reaction generation of reactive oxygen species and oxidative stress after acute is
Báo cáo sinh học: "Combination of cyclosporine and erythropoietin improves brain infarct size and neurological function in rats after ischemic stroke" potx
... Inflammatory Reaction, Generation of Reactive Oxygen Species, and Oxidative Stress after Acute IS Abundant studies have shown that innate immune mechanisms are rapidly activated following acute arterial ... between group and group (Figure 4D) Results Effect of Combined CsA and EPO on Infarction Area and Neurological Function after Acute IS Enhanced Reduction of Apoptosis and Oxidative Stress by Combined ... CsA and EPO therapy are equally effective in protecting the brain against ischemic damage after acute IS through suppressing inflammation, generation of ROS, and oxidative stress Of importance is...
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báo cáo hóa học:" Airborne particulate matter PM2.5 from Mexico City affects the generation of reactive oxygen species by blood neutrophils from asthmatics: an in vitro approach" docx
... Medicine and Toxicology 2009, 4:17 Figure by neutrophils in contact with PM2.5 In vitro generation of reactive oxygen and nitrogen species In vitro generation of reactive oxygen and nitrogen species ... modification of molecules and/ or disruption of cellular structures and tissue injury [9] Due to high MPO activity, the generation of hypochlorous acid (HOCl) and reactive nitrogen species (RNS) ... susceptibility of lipids to oxidation was used to calculate the sample size According to the mean comparison formula [15] with a standard deviation of 157.53 and a difference of 616, Za of 95% and a Zb of...
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báo cáo khoa học: " Differential patterns of reactive oxygen species and antioxidative mechanisms during atrazine injury and sucrose-induced tolerance in Arabidopsis thaliana plantlets" ppsx
... xenobiotic and ROS signalling are discussed Results Patterns of accumulation of singlet oxygen, superoxide radical and hydrogen peroxide The transfer of plantlets after weeks of growth to control and ... photoinhibition and photooxidative stress in Arabidopsis thaliana Plant Cell 2005, 17(12):3451-3469 Matysik J, Alia , Bhalu B, Mohanty P: Molecular mechanisms of quenching of reactive oxygen species ... component of the reactive oxygen gene network of Arabidopsis [67] Involvement of sAPX in response to oxidative stress has also been reported by transcriptional induction in the presence of H2O2,...
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Báo cáo khoa học: Antioxidant defences and homeostasis of reactive oxygen species in different human mitochondrial DNA-depleted cell lines pot
... Determination of intracellular reactive oxygen species as function of cell density Methods Enzymol 352, 91–100 44 Zuo, L & Clanton, T.L (2002) Detection of reactive oxygen and nitrogen species in tissues ... from the reaction of 5,5¢-dithiobis-(2-nitrobenzoic acid) with GSH In this reaction, GSH is oxidized to GSSG, which is then reconverted to GSH in the presence of glutathione reductase and NADPH ... mechanisms for energy supply and for maintenance of an appropriate redox environment [17,18] Analysis of q0 cells has provided insights into oxygen metabolism [13,17,19–21] and the role of mitochondria...
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Báo cáo y học: " Reduced levels of reactive oxygen species correlate with inhibition of apoptosis, rise in thioredoxin expression and increased bovine leukemia virus proviral loads" potx
... Control % of 8-OHdG positive B cells Red CM-XROS 60 Profile of fluorescence intensities 50 40 30 20 10 Control Figure of Analysis DNA oxidative damage Analysis of DNA oxidative damage After 24 h of ... Figure Generation of ROS in B lymphocyte mitochondria Generation of ROS in B lymphocyte mitochondria Confocal microscopy analysis of ROS production in B cells isolated from BLV-infected and control ... with apoptosis (Fig 1C) and negatively with proviral loads (Fig 6) Non-B cells Figure of Analysis thioredoxin expression Analysis of thioredoxin expression A) Western blot analysis of thioredoxin...
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Báo cáo y học: "Rheumatoid peripheral blood phagocytes are primed for activation but have impaired Fc-mediated generation of reactive oxygen specie" pptx
... the most studied acute- phase protein and is a good measure of activity of disease because high circulating levels are correlated with the acute inflammatory stages of the disease, and low levels ... Analysis of reactive oxygen species Analysis of the intracellular release of ROS was determined with DHR-123 Purified neutrophils were incubated with DHR123 before specific stimulation, and the ... effects of gender on loss of ROS generation Discussion We have demonstrated that there is a defect in the cooperative effect of FcγRIIa and FcγRIIIb in mediating ROS generation in neutrophils This...
