principles techniques and applications second edition
Báo cáo y học: "Sequence similarity between the erythrocyte binding domain 1 of the Plasmodium vivax Duffy binding protein and the V3 loop of HIV-1 strain MN reveals binding residues for the Duffy Antigen Receptor for Chemokines" ppsx
... RANTES and MIP-1a and between AOPRANTES and MIP-1a, but not between RANTES and AOP-RANTES (p < 0.05) Blocking of P knowlesi invasion of DARC+ human erythrocytes by RANTES and AOP-RANTES A standard ... AAC TGT AAT TAT3’ and 5’CAA ATT GGG CCC TTC CTT CAT ACA TAA TTG3’ and contain the restriction sites for Apa I and Pvu II The pHVDR22 plasmid was digested with Apa I and Pvu II, and the digested ... conceived of the study, and RFG participated in its design and coordination and helped to draft the manuscript Both authors read and approved the final manuscript Bolton and Garry Virology Journal...
- 10
- 370
- 0
Báo cáo y học: " Systems-level comparison of host responses induced by pandemic and seasonal influenza A H1N1 viruses in primary human type I-like alveolar epithelial cells in vitro" pdf
... involved in the regulation of seasonal-, pandemic- and sharedresponse genes Of the 13 transcription factors regulating genes affected by both seasonal and pandemic viruses, four (IRF1, IRF2, IRF7, ... between the pandemic and seasonal H1N1 viruses lay in their ability to potentially alter host transcriptional and translational responses Down-regulation of zinc finger proteins and small nucleolar ... Authors’ contributions SMYL, RWYC, MCWC and JSMP conceived and designed the experiments RWYC, MCWC and SSRK generated the type I-like alveolar epithelial cells and performed the virus infection experiments...
- 9
- 359
- 0
The transference of meaning through class of words denoting parts of the human body in english and vietnames
... human body in English and Vietnamese Choosing two languages, English and Vietnamese to make a contrastive analysis has scientific base on linguistics and culture England and Vietnam have their ... Furthermore, because of the importance and necessity of substratum knowledge in teaching and learning English and Vietnamese, we would like to examine and find out some relationships between ... transference of English and Vietnamese words to help teaching and learning English and Vietnamese as well We hope the results of the study will help the readers have deep understanding about the mechanism...
- 69
- 794
- 4
Tài liệu Báo cáo khoa học: Multidentate pyridinones inhibit the metabolism of nontransferrin-bound iron by hepatocytes and hepatoma cells docx
... effects of DFP and novel tetradentate and hexadentate analogues on NTBI uptake and mobilization from rat hepatocytes and hepatoma cells DFO was included as a reference chelator Materials and methods ... described in Materials and methods Results are the mean and standard deviation of iron internalized by normal and iron-loaded hepatocytes from three separate experiments, and are expressed as a ... 4LI-Me-3,2-HOPO (4L1) and 5LIOMe-3,2-HOPO (5L1O) and the bidentate deferiprone (DFP) Protein purification Ferritin was isolated using the method of Huebers and colleagues [15] and used to raise an...
- 10
- 545
- 0
Tài liệu Báo cáo khoa học: Oxygen-dependent regulation of hypoxia-inducible factors by prolyl and asparaginyl hydroxylation pdf
... (HPHs) 3, and 1, respectively [94]), and one HIF asparaginyl hydroxylase enzyme called factor-inhibiting HIF-1 (FIH-1) [88,95] were identified and shown to hydroxylate the key proline and asparagine ... succinate and CO2 (Fig 2) Therefore, the rapid turnover and transcriptional silencing of the HIFa protein subunits involves oxygen-dependent prolyl and asparaginyl hydroxylation by the PHD/ HPHs and ... (normoxia) the asparaginyl and HIF prolyl hydroxylases (FIH-1 and PHD/HPH) hydroxylate (OH) HIFa on specific asparagine (Asn) and proline (Pro) residues, blocking transactivation and targeting HIFa for...
- 10
- 603
- 0
Tài liệu Báo cáo Y học: Regulation of transcription of the Dnmt1 gene by Sp1 and Sp3 zinc finger proteins doc
... different roles in the activation and repression of transcription [9] In our hands, Sp1 generated a single band and Sp3 generated two bands in EMSAs (two of three bands migrated to the same position; ... to the manufacturer’s protocol Cell culture, transfections and assays of promoter activity Mouse NIH3T3 and F9 cells and human HeLa and 293 cells were grown in Dulbecco’s modified Eagle’s medium ... against Sp1, Sp3 and AP-2 (Fig 3A) The retarded band designed B1 was shifted even further upon addition of antibodies against Sp1 (lane 3), while the retarded bands corresponding to B2 and B3 were...
