... mitochon-drial respiratory chain, the assistance of specificchaperone proteins is also required. The available dataindicate that the accessory factor Bcs1p is involved in the binding of ISP to an immature ... were of analytical grade.Yeast strains and growth media The genotypes and sources ofthe S. cerevisiae strains aredescribed in Table 2. The ISP deletion strain wasprepared in accordance with the ... seen inthe molecular mass ofthe bc1complex in these two deletion strains, thus leading to the hypothesisthat the addition of ISP may play a pivotal role in the structural rearrangement of the...
... [6,8,27] or toany other sequences available in FASTA and BLASTdatabase programs at the DNA Data Bank of Jap an.Recently, we reported the cloning and s equencing of the gene encoding 4-amino-3-hydroxybenzoate ... and2-aminomuconic acid inthe modified meta-cleavage path-way (Fig. 1B). The 2-aminomuconate deaminase from s trainAP-3 and that from strain JS45 have been purified andcharacterized in detail [5,6]. The nucleotide ... not have anabsorbance peak at 300 nm [5]. A cofactor is not requiredfor t he enzyme activity. In contrast, the deaminase fromstrain 10d contained an FAD-like cofactor, similar toD-amino acid...
... consisting of amino acids 7–22 of parabutoporin (an a- helical part having the four amino acids LAKK identical to mastoparan) and of the first 28 amino acids ofthe opistoporins were synthesizedand ... being least active againstS. marcescens. Mastoparan is far less active in inhibitinggrowth of Gram-negative bacteria and melittin is only activeagainst three ofthe Gram-negative bacteria at ... parabutoporin, this could explain the higher antibacterial activity on Gram-negative bacteria forparabutoporin compared to opistoporin 1. Also with mag-ainin analogs, an increase in antibacterial activity...
... [11]. MS analysis has indicated the presence of a phosphate group in several other strains [12–14], but,owing to the low abundance ofthe phosphate-containingglycoforms, its location has not ... toencode a sialyltransferase that adds Neu5Ac in an a- 2,3-linkage to lactose in other H. in uenzae strains, is present in strain 981 (data not shown). Sialyllactose is known tocontribute to the ... and pneumonia) has been greatlyreduced in recent years as a result ofthe development of conjugate vaccines, there exists no vaccine against nontype-able H. in uenzae (NTHi). NTHi strains are...
... Action]AFP Australian Federal PoliceAG Attorney-GeneralAIC Australian intelligence communityANAO Australian National Audit OfficeAQMI Al-Qaida pour le Maghreb Islamique [al Qaeda for the Islamic ... Islamic Maghreb]ASALA Armenian Secret Army for the Liberation of ArmeniaASIO Australian Security Intelligence OrganisationASIS Australian Secret Intelligence ServiceAUSTRAC Australian Transaction ... and Canada, ASIO derives much of this information from human sources. A certain amount of data emanates from well-placed informants and individuals who submit plea bargains during trials for...
... preliminary human and animal trials have shown it to be effective in treating osteoarthritis (OA). The present clinical trial evaluated the safety and efficacy of UC-II as compared to a combination ... collagen extracts contain the amino acids found inthe framework of human cartilage. In addition, these amino acids are required for the synthesis and repair of connective tissue throughout the ... currently available for OA, individualized treatment programs are available to help relieve pain and stiffness, and to maintain and/or improve func-tional status. Inthe last few years, various...
... of variables. One ofthe underlying objectives of EFA is to reduce a large number of variables to a minimum number that can explain most ofthe characteristics ofthe original variables. Each ... extracted from the original set of variables represents a discriminated aspect ofthe construct being studied. These extracted variables then can be used as independent variables in later analysis. ... quality is a component ofthe original model of CBBE; however, banking is a service-dominat industry and all banking products, as termed inthe industry, are actually services or packages of...
... developed industry. Within the American society, there are many races such as white, black or African-American, American Indian or Alaska native, Asian, native Hawaiian, other Pacific Islander and ... practices ofthe young American in general and the young American women in particular of living together before marriage. All ofthe characters inthe story are intellectual: Paul, the protagonist ... American informality discussed above in their greeting, in welcoming guests or in behaving as a guest, we have seen many informal practices in their dressing such as the Casual Friday in many American...
... aninternational transaction. Unless parties are assured that the coverage is available in the amount designated, the failure ofa party to obtain insurance coverage shouldnot be grounds for termination of ... is a certain moment of time wherein the ownership ofthe goods by the seller ceases and passes to the others and that of the buyer begins; that is called the transfer of title. Ina certain aspect, ... between the involved parties. It can be seen as an obvious offer ofthe Seller to the Company in case of any goods damage arising out ofthe Company’s obligations such as damageduring shipment, damage...
... best candidate for t he binding of the a- carboxylate group ofthe s ubstrate, when the external aldimine is formed. The anchoring of a- carboxylateand a- amino group inthe external aldimine definesautomatically ... definesautomatically the positions ofthe a- proton and the sidechain of any bound amino ac id. The lability ofthe a- protonobserved for a large number of amino acids [5] under the action of TPL ... groups of amino acid inhibitors mentioned above. The interaction of L-phenylalanine,L-methionine, andtheir a- deuterated analogs with TPL in D2O was charac-terized by the appearance of quinonoid...
... when the rate of amylopectin synthesisbecomes minimal. At these moments the rates of amylopec-tin synthesis are only 5% of their maximal values. In fact thislow rate of amylopectin polymerization ... GBSSI-defective strainBAFR1 (mt+ nit1 nit2 sta2–29::ARG7) contains a disrup-tion ofthe STA2 gene that was generated through randomintegration ofthe pARG7 plasmid inthe nuclear DNA of C. reinhardtii ... reference and a mutant strain carrying a gene disruption inthe STA2 locus of C. reinhardtii (strainIJ2). This sta2-29::ARG7 mutation induces the simulta-neous loss of GBSSI activity and ofthe major...
... that we can always perturb a quadratic map and change its topological type if it is not an Axiom A map.We want to do the same with an arbitrary unimodal map of an interval. So the first reasonable ... mapped inthe 2-to-1 way onto the domain A (so that there is a criticalpoint of g inthe central domain),• All other components of B are mapped univalently onto A by the map g,• The iterates ... map onto A 1.This map is again a real holomorphic box mapping and we can again consider the first return map onto the domain A 2(which is a central domain ofthe firstentry map onto A 1) and...
... and bacterial, proteasecontaminationProtein concentration was determined with a Bio-Rad pro-tein assay kit using BSA as a standard according to the manufacturer’s protocol. Any bacterial and ... database and search engine. Massdetermination of proteins was done with the same instru-ment operated in linear mode and externally calibrated with a mixture of proteins.Protein determination ... USA)and the tryptic peptides were extracted and analysed in a Bruker Ultraflex MALDI TOF ⁄ TOF instrument usingalpha-cyano-4-hydroxycinnaminic acid (Sigma) as matrix. The instrument was calibrated...
... According to the sequenceobtained, the b-subunit contains 535 amino acids with a calculated average molecular mass of 57 752 Da and the a- subunit contains 808 amino acids with a calculated averagemolecular ... AY082333).ResultsGene localization and sequence The observation ofa naturally occurring plasmid as part of the C. acidovorans genomic DNA [26] raised the question of location ofthe xdhAB operon. The plasmid size ... a functional Mo catalyticcenter. The functionality is estimated as a ratio of change in the absorbance at 450 nm after anaerobic reduction of the enzyme with 1 mMxanthine relative to the absorptionchange...