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báo cáo khoa học: " Reactive oxygen species and transcript analysis upon excess light treatment in wild-type Arabidopsis thaliana vs a photosensitive mutant lacking zeaxanthin and lutein" pps
... Unraveling the tapestry of networks involving reactive oxygen species in plants Plant Physiol 2008, 147:978-984 39 Apel K, Hirt H: Reactive oxygen species: Metabolism, oxidative stress, and signal transduction ... footprints disclose specificity of reactive oxygen species signaling in Arabidopsis Plant Physiol 2006, 141:436-445 37 Miller G, Shulaev V, Mittler R: Reactive oxygen signaling and abiotic stress ... mutant and repressed in wild-type plants (Table 5) It is well known that the transcription and the translation of PSII and PSI genes is extremely complex and often uncoupled Analysis of the barley...
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Báo cáo khoa học: Muramyl-dipeptide-induced mitochondrial proton leak in macrophages is associated with upregulation of uncoupling protein 2 and the production of reactive oxygen and reactive nitrogen species docx
... infections, potentiation of anti-tumour activity of macrophages, manipulation of cytokine release and restoration of haematopoiesis [18–20] The parent molecule of this family is muramyl dipeptide ... induces reactive oxygen and nitrogen species production and upregulates UCP2 protein level, whereas MB does not We further show that the activity of UCP2 is consistent with the level of free ... inability of MB and LPS to induce any impairment in mitochondrial function after h of treatment RCR and states 2, 3, and FCCP rates of MB- and LPStreated cells were the same as those of unstimulated...
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Báo cáo khoa học: Structure, regulation and evolution of Nox-family NADPH oxidases that produce reactive oxygen species pptx
... chain of this residue forming hydrogen bonds with oxygens of the 3-phosphate; NH1 and NH2 of Arg105 form hydrogen bonds with the 4-OH and 5-OH of the inositol moiety; and the aromatic ring of Tyr59 ... through direct coordination of oxygen to the heme iron [33,34] This outer sphere (or peripheral) mechanism is consistent with the ‘two bis-histidyl heme ligation’ structure, and explains well why gp91phox ... regulation and evolution of Nox H Sumimoto Introduction Reactive oxygen species (ROS) are conventionally regarded as inevitable deleterious byproducts of aerobic metabolism On the other hand, there exist...
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Báo cáo y học: "Reactive oxygen species induce expression of vascular endothelial growth factor in chondrocytes and human articular cartilage explants" pps
... expression of VEGF, VEGF-121 and VEGF-165 splice variants and VEGF receptors (VEGFR-1 and VEGFR-2) after stimulation by ROS donors Knowledge of this expression could lead to a better understanding of ... Stimulation of immortalized chondrocytes (a) and articular cartilage explants (b) with PMA (μg per ml of medium) or SIN-1 (μM) The level of NO is increased after stimulation with reactive oxygen species ... biochemistry and molecular biology Edited by: Burdon RH Amsterdam: Elsevier; 1991:20 Henrotin YE, Bruckner P, Pujol JP: The role of reactive oxygen species in homeostasis and degradation of cartilage...
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Bone Metabolism: The Role of STAT3 and Reactive Oxygen Species
... Form 20 (Revised 9/10) PURDUE UNIVERSITY GRADUATE SCHOOL Research Integrity and Copyright Disclaimer Title of Thesis/Dissertation: Bone Metabolism: The Role of STAT3 and Reactive Oxygen Species For ... this work is free of plagiarism and all materials appearing in this thesis/dissertation have been properly quoted and attributed I certify that all copyrighted material incorporated into this ... STAT3 AND REACTIVE OXYGEN SPECIES A Thesis Submitted to the Faculty of Purdue University by America Bethanne Newnum In Partial Fulfillment of the Requirements for the Degree of Master of Science...
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Báo cáo y học: " Helicobacter pylori induces mitochondrial DNA mutation and reactive oxygen species level in AGS cells"
... Mitochondria are the centre of energy metabolism in the cell and a major source of ROS The proportion of oxygen converted into O2•ˉaccounts for about 1-2 % of the overall oxygen consumption [8] Mitochondrial ... region of AGS cells, the section of black line is the normal sequence; b: Sequence of 514CA repeats in mtDNA D-loop region of AGS cells stimulated by Hp extracts, and the section of black line is ... induces genomic instability in nuclear CA repeats in mice and in mtDNA of AGS cells and chronic gastritis tissue, and this effect in mtDNA is associated with bacterial virulence Furthermore, the...
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Báo cáo khoa học: Neuroglobin and cytoglobin expression in mice Evidence for a correlation with reactive oxygen species scavenging doc
... showed that Cygb is significantly upregulated upon 48 h of hypoxia, and that this increase is dependent on the tissue and the severity of hypoxia The mechanism of induction of Cygb is regulated by ... function of Ngb and on the mechanism of cell death after hypoxic exposure Results and Discussion As can be seen in Fig 1, the mRNA upregulation is dependent on the hypoxia exposure time and is tissuespecific ... regulated by HIF-1 The aim of this study was to illustrate the time dependency and the tissue specificity of Cygb and Ngb mRNA upregulation upon hypoxia and after reoxygenation In addition, we...
