progenitor cells and the perivascular niche
Báo cáo y học: "Sequestration and homing of bone marrow-derived lineage negative progenitor cells in the lung during pneumococcal pneumonia" ppsx
... investigated the localization of the labeled donor cells in the pneumonia lungs and detected migration of these cells into alveolar air spaces In the whole BMC transfusion model (Figure 6A), the percentage ... hours) in the lung while the Lin- PCs were preferentially retained (24 hours) there during pneumococcal pneumonia Furthermore, these progenitor cells also remained in the lung tissues and rarely ... as well as the participation of progenitor cells in the repair response of the lung [18-21] We propose that during infectious inflammation of the lung, these BM-derived progenitor cells could...
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Vascularised bone tissue engineering endothelial progenitor cells and human mesenchymal stem cells coculture in 3d honeycomb scaffolds and the effect of bi rotational bioreactor and hypoxic microenvironment 1
... Prof Teoh Swee-Hin and A/Prof Jerry Chan I would like to thank them for their constant sharing of knowledge and ideas, their patience and understanding, and the many hours they have each dedicated ... 50 2.8.2.5 Endothelial Cell Types 51 2.8.2.6 Endothelial Progenitor Cells 52 2.8.2.7 Endothelial Progenitor Cells in Fracture Healing 53 2.8.2.8 Proposed Endothelial Cell Type ... endothelial cells and its progenitor cells Table 2-10 Comparison between UCB and adult PB-EPC in terms of its colony emergence, growth and vessel forming ability Table 2-11 Comparison of the...
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Stem CELLS and the FUTURE OF REGENERATIVE MEDICINE pdf
... fat cells, various brain and nervous system cells, insulin-producing cells of the pancreas, bone cells, hematopoietic cells, yolk sac, endothelial cells, primitive endodermal cells, and smooth and ... stem cells In the first, stem cells themselves are implanted in a diseased or injured organ in the hope that they will give rise to the mature cells needed by that organ In the second, the stem cells ... millimeter) The outer layer of the blasotocyst is called the trophoblast, and the cluster of cells inside the sphere is called the inner cell mass At this stage, there are about 70 trophoblast cells and...
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Tế bào gốc và ứng dụng trong y sinh học (Stem cells and the application in biomedicine) potx
... human embryonic blood stem cells Blood 96, 1740 - 1747 IItskovitz - Eldor, J and et al (2000).; Differentiation of human embryonic stem cells into embryoid bodies comprising the three embryonic germs ... A., et al (1998); Embryonic stem cells lines derived from human blastocysts Science 282, 1145 - 1147 12 Raisman, G., (2001); Olfactory ensheathing cells - another miracle cure for spinal cord ... - 822 Jones, G M., et al (2000); Human embryonic stem cells technology Semin Reprod Med 18, 219 - 223 Orlic D et al (2001); Bone marrow cells regenerate infarcted myocardium nature 410, 701 -...
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Báo cáo hóa học: " Nutraceutical augmentation of circulating endothelial progenitor cells and hematopoietic stem cells in human subjects" potx
... with the total number of cells counted cells being 30,000 per sample The percentage of CD133 and CD34 positive cells was calculated based on the measured number of leukocytes (CD45-positive cells) ... presented as percentage of mononuclear cells in Additional File Analysis of the number of the progenitor cells in circulation by HALO assay Cells expressing the CD34 and CD133 markers are associated ... Hematopoietic stem cells at various stages of differentiation are localized in the bone-marrow At a basal rate low levels of stem /progenitor cells are released from their niche and circulate in the peripheral...
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Báo cáo sinh học: " No relationship between the distribution of mast cells and the survival of stage IIIB colon cancer patients" pdf
... All the patients underwent radical surgery, and none of them had undergone either chemotherapy or radiotherapy before the collection of the samples The histopathologic characteristics of the ... staining ZJ and ZQ analyzed the results ZXS conceived the study, participated in the study design, and coordinated the writing and helped draft the manuscript All authors read and approved the final ... examine the distribution of mast cells and its relationship with the progression of colon cancer to identify the role of mast cells in this process Therefore, the current study examined the mast...
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báo cáo hóa học:" No relationship between the distribution of mast cells and the survival of stage IIIB colon cancer patients" potx
... All the patients underwent radical surgery, and none of them had undergone either chemotherapy or radiotherapy before the collection of the samples The histopathologic characteristics of the ... staining ZJ and ZQ analyzed the results ZXS conceived the study, participated in the study design, and coordinated the writing and helped draft the manuscript All authors read and approved the final ... examine the distribution of mast cells and its relationship with the progression of colon cancer to identify the role of mast cells in this process Therefore, the current study examined the mast...
