbR was incapable of binding the promoter regions of emb genes at low concentrations, in agreement with the belief that EmbR itself in...
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Social Justice, Health Disparities, and Culture in the Care of the Elderly doc

Social Justice, Health Disparities, and Culture in the Care of the Elderly doc

Sức khỏe người cao tuổi

... Pierre, and Hilliard of shared symbols, beliefs, and customs that shape individual and group behavior and provide guidelines for speaking, doing, interpreting, and evaluating one’s actions and reactions ... in timing of diagnosis of the disease; and (4) disparities in access to care to treat the disease Although each of these issues is distinct, they are interconnected in creating and perpetuating ... First, knowing and understanding the culture of a group provides a conceptual and methodological “blueprint” to follow Ward Goodenough’s work on culture informs the elements of this blueprint and can...
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Báo cáo khoa học: Differential involvement of protein kinase C alpha and epsilon in the regulated secretion of soluble amyloid precursor protein docx

Báo cáo khoa học: Differential involvement of protein kinase C alpha and epsilon in the regulated secretion of soluble amyloid precursor protein docx

Báo cáo khoa học

... Biochem 271) Fig Secretion of sAPPa following PMA treatment of SYwt, SYa4 and SYDe neuroblastoma cells Incubation of the cells for h in the presence of increasing concentrations of PMA (10 nM, 100 nM, ... protein quantification Normalization of protein loading on each blot was obtained by loading a volume of sample of conditioned medium standardized to total cell lysate protein concentration Proteins ... downstream of muscarinic receptors is complex and involves the activation of ERKs and the MAP-kinase pathway we studied whether the downregulation of PKCe in uences the activation of MEK in our cellular...
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Báo cáo khoa học: Role of the surface charges D72 and K8 in the function and structural stability of the cytochrome c6 from Nostoc sp. PCC 7119 potx

Báo cáo khoa học: Role of the surface charges D72 and K8 in the function and structural stability of the cytochrome c6 from Nostoc sp. PCC 7119 potx

Báo cáo khoa học

... E68 and Y76 Interestingly, one of the side chain oxygens of D72 appears to be hydrogen-bonded to the backbone N atom of G6, bridging the crossing between the N- and C-terminal a helices of cyt ... energy of unfolding at [urea] (DGU0) In the mutant series, the overall height of the energy barrier for the rate-determining step of the folding ⁄ unfolding transition is mainly determined by ... of D72 by alanine one of the residues E68 or Q69 ‘takes over’ in forming a stabilizing hydrogen bond to an amino acid within a reoriented N-terminal a helix However, any such interpretation of...
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beyond the roof of the world music prayer and healing in the pamir mountains nov 2008

beyond the roof of the world music prayer and healing in the pamir mountains nov 2008

Vật lý

... is an increased interest in spirituality and religion (Levin 1997) that links directly with the public and academic interest of including aspects of spirituality and religiosity in medicine (Larson ... losing weight, gaining strength, mending, transforming, or healing broken relationships, achieving and maintaining desired emotional and spiritual states, transforming aspects of worldview, and ... of suffering, illness, joy, and pain and how are prayers offered at these different times—with sound or music, in silent meditation, by singing, chanting, intoning, vocalizing, weeping, wailing,...
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Báo cáo hóa học:

Báo cáo hóa học: " Impact of Video Coding on Delay and Jitter in 3G Wireless Video Multicast Services" ppt

Báo cáo khoa học

... Associate Professor, he joined Nagoya Institute of Technology, in which he is now a Professor in the Department of Computer Science and Engineering, Graduate School of Engineering From June 2000 to ... Computer Science and Engineering, Graduate School of Engineering, Nagoya Institute of Technology, Japan He has extensive research, development, and consulting experience in the area of telecommunications ... algorithms, and QoS control He is a Member of the IEEE, ACM, Information Processing Society of Japan, the Institute of Image Information and Television Engineers, and the Virtual Reality Society of Japan...
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Báo cáo lâm nghiệp:

Báo cáo lâm nghiệp: "Some methodological aspects of the National Forest Inventory and Monitoring in Slovakia" pptx

Báo cáo khoa học

... standing and lying deadwood in forest ecosystems has become more and more significant and hence, its detection was included within almost all NFIs in Europe The assessment methods for obtaining ... types will be used, and for monitoring the changes in the quality structure of the forest stands relative proportions of trees in individual quality classes will be determined Information about ... necessary information vary between the countries; they differ in the definitions of individual parts of this wood, in the lower limit from which it is recorded, and in detection details While in the...
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Báo cáo lâm nghiệp:

Báo cáo lâm nghiệp: "Variation of the tree form factor and taper in European larch of Polish provenances tested under conditions of the Beskid Sądecki mountain range (southern Poland)" docx

Báo cáo khoa học

... Śląska) and provenance (Pelplin) (Fig 10) The comparison of means, using the analysis of variance, showed that when the effect of dbh and height was eliminated the mean values of tapering of individual ... dimensions, and differences in its value may result from differences in the rate of tree growth of individual provenances (Kulej 2001) To eliminate their influence the coefficient of tapering zr was ... (Allen 1993) In such a case possible differences in the values of the form factor may be a result of differences in the diameter and height of trees of individual provenances To eliminate these...
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