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Báo cáo khoa học: Is there more to aging than mitochondrial DNA and reactive oxygen species? pot
... further after oxidative stress The model is consistent with the observations of Yakes and van Houten [107], who found that oxidative stress promoted a higher incidence of polymerase-blocking strand ... levels of ROS The ‘vicious cycle’ Polgexo)/) mice and the existence of the ‘vicious cycle’ The main premise of the ‘vicious cycle’ hypothesis is the existence of a feed-forward cycle of ROS production ... of Ni(II) and Cu(II) on DNA interaction with the N-terminal sequence of human protamine P2: enhancement of binding and mediation of oxidative DNA strand scission and base damage Carcinogenesis...
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Báo cáo Y học: Inactivation of the Na+-translocating NADH:ubiquinone oxidoreductase from Vibrio alginolyticus by reactive oxygen species pot
... cell rupture and preparation of membrane vesicles of V alginolyticus cells were performed under exclusion of dioxygen In addition, the effect of enzymes that detoxify reactive oxygen species (superoxide ... Quantification of the EPR signals was carried out by comparison with a Cu2+ standard [13] RESULTS AND DISCUSSION the presence of NADH in the crude extract In order to test this hypothesis, French ... NADH oxidation and Q reduction activities of 2.45 and 0.99 lmolÆmin)1Æmg)1, respectively [15] Inhibition and uncoupling of the Na+-NQR activity by dioxygen Characterization of redox cofactors in...
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báo cáo khoa học: "Reactive oxygen species-mediated apoptosis contributes to chemosensitization effect of saikosaponins on cisplatin-induced cytotoxicity in cancer cells" pdf
... to cisplatin-induced cell death Saikosaponins and cisplatin co-treatment potentiates apoptosis in cancer cells Cisplatin can induce two distinct modes of cell death, apoptosis and necrosis, in ... intra- and inter-strand adducts with DNA, thus is a potent inducer of cell cycle arrest and apoptosis in most cancer cell types[10] However, a major limitation of cisplatin chemotherapy is that ... as indicated in (A), and then stained with annexin V and PI followed by flow cytometry analysis Early apoptosis is defined by Annexin V+/PIstaining (Q4) and late apoptosis is defined by Annexin...
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Báo cáo y học: " Early The tripeptide feG regulates the production of intracellular reactive oxygen species by neutrophils" pps
... this influx of leukocytes in antigen-challenged Brown Norway rats [6], and the peptide is also potent inhibitor of human and rat neutrophil adhesion and migration [10,17,18] The primary role of ... Mathison R, Davison JS, Befus AD: Neuroendocrine regulation of inflammation and tissue repair by submandibular gland factors Immunol Today 1994, 15(11):527-532 Rougeot C, Rosinski-Chupin I, Mathison ... 267(5 Pt 1):G745-53 Mathison R, Lo P, Moore G, Scott B, Davison JS: Attenuation of intestinal and cardiovascular anaphylaxis by the salivary gland tripeptide FEG and its D-isomeric analog feG Peptides...
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Báo cáo khoa học: "A Reactive oxygen species: toxic molecules or spark of life" docx
... intracellular mechanisms In this paradigm, ROS are not just random destructive species but regulators of metabolic processes and part of the chemistry of life [10,11] Furthermore, evidence of oxidative ... and creates a chain reaction that alters the fluidity and shape of the membrane This is the same process that makes oil rancid A consequence of the change to the cell membrane is disruption of ... O2•– is still not a very reactive radical; in the presence of H+ or HO2• it can reduce O2•– to H2O2 or be oxidized to O2 Another term that is often used is reactive oxygen species (ROS) This...
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Báo cáo y học: "Endogenous TGF-β activation by reactive oxygen species is key to Foxp3 induction in TCR-stimulated and HIV-1-infected human CD4+CD25- T cells" docx
... representative histogram profile of DHE staining on the different days The filled histogram is the un-labeled cells (negative control) B The values are displayed as the Mean ± SD of the MFI of DHE between ... representative FACS profile of cell size (A), DHET cells is displayedand apoptotic cells (C) between anti-CD3 plus antiFigure A representative FACS profile of cell size (A), DHE fluorescence (B) and apoptotic ... Brody AR: Asbestos-derived reactive oxygen species activate TGF-beta1 Lab Invest 2004, 84:1013-1023 Hildeman DA: Regulation of T-cell apoptosis by reactive oxygen species Free Radic Biol Med...
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