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Báo cáo sinh học: " CNS progenitor cells and oligodendrocytes are targets of chemotherapeutic agents in vitro and in vivo" pdf
... reveals that the great majority of TUNEL+ cells in the SVZ and DG are doublecortin+ (DCX+) neuronal progenitors [44], and that other TUNEL+ cells include GFAP + cells (which may be stem cells or ... The progenitor cells that lie between NSCs and differentiated cell types, and are the major dividing cell population in the CNS, appear to be exceptionally vulnerable to the effects of chemotherapeutic ... cells caused 70-100% reduction in viability of O-2A/OPCs, GRP cells, NRP cells, and NSCs (Figure 3) The preferential vulnerability of both rat and human primary CNS progenitor cells to BCNU and...
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Báo cáo y học: "A paragon of self-tolerance: CD25+CD4+ regulatory T cells and the control of immune responses" pps
... Unfractionated CD4+ cells (which contain CD5high-expressing cells) prevented the induction of autoimmunity when transferred together with the CD5low cells, implying that the TR cells were contained ... still remain between the surface phenotype of TR and primed T cells, but they are more relative than absolute For example, although both primed T cells and TR cells express CD25, the latter does so ... with immature dendritic cells) As yet it is unclear whether de facto Foxp3+ TR cells can be generated in the periphery or whether the Tr1 cells produced from conventional Th0 cells are equivalent...
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Báo cáo y học: " Lentiviral transduction of Tar Decoy and CCR5 ribozyme into CD34+ progenitor cells and derivation of HIV-1 resistant T cells and macrophages" pdf
... differentiation of end stage cells such as T cells and macrophages [15] On the contrary, lentiviral vectors appear not to have these limitations [16,17] Based on these advantages, we used the new generation ... of CD34+ cells into macrophages and T lymphocytes We also show that the transgenic cells display resistance to HIV-1 challenge Results The regulatory proteins Tat and Rev encoded by the viral ... mouse based on the variable sizes of the grafts injected and possibly due to the varying numbers of true stem cells present in the samples injected [22,23] Since vector transduced CD34+ cells gave...
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Báo cáo y học: "A crucial role for tumor necrosis factor receptor 1 in synovial lining cells and the reticuloendothelial system in mediating experimental arthritis" pps
... cages The animals were fed a standard diet with food and water ad libitum All in vivo studies complied with national legislation and were approved by the local authorities on the care and use ... (1:200) and CD4-APC (1:100) or their respective isotype control antibodies Cells were washed and consecutively fixed and permeabilized using cytofix/cytoperm solution (BD Biosciences) Thereafter, cells ... IL-6 and the acute phase gene Saa1 To study the effects of TNFR1 silencing in spleen, we performed FACS and qPCR analyzes on the splenocytes (Figure 6b, c) and cytokine measurements on the APC...
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Báo cáo y học: " Fibrocytes and the tissue niche in lung repair" docx
... ECM forms the basement membrane located under the epithelial and endothelial cells The main components of the ECM are collagens, proteoglycans, hyaluronan, and other glycoproteins The ECM functions ... involved The structural cells involved in asthma include epithelial cells, smooth muscle cells, and (myo)fibroblasts The fibrosis, and for asthma characteristic tissue niche, is subepithelial There ... rich in ECM but with few cells The cells in the fibroblast foci are arranged in an outstretched and parallel arrangement relative to the other cells and parallel to the alveolar septa [29] (Figure...
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Báo cáo y học: "Current views concerning the influences of murine hepatic endothelial adhesive and cytotoxic properties on interactions between metastatic tumor cells and the liver" pptx
... tumor cells Endothelial-tumor cell interactions induce nitric oxide release from the hepatic endothelium Direct and indirect evidence from the literature has supported the hypothesis that the hepatic ... binding and stimulation of angiogenesis, and on the other hand, endothelial cells can contribute to host anti-metastatic responses by producing the cytotoxic molecule NO from arginine with the help ... system, the cellular origin of the NO release following B16F1 cell arrest in the liver has been identified as periportal endothelial and sinusoidal lining cells, and hepatocytes adjacent to the arresting...