- 10
- 563
- 0
Báo cáo khoa học: Fluorescence quenching and kinetic studies of conformational changes induced by DNA and cAMP binding to cAMP receptor protein from Escherichia coli ppt
... i 3ị where F0 and F are uorescence intensities in the absence and presence of quencher, Q, respectively, Ki and Vi are the dynamic and static quenching constants, respectively, and fi is the ... of the CRPW85A mutant and its complexes with cAMP and DNA were conducted using an excitation wavelength equal to 295 nm Phase and modulation were analyzed by using single- and double-exponential ... M)1 and a fraction f1 ẳ 0.81; and ( ) the unquenchable component with a maximum at about 337 nm, characterized by an average value of KSV2 ẳ 0.00 M)1 and a fraction f2 ẳ 0.19 high afnity and...
- 14
- 400
- 0
Báo cáo Y học: High affinity binding between laminin and laminin binding protein of Leishmania is stimulated by zinc and may involve laminin zinc-finger like sequences doc
... alkaline phosphatase conjugated secondary antibody whereas indirect immunoblotting denotes sequential treatment with laminin, anti-laminin Ig and secondary antibody MATERIALS AND METHODS Anti-LBP Ig ... Fig Isolation and identification of LBP L donovani membrane proteins isolated by biotinylation and streptavidin–agarose extraction and passed through DEAE-cellulose, Con A–Sepharose and laminin– ... with avidin probes also did not reveal any band (lane 4) Direct immunoblotting using anti-LBP Ig and secondary antibody also resulted in a 67-kDa band (lane 5) confirming the authenticity of the...
- 8
- 420
- 0
Báo cáo khoa học: Substrate specificity of the human UDP-glucuronosyltransferase UGT2B4 and UGT2B7 Identification of a critical aromatic amino acid residue at position 33 doc
... as androsterone and b-estradiol (Fig 4B), and greatly reduced the activity towards HDCA and 4-hydroxyestrone (Fig 4B, Table 1) In addition, glucuronidation of bulky phenols, 4-hydroxybiphenyl and ... loading onto the gel 2, 10 and 100 lg of membrane proteins for UGT2B4, UGT2B4F33L and UGT2B4F33Y, respectively, and 12, 15 and 15 lg of membrane proteins for UGT2B7, UGT2B7Y33L and UGT2B7Y33F, respectively ... various steroid hormones (androsterone, epitestosterone) and fatty acids [7] UGT2B4 and UGT2B7 therefore play a key role in the detoxification of cholestatic bile acids and may prevent the formation...
- 9
- 343
- 0
Find-A-Ride: A listing of TLC Licensed Bases by Borough and by Zip Code pdf
... B01721 Z AND Z TRANSPORTATION INC 59 CANAL STREET 917-330-6936 Luxury Limousine B02451 CITI COACH NYC, INC 551 GRAND STREET SUITE 212-777-1277 Paratransit B90663 TRIPWAY SERVICES 551 GRAND STREET ... KINGS HIGHWAY LUXOR LIMO INC 502 AVENUE U MINT CAR SERVICE INC 2510 CONEY ISLAND AVENUE OCEANA CAR AND LIMO 2395 CONEY ISLAND AVENUE PRESTIGE CAR SERVICE 387 AVENUE X TOV TOO TRANSP INC 1811 EAST ... EASTLAND CAR SERVICE 2982 NOSTRAND AVENUE 718-382-6868 Community Car B01509 CONCORDE EXP OF N.Y INC 1611 EAST 19 STREET 718-645-5555 Community Car B01751 EASYRIDE CAR SERVICE INC 2982 NOSTRAND...
- 70
- 615
- 0
Báo cáo Y học: Potential active-site residues in polyneuridine aldehyde esterase, a central enzyme of indole alkaloid biosynthesis, by modelling and site-directed mutagenesis ppt
... (His)6PNAE and 10 muteins of it Four of six cysteine residues in positions 20, 132, 170 and 257 were changed to alanine and one (position 213) to serine Histidine at position 17, 86 and 244, serine ... HgCl2 from Merck (Darmstadt, Germany) and diethylpyrocarbonate (DEPC) was from AppliChem (Darmstadt, Germany) All solvents and chemicals were of analytical grade and obtained from Merck (Darmstadt, ... (Km, kcat) were completed for the active muteins and for the wild-type enzyme Enzyme and protein assays Protein concentrations of the muteins and the wild-type enzyme were determined with the...
- 8
- 345
- 0
Báo cáo khoa học: Glycation and glycoxidation of low-density lipoproteins by glucose and low-molecular mass aldehydes Formation of modified and oxidized particles pot
... artefactual oxidation during such sample handling and analysis [23,24] Samples (80 lL) were removed from the LDL incubations and 0.1 vol each of 20 mM EDTA and 0.2 mM BHT and 670 lL borate buffer (pH > ... 205 and 234 nm and a RF-10AXL fluorescence detector (for TOH; kex 290 nm and kem Ó FEBS 2003 3574 H M Knott et al (Eur J Biochem 270) 330 nm) Metabolites were quantified by comparison with standards ... incubations and loaded onto precast 1% (w/v) agarose gels Native and acetylated LDL (Ac-LDL, prepared as described previously [25]) were loaded as negative and positive controls, respectively, and the...