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Báo cáo y học: " Effect of erythropoietin on level of circulating endothelial progenitor cells and outcome in patients after acute ischemic stroke" ppsx
... Barthel Index at 48 h after acute IS (Figure 2) On the other hand, a significant negative correlation was noted between the circulating level of EPCs (E and E3) and both modified Ranking Scale and ... dysfunction [35] Therefore, our results strengthen the findings of previous studies [33-35] Taken together, the results of the present study not only corroborate that of our recent report [24], they also ... contributions HKY and THT designed the experiment, drafted and performed laboratory work CKS and SL were responsible for the laboratory assay and troubleshooting HSL, SFC, CMY, TYT and MYL supervised...
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Báo cáo y học: "Analysis of proteomic profiles and functional properties of human peripheral blood myeloid dendritic cells, monocyte-derived dendritic cells and the dendritic cell-like KG-1 cells reveals distinct characteristic" doc
... mDCs and moDCs [12] and moDCs and KG-1 cells [13], but no studies have compared the proteomes of all three cell types In this study we compare the proteomes of mDCs, moDCs and KG-1 cells, and then ... molecules and migration to the lymph nodes During their maturation, DCs lose their antigen-capturing capacity and become mature immuno-stimulatory cells that have the ability to activate naïve T cells ... Dendritic cells (DCs) are highly specialized antigen presenting cells that originate from bone marrow progenitor cells They represent a major cellular component of the innate immune system and their...
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Distinct histone deacetylases repress expression of LH and FSH beta genes in the immature gonadotrope alphat3 1 cells and the repression is reversed by GNRH
... hormone (FSH) The pituitary gland is a small gland located at the base of the brain, functionally linked to the hypothalamus It is divided into two lobes: the anterior or front lobe and the posterior ... between the hypothalamus and pituitary gland The anterior pituitary gland is functioning connected with the hypothalamus; nerve cells in the hypothalamus secrete neurohormones that act on the endocrine ... synthesized in and released from the hypothalamus, binds to GnRH receptors on the surface of the gonadotrope This leads to the synthesis and secretion of LH and FSH, which stimulate the production...
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Báo cáo y học: "The change and effect of endothelial progenitor cells in pig with multiple organ dysfunction syndromes" ppt
... anatomical landmarks were marked on the animal's skin, including the cartilage thyroidea, the articulatio sternoclavicularis, the acromion, the inferior scapular angle, the pubic symphysis and the anterior ... stroke output and the central venous pressure A retention catheter was intubated into the right femoral artery for exsanguination and all catheters were fixed The skin of the animals was then sutured ... AM in HBSS and the plates were incubated for 30 minutes at 37°C, 5% carbon dioxide The labeling solution was removed and the plates washed twice, and we then counted the tubes in the plate using...
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Báo cáo y học: "ntravenous transplantation of allogeneic bone marrow mesenchymal stem cells and its directional migration to the necrotic femoral head"
... culture, the medium was refreshed, and then the medium changed every other day Cell passaging was performed when cell confluence reached 85% The purity and immunophenotype of MSCs and their potentials ... ischemic, stem cells in circulation are adherent to the vascular endothelial cells, cross the endothelial cells, migrate and finally reached at ischemic sites Inflammatory may be observed in the local ... smooth and round, and the articular cartilage was transparent and glossy (Figure 5A) After surgery, the shape of femoral head was not markedly changed and the surface of femoral head was pale and...
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Tài liệu Báo cáo khoa học: Development of a new method for isolation and long-term culture of organ-specific blood vascular and lymphatic endothelial cells of the mouse pdf
... problem Therefore, the isolation and maintenance of murine endothelial cells from various developmental stages and locations is important for dissecting molecular and cellular mechanisms of endothelial ... cells as well as ECs [27], and that cardiac valvular cells were derived from endothelial cells through an endothelial-to-mesenchymal transition during early development [33] The presence of these ... cells and embryonic endothelial cells may be contaminants in the present culture system In order to overcome these disadvantages, the use of tamoxifen-inducible Cre-expressing mice, such as the...
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Tài liệu Báo cáo Y học: The Fc receptor c-chain is necessary and sufficient to initiate signalling through glycoprotein VI in transfected cells by the snake C-type lectin, convulxin ppt
... illustrates the dual mechanism of the complex, with one subunit, namely GPVI, responsible for binding to the ligand, and the other subunit, the FcR c-chain, responsible for transmission of the signal ... K562 cells Cells stably expressing wild-type GPVI and the FcR c-chain and differentiated with PMA for 24 h were stimulated with the indicated concentrations of collagen and CRP for 90 s, and Syk ... other FcRs [1,3,17] The similarity between GPVI and different FcRs provides a starting point in the study of the structure–function relationship of GPVI and the signalling pathways Many FcRs and...
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