- 11
- 401
- 0
recursive models of dynamic linear economies by hansen and sargent 2005 (526 pages)
... summarized by Harris (1987) and Stokey, Lucas, and Prescott (1989) See Sims (1980), Hansen and Sargent (1980, 1981, 1990) For example, see Kwakernaak and Sivan (1972), and Anderson and Moore (1979) See ... motion, and retains calculation of equilibria and asset prices via simple modifications of our standard formulas These preferences are a version of those studied by Epstein and Zin ( ) and Weil ... a set of matrices A22 , C2 , Ub , and Ud that characterize the motion of information sets and of taste and technology shocks; matrices Φc , Φg , Φi , Γ, ∆k , and Θk that determine the technology...
- 526
- 238
- 0
Báo cáo sinh học: " Purification of infectious human herpesvirus 6A virions and association of host cell proteins" pot
... contributions MH and JA carried out the purification and analysis of the study with equal contribution and drafted the manuscript AF-H, SJ and HG participated in its design and coordination and helped ... shown Results are based on three experiments and presented as mean ± standard deviation (error bars) B and C Light microscopic analyses of HHV-6A- and mock-infected JJHAN cells at dpi Note the ... in HHV-6A infected cells D and E Detection of HHV-6 antigen gp60/110 in HHV-6A infected cells HHV-6A -and mockinfected cells were fixed and stained for gp60/110 (red) and counterstained with DAPI...
- 11
- 374
- 0
báo cáo hóa học:" Biomechanical and system analysis of the human femoral bone: correlation and anatomical approach" docx
... measurement of the linear and angular parameters and their applications (B) The special tool (A) for the one-moment measurement of the linear and angular parameters and their applications (B) value ... according to Wagner and colleagues [16] varies from approximately 125° up to 132° Furthermore, according to Nikitiuk and Ovsiankin [17], its size varies from 109° up to 153° and there is no angle ... determine the group and level of the geometric system base parameters, thus analyzing the femur structure on the basis of a complex and thorough investigation Methods Anatomical samples and analysis...
- 7
- 462
- 0
Báo cáo hóa học: " Purification of infectious human herpesvirus 6A virions and association of host cell proteins" potx
... contributions MH and JA carried out the purification and analysis of the study with equal contribution and drafted the manuscript AF-H, SJ and HG participated in its design and coordination and helped ... shown Results are based on three experiments and presented as mean ± standard deviation (error bars) B and C Light microscopic analyses of HHV-6A- and mock-infected JJHAN cells at dpi Note the ... in HHV-6A infected cells D and E Detection of HHV-6 antigen gp60/110 in HHV-6A infected cells HHV-6A -and mockinfected cells were fixed and stained for gp60/110 (red) and counterstained with DAPI...
- 11
- 359
- 0
báo cáo hóa học:" Quality of Life as reported by children and parents: a comparison between students and child psychiatric outpatients" ppt
... outpatient and student samples was found on the Total (M = 48.0, SD = 13.8 and M = 47.7, SD = 14.0), Externalizing (M = 14.4, SD = 7.2 and M = 14.5, SD = 7.4) and Internalizing (M = 13.4, SD = 8.0 and ... psychiatric outpatients and regular students recruited from the same catchment area and during comparable time intervals and matched for sex and age, we found outpatients to report, and be reported by ... Jozefiak et al Health and Quality of Life Outcomes 2010, 8:136 http://www.hqlo.com/content/8/1/136 Page of Table Outpatients and students matched by age, sex and levels of emotional and behavioural...
- 9
- 494
- 0
Báo cáo hóa học: " Research Article Monocular 3D Tracking of Articulated Human Motion in Silhouette and Pose Manifolds" ppt
... and Ωk are the mean and covariance matrix of the output of the kth expert Uk ≡ {Wk , Ωk } and U ≡ {Uk }K=1 Following [33], in our framework we consider k the joint distribution p(c, s | Ξ) and ... about seconds per frame BME inference, sampling, and sample weight evaluation is fairly fast, taking about 200 milliseconds per frame The total time to process one frame of input image is about seconds ... view (top row) and their estimated poses (the second and third rows) To show the effectiveness of the proposed framework, results from the static image estimation using only BME and results from...
- 18
- 383
- 0
Multi wall carbon nanotubes (MWCNTs) doped polypyrrole DNA biosensor for label free detection of genetically modified organisms by QCM and EIS
... and with a length of 1–2 m All aqueous solutions were prepared using Milli-Q water (18 M cm) 1165 Table Composition of PBS buffer and sequences of used ODN probe and targets (complementary and ... that of proteins and therefore DNA detection can be used for both raw and processed materials while protein detection is suitable only for raw materials) On the need of fast and sensitive analytical ... maize and the Flavr Savr tomato Thus, the 35S and NOS sequences are the most frequently used targets for GMO screening in DNA detection approach (this approach is more often used than the second...
- 6
- 275
- 2
Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ lồng sóc đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25